اثرات افزودن روغن‎های اسانسی (آویشن و پونه‎ ی کوهی) و فیتاز میکروبی بر میکروفلور روده و سطح ایمنی (ایمنی همورال (HI) و سلول‌های خونی) در جوجه ‎های گوشتی

الموضوعات : مجله پلاسما و نشانگرهای زیستی

اسلام قلندری ¹ , علیرضا صفامهر ² , علی نوبخت ³ , یوسف مهمان نواز ⁴ , سامان مهدوی ⁵

1 - دانشجوی تغذیه دام گروه علوم دامی دانشگاه آزاد اسلامی واحد مراغه، مراغه، ایران
2 - استاد گروه علوم دامی دانشگاه آزاد اسلامی واحد مراغه، مراغه، ایران.
3 - دانشیار گروه علوم دامی دانشگاه آزاد اسلامی واحد مراغه، مراغه، ایران.
4 - استادیارگروه علوم دامی دانشگاه آزاد اسلامی واحد مراغه، مراغه، ایران.
5 - استادیار گروه میکروبیولوژی دانشگاه آزاد اسلامی واحد مراغه، مراغه، ایران.

تاريخ الإرسال : 04 الأربعاء , صفر, 1444 تاريخ التأكيد : 21 الإثنين , ربيع الأول, 1444 تاريخ الإصدار : 28 الخميس , جمادى الأولى, 1444

الکلمات المفتاحية: میکروفلور روده, اسانس, فیتاز میکروبی, ایمنی, جوجه گوشتی,

ملخص المقالة :

زمینه و هدف: با اینکه در تغذیه‌ی طیور از جمله جوجه‌های گوشتی از گیاهان دارویی به طرق مختلف استفاده شده است، اما استفاده‌ی همزمان از اسانس‌ها و آنزیم‌ها، بخصوص آنزیم فیتاز میکروبی کمتر استفاده شده است. آویشن و پونه گیاهی هستند که بر سیستم ایمنی موثر می‌باشند. اثرات مثبت فیتاز در طیور مستند شده است. هدف از تحقیق حاضر، بررسی اثرات متقابل روغن‌های اسانسی و آنزیم فیتاز بر میکروفلور روده و سطح ایمنی جوجه‌های گوشتی می‌باشد.مواد و روش ها: این آزمایش به بررسی میکروفلور روده و سطح ایمنی (ایمنی همورال (تست HI) و سلول‌های خونی) با استفاده از سه سطح اسانس آویشن، اسانس پونه و فیتاز میکروبی با 8 تیمار و 4 تکرار و مجموعاً با 32 واحد آزمایشی به مدت 42 روز، روی جوجه‌های گوشتی انجام گرفت. برای بررسی میکروفلور روده و سطح ایمنی به ترتیب از روش‌های کشت ( MRS-Agar و EMB) و روش الایزا و استفاده از کیت‌های تجاری زیست شیمی استفاده شد.نتایج: بررسی اثرات اصلی، نشان داد تیمارهایی که اسانس آویشن مصرف کرده بودند، در مقایسه با سایر تیمار ها جمعیت کلی‌فرم و کل باکتری‎های هوازی TAB)) کمتری داشتند (05/0p<). سطح ایمنی همورال تفاوت معنی‌داری در بین گروه‌های مختلف آزمایشی نشان داد (05/0p<). تیمارهایی که اسانس‌های آویشن و پونه را دریافت نکرده بودند، کمترین نسبت هتروفیل به لنفوسیت ها را داشتند (05/0p<).نتیجه‌گیری: نتایج نشان داد استفاده از آنزیم فیتاز میکروبی و اسانس آویشن، جمعیت باکتری‌های کلی‌فرم روده را کاهش می‌دهد و استفاده از اسانس آویشن، باعث بهبود ایمنی همورال می‌گردد.

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