مقایسه اثرات نانوذرات نقره، مس و اکسید روی بر بیان ژن فاکتور نکروز تومور α (TNF-α) در موش Balb/C آلوده به اشریشیا کلی
الموضوعات :مریم محمدخانی 1 , جواد آراسته 2 , صبا طاهری 3
1 - گروه زیست شناسی، واحد تهران مرکزی، دانشگاه آزاد اسلامی، تهران، ایران
2 - دانشکده علوم پایه، گروه زیست شناسی، دانشگاه آزاد اسلامی، واحد تهران مرکزی
3 - دانشکده علوم پایه، دانشگاه آزاد اسلامی واحد اسلامشهر، ایران.
الکلمات المفتاحية: Escherichia coli, نانوذرات, نقره, اکسید روی, مس, فاکتور نکروز توموری آلفا,
ملخص المقالة :
زمینه و اهداف: تعداد افراد مبتلا به باکتری‌های بیماری‌زا در حال افزایش است. آنتی‌بیوتیک‌های رایج به دلیل سازوکارهای ایجاد مقاومت، کارایی خود را از دست می‌دهند. فناوری نانو رویکردی به نام عوامل ضد میکروبی مبتنی بر نانوذرات معرفی کرده است که قادر به مهار رشد باکتری‌ها می‌باشد. مواد و روش‌ها: حداقل غلظت مهارکنندگی (MIC) نانوذرات نقره (Ag) ، اکسید روی (ZnO) و مس (Cu) علیه Escherichia coli ATCC 25922 با استفاده از روش رقت‌سازی در کشت مایع تعیین شد. ۵۴ موش نر نژاد Balb/C به 9 گروه تقسیم شدند، به‌صورت داخل صفاقی با E.coli (CFU/mL 10^8)آلوده شده و با نانوذرات ZnO، Ag و Cu (در دز MIC) یا ایمی‌پنم (گروه کنترل) تحت درمان قرار گرفتند. ۲۴ ساعت پس از درمان، RNA طحال استخراج شد و بیان ژن فاکتور نکروز توموری آلفا (TNF-α) با استفاده از Real-time PCR سنجیده شد. یافته‌ها: MIC نانوذرات Ag برابر ۱۲۵ میکروگرم بر میلی‌لیتر و برای ZnO و Cu برابر ۶۲.۵ میکروگرم بر میلی‌لیتر بود که اثربخشی بهتر نانوذرات ZnO و Cu را نشان داد. بیان ژن TNF-α در گروه‌های دریافت‌کننده ایمی‌پنم و نانوذرات نقره به‌طور معنی‌داری کمتر از گروه‌های دریافت‌کننده ZnO و Cu بود (p < 0.05). نتیجه‌گیری: صرف‌نظر از آنتی‌بیوتیک‌ها، نانوذرات نیز خاصیت ضد میکروبی علیه باکتری‌هایی نظیر E. coli دارند. این ویژگی‌ها آن‌ها را به گزینه‌ای مناسب برای درمان بیماری‌های عفونی تبدیل می‌کند.
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