The effect of low-power laser on the promotion of spermatogenesis in a mouse model of azoospermia (in-vivo)
habib tajalli
1
(
Biophotonics Research center
)
Masoud Maleki
2
(
Department of Basic Science, Biotechnology Research Center, Tabriz Branch, Azad Islamic University, Tabriz, Iran
)
Esmail Safavi
3
(
Department of Basic Sciences, Faculty of Veterinary Medicine, Tabriz Branch, Azad Islamic University, Tabriz, Iran
)
Reza Shahi
4
(
Department of Mathematics and Statistics, Tabriz branch, Islamic Azad University, Tabriz, Iran
)
Fatemeh Firoozi
5
(
Department of Basic Science, Biotechnology Research Center, Tabriz Branch, Azad Islamic University, Tabriz, Iran
)
Zahra Akbarpour
6
(
Department of Basic Science, Biotechnology Research Center, Tabriz Branch, Azad Islamic University, Tabriz, Iran
)
Ali reza Sotoudeh Khyaban
7
(
Research Center of Biophotonics, Islamic Azad University, Tabriz Branch, Tabriz, Iran
)
الکلمات المفتاحية: Infertility, Spermatogenesis, azoospermia, Laser therapy, Busulfan, low power laser,
ملخص المقالة :
This research aimed to investigate the effect of low power laser on spermatogenesis in testicular tissue of azoospermia mouse model in-vivo. In this experimental work, 112 adult male Syrian mice were randomly divided into three groups (harboring 8 mice each): negative control, positive (Azoospermia control) group, and experimental group. To create azoospermia in positive animals, Busulfan was used at a dose of 30mg/kg, for 21 days by intraperitoneal injection. In the experimental group after Busulfan treatment, they were affected by low power diode laser (wavelength of 808nm) at three different energy densities of 2, 4, and 8 J/cm2. The employment of a laser with an energy density of 8 J/cm2 was shown to be beneficial in boosting germ cell and sperm production.
