فهرس المقالات Somatic embryogenesis

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  1 - The effect of different hormone combinations on direct and indirect somatic embryogenesis in Agave americana
  Maryam Nazir Shahab Sadat Mehdi Soltani Howyzeh
  10.30495/ijpp.2019.664579
  Direct and indirect somatic embryogenesis in Agave Americana through basal parts of micropropagated shoots were considered in this study. For micropropagation, 2 factors (BA and NAA) were utilized in different concentrations. A factorial experiment based on a completely أکثر

  Direct and indirect somatic embryogenesis in Agave Americana through basal parts of micropropagated shoots were considered in this study. For micropropagation, 2 factors (BA and NAA) were utilized in different concentrations. A factorial experiment based on a completely randomized design (CRD) was accomplished with four replicates in a modified Murashige and Skoog medium (MSB). BA in 3 mg/L along with NAA in 0.1 mg/L was the best treatment for multiplication. Callus and embryo induction were evaluated using different auxins in a CRD experiment with three replicates in MS medium supplemented with L2 vitamins. Picloram 2.5 and 2 mg/L and also Dicamba 1 mg/L induced 66.7%, 60%, and 53.3% of explants to somatic embryo, respectively. Also, 2,4-D 2 mg/L had the highest impact on callus induction (95.6%). Embryogenesis was studied using Dicamba and Picloram in the same culture medium in a factorial experiment based on CRD with three replicates. The results of orthogonal polynomial analaysisindicated that high concentrations of Dicamba from 0 to 1.5 mg/L had a significant effect on somatic embryo induction. However, higher concentrations had the opposite effect on somatic embryo numbers. Germination of somatic embryos was performed in MSB medium without growth regulators. تفاصيل المقالة
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  2 - Some Useful Information about Micropropagation
  Behzad Kaviani
  This review article investigates a comprehensive description of the factors, methods, strategies, approaches and prerequisites influencing in vitro micropaopagation and the amount of their use across the world. The findings presented here are the results of the study of أکثر

  This review article investigates a comprehensive description of the factors, methods, strategies, approaches and prerequisites influencing in vitro micropaopagation and the amount of their use across the world. The findings presented here are the results of the study of several hundred papers published all over the world. Tissue culture techniques are routinely used for micropropagation. Enhancement of efficiency and efficacy of plant regeneration are primary goals of micropropagation. In this article, maximum and minimum use of some basic issues concerned with plant tissue culture in vitro especially micropropagation such as types of explants, types of culture media, types of sterilizing agent for explants, types of plant growth regulators (PGRs) for general studies of miro-propagation, shoot and root induction, somatic embryogenesis (SE) and callus induction have been considered. Maximal application for explants, culture media and sterilizing agent are single node, Murashige and Skoog (MS) and sodium hypochlorite (NAClO), respectively. BA and NAA are the most application among cytokinins (CKs) and auxins for general studies of miroprop-agation. Among all PGRs used for general studies of miropropagation, NAA is on the top. BA and IBA are the most use among CKs and auxins for shooting and rooting of explants, respectively. 2,4-D, NAA and TDZ are used more than the other PGRs for induction of SE. Among all types of auxins and CKs used as singular or in combination with them for callus induction, 2,4-D is at the top level. Combination of BA and NAA is the maximum for general studies of miropropagation. This review article can help to the future studies on micro-propagation due to the correct selection of the treatments تفاصيل المقالة