Micropropagation

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1 - مقایسه اثر واکشتها بر میزان موفقیت ریزازدیادی گیاه آناناس (Ananas comosus L. Merr)
سبحان گائینی فرح فراهانی فاطمه جمالو

چکیده :پیشرفت های علمی اخیر در زمینه بیوتکنولوژی گیاه آناناس روش های جدیدی را برای ریزازدیادی آناناس، تکثیر انبوه و کاهش هزینه های تولید فراهم ساخته است. تغییرات ژنتیکی همراه با ریزازدیادی ارقام جدیدی از گیاهان آناناس را بوجود آورده که از نقطه نظر اقتصادی، مقاومت به آفا أکثر

چکیده :پیشرفت های علمی اخیر در زمینه بیوتکنولوژی گیاه آناناس روش های جدیدی را برای ریزازدیادی آناناس، تکثیر انبوه و کاهش هزینه های تولید فراهم ساخته است. تغییرات ژنتیکی همراه با ریزازدیادی ارقام جدیدی از گیاهان آناناس را بوجود آورده که از نقطه نظر اقتصادی، مقاومت به آفات و بیماری های اساسی بعنوان مهمترین اهداف کاربرد بیوتکنولوژی آناناس هستند. در این پژوهش مراحل مختلف ریزازدیادی گیاه آناناس تحت تاثیر غلظت های مختلف هورمونی بررسی و مقایسه شدند. به منظور ریزازدیادی ، مریستم های مورد نظر از گیاه آناناس در شرایط استریل جدا شده و سپس آن را به محیط کشت موراشیگ اسکوگ (MS ) با غلظت های مختلف 6-بنزیل آمینوپورین ((1-2-3-4-5 Mgl نفتالین استیک اسید((6 Mglکشت داده شدند. با افزایش میزان هورمون 6-ینزیل آمینوپورین میانگین طول ساقه (2 سانتی متر) و نرخ تکثیر (3 عدد در هر جداکشت) بدست امد و میزان رشد افزایش پیدا کردند. کلمات کلیدی: گیاهچه آناناس – ریزازدیادی – تنظیم کننده های رشد تفاصيل المقالة

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2 - بهینه سازی ریزازدیادی گیاه دارویی آلوئه (Aloe littoralis) بومی ایران
آتنا بیانی فرح فراهانی زهرا نورمحمدی طاهر نژاد ستاری

آلوئه (Aloe littoralis) از نظر اقتصادی و دارویی اهمیت دارد. این گیاه بومی ایران تکثیر طبیعی و سنتی بسیار کند دارد. بنابراین، نمی تواند نیاز صنعت دارویی کشور را تامین کند. برای تکثیر سریع گیاه آلوئه (A.littoralis) می توان از روش ریزازدیادی استفاده نمود. هدف پروژه ریزازدی أکثر

آلوئه (Aloe littoralis) از نظر اقتصادی و دارویی اهمیت دارد. این گیاه بومی ایران تکثیر طبیعی و سنتی بسیار کند دارد. بنابراین، نمی تواند نیاز صنعت دارویی کشور را تامین کند. برای تکثیر سریع گیاه آلوئه (A.littoralis) می توان از روش ریزازدیادی استفاده نمود. هدف پروژه ریزازدیادی گیاه آلوئه بومی ایران (َA. littoralis) است. در این تحقیق ریزنمونه جوانه انتهایی و جوانه جانبی برروی محیط کشت پایهMS حاوی هورمون BAP با مقادیر (0/5, 1,2 میلی گرم بر لیتر) و هورمون IAA با مقادیر (0/5, 1 میلی گرم بر لیتر) کشت شدند. بیشترین میانگین تعداد ساقه (1.25) و بلندترین طول ساقه نوپدید (1.13 سانتی متر) در محیط کشت دارای BAP (2 mg/l) و IAA (0/5 mg/l) بوجود آمدند، همچنین بیشترین میانگین تعداد برگ (3.7) و تعداد ریشه (2.32) و بلندترین طول ریشه (4.66 سانتی متر) در محیط کشت دارای BAP (0/5 mg/l) و IAA (0/5 mg/l) تولید شدند، در ریزازدیادی گیاه آلوئه (A.littoralis) مصرف هورمون ها کم است، پایه بومی و در مناطق جنوب ایران قابل دسترس است، خواص دارویی مشابه با گیاه آلوئه ورا (Aloe barbedensis) دارد، در نتیجه هزینه تولید پایین است و از نظر اقتصادی مقرون به صرفه تر می باشد. تفاصيل المقالة

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3 - Micropropagation of Clematis orientalis L. culture in vitro
علی ایزدی صادق آبادی احمد خلیقی عبدالله قاسمی پیربلوطی مرضیه تقی پوردهکردی

Background & Aim: Clematis is an ornamental and medicinal plant that is primarily planted as anornamentalplant. Clematis orientalis is native to Asia and Central Europe, used as an antiseptic and refrigerant. The propagation of Clematis is very important. In this st أکثر

Background & Aim: Clematis is an ornamental and medicinal plant that is primarily planted as anornamentalplant. Clematis orientalis is native to Asia and Central Europe, used as an antiseptic and refrigerant. The propagation of Clematis is very important. In this study, the effects of plant growth regulators on rooting and growth of the plants in vitro culture were examined. Experimental: In this study, effects of growth regulators, including naphthalene acetic acid (NAA), at the concentrations of 0.0, 0.4, 0.5, and 0.6 mg/mL and Indol butyric acid (IBA) at the concentrations of 0.0, 0.4, 0.5, and 0.6 mg/mL on micropropagationin Murashige and Skoog’s medium (MS) were investigated. Results & Discussion: Results indicated that the effects growth regulators of auxin at different concentrations on in vitro growth micrpropagules were significant. The highest rooting and number of stems were obtained from control. Increase concentration of growth regulators decreased rooting. The highest decrease rate was obtained from0.6 mg/L auxin. Recommended applications/industries: The result of study indicated that positive effects lateral shoot explants and Murashige and Skoog’s medium (MS) without growth regulators on micropropagation. تفاصيل المقالة

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4 - In vitro propagation of orchid Phalaenopsis amabilis (L.) Blume var. Jawa
Mozhdeh Asa Behzad Kaviani

10.30495/ijpp.2020.672571

A protocol was developed for high frequency in vitro multiplication of an ornamental orchid, Phalaenopsis amabilis (L.) Blume var. Jawa, using plant growth regulators (PGRs). Protocorm-like bodies (PLBs), as explants were cultured on Murashige and Skoog (MS) medium fort أکثر

A protocol was developed for high frequency in vitro multiplication of an ornamental orchid, Phalaenopsis amabilis (L.) Blume var. Jawa, using plant growth regulators (PGRs). Protocorm-like bodies (PLBs), as explants were cultured on Murashige and Skoog (MS) medium fortified with various concentrations of kinetin (KIN) and indole-3-butyric acid (IBA), either individually or in combination. A combination of 1.00 mg l-1 KIN + 1.00 mg l-1 IBA was found to be suitable for regeneration of most measured characteristics especially maximum PLBs regeneration (30.40/plantlet), leaf number (5.93/plantlet), and root number (8.36/plantlet) from protocorm explants. The maximum number of plantlets (11.66) was calculated on MS medium supplemented with 1.00 mg l-1 KIN + 0.50 mg l-1 IBA, followed by 1.00 mg l-1 KIN + 1.00 mg l-1 IBA (10.33). Plantlets were transplanted to pots filled with cocochips and sphagnum moss (70:30) for acclimatization and transferred to the greenhouse. Upon ex vitro transfer, 90% of plants survived. تفاصيل المقالة

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5 - Influence of plant growth regulators (BA, TDZ, 2-iP and NAA) on micropropagation of Aglaonema widuri
Behazd Kaviani Shahram Sedaghathoor Mohammad Reza Safari Motlagh Seddigeh Rouhi

10.30495/ijpp.2019.668856

Aglaonema widuri is an evergreen and indoor ornamental plant. This study aimed to investigate the effect of some plant growth regulators on micropropagation of A. widuri. A protocol was developed for high frequency in vitro multiplication of A. widuri on the same medium أکثر

Aglaonema widuri is an evergreen and indoor ornamental plant. This study aimed to investigate the effect of some plant growth regulators on micropropagation of A. widuri. A protocol was developed for high frequency in vitro multiplication of A. widuri on the same medium for both shoots and roots induction. This condition decreases time duration and cost of micropropagation. Apical buds as explants were obtained from greenhouse-grown A. widuri and were established on Murashige and Skoog (MS) medium. Three cytokinins including N6-benzyladenine (BA; 0.00, 3.00, 3.50, and 4.00 mg l-1), 1-phenyl-3-(1,2,3-thiaiazol-5-yl), urea (TDZ; 0.00, 0.50, and 1.00 mg l-1), and N6-(2-isopentenyl) adenine (2-iP; 0.00 and 7.00 mg l-1), along with one auxin [α-naphtalene acetic acid (NAA; 0.00, 0.10, 0.20, 0.30, and 0.40 mg l-1) were studied for their effect on micropropagation of A. widuri. The plantlets were transferred to pots and grown in the greenhouse with a success rate of 95%. BA and NAA treatments as 3.00 mg l-1+ 0.2 mg l-1 recorded the highest shoot proliferation rate (number: 6.00 shoots and length: 7.75 cm per explant). Treatment of 4.00 mg l-1 BA + 0.10 mg l-1 NAA + 0.50 mg l-1 TDZ produced maximum nodes (13.25 per explant). The largest number of leaves (4.25 per explant) was produced in the medium containing 3.50 mg l-1 BA + 0.20 mg l-1 NAA. Maximum root initiation and development (14.25 per explant) was obtained on the medium containing 3.00 mg l-1 BA + 0.20 mg l-1 NAA. The combination of 3.50 mg l-1 BA + 0.20 mg l-1 NAA was found to be the most suitable growth regulator for obtaining the highest root length (8.25 cm per explant). تفاصيل المقالة

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6 - The effect of different hormone combinations on direct and indirect somatic embryogenesis in Agave americana
Maryam Nazir Shahab Sadat Mehdi Soltani Howyzeh

10.30495/ijpp.2019.664579

Direct and indirect somatic embryogenesis in Agave Americana through basal parts of micropropagated shoots were considered in this study. For micropropagation, 2 factors (BA and NAA) were utilized in different concentrations. A factorial experiment based on a completely أکثر

Direct and indirect somatic embryogenesis in Agave Americana through basal parts of micropropagated shoots were considered in this study. For micropropagation, 2 factors (BA and NAA) were utilized in different concentrations. A factorial experiment based on a completely randomized design (CRD) was accomplished with four replicates in a modified Murashige and Skoog medium (MSB). BA in 3 mg/L along with NAA in 0.1 mg/L was the best treatment for multiplication. Callus and embryo induction were evaluated using different auxins in a CRD experiment with three replicates in MS medium supplemented with L2 vitamins. Picloram 2.5 and 2 mg/L and also Dicamba 1 mg/L induced 66.7%, 60%, and 53.3% of explants to somatic embryo, respectively. Also, 2,4-D 2 mg/L had the highest impact on callus induction (95.6%). Embryogenesis was studied using Dicamba and Picloram in the same culture medium in a factorial experiment based on CRD with three replicates. The results of orthogonal polynomial analaysisindicated that high concentrations of Dicamba from 0 to 1.5 mg/L had a significant effect on somatic embryo induction. However, higher concentrations had the opposite effect on somatic embryo numbers. Germination of somatic embryos was performed in MSB medium without growth regulators. تفاصيل المقالة

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7 - Influence of plant growth regulators (BA, TDZ, 2-iP and NAA) on micropropagation of Aglaonema widuri
Behazd Kaviani Shahram Sedaghathoor Mohammad Reza Safari Motlagh Seddigeh Rouhi

10.30495/ijpp.2019.664575

Aglaonema widuri is an evergreen and indoor ornamental plant. This study aimed to investigate the effect of some plant growth regulators on micropropagation of A. widuri. A protocol was developed for high frequency in vitro multiplication of A. widuri on the same medium أکثر

Aglaonema widuri is an evergreen and indoor ornamental plant. This study aimed to investigate the effect of some plant growth regulators on micropropagation of A. widuri. A protocol was developed for high frequency in vitro multiplication of A. widuri on the same medium for both shoots and roots induction. This condition decreases time duration and cost of micropropagation. Apical buds as explants were obtained from greenhouse-grown A. widuri and were established on Murashige and Skoog (MS) medium. Three cytokinins [N6-benzyladenine (BA; 0.00, 3.00, 3.50, and 4.00 mgL-1), 1-phenyl-3-(1,2,3-thiaiazol-5-yl) urea (TDZ; 0.00, 0.50, and 1.00 mgL-1), and N6-(2-isopentenyl) adenine (2-iP; 0.00 and 7.00 mgL-1)] and one auxin [α-naphtaleneacetic acid (NAA; 0.00, 0.10, 0.20, 0.30, and 0.40 mgL-1)] were studied for their effect on miropropagation of A. widuri. BA and NAA treatments as 3.00 mgL-1 + 0.2 mgL-1 recorded the highest shoot proliferation rate (number: 6.00 shoots and length: 7.75 cm per explant). Treatment of 4.00 mgL-1 BA + 0.10 mgL-1 NAA + 0.50 mgL-1 TDZ produced maximum nodes (13.25 per explant). The largest number of leaf (4.25 per explant) was produced in the medium containing 3.50 mgL-1 BA + 0.20 mgL-1 NAA. Maximum root initiation and development (14.25 per explant) was obtained on medium containing 3.00 mgL-1 BA + 0.20 mgL-1 NAA. The combination of 3.50 mgL-1 BA + 0.20 mgL-1 NAA was found to be the most suitable growth regulators for obtaining the highest root length (8.25 cm per explant). The plantlets were transferred to pots and grown in the greenhouse with a success rate of 95%. تفاصيل المقالة

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8 - Callus Induction and Organogenesis Capacity from Lamina Explant of Petunia × hybrida F1 Induced by BA and NAA
Behzad Kaviani Danesh Kazemi

Plant growth regulators (PGRs) have an important role in callus induction and organogenesis of plant explants cultured in vitro conditions. Callus has a proper potential for plant regeneration through indirect organogenesis and embryogenesis as well suspension culture, أکثر

Plant growth regulators (PGRs) have an important role in callus induction and organogenesis of plant explants cultured in vitro conditions. Callus has a proper potential for plant regeneration through indirect organogenesis and embryogenesis as well suspension culture, genetic transformation and production of secondary metabolites. In current experiment, leaf explants of Petunia × hybrida F1 were cultured on basal Murashige and Skoog (MS) medium supplemented with different concentrations of N6-benzyladenine (BA) (0.25, 0.50 and 1.00 mg l−1) and ɑ-naphthalene acetic acid (NAA) (0.10, 0.20 and 0.30 mg l−1). The maximum fresh weight (5.16 g), dry weight (0.31 g) and volume of callus (24.50 cm3) was obtained in the medium containing 1.00 mg l−1 BA in combination with 0.30 mg l−1 NAA. Leaf explants did not produce callus in medium without PGRs. Plantlets were produced on all callus grown on the media containing PGRs. The most plantlets number was produced on callus induced on medium enriched with 1.00 mg l−1 BA in combination with 0.30 mg l−1 NAA. The plantlets regenerated in vitro with well-developed shoots and roots were successfully established in pots containing peat and perlite (1:1) and grown in a greenhouse within 4 weeks with a 100% survival rate. The regenerated plants were morphologically identical with mother plants and did not show any detectable phenotypic variation. تفاصيل المقالة

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9 - In vitro Propagation of a Cut Flower Variety Muscari armeniacum Leichtl. ex Bak. Through Direct Bulblet Proliferation Pathways
Md Omar Faruq Md Shahinozzaman Mustafa Azad Muhammad Amin

Muscari armeniacum is one of the important ornamental cut flower in floriculture industry which native to Southern Europe, North Africa, Western Asia and Asia Minor. In this study, bulb explants (basal plate of bulb having meristem), bulb scales and leaf segments from i أکثر

Muscari armeniacum is one of the important ornamental cut flower in floriculture industry which native to Southern Europe, North Africa, Western Asia and Asia Minor. In this study, bulb explants (basal plate of bulb having meristem), bulb scales and leaf segments from in vitro derived bulblets were culture in Murashige and Skoog (MS) medium with different plant growth regulator concentrations and combinations to assess growth regulators effect on different bulblet organogenesis pathways in vitro. The results demonstrated that cytokinin in combination with auxin is required for both axillary and adventitious bulblet regeneration. Benzyladenine (BA) - α-naphthalene acetic acid (NAA) combination, showed significant effects compared to other growth regulator combinations tested. 4.0 µM BA + 2.0 µM NAA was the found to the best for axillary bulblet formation from bulb explants. Likewise, bulb and leaf segments showed the best response in adventitious bulblet organogenesis when they were cultured in BA-NAA combinations. Out of several concentrations of BA with NAA, 4.0 µM BA + 1.0 µM NAA was optimum for adventitious bulblet regeneration. Bulblets, properly isolated from both axillary and adventitious proliferation systems, showed maximum percentage of rooting on half strength MS medium containing 2.0 µM Indole-3-butyric acid (IBA). However, the higher concentration of all the auxins showed either callus formation at the base of shoots or malformation of roots. All the in vitro regenerated plantlets were successfully acclimatized under ex vitro environment in the garden soil with 60% survival rate. تفاصيل المقالة

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10 - In Vitro Propagation of Cephalanthera rubra (L.) Rich., an Endangered Orchid, Using 2,4-D, NAA and BA
Mahdi Zargar Azad Behzad Kaviani Shahram Sedaghathoor

Orchids are universally popular due to the wide range of colors, sizes, shapes, and scents in their flowers. The demand for orchids as cut flowers and pot plants is increasing in the flower market. Seed germination of orchids is comparatively low in the wild as they typ أکثر

Orchids are universally popular due to the wide range of colors, sizes, shapes, and scents in their flowers. The demand for orchids as cut flowers and pot plants is increasing in the flower market. Seed germination of orchids is comparatively low in the wild as they typically require fungal symbionts during their germination. Micropropagation of orchids is a major approach to orchid conservation and commercial production. Cephalanthera rubra (L.) Rich., one of Iran’s endangered orchid species, has been cloned using leaf as explant, Murashige and Skoog (MS) as culture medium, and α-naphthalene acetic acid (NAA) and 2,4-dichlorophenoxyacetic acid (2,4-D) (both at the concentrations of 0, 0.1, 0.2, 0.3 and 0.4 mg L–1) as well 6-benzyladenine (BA) (at 0, 1, 2, 3 and 4 mg L–1) as plant growth regulators (PGRs). In order to initiate an axenic culture, the disinfection of leaf explants was performed with sodium hypochlorite and mercury chloride. The highest number of shoots (4.33) was obtained in medium enriched with 4 mg L–1 BA. Maximum stem length (4.73 cm), leaf number (5.33) and node number (2.86) was obtained in medium supplemented with 3 mg L–1 BA. The largest number of root (5) and the highest length of root (4.83 cm) was produced on medium augmented with 0.3 mg L–1 NAA. Rooted plantlets were transferred to pots filled with peat and perlite in 1:1 proportion and acclimatized to ambient greenhouse conditions with an average of 90% survival rate. This is the first report on the micropropagation of C. rubra (L.) Rich. تفاصيل المقالة

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11 - The Effect of Plant Growth Regulators on Callus Induction and Regeneration in Three Chrysanthemum (Chrysanthemum grandiflorum Ramat) Cultivars Under In Vitro Culture Condition
Mohammad Reza Shafiei Abdollah Hatamzadeh Pejman Azadi Habibollah Samizadeh Lahiji

Chrysanthemums are known as one of the top three flowers in the floriculture industry. This ornamental plant is traditionally propagated by cuttings. The use of in vitro subculture is expanding for plants like chrysanthemums that are propagated by asexual methods. This أکثر

Chrysanthemums are known as one of the top three flowers in the floriculture industry. This ornamental plant is traditionally propagated by cuttings. The use of in vitro subculture is expanding for plants like chrysanthemums that are propagated by asexual methods. This research evaluated the effect of plant growth regulators on callus induction and regeneration in three chrysanthemum cultivars including ‘Bonfire Yellow’, ‘Rambla’, and ‘Bella Rosa’. For callus generation, the leaf explants were cultivated in the Murashige and Skoog (MS) culture medium modified with with benzyl adenine (1, 2, or 3 mg/L BA) and naphthaleneacetic acid (0.5 or 1 mg/L NAA) in a factorial experiment based on a completely randomized design. The highest rate of regeneration of ‘Bonfire Yellow’(31.25%), ‘Rambla’(25%), and ‘Bella Rosa’ (31.25%) were obtained from the culture media containing 2 mg/L BA + 1 mg/L NAA, 1 mg/L BA + 1 mg/L NAA, and 3 mg/L BA + 0.5 mg/L NAA, respectively. تفاصيل المقالة

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12 - افزایش جوانه‌زنی Habenaria janellehayneana (Orchidaceae): بررسی روش‌های غیرهمزیستی و همزیستی
ثیرا توماوونگسا چاتاپوند موزیموندو سانتی واتانا استفان گیل راتاکت چویکلین نطاووت ویریاتاناودی وونگ نودوان موانگسان

Habenaria janellehayneana Choltco, Moloney, & Yong Gee (Orchidaceae) is a lithophytic orchid with striking pink flowers that is endemic to Phitsanulok Province, northern Thailand. Only a few populations of this species are found in Phu Hin Rong Kla National Park. To أکثر

Habenaria janellehayneana Choltco, Moloney, & Yong Gee (Orchidaceae) is a lithophytic orchid with striking pink flowers that is endemic to Phitsanulok Province, northern Thailand. Only a few populations of this species are found in Phu Hin Rong Kla National Park. To maintain rare plant species in ex situ collections thereby preventing extinctions, along with the aim of mass propagation for ornamental reasons, it is crucial that suitable propagation methods are developed. In this paper, we describe protocols for the asymbiotic and symbiotic germination of H. janellehayneiana. Of the four growing media tested, germination percentages were greatest on ½ VW (18.97%), followed by ½ MS (14.20%), MS (12.46%), and VW (11.93%) at 16 weeks, and protocorm development was most advanced (stage 4) within 10 weeks. Of the three plant growth regulators tested, including 6-benzylaminopurine (BAP), gibberellic acid (GA), and thidiazuron (TDZ), at 0, 1, 3, and 5 mg/L concentrations, 1 mg/L BAP significantly enhanced seed germination (p <0.05) when compared to the control (8.47%). For symbiotic seed germination, two non-mycorrhizal endophytic fungi isolates of the genera Aspergillus and Colletotrichum increased seed germination by 14.03% and 11.00% respectively, when compared to the control (6.15%). These findings demonstrate that it is possible to germinate the seeds of H. janellehayneana via both asymbiotic and symbiotic method, with a symbiotic approach providing the best outcomes, and this could assist in the conservation of this and other rare terrestrial orchids, as well as increase their value in the ornamental market. تفاصيل المقالة

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13 - Somatic Embryogenesis and Plant Regeneration from Embryonic Axes and Cotyledons Explants of Tea (Camellia sinenesis L.)
B. Kaviani

In the present study, 2, 4-dichlorophenoxyacetic acid (2, 4-D) was assessed individually for its effectiveness to induce somatic embryogenesis in tea (Camellia sinenesis L.). Embryonic axes and cotyledons explants were dissected from the seeds. Explants were cultured on أکثر

In the present study, 2, 4-dichlorophenoxyacetic acid (2, 4-D) was assessed individually for its effectiveness to induce somatic embryogenesis in tea (Camellia sinenesis L.). Embryonic axes and cotyledons explants were dissected from the seeds. Explants were cultured on Murashige and Skoog (MS) medium containing 0, 1 and 5 mM 2, 4-D alone for embryonic axes and 0, 1 and 5 mM 2, 4-D along with 0 and 0.5 mM IBA for cotyledons. Embryos were observed in embryonic axes explants cultured on MS medium containing 1mM 2, 4-D. No somatic embryos were seen on cotyledons explants. تفاصيل المقالة

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14 - Micropropagation of Rosa canina Through Axillary Shoot Proliferation
Mahboubeh Davoudi Pahnekolayi Ali Tehranifar Leila Samiei Mahmoud Shoor

In vitro propagation of rose has played a very important role in rapid multiplication of species with desirable traits and production of healthy and disease-free plants. Micropropagation using nodal segments of Rosa canina under different combinations of BAP, GA3 and NA أکثر

In vitro propagation of rose has played a very important role in rapid multiplication of species with desirable traits and production of healthy and disease-free plants. Micropropagation using nodal segments of Rosa canina under different combinations of BAP, GA3 and NAA on MS and VS, medium was investigated. The results showed that the highest shoot proliferation was obtained on VS medium containing 2 mg/L BAP without any GA3 and NAA. Furthermore the highest root regeneration obtained in half strength VS medium. The present investigation recommended a practiciable in vitro plant protocol for R. canina as an important step for successful implementation of biotechnological techniques for rose improvement in Iran. تفاصيل المقالة

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15 - Direct Multiple Shoot Regeneration from Shoot Tip and Nodal Explants of Solanum Nigrum L. A Medicinal Herb
M.S. Kavitha E.G. Wesely P. Mehalingam

In vitro multiple shoot regeneration of Solanum nigrum L., an Indian medicinal plant was accomplished on MS medium utilizing shoot tip and nodal explants. Direct multiple shoots differentiated within 6 weeks when explants were cultured on MS medium containing BAP (1.0-5 أکثر

In vitro multiple shoot regeneration of Solanum nigrum L., an Indian medicinal plant was accomplished on MS medium utilizing shoot tip and nodal explants. Direct multiple shoots differentiated within 6 weeks when explants were cultured on MS medium containing BAP (1.0-5.0 mg/l) and KIN (1.0-5.0 mg/l) individually. Among various concentrations of cytokinins tested, maximum number of multiple shoots was obtained on MS medium supplemented with BAP (1.0 mg/l) from shoot tip (20.4±0.22) and MS medium supplemented with BAP (3.0 mg/l) from nodal explants (8.4±0.22). The in vitro regenerated shoots were rooted (8.4±0.16 roots per shoot) on MS medium supplemented with NAA (1.0 mg/l) within 2-3 weeks of culture and the regenerated plantlets could be successfully established in soil where they grow normally. تفاصيل المقالة

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16 - An Efficient and Cost Effective Protocol for In Vitro Propagation of Pineapple
Ipsita Dutta Joyita Bhadra Pritha Ghosh Babita Saha Siraj Datta

An efficient and cost effective protocol for in vitro propagation of Pineapple (Ananas comosus var. Queen) has been developed. In the proliferation stage, agar based Murashige and Skoog (MS) media was supplemented with 3.0 mg/l benzyleaminopurine (BAP), 0.5 mg/l indole أکثر

An efficient and cost effective protocol for in vitro propagation of Pineapple (Ananas comosus var. Queen) has been developed. In the proliferation stage, agar based Murashige and Skoog (MS) media was supplemented with 3.0 mg/l benzyleaminopurine (BAP), 0.5 mg/l indole acetic acid (IAA) and 50 mg/l adenine sulphate as RBC design experiment. Two approaches were taken to reduce the chemical cost of micropropagation media. Analytical grade sucrose was successfully replaced by commercial sugar, completely during proliferation stage and up to 66% during rooting stage. Again during the rooting stage, agar based solid media was replaced by liquid media (MSmedia). Bio-degradable Coir and Luffa were used as supporting matrix. As supporting matrix in rooting media, Luffa was found to be more effective. The clonal fidelity of in vitro raised plantlets was confirmed by RAPD technique. تفاصيل المقالة

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17 - Optimization of the Sterilization and Establishment Steps for Almonds 2-22 Genotype
Fatemeh Alizadeh Arimi Abbas Yadollahi Ali Imani Mohammad Fakoor-Aryan

10.22034/jon.2020.1908563.1092

The almond is one of the most important nut crops in many countries, including Iran. On the other hand, due to the difficult rooting in conventional propagation methods such as cuttings, its propagation has faced challenges. Therefore, this study was aimed at optimizing أکثر

The almond is one of the most important nut crops in many countries, including Iran. On the other hand, due to the difficult rooting in conventional propagation methods such as cuttings, its propagation has faced challenges. Therefore, this study was aimed at optimizing in vitro culture conditions for the promising 2-22 genotype as a high-yield and late-blooming cultivar. To this end, after collecting apical and lateral buds of 2-22 genotype, surface sterilization and establishment treatments were applied to explants. The second-order equation is selected for the central compound design (CCD) with two variables (HgCl2 and NaClO) to obtain a good fit in the sterilization treatment. The results showed that 1.8 % NaClO for 12 min + 0.1 % HgCl2 for 3 min and 1.5 % NaClO for 8 min with 81.25%, and 100% of healthy seedlings were the best treatment in contamination control and explant Viability, respectively. The experimental establishment was conducted as a factorial experiment using a completely randomized design (CRD) with four replications. The establishment treatments indicated that the WPM medium was more effective than the MS medium and Knop medium. The hormonal composition of 1 mgL-1 BA + 0.05 mgL-1 IBA had the best results in the percentage of the establishment (72.25%), number of foliage (7.24), and shoot length (13.77 mm). تفاصيل المقالة

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18 - Enhancing Growth and Morpho-physiological Traits of Tissue-cultured Explants of Persian Walnut through Manipulation of In vitro Lighting Spectra
Seyyed Arash Saeedi Kourosh Vahdati Sasan Aliniaeifard Saadat Sarikhani Shirin Dianati Maryam Davarzani Safieh Fakhari

10.22034/jon.2024.2004910.1259

There are numerous challenges associated with the large-scale production of walnut In vitro-plantlets. It is imperative to develop new environmental control systems for its In vitro propagation. Additionally, there is a lack of knowledge regarding the impacts of lightin أکثر

There are numerous challenges associated with the large-scale production of walnut In vitro-plantlets. It is imperative to develop new environmental control systems for its In vitro propagation. Additionally, there is a lack of knowledge regarding the impacts of lighting systems on the morpho-physiological traits and biomass accumulation in walnut tissue-cultured explants. In this study, walnut nodal shoots were subjected to eight different light spectra, including white, blue, red, green, far-red, blue-red combination, red-far-red combination, and a fluorescent lamp serving as a control, over a period of 28 days. The results indicated that combined spectra treatments, such as blue-red and red-far-red, led to improved biomass accumulation (total fresh and dry weight) compared to other monochromatic light spectral treatments. Furthermore, Light-emitting diode (LED) treatments had a discernible impact on the morpho-physiological traits of walnut In vitro-explants. Specifically, white light spectra enhanced Specific leaf area (SLA), while the green light spectra increased leaf water content (LWC) when compared to other light treatments. Additionally, the application of far-red light elevated leaf mass area (LMA) and water content per unit leaf area (LWCA). The findings of this study demonstrate that the quality, morphological, and growth characteristics of In vitro explants of walnut can be enhanced by utilizing specific light spectra. تفاصيل المقالة

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