فهرس المقالات MTNR1A

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  1 - Association between <i>MTNR1A</i> and <i>CYP19</i> Genes Polymorphisms and Economic Traits in Kurdi Sheep
  ز. داوری ورانلو س. حسنی م. آهنی آذری ف. صمدی س. زکی‌زاده ع.ر. خان احمدی
  The ovine melatonin receptor 1A (MTNR1A) and aromatase (CYP19) genes were structurally characterized and the association between their variants and reproductive and growth traits was studied in Kurdi sheep at Kurdi sheep breeding station located in Shirvan, Iran. The ge أکثر

  The ovine melatonin receptor 1A (MTNR1A) and aromatase (CYP19) genes were structurally characterized and the association between their variants and reproductive and growth traits was studied in Kurdi sheep at Kurdi sheep breeding station located in Shirvan, Iran. The genomic DNA was extracted by guanidine thiocyanate-silica gel method. Polymerase chain reaction was carried out to amplify 824 bp fragment of exon 2 of MTNR1A and 140 bp fragment of the exon 3 of the ovine CYP19 genes. The PCR products were digested with restriction endonucleases RsaI for MTNR1A and BstMBI for CYP19 genes and checked by polyacrylamide gel electrophoresis for the presence of restriction sites. Two alleles were found for all the loci investigated, which were named as A and B for CYP19, and R and r for MTRN1A. Allelic frequencies for MTRN1A were 0.49 and 0.51 for R and r alleles, while in the case of CYP19 gene, frequencies were 0.475 and 0.525for A and B alleles, respectively. Association analysis did not show any significant relations between MTNR1A gene polymorphisms and litter size (LS), age at first lambing (AFL) and lambing interval (LI). Moreover, CYP19 gene polymorphism did not affect birth weight (BW), weaning weight (WW), 6, 9 and 12 months (YW) body weights, age at first lambing (AFL) and lambing interval (LI). تفاصيل المقالة
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  2 - Association of Melatonin Receptor 1A Gene Polymorphisms with Production and Reproduction Traits in Zandi Sheep
  م. حاتمی ق. رحیمی میانجی ا. فرهادی
  Melatonin regulates some major physiological processes such as maturation and function of reproductive system, pubertal development, seasonal reproduction and adaptation. The activation of melatonin hormone is mediated by melatonin receptor. Previous studies showed mela أکثر

  Melatonin regulates some major physiological processes such as maturation and function of reproductive system, pubertal development, seasonal reproduction and adaptation. The activation of melatonin hormone is mediated by melatonin receptor. Previous studies showed melatonin receptor 1A gene (MTNR1A) is highly polymorphic in seasonally breeding species. The aim of the present study was detection of MTNR1Apolymorphism and its association with production (body weights at birth, 1, 3, 6, 9 and 12 month of age) and reproduction (litter size) traits in Zandi sheep using PCR-RFLP methodology. Hundred blood samples were randomly collected and genomic DNA was isolated using modified salting out method. A large fragment of exon 2 of MTNR1A gene was amplified by PCR using specific primer pairs. After amplification, the PCR product was digested with MnII endonuclease. Restricted digestion allowed the determination of two alleles (M, m) and two genotypes (MM, Mm) with frequencies of 0.91, 0.09 and 0.82, 0.18, respectively. Least square means showed that MM individuals had higher body weight at one month of age (BW1) than Mm individuals (P&lt;0.05). No associations were found between observed genotypes and other studied traits.In the subsequent studies using a large number of samples along with the other important parameters such as sire effect and the lamb health status which may influence lamb body weight is recommended. تفاصيل المقالة
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  3 - Association between Melatonin Receptor 1A (<i>MTNR1A</i>) Gene Polymorphism at the <i>MnlI</i> Site and Production Traits in Shal and Crossbreeding between Shal and Romanov
  ا. افروزنیا م. زندی م.ر. سنجابی م.ح. هادی تواتری
  The objective of this study was to evaluate the relationship between polymorphism at the MnlI site of MTNR1A gene and production traits of Shal and Romanov crossbreeding. The crossbreeding between Shal and Romanov was done on 600 Shal ewes. For this reason, Shal ewes we أکثر

  The objective of this study was to evaluate the relationship between polymorphism at the MnlI site of MTNR1A gene and production traits of Shal and Romanov crossbreeding. The crossbreeding between Shal and Romanov was done on 600 Shal ewes. For this reason, Shal ewes were laparoscopically and artificially inseminated withthe semen of Romanov breed. For the preparation of genomic DNA, a total of 90 ewes, either Shal (n=50) or Shal &times; Romanov (n=40), were used and an 824 bp fragment of exon 2 of the MTNR1A gene was amplified. In this research, two genotypes MM and Mm were detected in the study Shal population with frequencies of 0.78 and 0.22, respectively, while MM and mm genotypes of the study crossbreeds were detected with 0.125 and 0.0875 frequencies, respectively. However, the mm and Mm genotypes were not detected in Shal and Shal &times; Romanov crossbreeds, respectively. The MnlI marker site in Shal &times; Romanov breeds had a significant effect (P&gt;0.05) on body weight after 3 months (BW3) as the ewes with MM genotypes had significantly higher BW3 than mm individuals. However, no significant difference was observed between MM and mm marker sites for birth weight (BW) and 6-month body weight (BW6). The same results were observed for Shal breeds as when MM and Mm genotypes were detected for BW, BW3 and BW6 traits. In conclusion, in this study, mm genotype as a measurement for litter size in sheep breeds was successfully introduced into Shal &times; Romanov crossbreeds, while their production traits were not affected by MnlI genotypes. تفاصيل المقالة