اثر تنشهای خشکی، شوری و تیمار اسیدآبسیزیک بر خصوصیات جوانهزنی گیاه دارویی شاهدانه (Cannabis sativa L.)
الموضوعات :علی صالحی ساردویی 1 , هاجر معتمدی شارک 2 , خدایار همتی 3
1 - گروه علوم باغبانی، دانشگاه کشاورزی و منابع طبیعی گرگا
2 - گروه علوم باغبانی دانشگاه کشاورزی و منابع طبیعی گرگان
3 - گروه علوم باغبانی، دانشگاه کشاورزی و منابع طبیعی گرگان
الکلمات المفتاحية: بنیه بذر, ریشهچه, ساقهچه, اسیدآبسیزیک,
ملخص المقالة :
بهمنظور بررسی اثر اسیدآبسیزیک بر خصوصیات جوانهزنی بذر شاهدانه در شرایط یکسان خشکی و شوری، دو آزمایش مستقل همزمان در آزمایشگاه گروه علوم باغبانی دانشگاه علوم کشاورزی و منابع طبیعی گرگان در قالب طرح کاملا تصادفی با چهار سطح اسیدآبسیزیک (0، 5، 10 و 20 میلیگرم در لیتر) و پنج سطح تنش (0، 5/1-، 3-، 5/4- و 6- بار) بهترتیب برای تنش خشکی و شوری در سه تکرار انجام شد. از محلولهای PEG و NaCl بهترتیب برای ایجاد تنش خشکی و شوری استفاده گردید. نتایج نشان داد که با افزیش تنش خشکی و شوری بهطور معنیداری از درصد جوانهزنی، سرعت جوانهزنی، شاخص بنیه بذر، طول ریشهچه و ساقهچه، وزن تر و خشک گیاهچه کاسته شد. اسیدآبسیزیک اثر معنیداری بر صفات مورد ارزیابی داشت بهطوری که در غلظت بالا اثر منفی بر خصوصیات جوانهزنی داشت. اثر متقابل اسیدآبسیزیک و تنش خشکی و شوری (به جز وزن تر گیاهچه) اثر معنیداری بر تمام صفات مورد ارزیابی در سطح یک درصد نشان داد. بهطور کلی، نتایج نشان داد که جوانهزنی بذرهای شاهدانه تا حدودی شرایط تنش خشکی را بهتر از شرایط شوری تحمل میکند. همچنین تیمار اسیدآبسیزیک بسته به سطح تنش و غلظت استفاده شده، اثر متفاوتی داشت بهطوری که غلظتهای پایین باعث بهبود خصوصیات جوانهزنی شد.
References
Abdi, H., Bihamta, M.R., Aziz-Ov, E. and Chogan, R. 2015. Investigation effect of drought stress level of PEG 6000 on seed germination principle and its relation with drought tolerance index in promising lines and cultivars of bread wheat (Triticum. aestivum L.). Iranian Journal of Field Crops Research, 12(4): 582-596. (In Persian)
Aghighi Shahverdi, M., Memivand, B. and Ataei Somagh. H. 2014. Effects of seed primingwithplant growth promoting bacteria on germination indices under alt stress Basil. Seed Research, 4(4): 38-50. (In Persian)
Alizadeh Ahmadabadi, A. and khorasaninejad, S. 2016. The Effect of Humic acid Pretreatment on Germination of purple cornflower (Echinacea purpurea) plant under Drought and Salinity Conditions. Arid Biome Scientific and Research Journal, 6(2): 97-106. (In Persian)
Amiri, M.B., Rezvani Moghadam, P., Ehyaii, H.R., Falahi, J. and Aghhavani Shajari, M. 2011. The Effect of osmotic stress and salinity on germination indices and seedling growth of two medicinal plants (Cynara scoolymus) and (Echinacea purpurea). Journal of Environmental Stresses in Crop sciences, 2(3): 165-176. (In Persian)
Asghari, M. 1993. The Effect of ethylene on osmotic regulation and growth of axial and cotyledon tissues of sunflower seeds under drought stress conditions. Agricultural Sciences and Technology Journal, 7: 137-145. (In Persian)
Ashraf, M., Athar, H.R., Harris, P.J.C. and Kwon, T.R. 2008. Some prospective strategies for improving crop salt tolerance. Advan. Agron., 97: 45–110.
Baghdadi, A., Ashraf Jafari, A., Alizade, M.A. and Gorji, A. 2014. The Effect of drought and cold stress on germination and seedling growth in populations of Poa trivialis and Poa pratensis in germinator and greenhouse conditions. Iranian Journal of Range and Desert Research, 20(4): 706-719.(In Persian)
Chandra, S., Lata, H. and ElSohly, M.A. 2017. Cannabis sativa L- Botany and Biotechnology, 1st ed.; Springer International Publishing AG: Cham, Switzerland, pp. ix–xi.
Covarrubias, A.A. and Reyes, J.L. 2010. Post- transcriptional gene regulation of salinity and drought responses by plant microRNAs. Plant, Cell and Environment, 33(4): 481-489.
Cutt, J.R. and Klessig, D.F. 1992. Salicylic acid in plants: A changing perspective. Pharmaceutical Technology, 16: 25–34.
Dadkhah, A.R. 2011. Study of the effect of salt stress and salt Type on germination and seedling growth of four medicinal plants of Fenugreek, Sesame, Cannabis and Zinnian. Iranian Journal of Medical and Aromatic Plants, 26(3): 319-358. (In Persian)
Ebadi, M.T., Azizi, M. and Farzaneh, A. 2011. Effect of drought stress on germination factors of four improved cultivars of German chamomile (Matricaria recutita L.). Journal of Plant Production, 18(2): 119-131. (In Persian)
Ehteshamnia, A. 2007. The effects of salinity on seedling growth of 10 medicinal plant components. Conference Medicinal Plants. Shahed University Tehran. November. P123. (In Persian)
Fakheri, B.A., Mousavi Nick, S.M. and Mohammadpour Vashvaei, R. 2017. Effect of drought stress induced by polyethylene glycol on germination and morphological properties of fennel and ajowan. Journal of Crop Science Research in Arid Regions, 1(1): 35-50. (In Persian).
Finch-Savage, W.E. and Leubner-Metzger, G. 2006. Seed dormancy and the control of germination. New Phytologist, 171: 501–523.
Ghasemi, A.A., Hamidi, H., Aroos, J. and Masoomi, A. 2014. The Effect of Salinity and Temperature on Hyssopus officinalis Germination. Journal of Crop Improvement, 15(3): 155-169. (In Persian)
Gholinezhad, E. 2017. The effect of different levels of drought stress on traits related to germination and seedling growth of dill (Anethum graveolens L.). Journal of Seed Research, 6(4): 57-71. (In Persian)
Ghonavati, M., Hooshmand, S. and Zeynali, H. 2007. Study the effect of different levels of salinity on germination of two species of chamomile. 9th Iranian Crop Sciences Congress. 597.
Ghorbani Javid, M., Sorooshzadeh, A., Moradi, F., Modarres Sanavy, S.A.M. and Allahdadi, I. 2011. The role of phytohormones in alleviating salt stress in crop plants. Crop Science, 5(6): 726-734.
Gianinetti, A. and Vernieri, P. 2007. On the role of abscisic acid in seed dormancy of red rice. Journal of Experimental Botany, 58: 3449-3462.
Goggin, D.E., Steadman, K.J., Neil Emery, R.J., Farrow, S.C., Benech-Arnold, R.L. and Powels, S.B. 2009. ABA inhibits germination but not dormancy release in mature imbibed seeds of Lolium rigidum Gaud. Journal of Experimental Botany, 60: 3387-3396.
Hassani, A. 2005. Polyethylene glycol induced water stress on basil seed. Journal of Medicinal and Aromatic Plants Research of Iran, 4(21): 535‐544. (In Persian)
Hazekamp, A. 2009. Cannabis review. Depertment of plant metabolomics Leiden University. Leiden the Netherland.
Iqbal, M. and Ashraf, M. 2010. Gibberellic acid mediated induction of salt tolerance in wheat plants: Growth, ionic partitioning, photosynthesis, yield and hormonal homeostasis. Environmental and Experimental Botany, 6(1): 521-536.
Javadi, H., Segheh Al Eslami, M.J. and Moosavi, Gh.R. 2014. Study of Salinity Effects on Germination and Seedling Growth of Four Medicinal Plants. Iranian Journal of Field Crops Research, 12(1): 53-64. (In Persian)
Kafi, M., Nezami, A., Hosseini, H. and Massumi, A. 2005. The physiological effects of drought stress induced by PEG 6000 on germination of lentil genotypes. Iranian Journal of Field Crops Researches, 3: 69-81. (In Persian)
Karenzhadi, A., Galeshi, S., Zeynali, A. and Zangi, M.R. 2005. Study of salinity tolerance of thirty cotton genotypes at germination stage. Agricultural Sciences and Technology Journal, 18(1): 109-126. (In Persian)
Kauser, A. and Azam, F. 1985. Effect of humic acid on wheat seeding growth. Environmental and Experimental Botany, 25: 245–252.
Kermode, A.R. 2005. Role of abscisic acid in seed dormancy. Journal of Plant Growth Regulation. 24: 319–344.
Koo, E.S. 2006. Humic acid or fulvic acid: which organic acid accelerates the germination of the green mung beans? California State Science Fair. 1617.
Kucera, B., Cohn, M.A. and Leubner-Metzger, G. 2005. Plant hormone interactions during seed dormancy release and germination. Seed Science Research, 15: 281-307.
Macar, T.K., Turan O. and Ekmekci, Y. 2009. Effects of water deficit induced by PEG and NaCl on chickpea (Cicer arietinum L.) cultivars and lines at early seedling stages. G.U. Journal of Science, 22: 5-14.
Mallikarjuna, M., Govindasamy, R. and Chandrasekaran, S. 1987. Effect of humic acid on sorghum vulgare var.CSH-9. Current Sciences, 56:12-73.
Mansoori, H. and Asrar, Z. 2014. The Effect of ABA on pigments and Tetrahydrocannabinol Cannabis sativa in flowering stage. Iranian Journal of Biology, 26(1): 82-88. (In Persian)
McCarty, D.R. 1995. Genetic control and integration of maturation and germination pathways in seed development. Annual Review of Plant Physiology and Plant Molecular Biology. 46: 71–93.
Najafpoor Navai, M., Sefid Kan, F.V. and Mirza, M. 2008. Introduction of Iranian Anticancer Drug Herbs. First edition, Research Institute for Forests and Rangelands of the Country, Agricultural Research and Education agricultural, Ministry of Agricultural Jihad, 34-35.
Nambara, E., Okamoto, M., Tatematsu, K., Yano, R., Seo, M. and Kamiya, Y. 2010. Abscisic acid and the control of seed dormancy and germination. Seed Science Research, 20: 55–67.
Nasrollah, S. 2015. The effect of electromagnetic waves on cannabis. Master thesis, Department of Biology, Faculty of Biology, Islamic Azad University, North Tehran Branch.
Ogawa, M., Hanada, A., Yamauchi, Y., Kuwahara, A., Kamiya, Y. and Yamaguchi, S. 2003. Gibberellin biosynthesis and response during Arabidopsis seed germination. The Plant Cell, 15: 1591-1604.
Okamoto, M., Kuwahara, A., Seo, M., Kushiro, T., Asami, T., Hirai, N., Kamiya, Y., Koshiba, T. and Nambara, E. 2006. CYP707A1 and CYP707A2, which encode ABA 80-hydroxylases, are indispensable for a proper control of seed dormancy and germination in Arabidopsis. Plant Physiology, 141: 97–107.
Okamoto, M., Tatematsu, K., Matsui, A., Morosawa, T., Ishida, J., Tanaka, M., Endo, T., Mochizuki, Y., Toyoda, T., Kamiya, Y., Shinozaki, K., Nambara, E. and Seki, M. 2010. Genome-wide analysis of endogenous abscisic acid-mediated transcription in dry and imbibed seeds of Arabidopsis using tiling arrays. Plant Journal, (In Press).
Omidi, H. 2010. Changes of Proline Content and Activity of Antioxidative Enzymes in Two Canola Genotype under Drought Stress. American Journal of Plant Physiology, 5: 338-349.
Pinarkara, E., Kayis, A.S., Hakki, E. and Sag, A. 2004. RAPD analysis of seizes marijuana (Cannabis sativa L.) in Turkey. Electronic Journal of Biotechnology, 12: 1-13.
Ramezani, A., Ghajar Sepanlo, M. and Naghdi Badi, H.A. 2012. Evaluation of germination potential of Echium amoenum Fisch. & Mey seedlings in salt conditions. Watershed Management Research, 91: 80-87. (In Persian)
Safar Nezhad, A. and Hamidi, H. 2009. Study of Morphological Characteristics of Fennel (Foeniculum vulgare Mill.) under Salt Stress. Iranian Journal of Rangelands Forests Plant Breeding and Genetic Research, 16(1): 125-140. (In Persian)
Saidi Poor, S., Moradi, F., Nabi Poor, M. and Rahimi Fard, M. 2007. The Effect of NaCl-induced salinity stress on the variation and distribution of ABA and IAA in seedlings of tolerant (IR 651) and sensitive (IR29) rice genotypes. Iranian Journal of Crop Sciences, 8(3): 215-231. (In Persian)
Sanginabadi, H. and khorasaninejad, S. 2016. The Effect of Drought, Salinity Stresses and salicylic acid Pretreatment on seed germination factors Lavandula stricta Del. plant. Journal of Horticultural Science, 30(3): 423-430. (In Persian)
Seyedi, M., Hamzei, J., Bourbour, A., Dadrasi, V. and Sadeghi, F. 2013. The Effect of hydro-priming on germination properties and seedling growth of the safflower (Carthamus tinctorius L.) under drought stress. Journal of Agronomy Sciences, 5(8): 63-76. (In Persian)
Sharifi, H., Goldani, M., Ghias-Abadi, M. and Sharifi, Z. 2017. The effect of seed coat colour on mustard (Brassica compestris Var. Parkland) seed germination under salinity and drought stress. Iranian Journal of Seed Science and Research, 3(4): 57-67. (In Persian)
Sharp, R.E. 2002. Interaction with ethylene: changing views on the role of abscises acid in root and shoot growth response to water stress. Plant, Cell and Environment, 25: 211–222.
Soltani, E., Akram-Ghaderi, F. and Soltani, A. 2008. Applications of germination modeling on the response to temperature and water potential in seed Science Research. 1st National Conference of Seed Sciences and Technology in Iran. Gorgan, Iran. 445p.
Takel, A. 2000. Seedling emergence and growth of sorghum genotypes under variable soil moisture deficit. Agronomy Journal. 48: 95-102.
Tavakol Afshari, R., Badri, S. and Abasi, A.R. 2011. Study of the effect of gibberellin and Abscisic acid on germination, induction of dormancy and activity of the enzymes of acid and alkaline phosphatase in wheat seed embryos (Triticum aestivum) variety RL4137. Iranian Journal of Field Crop Science, 41(4): 781-789. (In Persian)
Tsegazeabe, H.H. and Teferii, G. 2012. The Effect of salinity stress on germination of chickpea (Cicer arietinum L.) land race of tigray. Current Research Journal of Biological Sciences, 4: 578-583.
Turk, M.A., Tahawa, A.R.M. and Lee, K.D. 2004. Seed germination and seedling growth of three lentil cultivars under moisture stress. Asian Journal of Plant Sciences, 3: 394-397.
Voigt, E.L., Almeida, T.D., Chagas, R.M., Ponte, L.F.A., Viégas, R.A. and Silveira, J.A.G. 2009. Source–sink regulation of cotyledonary reserve mobilization during cashew (Anacardium occidentale) seedling establishment under NaCl salinity. Journal of Plant Physiology, 166: 80–89.
Yurekli, F., Banu Porgali, Z. and Turkan, I. 2004. Variations in abscisic acid, indole-3-acetic acid, Gibberellic acid and zeatin concentrations in two bean species subjected to salt stress. Acta biologica cracoviensia Series Botanica, 46(1): 201–212.