• الصفحة الرئيسية
  • بررسی فیتوشیمیایی، ارزیابی عملکرد آنتی‌اکسیدانی و آنتی-باکتریایی عصاره‌های پنج گونه دارویی Pulicaria gnaphalodes (Vent.) Boiss، Rhazya stricta L.، Salvadora persica L.، Teucrium polium L.، Hibiscus sabdariffa L. در استان سیستان و بلوچستان

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عنوان URL للمقالة


رقم المقالة : EJMP-1702-1192 (R1) زيارة : 204 الصفحة: 49 - 68

20.1001.1.23223235.1396.5.2.5.6

نوع المخطوط: ابحاث

بررسی فیتوشیمیایی، ارزیابی عملکرد آنتی‌اکسیدانی و آنتی-باکتریایی عصاره‌های پنج گونه دارویی Pulicaria gnaphalodes (Vent.) Boiss، Rhazya stricta L.، Salvadora persica L.، Teucrium polium L.، Hibiscus sabdariffa L. در استان سیستان و بلوچستان

الموضوعات :

امید عزیزیان شرمه 1 , محرم ولی زاده 2 , علی قاسمی 3 , احمد مهربان 4 , افسانه کمالی دلجو 5

1 - دانش‌آموخته کارشناسی ارشد فیتوشیمی، باشگاه پژوهشگران جوان و نخبگان، واحد زاهدان، دانشگاه آزاد اسلامی، زاهدان، ایران
2 - استادیار، گروه تولیدات گیاهی، دانشکده کشاورزی، مجتمع آموزش عالی سراوان، سراوان، ایران
3 - مربی، گروه زیست شناسی، دانشکده علوم پایه، دانشگاه سیستان و بلوچستان، زاهدان، ایران
4 - استادیار، گروه زراعت و اصلاح نباتات، دانشکده کشاورزی، دانشگاه آزاد اسلامی واحد زاهدان، زاهدان، ایران
5 - دانش آموخته کارشناسی ارشد زراعت و اصلاح نباتات، باشگاه پژوهشگران جوان و نخبگان، واحد زاهدان، دانشگاه آزاد اسلامی، زاهدان، ایران

تاريخ الإرسال : 06 الجمعة , جمادى الأولى, 1438 تاريخ التأكيد : 11 السبت , رجب, 1438 تاريخ الإصدار : 01 الأربعاء , ذو الحجة, 1438

الکلمات المفتاحية: سیستان و بلوچستان, آنتی‌اکسیدانی, فلاونوئید, فنول, آنتی‌باکتریایی, گیاهان دارویی بومی,

ملخص المقالة :

مطالعه حاضر به بررسی ترکیبات فیتوشیمیایی فنول و فلاونوئید کل، ارزیابی عملکرد آنتی اکسیدانی و آنتی باکتریایی سه عصاره متانولی، اتانولی و آبی پنج گونه مختلف گیاهان دارویی Pulicaria gnaphalodes (Vent.) Boiss.، Rhazya stricta L.، Salvadora persica L.، Teucrium polium L. و Hibiscus sabdariffa L. در منطقه سیستان و بلوچستان می پردازد. پس از جمع آوری گیاهان و تهیه عصاره ها به روش ماسراسیون، محتوای فنولی و فلاونوئیدی به‌ترتیب با روش های معرف فولین-سیوکالتیو و رنگ سنجی کلرید آلومینیوم، فعالیت آنتی اکسیدانی با دو روش DPPH و FRAP و فعالیت آنتی باکتریایی علیه باکتری های استافیلوکوکوس اورئوس، انتروکوکوس فکالیس، اشرشیاکلی و سالمونلا تیفیموریوم به روش دیسک دیفیوژن (انتشار دیسک) سنجیده شده است. نتایج نشان دادند که در تمامی گونه ها، عصاره متانولی دارای بیشترین ترکیبات ثانویه و فعالیت های آنتی‌اکسیدانی و آنتی باکتریایی بوده است. عصاره متانولی گونه Hibiscus sabdariffa L. دارای بیشترین میزان ترکیبات فنولی (mgGAE/g44/6±54/32) و فلاونوئیدی (mgGAE/g00/1±11/27) و فعالیت آنتی اکسیدانی (µg/ml21/1±34/17IC50= و mMFe2+/mg27/2±19/55) و فعالیت آنتی باکتریایی علیه باکتری استافیلوکوکوس اورئوس با قطر‌هاله عدم رشد 00/1±27 میلی متر بوده است. در مقابل عصاره آبی گونه Teucrium poliumL.دارای کمترین میزان ترکیبات فنولی (mgGAE/g11/1±33/4) و فلاونوئیدی (mgGAE/g08/0±03/2) و فعالیت آنتی‌اکسیدانی (µg/ml02/2±71/220 IC50= و mM Fe2+/mg sample99/0±76/8) و فعالیت آنتی باکتریایی علیه باکتری انترکوکوس فکالیس با قطر‌هاله عدم رشد 50/1±4 میلی متر و اشرشیاکلی با قطر‌ هاله عدم رشد 57/0±4 میلی متر بوده است. به‌طورکلی بر اساس نتایج بدست آمده، گیاهان مورد مطالعه می توانند کاندیدای خوبی جهت درمان بیماری های ناشی از استرس‌های اکسیداتیوی و بیماری های ناشی از میکروب های بیماری زا قرار گیرند.

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