اثرات نانوکامپوزیت گرافن اکسید کوت شده با ۸-هیدروکسی کینولین بر روی مرگ سلولی و آپوپتوز در رده های سلولی سرطان پستان(MCF-7) و سرطان کولون(SW742)
الموضوعات : مجله پلاسما و نشانگرهای زیستیفیروزه خیل تاش 1 , کاظم پریور 2 , نسیم حیاتی رودباری 3 , علیرضا بدیعی 4 , بهنام صادقی 5
1 - گروه زیست شناسی، دانشکده علوم، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران
2 - گروه زیست شناسی، دانشکده علوم، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران
3 - گروه زیست شناسی، دانشکده علوم، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران
4 - گروه شیمی، دانشکده علوم، دانشگاه تهران، تهران، ایران.
5 - گروه ایمنی درمانی سرطان و پزشکی بازساختی، مرکز تحقیقات سرطان پستان، پژوهشکده معتمد، جهاد دانشگاهی، تهران، ایران.
الکلمات المفتاحية: سرطان پستان, آپوپتوز, گرافن اکسید, سرطان کولون, ۸-هیدروکسی کینولین,
ملخص المقالة :
زمینه و هدف: سرطان پستان در زنان و سرطان کولورکتال در هر دو جنس جزو شایع ترین و کشنده ترین سرطان ها در سراسر جهان می باشند.با توجه به مقاوم بودن برخی از سرطان ها به درمان های رایج، در این تحقیق اثرات سمیت و القائ آپوپتوز نانوکامپوزیت گرافن اکساید کوت شده با ۸-هیدروکسی کینولین بر روی سلول های رده سرطان پستان و کولون سنجیده شد. روش کار: در این مطالعهرده سلولی سرطان پستان(MCF-7)، سرطان کولون(SW-742) و رده سلول نرمال پستان(MCF-10) را با دوزهای مختلف نانوصفحات گرافن اکساید کوت شده با ۸-هیدروکسی کینولینبه مدت ۱۲، ۲۴ و ۴۸ ساعت تیمار و سمیت این نانوکامپوزیت را با روش MTT و تاثیر ماده فوق الذکر در آپوپتوز سلولی هم از طریق بررسی بیان ژن های پیش برنده و مهار کننده آپوپتوز از جمله P53, P21, Bax و Bcl-2 نیز از طریق تست فلوسایتومتری آپوپتوز سلولی(Annexin-V/PI) صورت گرفته شد. یافته ها: نتایج نشان دادند که نانوکامپوزیت سبب افزایش معنادار مرگ سلولی در رده های سلولی سرطان کولون و پستان به خصوص رده MCF-7 در مقایسه با رده سلول نرمال پستان گردیده است. نتایج بیان ژن نیز حاکی از افزایش بیان ژن های پیش آپوپتوزی P53, p21 و Bax و کاهش بیان ژن ضد آپوپتوزی Bcl-2 در رده های سلول سرطانی به خصوص رده MCF-7 به نسبت رده سلول نرمال پستان است. نتایج فلوسایتومتری تست Annexin-V/PI نیز در همین راستا القائ آپوپتوز در رده های سرطانی بالاخص MCF-7 به نسبت نمونه کنترل را پس از تیمار با نانوکامپوزیت نشان داد. نتیجه گیری: یکی از مکانیسم های القائ مرگ سلولی استفاده از نانوکامپوزیت با القائ آپوپتوز در آن ها می باشد.
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