اثرپیش تیمار با غلظت‌های مختلف اوره بروی خصوصیات فیزیولوژیکی و بیوشیمایی ذرت (Zea mays L.) تحت تنش شوری
الموضوعات :
هما زارعی
1
,
محمد صدقی
2
,
سلیم فرزانه
3
,
هانیه سعادت
4
1 - دانشجوی کارشناسی ارشد علوم و تکنولوژی بذر، دانشگاه محقق اردبیلی دانشکده کشاورزی و منابع طبیعی، اردبیل، ایران،
2 - استاد گروه علوم و مهندسی تولید و ژنتیک گیاهی، دانشکده کشاورزی و منابع طبیعی، دانشگاه محقق اردبیلی، اردبیل، ایران.
3 - استادیار گروه زراعت و اصلاح نباتات دانشگاه محقق اردبیلی
4 - دکتری اکولوژی، دانشگاه محقق اردبیلی، دانشکده کشاورزی و منابع طبیعی، گروه زراعت و اصلاح نباتات
الکلمات المفتاحية: آنزیم‌های آنتی‌اکسیدانت, اوره, پرایمینگ, کلرید سدیم,
ملخص المقالة :
به منظور بررسی اثر پیش‌تیمار با غلظت‌های مختلف اوره روی خصوصیات فیزیولوژیکی و بیوشیمیایی ذرت تحت تنش شوری، آزمایشی به‌صورت فاکتوریل در قالب طرح کاملا تصادفی در سه تکرار در دانشگاه محقق اردبیلی در سال 1401 اجرا گردید. فاکتورهای مورد بررسی سطوح مختلف شوری (صفر، 100 و ۲۰۰ میلی‌مولار) و سطوح مختلف محلول اوره (صفر، 5/1 و ۳ درصد) بود. نتایج نشان داد شوری درصدجوانه‌زنی، سرعت جوانه‌زنی، یکنواختی جوانه‌زنی، طول ریشه‌چه و ساقه‌چه، وزن تر و خشک ریشه‌چه و ساقه‌چه را کاهش داد. ولی پیش‌تیمار با اوره این صفات را بهبود بخشید. بیش‌ترین میانگین مدت جوانه‌زنی در شوری200 میلی‌مولار و شاهد (آب مقطر) مشاهده شد. فعالیت آنزیم‌های کاتالاز و پراکسیداز با تشدید شوری افزایش یافتند و بیش‌ترین مقدار آن‌ها در شوری 200 میلی‌مولار مشاهده شد. پیش‌تیمار با محلول اوره 3 درصد این آنزیم‌ها را بهبود بخشید. فعالیت آنزیم سوپراکسید دیسمیوتاز در پرایمینگ با اوره 3 درصد و شوری200 میلی‌مولار نسبت به شاهد حدود 61 درصد افزایش نشان داد. آنزیم آمیلاز و پروتئین در پیش‌تیمار با اوره 3 درصد و بدون شوری نسبت به شاهد به ترتیب حدود 73 و 70 درصد افزایش نشان دادند. با توجه به نتایج مشاهده شده بذرهای پرایم شده با محلول اوره 3 درصد بیش‌ترین تاثیر را در شرایط تنش شوری روی ذرت داشتند.
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