فهرس المقالات Polymorphic

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  1 - Novel Single Nucleotide Polymorphisms (SNPs) in Intron 2 and Exon 3 Regions of Leptin Gene in Sumba Ongole Cattle
  و.پ.ب. پوترا پ.پ. آگونگ
  The bovine leptin (LEP) gene was widely used as a candidate gene for molecular selection to improve productivity traits of cattle. This study was carried out to identify single nucleotide polymorphisms (SNPs) in the LEP gene of Sumba Ongole (SO, Bos indicus) cows using أکثر

  The bovine leptin (LEP) gene was widely used as a candidate gene for molecular selection to improve productivity traits of cattle. This study was carried out to identify single nucleotide polymorphisms (SNPs) in the LEP gene of Sumba Ongole (SO, Bos indicus) cows using sequencing method. A total of 31 animals were used in this study for analyses. Research showed that total of 16 SNPs were detected in the LEP gene. Along 2025 bp of LEP gene sequence was analyzed in this study and consisted of intron 2 (1002 bp) and exon 3 (1023 bp). The polymorphic informative content (PIC) value was reached from 0.06 (low) to 0.37 (moderate). Total of 16 SNPs in LEP gene of SO cattle had moderate PIC value (0.25<PIC<0.50) and consisted of twelve SNPs in intron 2 and four SNPs in exon 3. The SNPs with moderate PIC value were detected in intron 2 (g.2325G/T; g.2423A/C; g.2448C/T; g.2456C/G; g.2466C/T; g.2778T/A; g.2857G/A) and exon 3 (g.3260T/C; g.3272T/C; g.3356C/T; g.3468G/A). The SNP of g.3468G/A as the novel SNP in LEP gene of SO cattle that not reported in other breeds of cattle. This SNP was changed the amino acid from glycine (GGG) to arginine (AGG). Two type of mutation were detected in the LEP gene of SO cattle and consisted of transversions (44%) and transitions (56%). It was concluded that the LEP gene in SO cattle was showed polymorphisme and potential for molecular selection in the breeding program through depth research. تفاصيل المقالة
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  2 - Genetic diversity and relationships among traits in potato genotypes using agronomic traits and molecular marker (SSR).
  Hossein Afshari Ehsan Barzin Ghanbar laei Morteza Noryan
  10.30495/ijpp.2017.533563
  The molecular marker (SSR) has been used to investigate the markers associated with the agronomic traits including days to 50% flowering, tube ring time, days to maturity, plant height, the number of main stems per plant, the number of tubers per plant, dry matter conte أکثر

  The molecular marker (SSR) has been used to investigate the markers associated with the agronomic traits including days to 50% flowering, tube ring time, days to maturity, plant height, the number of main stems per plant, the number of tubers per plant, dry matter content, main stem diameter, a single tuber weight, average single tuber weight, and the total yield in potato genotypes. Ten primers used in 16 potato genotypes were investigated and principal component analysis and cluster analysis were used to determine genetic distance and genotype classification. A significant difference was found between all cultivars, indicating high genetic diversity for the traits under study, except for the diameter of stem trait. Yield showed a significant positive correlation with the number of tubers per plant, weight of tubers per plant, single tuber weight, and the number of main stems per plant. Special values resulted from components of 1-5 were higher than 1 in principal component analysis and totally justifying 76.70% of all variables variances. Molecular diversity of cultivars was measured using parameters of polymorphic information, the number of effective alleles, and Shannon index and the average of 39.03 SSR bands was achieved among which 9.41 bands were polymorphic. The average number of polymorphic bands varied for each primer from 1.56 to 2.12. Maximum and minimum polymorphic bands belonged to the HVM70 primer with 2.12 and the Bmacoo40 primer with 1.56, respectively. Also, the mean maximum band (4.4) and minimum band (3.1) belonged to Agra and Deyta cultivars, respectively. The morphologic data did not conform to the molecular data indicating that SSR marker had no genetic relationship with positions controlling the measured morphologic traits. Since the SSR markers were located in non-coding area of genome, no relationship between classifications of molecular markers and morphologic data was unexpected. تفاصيل المقالة
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  3 - Genetic Variation in Iranian Rice (Oryza Sativa L ) Genotypes Using Physiological traits and SSR markers
  Mehrnesa Qarehkhani Saeid Navabpour Hossein Sabouri Sanaz Ramezanpour
  10.30495/ijpp.2022.1893993.1206
  In order to assess genetic diversity, the number of 38 Iranian rice cultivars were evaluated using 15 agronomic traits and 14 SSR markers. Rice genotypes were cultivated in a Randomized Complete Block Design in a Research Farm located in Azadshahr in 2013. According to أکثر

  In order to assess genetic diversity, the number of 38 Iranian rice cultivars were evaluated using 15 agronomic traits and 14 SSR markers. Rice genotypes were cultivated in a Randomized Complete Block Design in a Research Farm located in Azadshahr in 2013. According to analysis of variance (ANOVA) genotypes showed a significant difference for all traits (P <0.01). Regression analysis showed that the weight of filled grains was correlated with panicle number and chlorophyll content and explained 93.5% of the filled grains weight variations. The highest and lowest content of polymorphic information was recorded in RM262 (0.81) and RM241 (0.33) respectively, the mean PIC was 0.51. The average Shannon index was 0.33. The genotypes were categorized according to morphological traits in three groups but based on molecular and chlorophyll data assigned to three and two groups, respectively. Among the microsatellite markers used in this study, the RM142 RM255, RM341, RM262 markers had a high degree of polymorphism. RM297, RM104, RM274 showed the most correlation with rice chlorophyll content, which can be promising in marker selection programs. تفاصيل المقالة