اثر سانتریفیوژ و کلسترول بارگذاری‌شده با سیکلودکسترین در رقیق‌کننده لیسیتین سویا بر‌کیفیت اسپرم قوچ قزل بعد از فرآیند ذوب
الموضوعات :
محمد شمس الهی
1
(دانشجوی دکترای تخصصی گروه علوم دامی، دانشکده کشاورزی، دانشگاه تبریز، تبریز، ایران.)
حسین دقیق کیا
2
(استاد گروه علوم دامی، دانشکده کشاورزی، دانشگاه تبریز، تبریز، ایران.)
تاريخ الإرسال : 17 الجمعة , رمضان, 1439
تاريخ التأكيد : 26 الأربعاء , شعبان, 1440
تاريخ الإصدار : 25 الجمعة , ربيع الأول, 1441
الکلمات المفتاحية:
اسپرم,
قوچ,
سانتریفیوژ,
سیکلودکسترین,
انجماد-ذوب,
ملخص المقالة :
حفظ تحرک و زنده مانی اسپرم قوچ هنگام استفاده از اسپرم منجمد شده برای به دست آوردن میزان آبستنی بالا مهم است. هدف از این مطالعه بررسی اثر سانتریفیوژ و کلسترول بارگذاری شده با سیکلودکسترین در لیسیتین سویا به عنوان رقیق کننده، بر کیفیت اسپرم بعد از فرایند انجماد-ذوب بود. نمونه‌های منی از 5 رأس قوچ قزل 5-4 ساله به مدت 3 هفته جمع‌آوری شدند. بعد از ارزیابی اولیه و تائید کیفیت، نمونه‌ها به 8 قسمت مساوی تقسیم شدند. پلاسمای منی 4 نمونه در دمای 30 درجه سلسیوس به وسیله سانتریفیوژ حذف گردید. نمونه‌های باقی مانده بدون سانتریفیوژ به وسیله بافر تریس و غلظت‌های مختلف کلسترول لودشده با سیکلودکسترین (0، 75/0، 5/1 و 3 میلی‌گرم در 108×2/1 اسپرم) و 7 درصد گلیسرول مشابه با گروه اول رقیق‌سازی شدند. سپس نمونه‌ها تا دمای 5 درجه سلسیوس سردسازی شده و در پایوت‌های 25/0 میلی‌لیتری ذخیره شدند. نتایج نشان‌دهنده بهبود معنی‌دار پارامترهای تحرک کل و پیش‌رونده، سرعت در مسیر مستقیم، سرعت در مسیر منحنی، تناوب عرضی زنش، یکپارچگی غشای آکروزومی، یکپارچگی غشای‌پلاسمایی، ظرفیت تام آنتی اکسیدانی و فعالیت آنزیم های سوپراکسید دیسموتاز، گلوتاتیون پراکسیداز و مالون دی‌آلدئید، بعد از ذوب در گروه دریافت کننده 5/1 میلی‌گرم سیکلودکسترین در مقایسه با سایر گروه ها بود (05/0p <). همچنین تحرک کل و پیش‌رونده، سرعت در مسیر منحنی، یکپارچگی غشای پلاسمایی، ظرفیت تام آنتی‌اکسیدانی و فعالیت آنزیم های سوپراکسید دیسموتاز و گلوتاتیون پراکسیداز به طور معنی‌داری در گروه بدون سانتریفیوژ نسبت به گروه با سانتریفیوژ، بالاتر بود (05/0p <). به طورکلی نتایج نشان داد که افزودن 5/1 میلی‌گرم کلسترول بارگذاری شده با سیکلودکسترین باعث بهبود اغلب پارامترهای مورد ارزیابی نسبت به سایر گروه‌ها شد.
المصادر:
Ahmad, E., Aksoy, M., Serin, I., Kucuk, N., Ceylan, A., Ucan, U., et al. (2013). Cholesterol-loaded cyclodextrin pretreatment of ram spermatozoa protects structural integrity of plasma membrane during osmotic challenge and reduces their ability to undergo acrosome reaction in vitro. Small Ruminant Research, 115(1-3): 77-81.
Awad, M.M. (2011). Effects of sub-optimal glycerol concentration and cholesterol-loaded cyclodextrin in a Tris-based diluent on cryopreserved ram sperm longevity and acrosomal integrity. Small Ruminant Research, 100(2-3): 164-168.
Bilodeau, J.F., Chatterjee, S., Sirard, M.A. and Gagnon, C. (2000). Levels of antioxidant defenses are decreased in bovine spermatozoa after a cycle of freezing and thawing. Molecullar Reproduction and Development, 55(3): 282-288.
Bucak, M.N., Atessahin, A., Varisli, Ö., Yüce, A., Tekin, N., Akcay, A., et al. (2007). Influence of trehalose, taurine, cysteamine and hyaluronan on ram semen microscopic and oxidative stress parameters after freeze thawing process. Theriogenology, 67(5): 1060-1067.
Combes, G.B., Varner, F., Schroeder, R.C. and Burghardt, T.L. (2000). Effect of cholesterol on the motility and plasma membrane integrity of frozen equine spermatozoa after thawing. Journal of reproduction and fertility. Supplement, 56(56): 127-132.
Cross, N.L. (1998). Role of cholesterol in sperm capacitation, Biology Reproduction, 59(1): 7-11.
Cross, N.L. (2003). Decrease in order of human sperm lipids during capacitation. Biology of Reproduction, 69(2): 529-534.
Darin-Bennett, A. and White, I.G. (1977). Influence of the cholesterol content of mammalian spermatozoa on susceptibility to cold-shock. Cryobiology, 14(4): 466-470.
Del, E., Olmo, A., Bisbal, A., Maroto-Morales, O., Garcia-Alvarez, M., Ramon, P., et al. (2013). Fertility of cryopreserved ovine semen is determined by sperm velocity. Animal Reproduction Science, 138(1-2): 102-109.
Elizabeth, G., Crichton, A., Budhan, S., Pukazhenthi, B., Billah, A., Julian, A., et al. (2015). Cholesterol addition aids the cryopreservation of dromedary camel (Camelus dromedarius) spermatozoa. Theriogenology, 83(2): 168-174.
Ellington, J.E., Samper, J.C., Jones, A.E., Oliver, S.A., Burnett, K.M., Wright, R.W., et al. (1999). In vitro interactions of cryopreserved stallion spermatozoa and oviduct (uterine tube) epithelial cells or their secretory products. Animal Reproduction Science, 56(1): 51-65.
Esterbauer, H. and Cheeseman, K.H. (1990). Determination of aldehydic lipid peroxidation products: malonaldehyde and 4-hydroxynonenal. Methods in Enzymology, 186 407-421.
Futino, D., Mendes, M., Matos, W., Mondadori, R. and Lucci, C. (2010). Glycerol, methyl-formamide and dimethyl-formamide in canine semen cryopreservation. Reproduction in Domestic Animals, 45(2): 214-220.
Gil, J., Söderquist, L. and Rodríguez-Martínez, H. (1999). Influence of centrifugation and different extenders on post-thaw sperm quality of ram sperm. Theriogenology, 54(1): 93-108.
Hancock, J.L. (1956). The morphology of boar spermatozoa. Journal of the Royal Microscopical Society, 76(3): 84-97.
Holt, W.V. (2000). Fundamental aspects of sperm cryobiology: the importance of species and individual differences. Theriogenology, 53(1): 47-58.
Miller, J.K., Brzezinska-Slebodzinska, E. and Madsen, F.C. (1993). Oxidative stress, antioxidants, and animal function. Journal of Dairy Science, 76(9): 2812-2823.
Moore, A.I., Squires, E.L. and Graham, J.K. (2005). Adding cholesterol to the stallion sperm plasma membrane improves cryosurvival. Cryobiology, 51(3): 241-249.
Naseer, Z., Ahmad, E., Aksoy, M., Küçük, N., Serin, İ., Ceylan, A., et al. (2015). Protective effect of cholesterol-loaded cyclodextrin pretreatment against hydrogen peroxide induced oxidative damage in ram sperm. Cryobiology, 71(1): 18-23.
Ochsendorf, F. (1999). Infections in the male genital tract and reactive oxygen species. Human Reproduction Update, 5(5): 399-420.
Ohvo-Rekila, H., Ramstedt, B., Leppimaki, P. and Slotte J.P. (2002). Cholesterol interactions with phospholipids in membranes. Progress Lipid Research, 41(1): 66-97.
Partyka, A., Łukaszewicz, E. and Niżański, W. (2012a). Effect of cryopreservation on sperm parameters, lipid peroxidation and antioxidant enzymes activity in fowl semen. Theriogenology, 77(8): 1497-1504.
Partyka, A., Łukaszewicz, E. and Niżański, W. (2012b). Lipid peroxidation and antioxidant enzymes activity in avian semen. Animal Reproduction Science, 134(3-4): 184-190.
Purdy, P.H. and Graham J.K. (2004). Effect of cholesterol-loaded cyclodextrin on the cryosurvival of bull sperm. Cryobiology, 48(1): 36-45.
Ritar, A.J. (1993). Control of ovulation, storage of semen and artificial insemination of fiber-producing goats in Australia a review. Australian Journal of Experimental Agriculture, 33: 807-820.
Roostaei, A.M.M., Mousavi, M. and Ghadamyari, M. (2015). Effect of seminal plasma proteins on membrane cholesterol efflux of ram epididymal spermatozoa. Small Ruminant Research, 129: 88-91.
Salmani, H., Nabi, M.M., Vaseghi-Dodaran, H., Rahman, M.B., Mohammadi-Sangcheshmeh, A., Shakeri, M., et al. (2013). Effect of glutathione in soybean lecithin-based semen extender on goat semen quality after freeze-thawing. Small Ruminant Research, 112(1-3): 123-127.
Serin, I., Aksoy, M. and Ceylan, A. (2011). Cholesterol-loaded cyclodextrin inhibits premature acrosomal reactions in liquid-stored rabbit spermatozoa. Animal Reproduction Science, 123(1-2): 106-111.
Sion, B., Janny, L., Boucher, D. and Grizard, G. (2004). Annexin V binding to plasma membrane predicts the quality of human cryopreserved spermatozoa. International Journal of Andrology, 27(2): 108-114.
Thys, M., Nauwynck, H., Maes, D., Hoogewijs, M., Vercauteren, D., Rijsselaere, T., Favoreel, H., Van Soom, A., et al. (2009). Expression and putative function of fibronectin and its receptor (integrin a5b1) in male and female gametes during bovine fertilization in vitro. Reproduction 138(3): 471-482.
Upreti, G.C., Hall, E.L., Koppens, D., Oliver, J.E. and Vishwanath, R. (1999). Studies on the measurement of phospholipase A2 (PLA2) and PLA2 inhibitor activities in ram semen. Animal Reproduction Science, 56(2): 107-121.
Witte, T.S. and Schäfer-Somi, S. (2007). Involvement of cholesterol, calcium and progesterone in the induction of capacitation and acrosome reaction of mammalian spermatozoa. Animal and Reproduction Science, 102(3-4): 181-193.
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Ahmad, E., Aksoy, M., Serin, I., Kucuk, N., Ceylan, A., Ucan, U., et al. (2013). Cholesterol-loaded cyclodextrin pretreatment of ram spermatozoa protects structural integrity of plasma membrane during osmotic challenge and reduces their ability to undergo acrosome reaction in vitro. Small Ruminant Research, 115(1-3): 77-81.
Awad, M.M. (2011). Effects of sub-optimal glycerol concentration and cholesterol-loaded cyclodextrin in a Tris-based diluent on cryopreserved ram sperm longevity and acrosomal integrity. Small Ruminant Research, 100(2-3): 164-168.
Bilodeau, J.F., Chatterjee, S., Sirard, M.A. and Gagnon, C. (2000). Levels of antioxidant defenses are decreased in bovine spermatozoa after a cycle of freezing and thawing. Molecullar Reproduction and Development, 55(3): 282-288.
Bucak, M.N., Atessahin, A., Varisli, Ö., Yüce, A., Tekin, N., Akcay, A., et al. (2007). Influence of trehalose, taurine, cysteamine and hyaluronan on ram semen microscopic and oxidative stress parameters after freeze thawing process. Theriogenology, 67(5): 1060-1067.
Combes, G.B., Varner, F., Schroeder, R.C. and Burghardt, T.L. (2000). Effect of cholesterol on the motility and plasma membrane integrity of frozen equine spermatozoa after thawing. Journal of reproduction and fertility. Supplement, 56(56): 127-132.
Cross, N.L. (1998). Role of cholesterol in sperm capacitation, Biology Reproduction, 59(1): 7-11.
Cross, N.L. (2003). Decrease in order of human sperm lipids during capacitation. Biology of Reproduction, 69(2): 529-534.
Darin-Bennett, A. and White, I.G. (1977). Influence of the cholesterol content of mammalian spermatozoa on susceptibility to cold-shock. Cryobiology, 14(4): 466-470.
Del, E., Olmo, A., Bisbal, A., Maroto-Morales, O., Garcia-Alvarez, M., Ramon, P., et al. (2013). Fertility of cryopreserved ovine semen is determined by sperm velocity. Animal Reproduction Science, 138(1-2): 102-109.
Elizabeth, G., Crichton, A., Budhan, S., Pukazhenthi, B., Billah, A., Julian, A., et al. (2015). Cholesterol addition aids the cryopreservation of dromedary camel (Camelus dromedarius) spermatozoa. Theriogenology, 83(2): 168-174.
Ellington, J.E., Samper, J.C., Jones, A.E., Oliver, S.A., Burnett, K.M., Wright, R.W., et al. (1999). In vitro interactions of cryopreserved stallion spermatozoa and oviduct (uterine tube) epithelial cells or their secretory products. Animal Reproduction Science, 56(1): 51-65.
Esterbauer, H. and Cheeseman, K.H. (1990). Determination of aldehydic lipid peroxidation products: malonaldehyde and 4-hydroxynonenal. Methods in Enzymology, 186 407-421.
Futino, D., Mendes, M., Matos, W., Mondadori, R. and Lucci, C. (2010). Glycerol, methyl-formamide and dimethyl-formamide in canine semen cryopreservation. Reproduction in Domestic Animals, 45(2): 214-220.
Gil, J., Söderquist, L. and Rodríguez-Martínez, H. (1999). Influence of centrifugation and different extenders on post-thaw sperm quality of ram sperm. Theriogenology, 54(1): 93-108.
Hancock, J.L. (1956). The morphology of boar spermatozoa. Journal of the Royal Microscopical Society, 76(3): 84-97.
Holt, W.V. (2000). Fundamental aspects of sperm cryobiology: the importance of species and individual differences. Theriogenology, 53(1): 47-58.
Miller, J.K., Brzezinska-Slebodzinska, E. and Madsen, F.C. (1993). Oxidative stress, antioxidants, and animal function. Journal of Dairy Science, 76(9): 2812-2823.
Moore, A.I., Squires, E.L. and Graham, J.K. (2005). Adding cholesterol to the stallion sperm plasma membrane improves cryosurvival. Cryobiology, 51(3): 241-249.
Naseer, Z., Ahmad, E., Aksoy, M., Küçük, N., Serin, İ., Ceylan, A., et al. (2015). Protective effect of cholesterol-loaded cyclodextrin pretreatment against hydrogen peroxide induced oxidative damage in ram sperm. Cryobiology, 71(1): 18-23.
Ochsendorf, F. (1999). Infections in the male genital tract and reactive oxygen species. Human Reproduction Update, 5(5): 399-420.
Ohvo-Rekila, H., Ramstedt, B., Leppimaki, P. and Slotte J.P. (2002). Cholesterol interactions with phospholipids in membranes. Progress Lipid Research, 41(1): 66-97.
Partyka, A., Łukaszewicz, E. and Niżański, W. (2012a). Effect of cryopreservation on sperm parameters, lipid peroxidation and antioxidant enzymes activity in fowl semen. Theriogenology, 77(8): 1497-1504.
Partyka, A., Łukaszewicz, E. and Niżański, W. (2012b). Lipid peroxidation and antioxidant enzymes activity in avian semen. Animal Reproduction Science, 134(3-4): 184-190.
Purdy, P.H. and Graham J.K. (2004). Effect of cholesterol-loaded cyclodextrin on the cryosurvival of bull sperm. Cryobiology, 48(1): 36-45.
Ritar, A.J. (1993). Control of ovulation, storage of semen and artificial insemination of fiber-producing goats in Australia a review. Australian Journal of Experimental Agriculture, 33: 807-820.
Roostaei, A.M.M., Mousavi, M. and Ghadamyari, M. (2015). Effect of seminal plasma proteins on membrane cholesterol efflux of ram epididymal spermatozoa. Small Ruminant Research, 129: 88-91.
Salmani, H., Nabi, M.M., Vaseghi-Dodaran, H., Rahman, M.B., Mohammadi-Sangcheshmeh, A., Shakeri, M., et al. (2013). Effect of glutathione in soybean lecithin-based semen extender on goat semen quality after freeze-thawing. Small Ruminant Research, 112(1-3): 123-127.
Serin, I., Aksoy, M. and Ceylan, A. (2011). Cholesterol-loaded cyclodextrin inhibits premature acrosomal reactions in liquid-stored rabbit spermatozoa. Animal Reproduction Science, 123(1-2): 106-111.
Sion, B., Janny, L., Boucher, D. and Grizard, G. (2004). Annexin V binding to plasma membrane predicts the quality of human cryopreserved spermatozoa. International Journal of Andrology, 27(2): 108-114.
Thys, M., Nauwynck, H., Maes, D., Hoogewijs, M., Vercauteren, D., Rijsselaere, T., Favoreel, H., Van Soom, A., et al. (2009). Expression and putative function of fibronectin and its receptor (integrin a5b1) in male and female gametes during bovine fertilization in vitro. Reproduction 138(3): 471-482.
Upreti, G.C., Hall, E.L., Koppens, D., Oliver, J.E. and Vishwanath, R. (1999). Studies on the measurement of phospholipase A2 (PLA2) and PLA2 inhibitor activities in ram semen. Animal Reproduction Science, 56(2): 107-121.
Witte, T.S. and Schäfer-Somi, S. (2007). Involvement of cholesterol, calcium and progesterone in the induction of capacitation and acrosome reaction of mammalian spermatozoa. Animal and Reproduction Science, 102(3-4): 181-193.
