Evaluating the Potential of Polycaprolactone/Heparinized Nano Fluoro Hydroxyapatite Composite Scaffolds for Advancing Bone Tissue Engineering: A Comprehensive Analysis of Biodegradability and Water Absorption
الموضوعات :Nila Haghani 1 , Nahid Hasanzadeh Nemati 2 , Mohammad Taghi Khorasani 3 , Shahin Bonakdar 4
1 - Department of Biomedical Engineering, Science and Research Branch, Islamic Azad University, Tehran, Iran
2 - Department of Biomedical Engineering, Science and Research Branch, Islamic Azad University, Tehran, Iran
3 - Biomaterial Department of Iran Polymer and Petrochemical Institute, Tehran, Iran
4 - National Cell Bank Department, Pasteur Institute of Iran, Tehran, Iran
الکلمات المفتاحية: Scaffold, Water Absorption, Heparinized Nano Fluoro Hydroxyapatite, Regenerative Medicine,
ملخص المقالة :
The primary purpose of this study is to produce a composite scaffold using polycaprolactone (PCL) and heparinized nano-fluorohydroxyapatite for cancellous bone tissue engineering. The research investigated the impact of heparinized nano-fluorohydroxyapatite particles on various properties of the scaffold, including water absorption, biodegradability, and alkaline phosphatase activity. The scaffold was produced using the phase separation (solid-liquid) method in combination with freeze-drying, and two different concentrations of heparinized nano-fluorohydroxyapatite powder were utilized. Biodegradability was assessed by submerging the scaffolds in phosphate-buffered saline for 6 weeks, showing that increased nano-fluorohydroxyapatite content enhanced biodegradability. PCL/10%w(H-nFHA50) exhibited the highest biodegradability rate. Water absorption analysis revealed that PCL scaffolds had lower water absorption compared to composite samples with heparinized nano-fluorohydroxyapatite, with PCL/10%(H-nFHA50) demonstrating the highest water absorption. Alkaline phosphatase activity was assessed on day 14 of cell culture, showing higher activity in heparinized samples compared to heparin-free samples in the presence of nano-fluorohydroxyapatite. The presence of heparin and nano-fluorohydroxyapatite in the scaffold structure likely contributed to this difference. No significant difference was observed between heparinized scaffolds with different nano-fluorohydroxyapatite concentrations. The results emphasize that the constructed scaffolds possess the potential for utilization in cancellous bone tissue engineering.
[1] Mallick, S., Tripathi, S. and Srivastava, P. 2015. Advancement in scaffolds for bone tissue engineering: A Review. Journal of Pharmacy and Biological Sciences. 10(1):37-54. doi: 10.9790/3008-10143754.
[2] Fergal, J. and Brien, O. 2011. Biomaterials & scaffolds for tissue engineering. Materials Today. 14(3):88-95. doi: 10.1016/S1369-7021(11)70058-X.
[3] Rezaei, M., Hassanzadeh Nemati, N., Mehrabani, N. and Komeili, A. 2022. Characterization of sodium carboxymethyl cellulose/calcium alginate scaffold loaded with curcumin in skin tissue engineering. Journal of Applied Polymer Science. 139(22):52271. doi:10.1002/app.52271.
[4] Hassanzadeh Nemati, N. and Mirhadi, S. M. 2020. Synthesis and characterization of highly porous tio2 scaffolds for bone defects. International Journal of Engineering. 33(1): 134–140. doi: 10.5829/ije.2020.33.01a.15.
[5] Zadpoor, A. 2015. Bone tissue regeneration: The role of scaffold geometry. Biomaterial Sciences. 3:231–245. doi: 10.1039/C4BM00291A.
[6] Antoni, D., Burckel, H., Josset, E. and Noel, G. 2015. Three-dimensional cell culture: A breakthrough in vivo. International Journal of Molecular Sciences. 16:5517–5527. doi: 10.3390/ijms16035517.
[7] Boccaccini, A. R. and Blaker, J. J. 2005. Bioactive composite materials for tissue engineering scaffolds. Expert Review of Medical Devices. 2:303–317. doi: 10.1586/17434440.2.3.303.
[8] Dhandayuthapani, B., Yoshida, Y., Maekawa, T. and Kumar, D. S. 2011. Polymeric scaffolds in tissue engineering application: A Review. International Journal of Polymer Science. Article ID 290602. doi: 10.1155/2011/290602.
[9] He, C., Jin, X. and Ma, P. X. 2014. Calcium phosphate deposition rate, structure and osteoconductivity on electrospun poly (l-lactic acid) matrix using electrodeposition or simulated body fluid incubation. Acta Biomaterialia. 10:419–427. doi:10.1016/j.actbio.2013.08.041.
[10] Tony, Y., Sujee, J. and Naleway S. E. 2021. Characterization of porous fluoro hydroxyapatite bone-scaffolds fabricated using freeze casting. Journal of the Mechanical Behavior of Biomedical Materials. 123:104717. doi:10.1016/j.jmbbm.2021.104717.
[11] Asefnejad, A., Behnamghader, B. and Khorasani, M. T. 2011. Polyurethane/fluor-hydroxyapatite nanocomposite scaffolds for bone tissue engineering. International Journal of Nanomedicine. 6:93-100. doi: 10.2147/IJN.S13385.
[12] Deepthi, S. 2016. An overview of chitin or chitosan/nano ceramic composite scaffolds for bone tissue engineering. International. Journal of Biology and Macromolcules. 93:1338–1353. doi: 10.1016/j.ijbiomac.2016.03.041.
[13] Lauren, S., Selcuk, G., Xuejun, W., Milind, G. and Wei, S. 2007. Fabrication of three dimensional polycaprolactone/hydroxyapatite tissue scaffolds and osteoblast-scaffold interactions in vitro. Biomaterials. 28:5291–5297. doi:10.1016/j.biomaterials.2007.08.018.
[14] Hutmacher, D. W., Schantz, T., Zein, I., Ng, K. W., Teoh, S. H. and Tan, K. C. 2001. Mechanical properties and cell cultural response of polycaprolactone scaffolds designed and fabricated via fused deposition modeling. Journal of Biomedical Materials Research. 55:203–216. doi: 10.1002/1097-4636(200105)55:2<203::aid-jbm1007>3.0.co;2-7.
[15] Mohamed, R. M. and Yusoh, K. 2015. A review on the recent research of polycaprolactone (PCL). Advanced Materials Research. 1134:249–255. doi:10.4028/www.scientific.net/AMR.1134.249.
[16] König, U., Lode, A. et. al. 2013. Heparinization of a biomimetic bone matrix: integration of heparin during matrix synthesis versus adsorptive post surface modification. Journal of Materials Science. Materials in Medicine. 25:607-621. doi: 10.1007/s10856-013-5098-8.
[17] Gümüşderelioğlu, M. and Aday, S. 2011. Heparin-functionalized chitosan scaffolds for bone tissue engineering. Carbohydrate Research. 346(5):606-613. doi:10.1016/j.carres.2010.12.007.
[18] Teixeira, S., Yang, L., Dijkstra, P. J., Ferraz, M. P. and Monteiro, F. J. 2010. Heparinized hydroxyapatite/collagen three-dimensional scaffolds for tissue engineering. Journal of Materials Science: Materials in Medicine. 21(8):2385-92. doi: 10.1007/s10856-010-4097-2.
[19] Zhao, D. M., Wang, Y. X., Chen, Z. Y., Xu, R. W., Wu, G. and Yu, D. S. 2008. Preparation and characterization of modified hydroxyapatite particles by heparin. Biomedical Materials. 3(2):025016. doi: 10.1088/1748-6041/3/2/025016.
[20] Haghani, N., Hassanzadeh Nemati, N., Khorasani, M. T. and Bonakdar, SH. 2023. Fabrication of polycaprolactone/heparinized nano fluorohydroxyapatite scaffold for bone tissue engineering uses. International Journal of Polymeric Materials and Polymeric Biomaterials. 16:1-12. doi:10.1080/00914037.2023.2182781.
[21] Xiao, H., Huang, W., Xiong, K., Ruan, S. et. al. 2019. Chitosan, and nano-hydroxyapatite. International Journal of Nanomedicine. 22(14):2011-2027. doi: 10.2147/IJN.S191627.
[22] Venkatesan, J., Pangestuti, R., Qian, Z. J., Ryu, B. and Kim, S. K. 2010. Biocompatibility and alkaline phosphatase activity of phosphorylated chitooligosaccharides on the osteosarcoma MG63 cell line. Functional Biomaterials. 1:3-13. doi: 10.3390/jfb1010003.
[23] Woodruff, M. and Hutmacher, D. 2010. The return of a forgotten polymer -polycaprolactone in the 21st century. Progress in Polymer Science. 35(10):1217-1256. doi:10.1016/j.progpolymsci.2010.04.002.
[24] Christopher, X. F. L. 2008. Dynamics of in vitro polymer degradation of polycaprolactone-based scaffolds: accelerated versus simulated physiological conditions. Biomedical Materials. 3(3):034108-034108. doi: 10.1088/1748-6041/3/3/034108.