بررسی اثر نانو ذرات کیتوزان و کیتوزان بر برخی صفات فیزیولوژیک و فیتوشیمیایی گیاه دارویی سیاه دانه Nigella sativa L.
الموضوعات :
فرحناز مهدی پور
1
,
سارا سعادتمند
2
,
علیرضا ایرانبخش
3
,
بهاره نوروزی
4
,
زهرا اوراقی اردبیلی
5
1 - دانشجوی دکتری، گروه زیست‌شناسی، دانشکده علوم پایه، واحد علوم وتحقیقات، دانشگاه آزاداسلامی، تهران، ایران
2 - دانشیار، گروه زیست‌شناسی، دانشکده علوم پایه، واحد علوم وتحقیقات، دانشگاه آزاداسلامی، تهران، ایران
3 - استاد، گروه زیست‌شناسی، دانشکده علوم پایه، واحد علوم وتحقیقات، دانشگاه آزاداسلامی، تهران، ایران
4 - استادیار، گروه زیست‌شناسی، دانشکده علوم پایه، واحد علوم وتحقیقات، دانشگاه آزاداسلامی، تهران، ایران
5 - دانشیار، گروه زیست‌شناسی، دانشکده علوم پایه، واحد گرمسار، دانشگاه آزاداسلامی،گرمسار، ایران
الکلمات المفتاحية: آنتی‌اکسیدان, پراکسیداسیون لیپید, سیاه دانه (Nigella sativa L.), نانو ذرات کیتوزان,
ملخص المقالة :
سیاه دانه (Nigella sativa L.) ازخانواده آلاله،یکی از بهترین منابع آنتی‌اکسیدان‌های طبیعی به شمار می‌رود. با توجه به تاثیر مثبت کیتوزان بر گیاهان دارویی گوناگون در این مطالعه به بررسی عملکرد رویشی و شیمیایی این گیاه تحت تیمار نانوذرات کیتوزان پرداختیم. فاکتورهای آزمایش شامل محلول دهی کیتوزان ونانوذرات آن با غلظت‌های 0.01، 0.05، 0.2، 1، 4 (pH 5) درصد بودند.سنجش‌ها در سال 1400 در آزمایشگاه رازی دانشگاه آزاد، واحد علوم و تحقیقات تهران بر روی عصاره دانه و برگ گیاه تیمارشده انجام گردید. عصاره‌گیری به روش پرس سرد انجام شد.برخی از صفات نظیر جوانه‌زنی (تعداد، درصد، شاخص و ضریب سرعت جوانه‌زنی)، پارامترهای رشد (طول ریشه‌چه و ساقه چه،وزن تر ریشه چه وساقه چه و وزن خشک ریشه چه و ساقه چه)،رنگیزه ها،میزان فنل کل برگ (فولین-سیوکالتو)، فلاونوئید کل برگ (رنگ سنجی آلومینیوم کلرید)، فعالیت آنتی‌اکسیدانی برگ (DPPH)، پراکسیداسیون لیپیدهای غشاء برگ (سنجش غلظت MDA) ومیزان پروتئین محلول دانه و برگ (بردفورد) ارزیابی شدند. آزمایش به صورت طرح کاملاً تصادفی با 3 تکرار و مقایسه میانگین داده‌ها با استفاده آزمون دانکن در سطح آماری5 درصد انجام شد.نتایج نشان داد درصدهای تیماری مورد نظر بر همه صفات مورد ارزیابی(جز وزن تر ریشه چه)تاثیر معنی داری دارند. تیمار 1 تا 0.01 درصد نانو ذرات کیتوزان سبب افزایش پارامترهای رشد و جوانه زنی شد.همچنین میزان فنل،فلاونوئید و فعالیت آنتی‌اکسیدانی در مقایسه با شاهد افزایش نشان داد که بیشترین مقدار افزایش در غلظت‌های 1 و 0.01 درصد نانوذرات کیتوزان مشاهده شد. حداکثر افزایش میزان رنگدانه‌ها تحت تاثیر غلظت1 و0.2 درصد نانوذرات کیتوزان بود.هر دو تیمار در غلظت 1 درصد سبب کاهش مقدار MDA نسبت به کنترل شدند. مقدار پروتئین کل در برگ و دانه تحت تاثیر تیمارها کاهش یافت .به طورکلی نتیجه شد که تیمار نانو ذرات کیتوزان به عنوان نوعی محرک زیستی بر بهبود ویژگی‌های کیفی سیاه دانه تاثیر مثبتی داشته و نانوذرات کیتوزان به عنوان محرکی مناسب جهت افزایش رشد پیشنهاد می‌شود.
المصادر:
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Balyan, P., Shinde, S., & Ali, A. 2021. Potential activities of nanoparticles synthesized from Nigella sativa L. and its phytoconstituents: An overview. Journal of Phytonanotechnology and Pharmaceutical Sciences, 1(2): 1-9.
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Ahmad, M. F., Ahmad, F. A., Ashraf, S. A., Saad, H.H., Wahab, S., Khan, M.I., Ali, M., Mohan, S., Hakeem, K.R., & Athar, M. T. 2021. An updated knowledge of Black seed (Nigella sativa): Review of phytochemical constituents and pharmacological properties. Journal of herbal medicine, 25, 100404. https://doi.org/ 10.1016/j.hermed.2020.100404
Zehtab-Salmasi, S., Javanshir, A., Omidbaigi, R., Alyari, H., & Ghassemi-Golezani, K. 2001. Effects of water supply and sowing date on performance and essential oil production of anise (pimpinella anisum l.). Acta Agronomica Hungarica, 49: 75-81. DOI:17557/tjfc.92800
Amer, A.H., & Shoala, T. 2020. Physiological and phenotypic characters of sweet marjoram in response to pre-harvest application of hydrogen peroxide or chitosan nanoparticles. Scientia Horticulturae, 268, 109374. DOI:1016/j.scienta.2020.109374
Amiri, A., Ismailzadeh Mahabadi, p., Sirus Mehr, A.R. 2014. The effect of chitosan foliar application on Yield and yield components of safflower in arteries (drought stress). Paper presented at the National Conference on Engineering and Management Agriculture. Sustainable environment and natural resources, Hamadan.iran.
Balyan, P., Shinde, S., & Ali, A. 2021. Potential activities of nanoparticles synthesized from Nigella sativa L. and its phytoconstituents: An overview. Journal of Phytonanotechnology and Pharmaceutical Sciences, 1(2): 1-9.
Botnick, I. X. 2012. Distribution of primary and specialized metabolites in Nigella sativa seeds, a spice with vast traditional and historical uses. Molecules, 17(9): 10159-10177.
Braca, A., Sortino, C., Politi, M., Morelli, I., & Mendez, J. 2002. Antioxidant activity of flavonoids from Licania licaniaeflora. Journal of ethnopharmacology, 79(3): 379-381.
Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical biochemistry, 72(1-2): 248-254.
Brand-Williams, W., Cuvelier, M. E., & Berset, C. L. W. T. 1995. Use of a free radical method to evaluate antioxidant activity. LWT-Food science and Technology, 28(1): 25-30.
Chandra, S., Chakraborty, N., Dasgupta, A., Sarkar, J., Panda, K., & Acharya, K. (2015). Chitosan nanoparticles: a positive modulator of innate immune responses in plants. Scientific reports, 5(1): 1-14.
Chang, C. C., Yang, M. H., Wen, H. M., & Chern, J. C. 2002. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. Journal of food and drug analysis, 10(3).
Chen, J., Zou, X., Liu, Q., Wang, F., Feng, W., & Wan, N. 2014. Combination effect of chitosan and methyl jasmonate on controlling Alternaria alternata and enhancing activity of cherry tomato fruit defense mechanisms. Crop Protection, 56: 31-36.
Divya, K., & Jisha, M. S. 2018. Chitosan nanoparticles preparation and applications. Environmental chemistry letters, 16(1): 101-112.
Di Domenico, F., Foppoli, C., Coccia, R., & Perluigi, M. 2012. Antioxidants in cervical cancer: chemopreventive and chemotherapeutic effects of polyphenols. Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease, 1822(5): 737-747.
Ali, E. F., El-Shehawi, A. M., Ibrahim, O. H. M., Abdul-Hafeez, E. Y., Moussa, M. M., & Hassan, F. A. S. 2021. A vital role of chitosan nanoparticles in improvisation the drought stress tolerance in Catharanthus roseus (L.) through biochemical and gene expression modulation. Plant Physiology and Biochemistry, 161: 166-175.
El-Tahir, K. E. D. H., & Bakeet, D. M. 2006. The black seed Nigella sativa Linnaeus-A mine for multi cures: a plea for urgent clinical evaluation of its volatile oil. Journal of Taibah University Medical Sciences, 1(1):1-19.
Hassan, F. A. S., Morsi, M. M., & Aljoudi, N. G. S. 2017. Alleviating the Adverse Effects of Salt Stress in Rosemary by Salicylic Acid Treatment. Research journal of pharmaceutical biological and chemical sciences, 8(3): 1980-1995.
Faizan, M., Rajput, V. D., Al-Khuraif, A. A., Arshad, M., Minkina, T., Sushkova, S., & Yu, F. 2021. Effect of foliar fertigation of chitosan nanoparticles on cadmium accumulation and toxicity in Solanum lycopersicum. Biology, 10(7): 666.
Farshid, A. 2017. The effect of different levels of chitosan on the vegetative yield of peppermint. Paper presented at the The Second International Conference on Modern Agreements in Agricultural Sciences, Natural Resources and Environment.
Fazeli, A., Zarei, B., & Tahmasebi, Z. 2017. The effect of salinity stress and salicylic acid on some physiological and biochemical traits of Black cumin (Nigella sativa L.). Iranian Journal of Plant Biology, 9(4): 69-84.
Sato, F., Yoshioka, H., Fujiwara, T., Higashio, H., Uragami, A., & Tokuda, S. 2004. Physiological responses of cabbage plug seedlings to water stress during low-temperature storage in darkness. Scientia Horticulturae, 101(4): 349-357.
Ghasemi, B., Hosseini, R., & Niri, F. D. 2015. The effect of cobalt and chitosan nanoparticles on the production of artemisinin and Artemisia annua in DBR and 2 SQS expressed two key genes. Genetic engineering and biosafety, 4(1): 25-39.
Gorzi, R., Bernard, F., & Reza Ghalamboran, M. 2018. The effect of chitosan nanoparticles for the production and spread of yellow pigments in root cultivation of safflower. Journal of Medicinal Plants Biotechnology, 4(First): 16-27.
Yin, H., Fretté, X. C., Christensen, L. P., & Grevsen, K. 2012. Chitosan oligosaccharides promote the content of polyphenols in Greek oregano (Origanum vulgare ssp. hirtum). Journal of agricultural and food chemistry, 60(1): 136-143.
Ismailzadeh Bahabadi, S., Sharifi, M., Safaei, Z., & Behmanesh, M. 2013. Increase the production of lignin and compounds Phenylpropanoid by chitosan in linoleum cell culture. Journal of Plant Biology, 11: 13-26.
Kabiri , R., Nasibi, F., & Farah, B. H. 2013. Study of some oxidative parameters due to drought stress in black seed plant under hydroponic cultivation. Plant process and function, 3(1): 11-19.
Khaje, H., & Naderi, S. 2014. The effect of chitosan on some properties of antioxidant enzymes and Biochemical in Melissa. Journal of Crop Science in Arid Areas, 1(1): 100-116.
Abbasi Khalaki, M., Moameri, M., Asgari Lajayer, B., & Astatkie, T. 2021. Influence of nano-priming on seed germination and plant growth of forage and medicinal plants. Plant growth regulation, 93(1): 13-28.
Khanna-Chopra, R., & Selote, D. S. 2007. Acclimation to drought stress generates oxidative stress tolerance in drought-resistant than-susceptible wheat cultivar under field conditions. Environmental and Experimental Botany, 60(2): 276-283.
Kulisic, T., Radonic, A., Katalinic, V., & Milos, M. 2004. Use of different methods for testing antioxidative activity of oregano essential oil. Food chemistry, 85(4): 633-640.
Mahdavi, B., & Rahimi, A. 2013. Seed priming with chitosan improves the germination and growth performance of ajowan (Carum copticum) under salt stress. EurAsian J BioSci 7: 69–76.
Mahdavi, S. A. 2013. Effect of different concentrations of chitosan on seed germination and antioxidant enzymes of safflower (carthamus tntorius L) under dehydration. 26(3): 365-352.
Mahmoudi, R., Tajali Ardakani, M., & Bardania, H. 2019. Cytotoxicity and apoptotic effect of chitosan nanoparticles containing hydroalcoholic extract of physalis alkekengi on HT29 cell line. Journal of Mazandaran University of Medical Sciences, 29(180): 102-107.
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