بررسی سطح پلاسمایی هپسیدین، سیستاتین سی و ژلسولین در تیلریوز گوسفندی (نژاد قزل) بر اساس سن و شدت پارازیتمی
الموضوعات :کاوه عظیم زاده 1 , سینا ایزدمهر 2
1 - استادیار، گروه علوم درمانگاهی، واحد ارومیه، دانشگاه آزاد اسلامی، ارومیه، ایران
2 - دانش‌آموخته دکترای حرفه ای دامپزشکی، واحد ارومیه، دانشگاه آزاد اسلامی، ارومیه، ایران
الکلمات المفتاحية: تیلریوز گوسفندی, هپسیدین, سیستاتین سی, ژلسولین,
ملخص المقالة :
بیماری تیلریوز یکی از معضلات مهم صنعت دامداری در اکثر نقاط جهان و ایران بوده که نقش مهمی در کاهش تولید شیر و گوشت داشته و در ضمن باعث ضایعات گسترده خونی و بافتی می‌گردد. هدف از این مطالعه بررسی تغییرات پلاسمایی هپسیدین، سیستاتین سی و ژلسولین در گوسفندان نژاد قزل مبتلا به تیلریوز می‌باشد. در این مطالعه پس از اخذ نمونه خون از گروه‌های بیمار و سالم، اقدام به تعیین فراسنجه های خونی اعم ازهموگلوبین، هماتوکریت، گلبول های قرمزو گلبول های سفید گردید، سپس فراسنجه ها ی پلاسمایی فوق نیز با کیت‌های اختصاصی و به کمک تکنیک الایزا اندازه‌گیری شدند. نتایج حاکی از افزایش معنی‌دار(01/0p<) هپسیدینو سیستاتین سی و کاهش معنی‌دار (01/0p<) ژلسولین در گروه بیمار (30 رأس) نسبت به گروه سالم (30 رأس) بود. همچنین، افزایش سن و شدت پارازیتمی، باعث تشدید تغییرات شدند، به‌طوری که بیشترین غلظت هپسیدین و سیستاتین سی و بیشترین تعداد لنفوسیت‌ها در گروه سنی 4 ساله و با شدت پارازیتمی بیش از 4 درصد و کمترین غلظت ژلسولین و سایر فراسنجه های هماتولوژیک نیز در همان گروه سنی و با همان شدت پارازیتمی مشاهده شد. با توجه به نتایج بدست آمده، به احتمال زیاد التهاب و آسیب سلولی گسترده در گوسفندان مبتلا به تیلریوز در بافت‌های مختلف و به‌خصوص در کلیه ها وجود دارد.
المصادر:
Almy, F.S.,Christopher, M.M.,King, D.P. and Brown S.A.(2002). Evaluation of cystatin C as an endogenous marker of glomerular filtration rate in dogs. Journal of Veterinary Internal Medicine, 16(1): 45-51.
Antognoni,M.T., Siepi,D., Porciello,F., Rueca, F. and Fruganti, G. (2007). Serum cystatin C evaluation in dogs affected by different diseases associated or not with renal insufficiency. VeterinaryResearchCommunications, 31(1):269-271.
Antognoni, M.T., Siepi, D., Porciello, F. and Fruganti, G. (2005). Use of serum cystatin C determination as a marker of renal function in the dog. Veterinary Research Communication, 29: 265-267.
Ashrafian, H. (2003). Hepcidin the missing link between hemochromatosis and infections. Infection and Immunity, 71: 6693-6700.
Bostom, A.G., Gohh, R.Y., Bausserman, L., Hakas, D. and Jacques, P.F. (1999). Serum cystatin C as a determinant of fasting total homocysteine levels in renal transplant recipients with a normal serum creatinine. Journal of American Society Nephrology, 10: 164-166.
Col, R. and Uslu, A. (2007). Changes in selected serum components in cattle naturally infected with Theileria annulata. Bulletin Veterinaria Instittute Pulawy, 15: 15-18.
Dede, S., Deger, Y., Deger, S. and Tanritanir, P. (2008). Plasma Levels of zinc, copper, copper/zinc ratio, and activity of carbonic anhydrase in equine piroplasmosis. Biological Trace Element Research, 125: 41-45.
DiNubile, M., Antin, J., Bressler, S., Stossel, T. and Ferrara, J. (1998). Decreased gelsolin levels are associated with interstitial pneumonia after allogeneic BMT. Blood, 92: 683a.
Filler, G., Bokenkamp, A., Hofmann, W., Le Bricon, T., Martinez-Bru, C. and Grubb, A. (2005). Cystatin C as a marker of GFR-history, indications, and future research. Clinical Biochemistry, 38: 1-8.
Ganz, T. (2003). Hepcidin, a key regulator of iron metabolism and mediator of anemia of inflammation. Blood, 102(3): 783-788.
Gao, Y.L., Yin, H., Luo, J.X., Ouyang, W.Q., Bao, H.M., Guan, G.Q., et al. (2002). Development of an enzyme linked immunosorbent assay for the diagnosis of Theileria sp. infection in sheep. Parasitology Research, 88: 8-10.
Hashemi-Fesharaki, R. (1998). Control of Theileria annulata in Iran. Parasitology Today, 4: 36-40.
Hashemi-Fesharaki, R. (1997). Tick-borne diseases of sheep and goats and their related vectors in Iran. Parasitologia, 39: 115-117.
Herget-Rosenthal, S., Bökenkamp, A. and Hofmann, W. (2007). How to estimate GFR-serum creatinine, serum cystatin C or equations? Clinical Biochemistry, 40: 153-161.
Howard, C.T., McKakpo, U.S., Quakyi, I.A., Bosompem, K.M. and Addison, E.A. (2007). Relationship of hepcidin with parasitemia and anemia among patients with uncomplicated Plasmodium falciparum malaria in Ghana. The American Journal of Tropical Medicine and Hygiene, 77(4): 623-626.
Hui, Q.S., Masaya, Y., Marisan, M. and Yin, H.L. (1999). Gelsolin, a Multifunctional Actin Regulatory Protein. Journal of Biological Chemistry, 274: 33179-33182.
Hu, Y., Li, H., Li, W.H., Meng, H.X., Fan, Y.Z., et al. (2013). The value of decreased plasma gelsolin levels in patients with systemic lupus erythematosus and rheumatoid arthritis in diagnosis and disease activity evaluation. Lupus, 22(14): 1455-1461.
Jensen, A.L.,Bomholt, M. and Moe, L.(2001). Preliminary evaluation of a particle-enhanced turbidimetric immunoassay (PETIA) for the determination of serum cystatin C-like immunereactivity in dogs. Veterinary Clinical Pathology, 30(2): 86-90.
Kanda, J., Mizumoto, C., Kawabata, H., Tsuchida, H., Tomosugi, N. and Matsuo, K. (2008). Serum hepcidin level and erythropoietic activity after hematopoietic stem cell transplantation. Haematologica, 93: 1550-1554.
Kinnaird, J.H., Weir, W., Durrani, Z., Pillai, S.S., Baird, M. and Shiels, B.R. (2013). A bovine lymphosarcoma cell line infected with Theileria annulata exhibits an irreversible reconfiguration of host cell gene expression. PLoS One, 8(6): e66833.
Lassus, J. and Harjola, V.P. (2012). Cystatin C: a step forward in assessing kidney function and cardiovascular risk. Heart Failure Reviews, 17: 251-261.
Lewis, D. and Purnell, R.E. (1981). The piroplasm Theileria ovis detected in sheep in south wales. Veterinary Record, 108(3): 56-57.
Marrocco, C., Rinalducci, S., Mohamadkhani, A., D'Amici, G.M. and Zolla L. (2010). Plasma gelsolin protein: a candidate biomarker for hepatitis B-associated liver cirrhosis identified by proteomic approach. Blood Transfusion, 8(3): 105-112.
Mehta, H.K., Sisodia, R.S. and Misraula, R.S. (1988). Clinical and haematological observations in experimentally induced cases of bovine theileriosis. Indian Journal of Animal Science, 58: 584-587.
Mei, L., Zheng, S., Shengbin, X. and Enyun, S. (2014). Predictive value of serum gelsolin in hepatitis B virus (HBV)-related chronic liver disease. African Journal of Biotechnology, 11(20): 4640-4645.
Miyagawa, Y., Takemura, N. and Hirose, H. (2009). Evaluation of the measurement of serum cystatin C by an enzyme-linked immunosorbent assay for humans as a marker of the glomerular filtration rate in dogs. Journal of Veterinary Medicine Science, 71: 1169-1176.
Oge, H., Doganay, A., Oge, S. and Yildirim, A. (2003). Prevalence and distribution of Dirofilaria immitis in domestic dogs from Ankara and vicinity in Turkey. Dtsch Tierarztl Wochenschr, 110: 69-72.
Park, C.H., Valore, E.V., Waring, A.J. and Ganz, T. (2001). Hepcidin, a urinary antimicrobial peptide synthesized in liver. Journal of Biological Chemistry, 276: 7806-7810.
Pasa, S., Bayramli, G., Atasoy, A., Karul, A., Ertug, S. and Ozensoy Toz, S. (2009). Evaluation of serum cystatin-C in dogs with visceral leishmaniasis. Veterinary Research Communications, 33(6): 529-534.
Pekmezci, D., Guzel, M., Yildirim, A., Ciftci, G., Pekmezci, G.Z., Tutuncu, M., et al. (2015a). Evaluation of serum cystatin-C concentrations in dogs infected with Dirofilaria immitis. Ankara Universitesi Veteriner Fakultesi Dergisi, 62(4): 303-306.
Pekmezci, D., Ural, K., Aysul, N., Guzel, M. and Ciftci, G. (2015b). Assessment of renal function using canine cystatin- C levels in canine babesiosis and ehrlichiosis. Acta Veterinaria-Beograd, 65(1): 56-65.
Randers, E. and Erlandsen, E.J. (1999). Serum cystatin C as an endogenous marker of the renal function – A Review. Clinical Chemistry Laboratory Medicine, 37: 389-395.
Rayula, V. and Hafeez, M.D. (1995). Haematological values in cattle infected with Theileria annulata. Indian Journal of Animal Science, 56: 1202-1203.
Singh, A., Singh, J., Grewal, A.S. and Brar, R.S. (2001). Studies on some blood parameters of crossbred calves with experimental Theileria annulata infections. Veterinary Research Communication, 25: 289-300.
Smith, D.B., Janmey, P.A., Sherwood, J.A., Howard, R.J. and Lind, S.E. (1988). Decreased plasma gelsolin levels in patients with Plasmodium falciparum malaria: a consequence of hemolysis? Blood, 72(1): 214-218.
Spottiswoode, N., Patrick, E.D. and Drakesmith, H. (2014). Iron, anemia and hepcidin in malaria. Front Pharmacology, 5: 125.
Soulsby, E.J.L. (1982). Helminths, Arthropods and Protozoa of Domesticated Animals. 7th ed., London: ELBS, Bailliere Tindall, pp: 728-741.
Van Velthuysen, M.L.F. and Florquin, S. (2000). Glomerulopathy associated with parasitic infections." Clinical Microbiology Reviews, 13(1): 55-66.
Villa, P., Jiménez, M., Soriano, M.C., Manzanares, J. and Casasnovas, P. (2005). Serum cystatin C concentration as a marker of acute renal dysfunction in critically ill patients. Critical Care, 9: 139-143.
Yilmaz, E., Ayarci, A.O., Sigirli, D., Torlar, M.O., Budak, F. and Goral, G. (2014). increased serum hepcidin levels in brucellosis. Clinical Laboratory, 60(11): 1837-1843.
Young, C.L., Feierstein, A. and Southwick, F.S. (1994). Calcium regulation of actin filament capping and monomer binding by macrophage capping protein. Journal of Biological Chemistry, 269(19): 13997-14002.
Zhao, D.Q., Wang, K., Zhang, H.D. and Li, Y.J. (2013). Significant reduction of plasma gelsolin levels in patients with intracerebral hemorrhage. Clinica Chimica Acta, 415: 202-206.
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Almy, F.S.,Christopher, M.M.,King, D.P. and Brown S.A.(2002). Evaluation of cystatin C as an endogenous marker of glomerular filtration rate in dogs. Journal of Veterinary Internal Medicine, 16(1): 45-51.
Antognoni,M.T., Siepi,D., Porciello,F., Rueca, F. and Fruganti, G. (2007). Serum cystatin C evaluation in dogs affected by different diseases associated or not with renal insufficiency. VeterinaryResearchCommunications, 31(1):269-271.
Antognoni, M.T., Siepi, D., Porciello, F. and Fruganti, G. (2005). Use of serum cystatin C determination as a marker of renal function in the dog. Veterinary Research Communication, 29: 265-267.
Ashrafian, H. (2003). Hepcidin the missing link between hemochromatosis and infections. Infection and Immunity, 71: 6693-6700.
Bostom, A.G., Gohh, R.Y., Bausserman, L., Hakas, D. and Jacques, P.F. (1999). Serum cystatin C as a determinant of fasting total homocysteine levels in renal transplant recipients with a normal serum creatinine. Journal of American Society Nephrology, 10: 164-166.
Col, R. and Uslu, A. (2007). Changes in selected serum components in cattle naturally infected with Theileria annulata. Bulletin Veterinaria Instittute Pulawy, 15: 15-18.
Dede, S., Deger, Y., Deger, S. and Tanritanir, P. (2008). Plasma Levels of zinc, copper, copper/zinc ratio, and activity of carbonic anhydrase in equine piroplasmosis. Biological Trace Element Research, 125: 41-45.
DiNubile, M., Antin, J., Bressler, S., Stossel, T. and Ferrara, J. (1998). Decreased gelsolin levels are associated with interstitial pneumonia after allogeneic BMT. Blood, 92: 683a.
Filler, G., Bokenkamp, A., Hofmann, W., Le Bricon, T., Martinez-Bru, C. and Grubb, A. (2005). Cystatin C as a marker of GFR-history, indications, and future research. Clinical Biochemistry, 38: 1-8.
Ganz, T. (2003). Hepcidin, a key regulator of iron metabolism and mediator of anemia of inflammation. Blood, 102(3): 783-788.
Gao, Y.L., Yin, H., Luo, J.X., Ouyang, W.Q., Bao, H.M., Guan, G.Q., et al. (2002). Development of an enzyme linked immunosorbent assay for the diagnosis of Theileria sp. infection in sheep. Parasitology Research, 88: 8-10.
Hashemi-Fesharaki, R. (1998). Control of Theileria annulata in Iran. Parasitology Today, 4: 36-40.
Hashemi-Fesharaki, R. (1997). Tick-borne diseases of sheep and goats and their related vectors in Iran. Parasitologia, 39: 115-117.
Herget-Rosenthal, S., Bökenkamp, A. and Hofmann, W. (2007). How to estimate GFR-serum creatinine, serum cystatin C or equations? Clinical Biochemistry, 40: 153-161.
Howard, C.T., McKakpo, U.S., Quakyi, I.A., Bosompem, K.M. and Addison, E.A. (2007). Relationship of hepcidin with parasitemia and anemia among patients with uncomplicated Plasmodium falciparum malaria in Ghana. The American Journal of Tropical Medicine and Hygiene, 77(4): 623-626.
Hui, Q.S., Masaya, Y., Marisan, M. and Yin, H.L. (1999). Gelsolin, a Multifunctional Actin Regulatory Protein. Journal of Biological Chemistry, 274: 33179-33182.
Hu, Y., Li, H., Li, W.H., Meng, H.X., Fan, Y.Z., et al. (2013). The value of decreased plasma gelsolin levels in patients with systemic lupus erythematosus and rheumatoid arthritis in diagnosis and disease activity evaluation. Lupus, 22(14): 1455-1461.
Jensen, A.L.,Bomholt, M. and Moe, L.(2001). Preliminary evaluation of a particle-enhanced turbidimetric immunoassay (PETIA) for the determination of serum cystatin C-like immunereactivity in dogs. Veterinary Clinical Pathology, 30(2): 86-90.
Kanda, J., Mizumoto, C., Kawabata, H., Tsuchida, H., Tomosugi, N. and Matsuo, K. (2008). Serum hepcidin level and erythropoietic activity after hematopoietic stem cell transplantation. Haematologica, 93: 1550-1554.
Kinnaird, J.H., Weir, W., Durrani, Z., Pillai, S.S., Baird, M. and Shiels, B.R. (2013). A bovine lymphosarcoma cell line infected with Theileria annulata exhibits an irreversible reconfiguration of host cell gene expression. PLoS One, 8(6): e66833.
Lassus, J. and Harjola, V.P. (2012). Cystatin C: a step forward in assessing kidney function and cardiovascular risk. Heart Failure Reviews, 17: 251-261.
Lewis, D. and Purnell, R.E. (1981). The piroplasm Theileria ovis detected in sheep in south wales. Veterinary Record, 108(3): 56-57.
Marrocco, C., Rinalducci, S., Mohamadkhani, A., D'Amici, G.M. and Zolla L. (2010). Plasma gelsolin protein: a candidate biomarker for hepatitis B-associated liver cirrhosis identified by proteomic approach. Blood Transfusion, 8(3): 105-112.
Mehta, H.K., Sisodia, R.S. and Misraula, R.S. (1988). Clinical and haematological observations in experimentally induced cases of bovine theileriosis. Indian Journal of Animal Science, 58: 584-587.
Mei, L., Zheng, S., Shengbin, X. and Enyun, S. (2014). Predictive value of serum gelsolin in hepatitis B virus (HBV)-related chronic liver disease. African Journal of Biotechnology, 11(20): 4640-4645.
Miyagawa, Y., Takemura, N. and Hirose, H. (2009). Evaluation of the measurement of serum cystatin C by an enzyme-linked immunosorbent assay for humans as a marker of the glomerular filtration rate in dogs. Journal of Veterinary Medicine Science, 71: 1169-1176.
Oge, H., Doganay, A., Oge, S. and Yildirim, A. (2003). Prevalence and distribution of Dirofilaria immitis in domestic dogs from Ankara and vicinity in Turkey. Dtsch Tierarztl Wochenschr, 110: 69-72.
Park, C.H., Valore, E.V., Waring, A.J. and Ganz, T. (2001). Hepcidin, a urinary antimicrobial peptide synthesized in liver. Journal of Biological Chemistry, 276: 7806-7810.
Pasa, S., Bayramli, G., Atasoy, A., Karul, A., Ertug, S. and Ozensoy Toz, S. (2009). Evaluation of serum cystatin-C in dogs with visceral leishmaniasis. Veterinary Research Communications, 33(6): 529-534.
Pekmezci, D., Guzel, M., Yildirim, A., Ciftci, G., Pekmezci, G.Z., Tutuncu, M., et al. (2015a). Evaluation of serum cystatin-C concentrations in dogs infected with Dirofilaria immitis. Ankara Universitesi Veteriner Fakultesi Dergisi, 62(4): 303-306.
Pekmezci, D., Ural, K., Aysul, N., Guzel, M. and Ciftci, G. (2015b). Assessment of renal function using canine cystatin- C levels in canine babesiosis and ehrlichiosis. Acta Veterinaria-Beograd, 65(1): 56-65.
Randers, E. and Erlandsen, E.J. (1999). Serum cystatin C as an endogenous marker of the renal function – A Review. Clinical Chemistry Laboratory Medicine, 37: 389-395.
Rayula, V. and Hafeez, M.D. (1995). Haematological values in cattle infected with Theileria annulata. Indian Journal of Animal Science, 56: 1202-1203.
Singh, A., Singh, J., Grewal, A.S. and Brar, R.S. (2001). Studies on some blood parameters of crossbred calves with experimental Theileria annulata infections. Veterinary Research Communication, 25: 289-300.
Smith, D.B., Janmey, P.A., Sherwood, J.A., Howard, R.J. and Lind, S.E. (1988). Decreased plasma gelsolin levels in patients with Plasmodium falciparum malaria: a consequence of hemolysis? Blood, 72(1): 214-218.
Spottiswoode, N., Patrick, E.D. and Drakesmith, H. (2014). Iron, anemia and hepcidin in malaria. Front Pharmacology, 5: 125.
Soulsby, E.J.L. (1982). Helminths, Arthropods and Protozoa of Domesticated Animals. 7th ed., London: ELBS, Bailliere Tindall, pp: 728-741.
Van Velthuysen, M.L.F. and Florquin, S. (2000). Glomerulopathy associated with parasitic infections." Clinical Microbiology Reviews, 13(1): 55-66.
Villa, P., Jiménez, M., Soriano, M.C., Manzanares, J. and Casasnovas, P. (2005). Serum cystatin C concentration as a marker of acute renal dysfunction in critically ill patients. Critical Care, 9: 139-143.
Yilmaz, E., Ayarci, A.O., Sigirli, D., Torlar, M.O., Budak, F. and Goral, G. (2014). increased serum hepcidin levels in brucellosis. Clinical Laboratory, 60(11): 1837-1843.
Young, C.L., Feierstein, A. and Southwick, F.S. (1994). Calcium regulation of actin filament capping and monomer binding by macrophage capping protein. Journal of Biological Chemistry, 269(19): 13997-14002.
Zhao, D.Q., Wang, K., Zhang, H.D. and Li, Y.J. (2013). Significant reduction of plasma gelsolin levels in patients with intracerebral hemorrhage. Clinica Chimica Acta, 415: 202-206.
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