بررسی تاثیر باکتری اشرشیا کُلی (Escherichia coli) بر بافت بیضه در موش صحرایی
الموضوعات : پاتوبیولوژی مقایسه ای
مینا فلاح، آرش خاکی، بهبود جعفری .
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الکلمات المفتاحية: باکتری اشرشیا کلی, ناباروری, بافت بیضه, پارامترهای اسپرم,
ملخص المقالة :
بیماریهای عفونی دستگاه تناسلی شامل میکروب‌ها و ویروس‌هایی است که از طریق ارتباط جنسی منتقل می‌شوند. سفلیس و سوزاک عفونتهای قدیمی تناسلی هستند، اما امروزه بیماریهای دیگری مانند ایدز و آنتی‌ژن‌هایی که از طریق رابطه جنسی منتقل می‌شوند نیز مطرح هستند. هدف از مطالعه حاضر بررسی تاثیر باکتری‌اشرشیا کلی بر بافت بیضه و لوله های اسپرم ساز و پارامترهای مربوط به اسپرم بود. تعداد 16 سر موش صحرایی نر بالغ نژاد ویستار با وزن متوسط 200 تا 220 گرم با سن 5/2 تا 3 ماه به دو گروه 8 تایی تقسیم شده و در طی 60 روز دوره مطالعه، حیوانات در معرض 12ساعت تاریکی و 12ساعت روشنایی قرار گرفتند. سوش اشرشیاکلی انتروتوکسیژنیک (سروتایپ-0114) از آزمایشگاه مرجع بوعلی تهران تهیه و جهت عفونی نمودن موش‌ها استفاده شد. پس از ایجاد عفونت از بافت اسپرم نمونه آسیب شناسی تهیه و با رنگ آمیزی هماتوکسیلین ـ ائوزین بررسی شد. همچنینن میزان تحرک، قابلیت زیست و تعداد اسپرم‌ها نیز ارزیابی گردید. نتایج مطالعه بافتی نشان داد در گروه شاهد تمامی لوله های اسپرم ساز به همدیگر چسبیده و تمامی رده های سلولهای زاینده جنسی قابل مشاهده بود. درحالیکه در گروه دریافت کننده اشرشیا کلی، لوله های اسپرم ساز از هم گسیخته شده و رده های سلولی جنسی از بین رفته اند. نتایج بررسی پارامترهای اسپرم نشان داد، که میزان تعداد اسپرم، قابلیت زیست اسپرم و تحرک اسپرم بصورت معنی داری در گروه آلوده به باکتری ای کلی نسبت به گروه شاهد کاهش می یابد. نتایج مطالعه نشان داد که باکتری اشرشیا کلی بصورت مشخصی بر میزان باروری و بافت بیضه تاثیر نامطلوب داشته و می‌تواند باعث کاهش باروری گردد.
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