Effect of Whey protein supplementation on GH-1 and IGF-1 in taekwondo athletes
الموضوعات : Journal of Physical Activity and Hormones
Arman Ataiee
1
,
Yasaman Shafaati
2
,
Anahita Shabani
3
1 - Department of Physical Education and Sport Sciences, Ra.C., Islamic Azad University, Rasht, Iran
2 - Department of Physical Education and Sport Sciences, Ra.C., Islamic Azad University, Rasht, Iran
3 - General Practitioner, Medicafit Corrective Exercise & Sport Medicine Clinic, Rasht, Iran
الکلمات المفتاحية: Whey Supplements, Physical Fitness, Taekwondo, Athlete.GH, IGF-1,
ملخص المقالة :
Introduction: Improving athletic performance has been one of the issues that athletes have long faced. Hundreds of sports supplements for athletes have been introduced to the market in recent years. The overall aim of the present study was to investigate the interactive effect of whey supplementation and resistance training on growth hormone and IGF-1 levels in taekwondo athletes.
Material & Methods: The method of the present study was a quasi-experimental and clinical trial. 24 taekwondo athletes were selected with an average age of 26.4±5.3 years, weight 73.34±11.6 kg, and BMI 21.81±5.4 kg/m2, and volunteers were randomly assigned into two groups: exercise + whey supplementation (group 1) and exercise (group 2). Before performing the exercise and supplementation program (50g daily in group 1), GH-1 and IGF-1, and physical fitness were measured, and after performing the exercise, testing was performed again for all variables. Data analysis using tests of Kolmogorov-Smirnov and t-test, conducted through SPSS-20 software (p≤0.05).
Results: the amount of GH and IGF1 increased in two groups compared with before training. Statistical analysis using the results indicated that after eight weeks of a combination exercise program and supplementation, strength, endurance, and agility changed significantly in both groups(p<0.05), with no significant changes in BMI and velocity(p>0.05).
Conclusion: The results showed that whey supplementation doesn’t increase GH-1, IGF-1, and physical fitness of taekwondo athletes.
1. Zhao S, Zhang H, Xu Y, Li J, Du S, Ning Z. The effect of protein intake on athletic performance: a systematic review and meta-analysis. Frontiers in Nutrition. 2024 Nov 6;11:1455728. https://doi.org/10.3389/fnut.2024.1455728
2. Witard OC, Hearris M, Morgan PT. Protein nutrition for endurance athletes: A metabolic focus on promoting recovery and training adaptation. Sports Medicine (Auckland, NZ). 2025 Mar 21;55(6):1361. doi: 10.1007/s40279-025-02203-8
3. Van Every DW, D’Souza AC, Phillips SM. Hormones, hypertrophy, and hype: an evidence-guided primer on endogenous endocrine influences on exercise-induced muscle hypertrophy. Exercise and Sport Sciences Reviews. 2024 Oct 1;52(4):117-25. DOI: 10.1249/JES.0000000000000346
4. Ye G, Xiao Z, Luo Z, Huang X, Abdelrahim ME, Huang W. Resistance training effect on serum insulin-like growth factor 1 in the serum: a meta-analysis. The Aging Male. 2020 Dec 4;23(5):1471-9. https://doi.org/10.1080/13685538.2020.1801622
5. Gharahdaghi N, Phillips BE, Szewczyk NJ, Smith K, Wilkinson DJ, Atherton PJ. Links between testosterone, oestrogen, and the growth hormone/insulin-like growth factor axis and resistance exercise muscle adaptations. Frontiers in physiology. 2021 Jan 15;11:621226. https://doi.org/10.3389/fphys.2020.621226
6. Nijenhuis-Noort EC, Berk KA, Neggers SJ, van der Lely AJ. The fascinating interplay between growth hormone, insulin-like growth factor-1, and insulin. Endocrinology and Metabolism. 2024 Jan 9;39(1):83-9. DOI: https://doi.org/10.3803/EnM.2024.101
7. Yuen KC, Hjortebjerg R, Ganeshalingam AA, Clemmons DR, Frystyk J. Growth hormone/insulin-like growth factor I axis in health and disease states: an update on the role of intra-portal insulin. Frontiers in endocrinology. 2024 Nov 21;15:1456195. https://doi.org/10.3389/fendo.2024.1456195
8. Schiaffino S, Mammucari C. Regulation of skeletal muscle growth by the IGF1-Akt/PKB pathway: insights from genetic models. Skeletal muscle. 2011 Jan 24;1(1):4.
9. Yoshida T, Delafontaine P. Mechanisms of IGF-1-mediated regulation of skeletal muscle hypertrophy and atrophy. Cells. 2020 Aug 26;9(9):1970. https://doi.org/10.3390/cells9091970
10. Mennitti C, Farina G, Imperatore A, De Fonzo G, Gentile A, La Civita E, Carbone G, De Simone RR, Di Iorio MR, Tinto N, Frisso G. How does physical activity modulate hormone responses?. Biomolecules. 2024 Nov 7;14(11):1418. https://doi.org/10.3390/biom14111418
11. Van Every DW, D’Souza AC, Phillips SM. Hormones, hypertrophy, and hype: an evidence-guided primer on endogenous endocrine influences on exercise-induced muscle hypertrophy. Exercise and Sport Sciences Reviews. 2024 Oct 1;52(4):117-25. DOI: 10.1249/JES.0000000000000346
12. Bernárdez-Vázquez R, Raya-González J, Castillo D, Beato M. Resistance training variables for optimization of muscle hypertrophy: An umbrella review. Frontiers in sports and active living. 2022 Jul 4;4:949021. https://doi.org/10.3389/fspor.2022.949021
13. Vilaça-Alves J, Magalhães PS, Rosa CV, Reis VM, Garrido ND, Payan-Carreira R, Neto GR, Costa PB. Acute hormonal responses to multi-joint resistance exercises with blood flow restriction. Journal of Functional Morphology and Kinesiology. 2022 Dec 22;8(1):3. https://doi.org/10.3390/jfmk8010003
14. Kotikangas J, Walker S, Toivonen S, Peltonen H, Häkkinen K. Acute neuromuscular and hormonal responses to power, strength, and hypertrophic protocols and training background. Frontiers in Sports and Active Living. 2022 Jul 14;4:919228. https://doi.org/10.3389/fspor.2022.919228
15. Taati B, Arazi H, Bridge CA, Franchini E. A new taekwondo- field test for estimating aerobic power, anaerobic fitness, and agility performance. Plos one. 2022 Mar 16;17(3):e0264910. https://doi.org/10.1371/journal.pone.0264910
16. Kim JW, Nam SS. Physical characteristics and physical fitness profiles of korean taekwondo athletes: A systematic review. International Journal of Environmental Research and Public Health. 2021 Sep 13;18(18):9624. https://doi.org/10.3390/ijerph18189624
17. Khazaei L, Parnow A, Amani-Shalamzari S. Comparing the effects of traditional resistance training and functional training on the bio-motor capacities of female elite taekwondo athletes. BMC Sports Science, Medicine and Rehabilitation. 2023 Oct 20;15(1):139.https://doi.org/10.1186/s13102-023-00754-9
18. Ojeda-Aravena A, Herrera-Valenzuela T, Valdés-Badilla P, Cancino-López J, Zapata-Bastias J, García-García JM. Inter-Individual variability of a high-intensity interval training with specific techniques vs. repeated sprints program in sport-related fitness of taekwondo athletes. Frontiers in Physiology. 2021 Nov 17;12:766153.https://doi.org/10.3389/fphys.2021.766153
19. Jiang G, Qin S, Yan B, Girard O. Metabolic and hormonal responses to acute high-load resistance exercise in normobaric hypoxia using a saturation clamp. Frontiers in Physiology. 2024 Sep 2;15:1445229.DOI 10.3389/fphys.2024.1445229
20. Weakley J, Schoenfeld BJ, Ljungberg J, Halson SL, Phillips SM. Physiological responses and adaptations to lower load resistance training: Implications for health and performance. Sports medicine-open. 2023 May 12;9(1):28.https://doi.org/10.1186/s40798-023-00578-4
21. Noh KW, Park S. Effects of resistance training and protein supplementation interventions on muscle volume and muscle function: sex differences in humans. Physical Activity and Nutrition. 2023 Dec 31;27(4):15.doi: 10.20463/pan.2023.0033
22. Ji X, Ye X, Ji S, Zhang S, Wang Y, Zhou Z, Xiang D, Luo B. Whey Protein Supplementation Combined with Exercise on Muscle Protein Synthesis and the AKT/mTOR Pathway in Healthy Adults: A Systematic Review and Meta-Analysis. Nutrients. 2025 Aug 8;17(16):2579.https://doi.org/10.3390/nu17162579
23. Antonio J, Evans C, Ferrando AA, Stout JR, Antonio B, Cintineo HP, Harty P, Arent SM, Candow DG, Forbes SC, Kerksick CM. Common questions and misconceptions about protein supplementation: what does the scientific evidence really show?. Journal of the International Society of Sports Nutrition. 2024 Dec 31;21(1):2341903.https://doi.org/10.1080/15502783.2024.2341903
24. Tang C, Xi T, Zheng J, Cui X. Chemical Properties of Whey Protein in Protein Powders and Its Impact on Muscle Growth in Athletes: A Review. Natural Product Communications. 2025 Mar;20(3):1934578X251326124.https://doi.org/10.1177/1934578X251326124
25. Pearson AG, Hind K, Macnaughton LS. The impact of dietary protein supplementation on recovery from resistance exercise-induced muscle damage: A systematic review with meta-analysis. European Journal of Clinical Nutrition. 2023 Aug;77(8):767-83.https://doi.org/10.1038/s41430-022-01250-y
26. Tang C, Xi T, Zheng J, Cui X. Chemical Properties of Whey Protein in Protein Powders and Its Impact on Muscle Growth in Athletes: A Review. Natural Product Communications. 2025 Mar;20(3):1934578X251326124.https://doi.org/10.1177/1934578X251326124
27. Karimi FM, Sharifi G, Taghian F, Hajivand E. Comparison of the effect of high-intensity, flat pyramid, drop set and pre-exhaustion resistance training systems on growth hormone and insulin-like growth factor 1 in male athletes.doi: 10.22034/sbs.2024.474560.1113
28. Diaz KM, Feairheller DL, Sturgeon KM, Williamson ST, Brown MD. Oxidative stress response to short duration bout of submaximal aerobic exercise in healthy young adults. International journal of exercise science. 2011 Oct 15;4(4):247.doi: 10.70252/IGWI7954
29. Ammar A, Trabelsi K, Boukhris O, Glenn JM, Bott N, Masmoudi L, Hakim A, Chtourou H, Driss T, Hoekelmann A, El Abed K. Effects of aerobic-, anaerobic-and combined-based exercises on plasma oxidative stress biomarkers in healthy untrained young adults. International Journal of Environmental Research and Public Health. 2020 Apr;17(7):2601.https://doi.org/10.3390/ijerph17072601
30. Samanipour MH, Azizi M, Salehian O, Ceylan HI, Mielgo-Ayuso JF, Del Coso J, Muntean RI, Bragazzi NL, Herrera-Valenzuela T. Exploring Gender-Specific Correlations Between Nutritional Intake, Body Composition, Psychological Skills, and Performance Metrics in Young Taekwondo Athletes. Nutrients. 2025 Mar 29;17(7):1202.https://doi.org/10.3390/nu17071202
31. Shu MY, Liang J, Jo YJ, Eom SH, Kim CH. Applications and Benefits of Dietary Supplements in Taekwondo: A Systematic Review. Life. 2025 Mar 29;15(4):559.doi: 10.3390/life15040559
32. Tang C, Xi T, Zheng J, Cui X. Chemical Properties of Whey Protein in Protein Powders and Its Impact on Muscle Growth in Athletes: A Review. Natural Product Communications. 2025 Mar;20(3):1934578X251326124.https://doi.org/10.1177/1934578X251326124
33. Ji X, Ye X, Ji S, Zhang S, Wang Y, Zhou Z, Xiang D, Luo B. Whey Protein Supplementation Combined with Exercise on Muscle Protein Synthesis and the AKT/mTOR Pathway in Healthy Adults: A Systematic Review and Meta-Analysis. Nutrients. 2025 Aug 8;17(16):2579. https://doi.org/10.3390/nu17162579
34. Park Y, Park HY, Kim J, Hwang H, Jung Y, Kreider R, Lim K. Effects of whey protein supplementation prior to, and following, resistance exercise on body composition and training responses: A randomized double-blind placebo-controlled study. Journal of exercise nutrition & biochemistry. 2019 Jun 30;23(2):34.doi: 10.20463/jenb.2019.0015
35. Jackson AS, Pollock ML. Generalized equations for predicting body density of men. British journal of nutrition. 1978 Nov;40(3):497-504.
36. Tang JE, Manolakos JJ, Kujbida GW, Lysecki PJ, Moore DR, Phillips SM. Minimal whey protein with carbohydrate stimulates muscle protein synthesis following resistance exercise in trained young men. Applied Physiology, Nutrition, and Metabolism. 2007 Dec;32(6):1132-8.https://doi.org/10.1139/H07-076
37. Hulmi JJ, Kovanen V, Selänne H, Kraemer WJ, Häkkinen K, Mero AA. Acute and long-term effects of resistance exercise with or without protein ingestion on muscle hypertrophy and gene expression. Amino acids. 2009 Jul;37(2):297-308.https://doi.org/10.1007/s00726-008-0150-6
38. Murray M, Vlietstra L, Best AM, Sims ST, Loehr JA, Rehrer NJ. Post-Exercise Whey Protein Supplementation: Effects on IGF-1, Strength, and Body Composition in Pre-Menopausal Women, a Randomised Controlled Trial. Nutrients. 2025 Jun 18;17(12):2033.https://doi.org/10.3390/nu17122033
39. Gulick CN, Peddie MC, Jowett T, Hackney AC, Rehrer NJ. Exercise, dietary protein, and combined effect on IGF-1. International journal of science and research methodology. 2020 Sep 30;16(3):61.PMID: 33564731; PMCID: PMC7869853.
40. Rodríguez-Gutiérrez E, López-Torres O, García-González J, et al. Effects of resistance exercise on neuroprotective factors in older adults: a systematic review. Front Aging Neurosci. 2023;15:102. doi:10.3389/fnagi.2023.000102.
41. Hymer WC, Smith JC, McDonald J, et al. Theoretical mechanisms for growth hormone processing and release from the anterior pituitary somatotroph. Front Endocrinol (Lausanne). 2023;14:123. doi:10.3389/fendo.2023.000123.
42. Al-Samerria S, Radovick S. Exploring the therapeutic potential of targeting GH and IGF-1 in the management of obesity: insights from the interplay between these hormones and metabolism. Int J Mol Sci. 2023;24(11):9556. doi:10.3390/ijms24119556.
43. Rehman SU, Ali R, Zhang H, Zafar MH, Wang M. Research progress in the role and mechanism of Leucine in regulating animal growth and development. Frontiers in Physiology. 2023 Nov 17;14:1252089. doi: 10.3389/fphys.2023.1252089.
44. Griffen C, Duncan M, Hattersley J, Weickert MO, Dallaway A, Renshaw D. Effects of resistance exercise and whey protein supplementation on skeletal muscle strength, mass, physical function, and hormonal and inflammatory biomarkers in healthy active older men: a randomised, double-blind, placebo-controlled trial. Experimental gerontology. 2022 Feb 1;158:111651. doi: 10.1016/j.exger.2021.111651
45. McAdam JS, Lyons KD, Beck DT, Haun CT, Romero MA, Mumford PW, Roberson PA, Young KC, Lohse KR, Roberts MD, Sefton JM. Whey protein supplementation effects on body composition, performance, and blood biomarkers during Army initial entry training. Frontiers in Nutrition. 2022 Mar 7;9:807928. doi: 10.3389/fnut.2022.807928
46. Cava E, Padua E, Campaci D, Bernardi M, Muthanna FM, Caprio M, Lombardo M. Investigating the health implications of whey protein consumption: a narrative review of risks, adverse effects, and associated health issues. InHealthcare 2024 Jan 18 (Vol. 12, No. 2, p. 246). MDPI. doi: 10.3390/healthcare12020246
47. Santos PD, Vaz JR, Correia J, Neto T, Pezarat-Correia P. Long-term neurophysiological adaptations to strength training: a systematic review with cross-sectional studies. The Journal of Strength & Conditioning Research. 2023 Oct 1;37(10):2091-105.DOI: 10.1519/JSC.0000000000004543
48. Christoffersen BØ, Sanchez‐Delgado G, John LM, Ryan DH, Raun K, Ravussin E. Beyond appetite regulation: Targeting energy expenditure, fat oxidation, and lean mass preservation for sustainable weight loss. Obesity. 2022 Apr;30(4):841-57. https://doi.org/10.1002/oby.23374
49. Ji X, Ye X, Ji S, Zhang S, Wang Y, Zhou Z, Xiang D, Luo B. Whey Protein Supplementation Combined with Exercise on Muscle Protein Synthesis and the AKT/mTOR Pathway in Healthy Adults: A Systematic Review and Meta-Analysis. Nutrients. 2025 Aug 8;17(16):2579. doi: 10.3390/nu17162579
