ارتباط برخی از متابولیت‌های خون و بیان ژن فاکتور هسته‌ای رونویسی کاپا B (Nuclear factor kappa B) با کتوز تحت‌بالینی در گاوهای هلشتاین در طول دوره انتقال
الموضوعات :
سعیده مرادی
1
(دانشجوی دکترای گروه علوم دامی، دانشکده کشاورزی، دانشگاه تبریز، تبریز، ایران.)
غلامعلی مقدم
2
(استاد گروه علوم دامی، دانشکده کشاورزی، دانشگاه تبریز، تبریز، ایران.)
صمد لطف‌اله‌زاده
3
(دانشیار گروه علوم درمانگاهی، دانشکده دامپزشکی، دانشگاه تهران، تهران، ایران.)
رضی‌اله جعفری
4
(دانشیار گروه علوم درمانگاهی، دانشکده دامپزشکی، دانشگاه تبریز، تبریز، ایران.)
سید‌عباس رافت
5
(استاد گروه علوم دامی، دانشکده کشاورزی، دانشگاه تبریز، تبریز، ایران.)
تاريخ الإرسال : 19 الأربعاء , رمضان, 1443
تاريخ التأكيد : 15 السبت , محرم, 1444
تاريخ الإصدار : 25 الثلاثاء , محرم, 1444
الکلمات المفتاحية:
گاو هلشتاین,
متابولیت‌های خون,
کتوز تحت‌بالینی,
استرس‌اکسیداتیو,
فاکتور هسته‌ای رونویسی کاپا B,
ملخص المقالة :
کتوز تحت بالینی اثرات اقتصادی بر صنعت گاو شیری تحمیل کرده و گاوها با افزایش استرس اکسیداتیو، مستعد ابتلا به این نوع کتوز می شوند. از آنجایی‌که فاکتور هسته‌ای رونویسی کاپا B (NF-κB Nuclear factor kappa B;) به‌عنوان فاکتور پاسخ سلولی به انواع استرس با منشأ درونی می‌باشد، در تحقیق حاضر، ارتباط برخی از متابولیت‌های خون و بیان ژن NF-κB با کتوز تحت بالینی در گاوهای هلشتاین در طول دوره انتقال، در یک گاوداری 2000 رأسی با 900 رأس گاو دوشا بررسی گردید. بدین منظور 5 میلی‌لیتر خون از 100 رأس گاو شکم چهارم و شکم پنجم زایش، یک هفته قبل و نیز یک هفته بعد از زایش، از ورید دمی اخذ شد. بر اساس یافته ها، در هفته قبل از زایش همبستگی مثبتی بین اسیدهای چرب غیراستریفیه (NEFAs Non-esterified fatty acids;) و بتاهیدروکسی بوتیرات (Beta-hydroxybutyrate; BHBA)، مالون دی‌آلدئید، گاما گلوتامیل ترانسفراز و NF-κB و نیز همبستگی منفی با لیپوپروتئین با تراکم بالا مشاهده شد. در هفته بعد از زایش هم همبستگی مثبتی بین BHBA، NEFA، مالون دی‌آلدئید، گاما گلوتامیل ترانسفراز، آلکالین فسفاتاز و NF-κB و نیز همبستگی منفی با لیپوپروتئین با تراکم بالا مشاهده شد. نقطه برش mM/L 2/1BHBA≤ برای گاوهای سالم و mM/L 2/1BHBA> برای گاوهای مبتلا به کتوز تحت بالینی در بعد از زایش تعیین شد. همچنین در گاوهای درگیر، مقادیرNEFA ،BHBA ، گاماگلوتامیل ترانسفراز (0001/ (p< و آلکالین فسفاتاز (03/0 p=) افزایش و لیپوپروتئین با تراکم بالا و NF-κB (0001/p<) کاهش داشت. نتایج تحقیق حاضر مشخص کرد که افزایش NEFAs و BHBA با فعال‌سازی مسیر NF-κB و استرس اکسیداتیو، دام را مستعد ابتلا به بیماری کتوز می‌کند.
المصادر:
Adias, T.C., Egerton, E. and Erhabor, O. (2013). Evaluation of coagulation parameters and liver enzymes among alcohol drinkers in Port Harcourt, Nigeria. International Journal of General Medicine, 6(1): 489-94.
Amouoghli Tabrizi, B., Safi, S., Asri Rezaee, S. and Abdie Nojamehr. (2007). Study of the levels of betahydroxy butyrate, glucose, protein and albumin in Holstein cows with subclinical ketosis. Journal of Veterinary Clinical Pathology, 2(2): 125-132. [In Persian]
Asri Rezaee, S., Amouoghli Tabrizi, B. and Saber Marouf, B. (2012). Evaluation of the levels of Leptin, Beta hydroxyl butyrate, Glucose, Cholesterol and Triglyceride in serum of Holstein cows with sub clinical ketosis. Journal of Veterinary Clinical pathology, 6(3): 1647-1656. [In Persian]
Baker, R.G., Hayden, M.S. and Ghosh, S. (2011). NF-κB, inflammation, and metabolic disease. Cell Metabolism, 13(1): 11-22.
Bernabucci, U., Ronchi, B., Lacetera, N. and Nardone, A. (2005). Influence of body condition score on relationships between metabolic status and oxidative stress in periparturient dairy cows. Journal of Dairy Science, 88(6): 2017-2026.
Chapinal, N., Carson, M., Duffield, T.F., Capel, M., Godden, S., Overton, M., et al. (2011). The association of serum metabolites with clinical disease during the transition period. Journal of Dairy Science, 94(10): 897-903.
Contreras, G.A., Raphael, W., Mattmiller, S.A., Gandy, J. and Sordilo, L.M. (2012). Nonesterified fatty acids modify inflammatory response and eicosanoid biosynthesis in bovine endothelial cells. Journal of Dairy Science, 95(9): 5011-5023.
Czaja, M.J. (2007). Cell signaling in oxidative stress-induced liver injury. In Seminars in Liver Disease, 27(4) : 378-389.
Dann, H.M. and Drackley, J.K. (2005). Carnitine palmitoyltransferaseI in liver of periparturient dairy cows: effects of prepartum intake, postpartum induction of ketosis, and periparturient disorders. Journal of Dairy Science, 88(11): 3851-3859.
Denk, A., Wirth, T. and Baumann, B. (2000). NF-κB transcription factors: critical regulators of hematopoiesis and neuronal survival. Cytokine and Growth Factor Reviews, 11(4): 303-320.
Dorrington, M.G. and Fraser, I.D. (2019). NF-κB signaling in macrophages: dynamics, crosstalk, and signal integration. Frontiers in Immunology, 10(1): 705-708.
Drackley, J. K. (1999). Biology of dairy cows during the transition period: The final frontier?. Journal of Dairy Science, 82(11): 2259-2273.
Du, X., Chen, L., Huang, D., Peng, Z., Zhao, C., Zhang, Y., et al. (2017). Elevated apoptosis in the liver of dairy cows with ketosis. Cellular Physiology and Biochemistry, 43(2): 568-578.
El-Bahr, S.M. and El-Deeb, W.M. (2017). Oxidative stress and cardiac biomarkers in lambs affected with enzootic ataxia: the diagnostic and prognostic significance. Veterinarski Arhiv, 87(3): 259-271.
El-Deeb, W.M. and Younis, E.E. (2009). Clinical and biochemical studies on Theileria annulata in Egyptian buffaloes (Bubalus bubalis) with particular orientation to oxidative stress and ketosis relationship. Veterinary Parasitology, 164(2-4): 301-305.
Feldstein, A.E., Werneburg, N.W., Canbay, A., Guicciardi, M.E., Bronk, S.F., Rydzewski, R., et al. (2004). Free fatty acids promote hepatic lipotoxicity by stimulating TNFα expression via a lysosomal pathway. Hepatology, 40(1): 185-194.
Fiore, F., Spissu, N., Sechi, S. and Coccom, R. (2019). Evaluation of oxidative stress in dairy cows with left displacement of abomasum. Animal, 9(11): 966.
Galvão, K.N., Flaminio, M.J.B.F., Brittin, S.B., Sper, R., Fraga, M., Caixeta, L., et al. (2010). Association between uterine disease and indicators of neutrophil and systemic energy status in lactating Holstein cows. Journal of Dairy Science, 93(7): 2926-2937.
González, F.D., Muiño, R., Pereira, V., Campos, R. and Benedito, J.L. (2011). Relationship among blood indicators of lipomobilization and hepatic function during early lactation in high-yielding dairy cows. Journal of Veterinary Science, 12(3): 251-255.
Grummer, R.R. (1995). Impact of changes in organic nutrient metabolism on feeding the transition dairy cow. Journal of Animal Science, 73(9): 2820-2833.
Hammon, D., Evjen, I.M., Dhiman, T.R., Goff, J.P. and Walters, L.J. (2006). Neutrophil function and energy status in Holstein cows with uterine health disorders. Veterinary Immunology and Immunopathology, 113(1-2): 21-29.
Hayden, M.S. and Ghosh, S. (2008). Shared principles in NF-κB signaling. Cell, 132(3): 344-362.
Hayden, M.S., West, A.P. and Ghosh, S. (2006). NF-κB and the immune response. Oncogene, 25(51): 6758-6780.
Herdt, T.H. (2000). Ruminant adaptation to negative energy balance: Influences on the etiology of ketosis and fatty liver. Veterinary Clinics of North America: Food Animal Practice, 16(2): 215-230.
Hybertson, B.M., Gao, B., Bose, S.K. and McCord, J.M. (2011). Oxidative stress in health and disease: the therapeutic potential of Nrf2 activation. Molecular Aspects of Medicine, 32(4-6): 234-246.
Ji, L.L., Gomez-Cabera, M.C. and Vina, J. (2006). Exercise and hormesis. Annals of the New York Academy of Sciences, 1067(1): 425-435.
LeBlanc, S.J., Osawa, T. and Dubuc, J. (2011). Reproductive tract defense and disease in postpartum dairy cows. Theriogenology, 76(9): 1610-1618.
Leopold, J. A. and Loscalzo, J. (2009). Oxidative risk for atherothrombotic cardiovascular disease. Free Radical Biology and Medicine, 47(12): 1673-1706.
Li, X., Chen, H., Lei, L., Liu, J., Guan, Y., Liu, Z., et al. (2013). Non-esterified fatty acids activate the AMP-activated protein kinase signaling pathway to regulate lipid metabolism in bovine hepatocytes. Cell Biochemistry and Biophysics, 67(3): 1157-1169.
Li, Y., Ding, H.Y., Wang, X.C., Feng, S.B., Li, X.B., Wang, Z., et al. (2016). An association between the level of oxidative stress and the concentrations of NEFA and BHBA in the plasma of ketotic dairy cows. Journal of Animal Physiology and Animal Nutrition, 100(5): 844-851.
Liguori, I., Russo, G., Curcio, F., Bulli, G., Aran, L., Della-Morte, D., et al. (2018). Oxidative stress, aging, and diseases. Clinical Interventions in Aging, 13: 757-772.
Liu, T., Zhang, L., Joo, D. and Sun, S.C. (2017). NF-κB signaling in inflammation. Signal Transduction and Targeted Therapy, 2: 17023.
Mezzetti, M., Cattaneo, L., Maria Passamonti, M., Lopreiato, V., Minuti, A. and Trevisi, E. (2021). The Transition Period Updated: A Review of the New Insights into the Adaptation of Dairy Cows to the New Lactation. Dairy, 2(4): 617-636.
Morgan, M.J. and Liu, Z.G. (2011). Crosstalk of reactive oxygen species and NF-κB signaling. Cell Research, 21(1): 103-115.
Nelson, D.L., Cox, M.M. and Lehninger, A.L. (2005). Principles of biochemistry. 4th ed., USA: New York, Freeman, pp: 600-622.
Niu, Q., Cao, M., Chen, S., Zhao,Y., Li, C. and Zhou, X. (2019). Effective and economical column- based method for RNA isolation from animal cells. Biotechnology Letters, 41(8-9): 915-920.
Ospina, P.A., Nydam, D.V., Stokol, T. and Overton, T.R. (2010). Evaluation of nonesterified fatty acids and beta-hydroxybutyrate in transition dairy cattle in the northeastern United States: critical thresholds for prediction of clinical diseases. Journal of Dairy Science, 93(4): 546-554.
Pedernera, M., Celi, P., García, S.C., Salvin, E.H., Barchia, I. and Fulkerson, W.J. (2010). Effect of diet, energy balance and milk production on oxidative stress in early-lactating dairy cows grazing pasture. The Veterinary Journal, 186(3): 352-357.
Pfaffl, M.W. (2001). A new mathematical model for relative quantification in real-time RT–PCR. Nucleic acids Research, 29(9): 45-49.
Puchalska, P. and Crawford, P.A. (2017). Multi-dimensional roles of ketone bodies in fuel metabolism, signaling, and therapeutics. Cell Metabolism, 25(2): 262-284.
Puppel, K., Gołębiewski, M., Solarczyk, P., Grodkowski, G., Slósarz, J., Kunowska-Slósarz, M., et al. (2019). The relationship between plasma β-hydroxybutyric acid and conjugated linoleic acid in milk as a biomarker for early diagnosis of ketosis in postpartum Polish Holstein-Friesian cows. BMC Veterinary Research, 15(1): 367.
Romagnoli, M., Gomez-Cabrera, M.C., Perrelli, M.G., Biasi, F., Pallardo, V.F., Sastre, J., et al. (2010). Xanthine oxidase-induced oxidative stress causes activation of NF-κB and inflammation in the liver of type I diabetic rats. Free Radical Biology and Medicine, 49(2): 171-177.
Sadeghi-nasab, A., Hassanpour, A., Sabaghsaray, H. and Amiri-Sadeghan, S. (2011). Assessment of thyroid hormones, insulin and magnesium in dairy cattle with subclinical ketosis. Journal of Veterinary Clinical Pathology, 5(2): 1149-1159. [In Persian]
Senoh, T., Oikawa, S., Nakada, K., Tagami, T. and Iwasaki, T. (2019). Increased serum malondialdehyde concentration in cows with subclinical ketosis. Journal of Veterinary Medical Science. 81: 817-820.
Shi, X., Li, D., Deng, Q., Li, Y., Sun, G., Yuan, X., et al. (2015). NEFAs activate the oxidative stress-mediated NF-κB signaling pathway to induce inflammatory response in calf hepatocytes. The Journal of Steroid Biochemistry and Molecular Biology, 145(8): 103-112.
Sordillo, L.M. and Aitken, S.L. (2009). Impact of oxidative stress on the health and immune function of dairy cattle. Veterinary Immunology and Immunopathology, 128(1-3): 104-109.
Sordillo, L.M. and Raphael, W. (2013). Significance of metabolic stress, lipid mobilization,and inflammation on transition cow disorders. Veterinary Clinics of North America: Food Animal Practice, 29(2): 267-278.
Stokol, T. and Nydam, D.V. (2006). Effect of hemolysis on nonesterified fatty acid and beta-hydroxybutyrate concentrations in bovine blood. Journal of Veterinary Diagnostic Investigation, 18(5): 466-469.
Suriyasathaporn, W.S., Heuer, C.H., Noordhuizen-Stassen, E.N. and Schukken, Y.H.S. (2000). Hyperketonemia and the impairment of udder defense: a review. Veterinary Research, 31(4): 397-412.
Turk, R., Juretić, D., Gereš, D., Svetina, A., Turk, N. and Flegar-Mestric, Z. (2008). Influence of oxidative stress and metabolic adaptation on PON1 activity and MDA level in transition dairy cows. Animal Reproduction Science, 108(1-2): 98-106.
Turk, R., Juretić, D., Gereš, D., Turk, N., Simeon-Rudolf, V., Rekić, B., et al. (2005). Oxidative stress in dairy cows–serum paraoxonase activity related to hepatomegaly. Croatica Chemica Acta, 78(3): 375-378.
Wang, D., Yu, D., Zhao, C., Xia, C., Xu, C. and Wu, L. (2021). Subclinical ketosis risk prediction in dairy cows based on prepartum metabolic indices. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 73(1): 11-17.
Wankhade, P.R., Manimaran, A., Kumaresan, A., Jeyakumar, S., Ramesha, K.P., Sejian, V., et al. (2017). Metabolic and immunological changes in transition dairy cows: A review. Veterinary World, 10(11): 1367-1377.
Weisberg, S.P., Hunter, D., Huber, R., Lemieux, J., Slaymaker, S., Vaddi, K., et al. (2006). CCR2 modulates inflammatory and metabolic effects of high-fat feeding. Journal of Clinical Investigation, 116(1): 115-124.
Wree, A., Schlattjan, M., Bechmann, L.P., Claudel, T., Sowa, J.P., Stojakovic, T., et al. (2014). Adipocyte cell size, free fatty acids and apolipoproteins are associated with non-alcoholic liver injury progression in severely obese patients. Metabolism: Clinical and Experimental, 63(12): 1542-1552.
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Adias, T.C., Egerton, E. and Erhabor, O. (2013). Evaluation of coagulation parameters and liver enzymes among alcohol drinkers in Port Harcourt, Nigeria. International Journal of General Medicine, 6(1): 489-94.
Amouoghli Tabrizi, B., Safi, S., Asri Rezaee, S. and Abdie Nojamehr. (2007). Study of the levels of betahydroxy butyrate, glucose, protein and albumin in Holstein cows with subclinical ketosis. Journal of Veterinary Clinical Pathology, 2(2): 125-132. [In Persian]
Asri Rezaee, S., Amouoghli Tabrizi, B. and Saber Marouf, B. (2012). Evaluation of the levels of Leptin, Beta hydroxyl butyrate, Glucose, Cholesterol and Triglyceride in serum of Holstein cows with sub clinical ketosis. Journal of Veterinary Clinical pathology, 6(3): 1647-1656. [In Persian]
Baker, R.G., Hayden, M.S. and Ghosh, S. (2011). NF-κB, inflammation, and metabolic disease. Cell Metabolism, 13(1): 11-22.
Bernabucci, U., Ronchi, B., Lacetera, N. and Nardone, A. (2005). Influence of body condition score on relationships between metabolic status and oxidative stress in periparturient dairy cows. Journal of Dairy Science, 88(6): 2017-2026.
Chapinal, N., Carson, M., Duffield, T.F., Capel, M., Godden, S., Overton, M., et al. (2011). The association of serum metabolites with clinical disease during the transition period. Journal of Dairy Science, 94(10): 897-903.
Contreras, G.A., Raphael, W., Mattmiller, S.A., Gandy, J. and Sordilo, L.M. (2012). Nonesterified fatty acids modify inflammatory response and eicosanoid biosynthesis in bovine endothelial cells. Journal of Dairy Science, 95(9): 5011-5023.
Czaja, M.J. (2007). Cell signaling in oxidative stress-induced liver injury. In Seminars in Liver Disease, 27(4) : 378-389.
Dann, H.M. and Drackley, J.K. (2005). Carnitine palmitoyltransferaseI in liver of periparturient dairy cows: effects of prepartum intake, postpartum induction of ketosis, and periparturient disorders. Journal of Dairy Science, 88(11): 3851-3859.
Denk, A., Wirth, T. and Baumann, B. (2000). NF-κB transcription factors: critical regulators of hematopoiesis and neuronal survival. Cytokine and Growth Factor Reviews, 11(4): 303-320.
Dorrington, M.G. and Fraser, I.D. (2019). NF-κB signaling in macrophages: dynamics, crosstalk, and signal integration. Frontiers in Immunology, 10(1): 705-708.
Drackley, J. K. (1999). Biology of dairy cows during the transition period: The final frontier?. Journal of Dairy Science, 82(11): 2259-2273.
Du, X., Chen, L., Huang, D., Peng, Z., Zhao, C., Zhang, Y., et al. (2017). Elevated apoptosis in the liver of dairy cows with ketosis. Cellular Physiology and Biochemistry, 43(2): 568-578.
El-Bahr, S.M. and El-Deeb, W.M. (2017). Oxidative stress and cardiac biomarkers in lambs affected with enzootic ataxia: the diagnostic and prognostic significance. Veterinarski Arhiv, 87(3): 259-271.
El-Deeb, W.M. and Younis, E.E. (2009). Clinical and biochemical studies on Theileria annulata in Egyptian buffaloes (Bubalus bubalis) with particular orientation to oxidative stress and ketosis relationship. Veterinary Parasitology, 164(2-4): 301-305.
Feldstein, A.E., Werneburg, N.W., Canbay, A., Guicciardi, M.E., Bronk, S.F., Rydzewski, R., et al. (2004). Free fatty acids promote hepatic lipotoxicity by stimulating TNFα expression via a lysosomal pathway. Hepatology, 40(1): 185-194.
Fiore, F., Spissu, N., Sechi, S. and Coccom, R. (2019). Evaluation of oxidative stress in dairy cows with left displacement of abomasum. Animal, 9(11): 966.
Galvão, K.N., Flaminio, M.J.B.F., Brittin, S.B., Sper, R., Fraga, M., Caixeta, L., et al. (2010). Association between uterine disease and indicators of neutrophil and systemic energy status in lactating Holstein cows. Journal of Dairy Science, 93(7): 2926-2937.
González, F.D., Muiño, R., Pereira, V., Campos, R. and Benedito, J.L. (2011). Relationship among blood indicators of lipomobilization and hepatic function during early lactation in high-yielding dairy cows. Journal of Veterinary Science, 12(3): 251-255.
Grummer, R.R. (1995). Impact of changes in organic nutrient metabolism on feeding the transition dairy cow. Journal of Animal Science, 73(9): 2820-2833.
Hammon, D., Evjen, I.M., Dhiman, T.R., Goff, J.P. and Walters, L.J. (2006). Neutrophil function and energy status in Holstein cows with uterine health disorders. Veterinary Immunology and Immunopathology, 113(1-2): 21-29.
Hayden, M.S. and Ghosh, S. (2008). Shared principles in NF-κB signaling. Cell, 132(3): 344-362.
Hayden, M.S., West, A.P. and Ghosh, S. (2006). NF-κB and the immune response. Oncogene, 25(51): 6758-6780.
Herdt, T.H. (2000). Ruminant adaptation to negative energy balance: Influences on the etiology of ketosis and fatty liver. Veterinary Clinics of North America: Food Animal Practice, 16(2): 215-230.
Hybertson, B.M., Gao, B., Bose, S.K. and McCord, J.M. (2011). Oxidative stress in health and disease: the therapeutic potential of Nrf2 activation. Molecular Aspects of Medicine, 32(4-6): 234-246.
Ji, L.L., Gomez-Cabera, M.C. and Vina, J. (2006). Exercise and hormesis. Annals of the New York Academy of Sciences, 1067(1): 425-435.
LeBlanc, S.J., Osawa, T. and Dubuc, J. (2011). Reproductive tract defense and disease in postpartum dairy cows. Theriogenology, 76(9): 1610-1618.
Leopold, J. A. and Loscalzo, J. (2009). Oxidative risk for atherothrombotic cardiovascular disease. Free Radical Biology and Medicine, 47(12): 1673-1706.
Li, X., Chen, H., Lei, L., Liu, J., Guan, Y., Liu, Z., et al. (2013). Non-esterified fatty acids activate the AMP-activated protein kinase signaling pathway to regulate lipid metabolism in bovine hepatocytes. Cell Biochemistry and Biophysics, 67(3): 1157-1169.
Li, Y., Ding, H.Y., Wang, X.C., Feng, S.B., Li, X.B., Wang, Z., et al. (2016). An association between the level of oxidative stress and the concentrations of NEFA and BHBA in the plasma of ketotic dairy cows. Journal of Animal Physiology and Animal Nutrition, 100(5): 844-851.
Liguori, I., Russo, G., Curcio, F., Bulli, G., Aran, L., Della-Morte, D., et al. (2018). Oxidative stress, aging, and diseases. Clinical Interventions in Aging, 13: 757-772.
Liu, T., Zhang, L., Joo, D. and Sun, S.C. (2017). NF-κB signaling in inflammation. Signal Transduction and Targeted Therapy, 2: 17023.
Mezzetti, M., Cattaneo, L., Maria Passamonti, M., Lopreiato, V., Minuti, A. and Trevisi, E. (2021). The Transition Period Updated: A Review of the New Insights into the Adaptation of Dairy Cows to the New Lactation. Dairy, 2(4): 617-636.
Morgan, M.J. and Liu, Z.G. (2011). Crosstalk of reactive oxygen species and NF-κB signaling. Cell Research, 21(1): 103-115.
Nelson, D.L., Cox, M.M. and Lehninger, A.L. (2005). Principles of biochemistry. 4th ed., USA: New York, Freeman, pp: 600-622.
Niu, Q., Cao, M., Chen, S., Zhao,Y., Li, C. and Zhou, X. (2019). Effective and economical column- based method for RNA isolation from animal cells. Biotechnology Letters, 41(8-9): 915-920.
Ospina, P.A., Nydam, D.V., Stokol, T. and Overton, T.R. (2010). Evaluation of nonesterified fatty acids and beta-hydroxybutyrate in transition dairy cattle in the northeastern United States: critical thresholds for prediction of clinical diseases. Journal of Dairy Science, 93(4): 546-554.
Pedernera, M., Celi, P., García, S.C., Salvin, E.H., Barchia, I. and Fulkerson, W.J. (2010). Effect of diet, energy balance and milk production on oxidative stress in early-lactating dairy cows grazing pasture. The Veterinary Journal, 186(3): 352-357.
Pfaffl, M.W. (2001). A new mathematical model for relative quantification in real-time RT–PCR. Nucleic acids Research, 29(9): 45-49.
Puchalska, P. and Crawford, P.A. (2017). Multi-dimensional roles of ketone bodies in fuel metabolism, signaling, and therapeutics. Cell Metabolism, 25(2): 262-284.
Puppel, K., Gołębiewski, M., Solarczyk, P., Grodkowski, G., Slósarz, J., Kunowska-Slósarz, M., et al. (2019). The relationship between plasma β-hydroxybutyric acid and conjugated linoleic acid in milk as a biomarker for early diagnosis of ketosis in postpartum Polish Holstein-Friesian cows. BMC Veterinary Research, 15(1): 367.
Romagnoli, M., Gomez-Cabrera, M.C., Perrelli, M.G., Biasi, F., Pallardo, V.F., Sastre, J., et al. (2010). Xanthine oxidase-induced oxidative stress causes activation of NF-κB and inflammation in the liver of type I diabetic rats. Free Radical Biology and Medicine, 49(2): 171-177.
Sadeghi-nasab, A., Hassanpour, A., Sabaghsaray, H. and Amiri-Sadeghan, S. (2011). Assessment of thyroid hormones, insulin and magnesium in dairy cattle with subclinical ketosis. Journal of Veterinary Clinical Pathology, 5(2): 1149-1159. [In Persian]
Senoh, T., Oikawa, S., Nakada, K., Tagami, T. and Iwasaki, T. (2019). Increased serum malondialdehyde concentration in cows with subclinical ketosis. Journal of Veterinary Medical Science. 81: 817-820.
Shi, X., Li, D., Deng, Q., Li, Y., Sun, G., Yuan, X., et al. (2015). NEFAs activate the oxidative stress-mediated NF-κB signaling pathway to induce inflammatory response in calf hepatocytes. The Journal of Steroid Biochemistry and Molecular Biology, 145(8): 103-112.
Sordillo, L.M. and Aitken, S.L. (2009). Impact of oxidative stress on the health and immune function of dairy cattle. Veterinary Immunology and Immunopathology, 128(1-3): 104-109.
Sordillo, L.M. and Raphael, W. (2013). Significance of metabolic stress, lipid mobilization,and inflammation on transition cow disorders. Veterinary Clinics of North America: Food Animal Practice, 29(2): 267-278.
Stokol, T. and Nydam, D.V. (2006). Effect of hemolysis on nonesterified fatty acid and beta-hydroxybutyrate concentrations in bovine blood. Journal of Veterinary Diagnostic Investigation, 18(5): 466-469.
Suriyasathaporn, W.S., Heuer, C.H., Noordhuizen-Stassen, E.N. and Schukken, Y.H.S. (2000). Hyperketonemia and the impairment of udder defense: a review. Veterinary Research, 31(4): 397-412.
Turk, R., Juretić, D., Gereš, D., Svetina, A., Turk, N. and Flegar-Mestric, Z. (2008). Influence of oxidative stress and metabolic adaptation on PON1 activity and MDA level in transition dairy cows. Animal Reproduction Science, 108(1-2): 98-106.
Turk, R., Juretić, D., Gereš, D., Turk, N., Simeon-Rudolf, V., Rekić, B., et al. (2005). Oxidative stress in dairy cows–serum paraoxonase activity related to hepatomegaly. Croatica Chemica Acta, 78(3): 375-378.
Wang, D., Yu, D., Zhao, C., Xia, C., Xu, C. and Wu, L. (2021). Subclinical ketosis risk prediction in dairy cows based on prepartum metabolic indices. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 73(1): 11-17.
Wankhade, P.R., Manimaran, A., Kumaresan, A., Jeyakumar, S., Ramesha, K.P., Sejian, V., et al. (2017). Metabolic and immunological changes in transition dairy cows: A review. Veterinary World, 10(11): 1367-1377.
Weisberg, S.P., Hunter, D., Huber, R., Lemieux, J., Slaymaker, S., Vaddi, K., et al. (2006). CCR2 modulates inflammatory and metabolic effects of high-fat feeding. Journal of Clinical Investigation, 116(1): 115-124.
Wree, A., Schlattjan, M., Bechmann, L.P., Claudel, T., Sowa, J.P., Stojakovic, T., et al. (2014). Adipocyte cell size, free fatty acids and apolipoproteins are associated with non-alcoholic liver injury progression in severely obese patients. Metabolism: Clinical and Experimental, 63(12): 1542-1552.
