جستجوی رئوویروس‌ها در موارد پیش‌معده التهابی جوجه‌های گوشتی استان اصفهان به روش مولکولی RT-PCR و ارزیابی پاتولوژیکی ضایعات مربوطه
الموضوعات :
سارا کریمی
1
,
مجید غلامی آهنگران
2
1 - دانش‌آموخته دکترای حرفه‌ای دامپزشکی، دانشکده دامپزشکی، واحد شهرکرد، دانشگاه آزاد اسلامی، شهرکرد، ایران.
2 - دانشیار علوم درمانگاهی، دانشکده دامپزشکی، واحد شهرکرد، دانشگاه آزاد اسلامی، شهرکرد، ایران.
تاريخ الإرسال : 15 الثلاثاء , ربيع الأول, 1444
تاريخ التأكيد : 28 الأحد , رجب, 1444
تاريخ الإصدار : 28 السبت , جمادى الثانية, 1444
الکلمات المفتاحية:
هیستوپاتولوژی,
جوجه گوشتی,
رئوویروس,
التهاب پیش‌معده,
ردیابی مولکولی,
ملخص المقالة :
التهاب پیش‌معده با بزرگ‌شدگی این ارگان مشخص می‌شود و عوامل متعدد عفونی و غیر عفونی در ایجاد آن نقش دارند و از جمله این عوامل رئوویروس‌ها، هستند. براساس شواهد درمانگاهی و گزارشاتی از شیوع رئوویروس‌ها در مزارع پرورش طیور در ایران، می‌توان بزرگ شدگی و التهاب پیش‌معده در جوجه‌های گوشتی را با عفونت‌های رئوویروسی مرتبط دانست. در بررسی حاضر، به منظور شناسایی و ردیابی رئوویروس ها در بافت پیش‌معده، ژن S1 به روش RT-PCR (Reverse Transcription Polymerase Chain Reaction) مورد تکثیر قرار گرفت. براساس یافته ها، از 75 نمونه پیش‌معده جمع‌آوری شده از 15 فارم جوجه گوشتی استان اصفهان با علایم بزرگی و التهاب پیش‌معده، 46 نمونه (33/61 درصد نمونه ها) و 10 فارم (66/66 درصد فارم های بررسی شده)، بر اساس تکثیر قطعه 532 جفت بازی مربوط به ژن S1 ، از نظر حضور رئوویروس ها مثبت ارزیابی شدند. همچنین پیش‌معده‌های جمع آوری شده، همگی از لحاظ ماکروسکوپی بزرگ‌تر از حالت عادی بوده و ضخامت بیشتری داشتند. ناحیه اتصال به سنگدان هم شل و فاقد قوام کافی بود. علاوه بر آن، بافت مخاطی پیش‌معده لزج و حاوی ترشحات زرد رنگ بود. در تمامی مقاطع هیستوپاتولوژی مربوط به نمونه‌های مثبت از لحاظ رئوویروس نیز، نکروز به همراه سلول‌های التهابی در غدد سطحی و عمقی پیش‌معده قابل مشاهده بود. به نظر می‌رسد آلودگی با رئوویروس ها در جوجه‌های گوشتی استان اصفهان وجود دارد و این آلودگی می‌تواند در ایجاد عارضه التهاب و بزرگ‌شدگی پیش‌معده نقش داشته باشد. لذا اقدامات لازم برای کنترل و پیشگیری از این آلودگی در جوجه‌های گوشتی ضروری است.
المصادر:
Allawe, A.B., Abbas, A.A. and Taha, Z.H. (2017). Detection of transmissible viral proventriculitis in Iraq. Journal of Entomology and Zoology Studies, 5 (3): 974-978.
Asadi, J., Gholami-Ahangaran, M. and Momtaz, H. (2018). Molecular detection of Ornithobacterium rhinotracheale in quails of Isfahan province. Veterinary Clinical Pathology, 12(45): 1-7. [In Persian]
Al-Ebshahy, E., Mohamed, S. and Abas, O. (2020). First report of seroprevalence and genetic characterization of avian orthoreovirus in Egypt. Journal of Tropical Animal Health and Productio, 52 (2): 1049-1054.
Bancroft, J.D. and Gamble, M. (2008). Theory and practice of histological techniques. 6th ed., Churchill Livingstone: Elsevier Health Sciences, pp: 100-110.
Benavente, J. and Martínez-Costas, J. (2007). Avian reovirus: structure and biology. Virus Research, 123(2): 105-119.
Cătană, N., Coman, A. and Fodor, I. (2012). Research on the role of avian reovirus in transmissible viral etiology proventriculitis. Lucrari Stiintifice-Universitatea de Stiinte Agricole a Banatului Timisoara, Medicina Veterinara, 45(3): 23-26.
Dormitorio, T.V, Giambrone, J.J. and Hoerr, F.J. (2007). Transmissible proventriculitis in broilers. Avian Pathology, 36(2): 87-91.
Li, G., Yuan, S., He, M., Zhao, M., Hao, X., Song, M., et al. (2018). Emergence of gyrovirus 3 in commercial broiler chickens with transmissible viral proventriculitis. The 16th World Veterinary Poultry Association Transboundary and Emerging Diseases, 65(5): 1170-1174.
Gholami-Ahangaran, M., Moghtadaiee, E. and Ahmadi, A. (2019). Molecular detection of Marek,s virus in backyard fowl with nodular lesions in visceral organs. Veterinary Clinical Pathology, 13(50): 151-161. [In Persian]
Grau-Roma, L., Schock, A., Nofrarías, M., Ali Wali, N., de Fraga, A.P., Garcia-Rueda, C., et al. (2020). Retrospective study on transmissible viral proventriculitis and chicken proventricular necrosis virus (CPNV) in the UK. Avian Pathology, 49(1): 99-105.
Guy, J.S. (1998). Virus infections of the gastrointestinal tract of poultry. Poultry Science, 77(8): 1166-1175.
Hafner, S. and Guy, J.S. (2013). Proventriculitis and proventricular dilatation of broiler chickens. In: Diseases of Poultry. Swayne, D.E., Glisson, J.R., McDouglald, L.R., Nolan, L.K., Suarez, D.L. and Nair, V.L. editors. 13th ed., Ames, IA: Wiley-Blackwell Publishing, pp: 1328-1332.
Hauck, R., Stoute, S., Senties-Cue, C.G., Guy, J.S. and Shivaprasad, H. L. (2020). A retrospective study of transmissible viral proventriculitis in broiler chickens in California: 2000–18. Avian Diseases, 64(4): 525-531.
Hedayati, M., Shojadoust, B. and Peyghambari, M. (2012). Identification of reoviruses causing tenosynovitis in birds from Iranian mother hen flocks by RT-PCR and RFLP methods. Iranian Journal of Veterinary Medicine, 7(2): 135-142.
Jeong, J.K., Jeong, H., An, E. and Han, S. (2019). The prevalence of avian reovirus infection in poultry farms of Jeonbuk province, Korea. Korean Journal of Veterinary Service, 42(4): 237-243.
Jones, R.C. and Georgiou, K. (1984). Reovirus‐induced tenosynovitis in chickens the influence of age at infection. Avian Pathology, 13(3): 441-457.
Kim, H.R., Yoon, S.J., Lee, H.S. and Kwon, Y.K. (2015). Identification of a picornavirus from chickens with transmissible viral proventriculitis using metagenomic analysis. Archives of Virology, 160(3): 701-709.
Kouwenhoven, B., Vertommen, M. and Goren, E. (1988). Investigations into the role of reovirus in the malabsorption syndrome. Avian Pathology, 17(4): 879-892.
Kutkat, M.A., Hoda, M.A., Khalil, S.A., El-Fatah, M.A. and Torky, H.A. (2010). Studies on proventriculitis in broilers with molecular characterization of its viral causes. Journal of American Science, 6(9): 582-592.
Lenz, S.D., Hoerr, F.J., Ellis, A.C., Toivio-Kinnucan, M.A. and Yu, M. (1998). Gastrointestinal pathogenicity of adenoviruses and reoviruses isolated from broiler chickens in Alabama. Journal of Veterinary Diagnostic Investigation, 10(2): 145-151.
Marusak, R.A., West, M.A., Davis, J.F., Fletcher, O.J. and Guy, J.S. (2012). Transmissible viral proventriculitis identified in broiler breeder and layer hens. Avian Diseases, 56(4): 757-759.
Mohamed, S., Al-Ebshahy, E.M., Khalil, S.A., AbdelHady, H.A. and Elbestawy, A.R. (2019). Molecular identification and pathogenicity assessment of avian reovirus in Egypt. Alexandria Journal for Veterinary Sciences, 63(1): 100-110.
Pantin-Jackwood, M.J., Brown, T.P. and Huff, G.R. (2004). Proventriculitis in broiler chickens: immunohistochemical characterization of the lymphocytes infiltrating the proventricular glands. Veterinary Pathology, 41(6): 641-648.
Robertson, M.D. and Wilcox, G.E. (1986). Avian reovirus. Veterinary Bulletin, 56 (4): 154-174.
Sellers, H.S. (2017). Current limitations in control of viral arthritis and tenosynovitis caused by avian reoviruses in commercial poultry. Veterinary Microbiology, 206: 152-156.
Sellers, H.S. (2022). Avian reoviruses from clinical cases of tenosynovitis: An overview of diagnostic approaches and 10-year review of isolations and genetic characterization. Avian Diseases, 66(4): 420-426.
Shabani, A.A., Gholami-Ahangaran, M. and Momtaz, H. (2017). Molecular detection of Ornithbacterium rhinotracheale and Newcastle disease virus in ostriches of Isfahan province. Veterinary Clinical Pathology, 11(42): 97-105. [In Persian]
Souza, S.O., De Carli, S., Lunge, V.R., Ikuta, N., Canal, C.W., Pavarini, S.P., et al. (2018). Pathological and molecular findings of avian reoviruses from clinical cases of tenosynovitis in poultry flocks from Brazil. Poultry Science, 97(10): 3550-3555.
Troxler, S., Rigomier, P., Bilic, I., Liebhart, D., Prokofieva, I., Robineau, B., et al. (2013). Identification of a new reovirus causing substantial losses in broiler production in France, despite routine vaccination of breeders. Veterinary Record, 172(21): 556-556.
Xie, Z., Fadl, A.A., Girshick, T. and Khan, M.I. (1997). Amplification of avian reovirus RNA using the reverse transcriptase-polymerase chain reaction. Avian Diseases, 18(2): 654-660.
Yan, T., Li, G., Zhou, D., Hu, L., Hao, X., Li, R. and Cheng, Z. (2022). Long read sequencing revealed proventricular virome of broiler chicken with transmission viral proventriculitis. BMC Veterinary Research, 18(1): 1-7.
_||_
Allawe, A.B., Abbas, A.A. and Taha, Z.H. (2017). Detection of transmissible viral proventriculitis in Iraq. Journal of Entomology and Zoology Studies, 5 (3): 974-978.
Asadi, J., Gholami-Ahangaran, M. and Momtaz, H. (2018). Molecular detection of Ornithobacterium rhinotracheale in quails of Isfahan province. Veterinary Clinical Pathology, 12(45): 1-7. [In Persian]
Al-Ebshahy, E., Mohamed, S. and Abas, O. (2020). First report of seroprevalence and genetic characterization of avian orthoreovirus in Egypt. Journal of Tropical Animal Health and Productio, 52 (2): 1049-1054.
Bancroft, J.D. and Gamble, M. (2008). Theory and practice of histological techniques. 6th ed., Churchill Livingstone: Elsevier Health Sciences, pp: 100-110.
Benavente, J. and Martínez-Costas, J. (2007). Avian reovirus: structure and biology. Virus Research, 123(2): 105-119.
Cătană, N., Coman, A. and Fodor, I. (2012). Research on the role of avian reovirus in transmissible viral etiology proventriculitis. Lucrari Stiintifice-Universitatea de Stiinte Agricole a Banatului Timisoara, Medicina Veterinara, 45(3): 23-26.
Dormitorio, T.V, Giambrone, J.J. and Hoerr, F.J. (2007). Transmissible proventriculitis in broilers. Avian Pathology, 36(2): 87-91.
Li, G., Yuan, S., He, M., Zhao, M., Hao, X., Song, M., et al. (2018). Emergence of gyrovirus 3 in commercial broiler chickens with transmissible viral proventriculitis. The 16th World Veterinary Poultry Association Transboundary and Emerging Diseases, 65(5): 1170-1174.
Gholami-Ahangaran, M., Moghtadaiee, E. and Ahmadi, A. (2019). Molecular detection of Marek,s virus in backyard fowl with nodular lesions in visceral organs. Veterinary Clinical Pathology, 13(50): 151-161. [In Persian]
Grau-Roma, L., Schock, A., Nofrarías, M., Ali Wali, N., de Fraga, A.P., Garcia-Rueda, C., et al. (2020). Retrospective study on transmissible viral proventriculitis and chicken proventricular necrosis virus (CPNV) in the UK. Avian Pathology, 49(1): 99-105.
Guy, J.S. (1998). Virus infections of the gastrointestinal tract of poultry. Poultry Science, 77(8): 1166-1175.
Hafner, S. and Guy, J.S. (2013). Proventriculitis and proventricular dilatation of broiler chickens. In: Diseases of Poultry. Swayne, D.E., Glisson, J.R., McDouglald, L.R., Nolan, L.K., Suarez, D.L. and Nair, V.L. editors. 13th ed., Ames, IA: Wiley-Blackwell Publishing, pp: 1328-1332.
Hauck, R., Stoute, S., Senties-Cue, C.G., Guy, J.S. and Shivaprasad, H. L. (2020). A retrospective study of transmissible viral proventriculitis in broiler chickens in California: 2000–18. Avian Diseases, 64(4): 525-531.
Hedayati, M., Shojadoust, B. and Peyghambari, M. (2012). Identification of reoviruses causing tenosynovitis in birds from Iranian mother hen flocks by RT-PCR and RFLP methods. Iranian Journal of Veterinary Medicine, 7(2): 135-142.
Jeong, J.K., Jeong, H., An, E. and Han, S. (2019). The prevalence of avian reovirus infection in poultry farms of Jeonbuk province, Korea. Korean Journal of Veterinary Service, 42(4): 237-243.
Jones, R.C. and Georgiou, K. (1984). Reovirus‐induced tenosynovitis in chickens the influence of age at infection. Avian Pathology, 13(3): 441-457.
Kim, H.R., Yoon, S.J., Lee, H.S. and Kwon, Y.K. (2015). Identification of a picornavirus from chickens with transmissible viral proventriculitis using metagenomic analysis. Archives of Virology, 160(3): 701-709.
Kouwenhoven, B., Vertommen, M. and Goren, E. (1988). Investigations into the role of reovirus in the malabsorption syndrome. Avian Pathology, 17(4): 879-892.
Kutkat, M.A., Hoda, M.A., Khalil, S.A., El-Fatah, M.A. and Torky, H.A. (2010). Studies on proventriculitis in broilers with molecular characterization of its viral causes. Journal of American Science, 6(9): 582-592.
Lenz, S.D., Hoerr, F.J., Ellis, A.C., Toivio-Kinnucan, M.A. and Yu, M. (1998). Gastrointestinal pathogenicity of adenoviruses and reoviruses isolated from broiler chickens in Alabama. Journal of Veterinary Diagnostic Investigation, 10(2): 145-151.
Marusak, R.A., West, M.A., Davis, J.F., Fletcher, O.J. and Guy, J.S. (2012). Transmissible viral proventriculitis identified in broiler breeder and layer hens. Avian Diseases, 56(4): 757-759.
Mohamed, S., Al-Ebshahy, E.M., Khalil, S.A., AbdelHady, H.A. and Elbestawy, A.R. (2019). Molecular identification and pathogenicity assessment of avian reovirus in Egypt. Alexandria Journal for Veterinary Sciences, 63(1): 100-110.
Pantin-Jackwood, M.J., Brown, T.P. and Huff, G.R. (2004). Proventriculitis in broiler chickens: immunohistochemical characterization of the lymphocytes infiltrating the proventricular glands. Veterinary Pathology, 41(6): 641-648.
Robertson, M.D. and Wilcox, G.E. (1986). Avian reovirus. Veterinary Bulletin, 56 (4): 154-174.
Sellers, H.S. (2017). Current limitations in control of viral arthritis and tenosynovitis caused by avian reoviruses in commercial poultry. Veterinary Microbiology, 206: 152-156.
Sellers, H.S. (2022). Avian reoviruses from clinical cases of tenosynovitis: An overview of diagnostic approaches and 10-year review of isolations and genetic characterization. Avian Diseases, 66(4): 420-426.
Shabani, A.A., Gholami-Ahangaran, M. and Momtaz, H. (2017). Molecular detection of Ornithbacterium rhinotracheale and Newcastle disease virus in ostriches of Isfahan province. Veterinary Clinical Pathology, 11(42): 97-105. [In Persian]
Souza, S.O., De Carli, S., Lunge, V.R., Ikuta, N., Canal, C.W., Pavarini, S.P., et al. (2018). Pathological and molecular findings of avian reoviruses from clinical cases of tenosynovitis in poultry flocks from Brazil. Poultry Science, 97(10): 3550-3555.
Troxler, S., Rigomier, P., Bilic, I., Liebhart, D., Prokofieva, I., Robineau, B., et al. (2013). Identification of a new reovirus causing substantial losses in broiler production in France, despite routine vaccination of breeders. Veterinary Record, 172(21): 556-556.
Xie, Z., Fadl, A.A., Girshick, T. and Khan, M.I. (1997). Amplification of avian reovirus RNA using the reverse transcriptase-polymerase chain reaction. Avian Diseases, 18(2): 654-660.
Yan, T., Li, G., Zhou, D., Hu, L., Hao, X., Li, R. and Cheng, Z. (2022). Long read sequencing revealed proventricular virome of broiler chicken with transmission viral proventriculitis. BMC Veterinary Research, 18(1): 1-7.
