In Vitro Behavior of a Novel Nanostructure Hardystonite/Biphasic Calcium Phosphate Scaffolds (HT/BCPS); Evaluation and Investigation
hassan gheisari
1
(
najafabad university,isfahan,iran
)
الکلمات المفتاحية: in vitro, Nanostructure, Calcium Phosphate, Hardystonite, TEM&SEM,
ملخص المقالة :
An important compete in bone tissue engineering is the development of constructs serving as scaffolds to fill bone defects, and promote bone regeneration. In this study, highly porous almost (75%) nanostructured Hardystonite/biphasic calcium phosphate scaffolds (BCPS) with interconnected porosity was developed using various hardystonite (HT) contents via space holder technique. Transmission electron microscopy (TEM), X ray diffraction (XRD) and scanning electron microscopy (SEM) techniques was employed to evaluate different samples. In addition to, the agents of scaffold composition on mechanical behavior , bioactivity and biodegradability was studied. Also, the results showed that the produced scaffolds had an average pore size and density between 250-350 µm and 2.2 ± 0.4 - 1.7 ± 0.2 gr/cm3, respectively, depending on the Hardystonite (HT) with different contents. Furthermore, increasing the hardystonite content of scaffolds from 0 (control) to 30 wt. % enhanced the bioactivity test, biodegradability, and compressive strength from 1.1 ± 0.1 to 3.1 ± 0.2 MPa, respectively. Besides, MTT assay also confirmed that the BCPS30 (containing 30 wt. % of hardystonite) significantly promoted cell viability and cell adhesion compared to BCPS 0. Totally, our project suggests that nanostructured hardystonite/BCPS with improved biological and mechanical behavior (properties) could potentially be used for biomedical engineering such as bone tissue engineering application.
