بررسی اثر آللوپاتیکی علف هرز خربزه وحشی Cucumis melo L. بر رشد و سیستم آنتی اکسیدانی دو گیاه کلزا (Brassica napus L) و خردل وحشی (Sinapis arvensis)
الموضوعات :نشاط نوروزی 1 , مریم نیاکان 2 , مهدی عبادی 3 , معصومه یونس آبادی 4
1 - گروه زیست شناسی، دانشکده علوم پایه، واحد گرگان، دانشگاه آزاد اسلامی، گرگان، ایران
2 - گروه زیست شناسی، دانشکده علوم پایه، واحد گرگان، دانشگاه آزاد اسلامی، گرگان، ایران
3 - گروه شیمی، دانشکده علوم پایه، واحد گرگان، دانشگاه آزاد اسلامی، گرگان، ایران
4 - مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی استان گلستان، سازمان تحقیقات، آموزش و ترویج کشاورزی،گرگان، ایران
الکلمات المفتاحية: کاتالاز, کلزا, رشد, پراکسیداز, خردل وحشی, فنول, فلاونوئید, خربزه وحشی,
ملخص المقالة :
اثر آللوپاتیک علف های هرز یکی از عوامل مهم محدودکننده رشد و عملکرد گیاهان زراعی می باشد. هدف از انجام این پژوهش بررسی اثر عصاره آبی اندام هوایی (برگ و ساقه) خربزه وحشی Cucumis melo L. بر شاخص‌های رشد و سیستم آنتی اکسیدانی گیاه کلزا و علف هرز همراه آن یعنی خردل وحشی (Sinapis arvensis) بود. این پژوهش بصورت اسپلیت اسپلیت پلات در قالب طرح پایه بلوک های کامل تصادفی در سه تکرار در سطح مزرعه انجام شد. پلات اصلی نوع گیاه هدف در دو سطح (کلزا رقم RGS و علف هرز خردل وحشی) و پلات فرعی نوع اندام در دو سطح (عصاره آبی برگ و ساقه خربزه وحشی) و پلات فرعی فرعی غلظت عصاره در چهار سطح ((شاهد)، 5/2، 5 و %10) بود. نتایج نشان داد با افزایش غلظت عصاره برگ خربزه وحشی، اکثر پارامترهای رشد در هر دو اندام گیاهان هدف کاهش یافت و شدت این کاهش بر رشد Sinapis arvensis در مقایسه با کلزا بیشتر بود، در حالی که عصاره ساقه خربزه وحشی موجب کاهش شدیدتر پارامترهای رشد کلزا در مقایسه با Sinapis arvensis گشت. همچنین عصاره ساقه خربزه وحشی باعث افزایش معنی دار فعالیت آنزیم کاتالاز و کاهش پراکسیداز در کلزا نسبت به شاهد شد، درحالیکه فعالیت آنزیم پراکسیداز در خردل وحشی افزایش یافت. همچنین روند افزایشی ترکیبات فنولی و کاهش ترکیبات فلاونوئیدی برگ کلزا با افزایش غلظت عصاره از نظر آماری به صورت معنی دار بیشتر از خردل وحشی بود. با توجه به نتایج این تحقیق حساسیت گیاه زراعی کلزا و علف هرز همراه آن به عصاره دو اندام خربزه وحشی یکسان نبوده که این مسئله می‌بایست در مدیریت استفاده از توان آللوپاتی گیاهان به‌عنوان علف کش در سطح مزرعه مورد توجه قرار گیرد.
Ali, J.S., ul Haq, I., Ali, A., Ahmed, M. and Zia, M. (2017). Onosma bracteatum Wall and Commiphora stocksiana Engl extracts generate oxidative stress in Brassica napus: An allelopathic perspective. Cogent Biology. 3(1): 1283875.
Ali, I.B.E.H., Bahri, R., Chaouachi, M., Boussaïd, M. and Harzallah-Skhiri, F. (2014). Phenolic content, antioxidant and allelopathic activities of various extracts of Thymus numidicus Poir. organs. Industrial Crops and Products. 62: 188-195.
Al-Qudah, M.A., Al-Jaber, H.I., Muhaidat, R., Hussein, E.I., Abdel, A.A. and Hamid, A.S. (2011). Chemical composition and antimicrobial activity of the essential oil from Sinapis alba L. and Sinapis arvensis L.(Brassicaceae) growing wild in Jordan. Research Journal of Pharmaceutical, Biological and Chemical Sciences . 2(4): 1136-1144.
Asaduzzaman, M., Pratley, J.E., Luckett, D., Lemerle, D. and Wu, H. (2020). Weed management in canola (Brassica napus L): a review of current constraints and future strategies for Australia. Archives of Agronomy and Soil Science. 66(4): 427-444.
Ashraf, R., Sultana, B., Yaqoob, S. and Iqbal, M. (2017). Allelochemicals and crop management: A review. Current Science. 3(1): 1-13.
Bharwana, S.A., Ali, S., Farooq, M.A., Iqbal, N., Hameed, A., Abbas, F. and Ahmad, M.S. A. (2014). Glycine betaine-induced lead toxicity tolerance related to elevated photosynthesis, antioxidant enzymes suppressed lead uptake and oxidative stress in cotton. Turkish Journal of Botany. 38(2): 281-292.
Bogatek, R., Gniazdowska, A., Zakrzewska, W., Oracz, K. and Gawronski, S. W. (2006). Allelopathic effects of sunflower extracts on mustard seed germination and seedling growth. Biologia Plantarum. 50(1): 156-158.
Bor, M., Özdemir, F. and Türkan, I. (2003). The effect of salt stress on lipid peroxidation and antioxidants in leaves of sugar beet Beta vulgaris L. and wild beet Beta maritima L. Plant Science. 164(1): 77-84.
Cartea, M.E., Francisco, M., Soengas, P. and Velasco, P. (2011). Phenolic compounds in Brassica vegetables. Molecules Journal. 16(1): 251-280.
Chance, B. and Maehly, A.C. (1995). Assay of Catalase and Peroxidase. Methods in Enzymology, Academic Press. New York. (2): 764-775.
Cheng, F. and Cheng, Z. (2015). Research progress on the use of plant allelopathy in agriculture and the physiological and ecological mechanisms of allelopathy. Frontiers in plant science. 6: 1020.
de Mattos Ribeiro, V., Spiassi, A., Marcon, T. R., de Lima, G.P., Corsato, J.M. and Fortes, A.M.T. (2017). Antioxidative enzymes of Cucumis sativus seeds are modulated by Leucaena leucocephala extracts. Acta Scientiarum. Biological Sciences. 39(3): 373-380.
El-Shora, H.M. and Abd El-Gawad, A.M. (2015). Physiological and biochemical responses of Cucurbita pepo L. mediated by Portulaca oleracea L. allelopathy. Fresenius Environmental Bulletin Journal. 24: 386-393.
Fahamiya, N., Aslam, M., Siddiqui, A. and Shiffa, M. (2016). Review on cucumis melo: Ethnobotany and unani medcine. World Journal of Pharmaceutical Sciences. 5: 621-636.
Farhoudi, R. and Lee, D.J. (2013). Allelopathic effects of barley extract (Hordeum vulgare) on sucrose synthase activity, lipid peroxidation and antioxidant enzymatic activities of Hordeum spontoneum and Avena ludoviciana. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences. 83(3): 447-452.
Gherekhloo, J., Hatami, Z.M., Alcántara-de la Cruz, R., Sadeghipour, H.R. and De Prado, R. (2018). Continuous Use of Tribenuron-Methyl Selected for Cross-Resistance to Acetolactate Synthase–inhibiting Herbicides in Wild Mustard (Sinapis arvensis). Weed Science. 66(4): 424-432.
Gidik, B., Onemli, F. and Cabi, E. (2016). Determination of wild plant species of Brassicaceae family in Turkish Thrace. Biological Diversity and Conservation. 9: 100-105.
Gulzar, A. and Siddiqui, M.B. (2017). Allelopathic effect of Calotropis procera (Ait.) R. Br. on growth and antioxidant activity of Brassica oleracea var. botrytis. Journal of the Saudi Society of Agricultural Sciences. 16(4):375-382.
Hadadchi, G.R. and Masoudi, K.F. (2006). Allelopathic effects of aqueous extracts of Sinapis arvensis on growth and related physiological and biochemical responses of Brassica napus. Jornal of Science (university of Tehran).23(1): 23-28.
Hadi, F., Bibi, H., Razzaq, A., Iqbal, A. and Ali, G. (2016). Allelopathic effect of Cucumis melo sub-species agrestis variety Agrestis on wheat. Pakistan Journal of Weed Science Research. 22(3): 471-480.
Jabran, K. and Farooq, M. (2013). Implications of potential allelopathic crops in agricultural systems. Springer-Verlag Berlin Heidelberg.349-385.
Koroi, S.A. (1989). Gel electrophoresis tissue and spectrophotometrscho unter uchungen zomeinfiuss der temperature auf struktur der amylase and peroxidase isoenzyme. Physiology Review. 20: 15-23.
Lebecque, S., Crowet, J.M., Lins, L., Delory, B.M., du Jardin, P., Fauconnier, M.L. and Deleu, M. (2018). Interaction between the barley allelochemical compounds gramine and hordenine and artificial lipid bilayers mimicking the plant plasma membrane. Scientific reports. 8(1): 1-13.
Macías, F.A., López, A., Varela, R.M., Torres, A. and Molinillo, J.M. (2008). Helikauranoside A, a new bioactive diterpene. Journal of chemical ecology. 34(1): 65-69.
Maffei, M., Bertea, C.M., Garneri, F. and Scannerini, S. (1999). Effect of benzoic acid hydroxy-and methoxy-ring substituents during cucumber (Cucumis sativus L.) germination. I.: Isocitrate lyase and catalase activity. Plant Science. 141(2): 139-147.
M’barek, K., Zribi, I., Ullah, M.J. and Haouala, R. (2019). The mode of action of allelochemicals aqueous leaf extracts of some Cupressaceae species on lettuce. Scientia Horticulturae. 252: 29-37.
Niakan, M., Habibi, A. and GHorbanli, M. (2011). Effect of pix on germination, growth, carbohydrates and antioxidant enzymes in cotton seed. Iranian Journal of Plant Physiology. 301-307.
Quettier-Deleu, C., Gressier, B., Vasseur, J., Dine, T., Brunet, C., Luyckx, M., Cazin, M., Cazin, J.C., Bailleul, F. and Trotin, F. (2000). Phenolic compounds and antioxidant activities of buckwheat (Fagopyrum esculentum Moench) hulls and flour. Journal of ethnopharmacology. 72(1-2): 35-42.
Rekha, K. and Thiruvengadam, M. (2017). Secondary metabolite production in transgenic hairy root cultures of cucurbits. Springer International Publishing, Switzerland. 267.
Rezayian, M., Niknam, V. and Ebrahimzadeh, H. (2018). Differential responses of phenolic compounds of Brassica napus under drought stress. Plant Physiology. 8(3): 2417-2425.
Sasi Kumar, R., Priyadharshini, S., Nandha Kumar, K.P.L. and Nivedha, S. (2014). In vitro pharmacognostical studies and evaluation of bioactive constituents from the fruits of Cucumis melo L.(Muskmelon). International Journal of Pharmacognosy and Phytochemical Research. 6(4): 936-941.
Singleton, V.L. and Rossi, J.A. (1965). Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American Journal of Enology and Viticulture. 16(3): 144-158.
Wang, C., Liu, J. and Zhou, J. (2017). N deposition affects allelopathic potential of Amaranthus retroflexus with different distribution regions. Anais da Academia Brasileira de Ciências. 89(2): 919-926.
Yu, J.Q. (2001). Autotoxic potential of cucurbit crops: phenomenon, chemicals, mechanisms and means to overcome. Journal of Crop Production. 4(2): 335-348.
Zhang, Z., Zhang, Z., Han, X., Wu, J., Zhang, L., Wang, J. and Wang-Pruski, G. (2020). Specific response mechanism to autotoxicity in melon (Cucumis melo L.) root revealed by physiological analyses combined with transcriptome profiling. Ecotoxicology and Environmental Safety. 200: 110779.
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Ali, J.S., ul Haq, I., Ali, A., Ahmed, M. and Zia, M. (2017). Onosma bracteatum Wall and Commiphora stocksiana Engl extracts generate oxidative stress in Brassica napus: An allelopathic perspective. Cogent Biology. 3(1): 1283875.
Ali, I.B.E.H., Bahri, R., Chaouachi, M., Boussaïd, M. and Harzallah-Skhiri, F. (2014). Phenolic content, antioxidant and allelopathic activities of various extracts of Thymus numidicus Poir. organs. Industrial Crops and Products. 62: 188-195.
Al-Qudah, M.A., Al-Jaber, H.I., Muhaidat, R., Hussein, E.I., Abdel, A.A. and Hamid, A.S. (2011). Chemical composition and antimicrobial activity of the essential oil from Sinapis alba L. and Sinapis arvensis L.(Brassicaceae) growing wild in Jordan. Research Journal of Pharmaceutical, Biological and Chemical Sciences . 2(4): 1136-1144.
Asaduzzaman, M., Pratley, J.E., Luckett, D., Lemerle, D. and Wu, H. (2020). Weed management in canola (Brassica napus L): a review of current constraints and future strategies for Australia. Archives of Agronomy and Soil Science. 66(4): 427-444.
Ashraf, R., Sultana, B., Yaqoob, S. and Iqbal, M. (2017). Allelochemicals and crop management: A review. Current Science. 3(1): 1-13.
Bharwana, S.A., Ali, S., Farooq, M.A., Iqbal, N., Hameed, A., Abbas, F. and Ahmad, M.S. A. (2014). Glycine betaine-induced lead toxicity tolerance related to elevated photosynthesis, antioxidant enzymes suppressed lead uptake and oxidative stress in cotton. Turkish Journal of Botany. 38(2): 281-292.
Bogatek, R., Gniazdowska, A., Zakrzewska, W., Oracz, K. and Gawronski, S. W. (2006). Allelopathic effects of sunflower extracts on mustard seed germination and seedling growth. Biologia Plantarum. 50(1): 156-158.
Bor, M., Özdemir, F. and Türkan, I. (2003). The effect of salt stress on lipid peroxidation and antioxidants in leaves of sugar beet Beta vulgaris L. and wild beet Beta maritima L. Plant Science. 164(1): 77-84.
Cartea, M.E., Francisco, M., Soengas, P. and Velasco, P. (2011). Phenolic compounds in Brassica vegetables. Molecules Journal. 16(1): 251-280.
Chance, B. and Maehly, A.C. (1995). Assay of Catalase and Peroxidase. Methods in Enzymology, Academic Press. New York. (2): 764-775.
Cheng, F. and Cheng, Z. (2015). Research progress on the use of plant allelopathy in agriculture and the physiological and ecological mechanisms of allelopathy. Frontiers in plant science. 6: 1020.
de Mattos Ribeiro, V., Spiassi, A., Marcon, T. R., de Lima, G.P., Corsato, J.M. and Fortes, A.M.T. (2017). Antioxidative enzymes of Cucumis sativus seeds are modulated by Leucaena leucocephala extracts. Acta Scientiarum. Biological Sciences. 39(3): 373-380.
El-Shora, H.M. and Abd El-Gawad, A.M. (2015). Physiological and biochemical responses of Cucurbita pepo L. mediated by Portulaca oleracea L. allelopathy. Fresenius Environmental Bulletin Journal. 24: 386-393.
Fahamiya, N., Aslam, M., Siddiqui, A. and Shiffa, M. (2016). Review on cucumis melo: Ethnobotany and unani medcine. World Journal of Pharmaceutical Sciences. 5: 621-636.
Farhoudi, R. and Lee, D.J. (2013). Allelopathic effects of barley extract (Hordeum vulgare) on sucrose synthase activity, lipid peroxidation and antioxidant enzymatic activities of Hordeum spontoneum and Avena ludoviciana. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences. 83(3): 447-452.
Gherekhloo, J., Hatami, Z.M., Alcántara-de la Cruz, R., Sadeghipour, H.R. and De Prado, R. (2018). Continuous Use of Tribenuron-Methyl Selected for Cross-Resistance to Acetolactate Synthase–inhibiting Herbicides in Wild Mustard (Sinapis arvensis). Weed Science. 66(4): 424-432.
Gidik, B., Onemli, F. and Cabi, E. (2016). Determination of wild plant species of Brassicaceae family in Turkish Thrace. Biological Diversity and Conservation. 9: 100-105.
Gulzar, A. and Siddiqui, M.B. (2017). Allelopathic effect of Calotropis procera (Ait.) R. Br. on growth and antioxidant activity of Brassica oleracea var. botrytis. Journal of the Saudi Society of Agricultural Sciences. 16(4):375-382.
Hadadchi, G.R. and Masoudi, K.F. (2006). Allelopathic effects of aqueous extracts of Sinapis arvensis on growth and related physiological and biochemical responses of Brassica napus. Jornal of Science (university of Tehran).23(1): 23-28.
Hadi, F., Bibi, H., Razzaq, A., Iqbal, A. and Ali, G. (2016). Allelopathic effect of Cucumis melo sub-species agrestis variety Agrestis on wheat. Pakistan Journal of Weed Science Research. 22(3): 471-480.
Jabran, K. and Farooq, M. (2013). Implications of potential allelopathic crops in agricultural systems. Springer-Verlag Berlin Heidelberg.349-385.
Koroi, S.A. (1989). Gel electrophoresis tissue and spectrophotometrscho unter uchungen zomeinfiuss der temperature auf struktur der amylase and peroxidase isoenzyme. Physiology Review. 20: 15-23.
Lebecque, S., Crowet, J.M., Lins, L., Delory, B.M., du Jardin, P., Fauconnier, M.L. and Deleu, M. (2018). Interaction between the barley allelochemical compounds gramine and hordenine and artificial lipid bilayers mimicking the plant plasma membrane. Scientific reports. 8(1): 1-13.
Macías, F.A., López, A., Varela, R.M., Torres, A. and Molinillo, J.M. (2008). Helikauranoside A, a new bioactive diterpene. Journal of chemical ecology. 34(1): 65-69.
Maffei, M., Bertea, C.M., Garneri, F. and Scannerini, S. (1999). Effect of benzoic acid hydroxy-and methoxy-ring substituents during cucumber (Cucumis sativus L.) germination. I.: Isocitrate lyase and catalase activity. Plant Science. 141(2): 139-147.
M’barek, K., Zribi, I., Ullah, M.J. and Haouala, R. (2019). The mode of action of allelochemicals aqueous leaf extracts of some Cupressaceae species on lettuce. Scientia Horticulturae. 252: 29-37.
Niakan, M., Habibi, A. and GHorbanli, M. (2011). Effect of pix on germination, growth, carbohydrates and antioxidant enzymes in cotton seed. Iranian Journal of Plant Physiology. 301-307.
Quettier-Deleu, C., Gressier, B., Vasseur, J., Dine, T., Brunet, C., Luyckx, M., Cazin, M., Cazin, J.C., Bailleul, F. and Trotin, F. (2000). Phenolic compounds and antioxidant activities of buckwheat (Fagopyrum esculentum Moench) hulls and flour. Journal of ethnopharmacology. 72(1-2): 35-42.
Rekha, K. and Thiruvengadam, M. (2017). Secondary metabolite production in transgenic hairy root cultures of cucurbits. Springer International Publishing, Switzerland. 267.
Rezayian, M., Niknam, V. and Ebrahimzadeh, H. (2018). Differential responses of phenolic compounds of Brassica napus under drought stress. Plant Physiology. 8(3): 2417-2425.
Sasi Kumar, R., Priyadharshini, S., Nandha Kumar, K.P.L. and Nivedha, S. (2014). In vitro pharmacognostical studies and evaluation of bioactive constituents from the fruits of Cucumis melo L.(Muskmelon). International Journal of Pharmacognosy and Phytochemical Research. 6(4): 936-941.
Singleton, V.L. and Rossi, J.A. (1965). Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American Journal of Enology and Viticulture. 16(3): 144-158.
Wang, C., Liu, J. and Zhou, J. (2017). N deposition affects allelopathic potential of Amaranthus retroflexus with different distribution regions. Anais da Academia Brasileira de Ciências. 89(2): 919-926.
Yu, J.Q. (2001). Autotoxic potential of cucurbit crops: phenomenon, chemicals, mechanisms and means to overcome. Journal of Crop Production. 4(2): 335-348.
Zhang, Z., Zhang, Z., Han, X., Wu, J., Zhang, L., Wang, J. and Wang-Pruski, G. (2020). Specific response mechanism to autotoxicity in melon (Cucumis melo L.) root revealed by physiological analyses combined with transcriptome profiling. Ecotoxicology and Environmental Safety. 200: 110779.
