Effects of dietary protein and Macleaya cordata alkaloid extract supplementation on growth performance, intestinal morphology, immunological responses, serum biochemical metabolites, and the ileal microbiota in broiler chickens

الموضوعات : مجله گیاهان دارویی

Amir Alibemani ¹ , Abbasali Gheisari ² , Yahya Ebrahimnezhad ³ , Naser Maheri-Sis ⁴

1 - Department of Animal Sciences, Shabestar Branch, Islamic Azad University, Shabestar, Iran
2 - Animal Science Research Department, Isfahan Agricultural and Natural Resources Research and Education Center, AREEO, Isfahan, Iran
3 - Department of Animal Sciences, Shabestar Branch, Islamic Azad University, Shabestar, Iran
4 - Department of Animal Sciences, Shabestar Branch, Islamic Azad University, Shabestar, Iran

تاريخ الإرسال : 12 الخميس , شعبان, 1445 تاريخ التأكيد : 21 الأربعاء , ذو القعدة, 1445 تاريخ الإصدار : 06 الأحد , ذو القعدة, 1446

الکلمات المفتاحية: Macleaya cordata extract, Broiler chicken, Low-protein diet, Performance, Intestinal morphology, Immune response.,

ملخص المقالة :

This study investigated the effects of feeding a phytogenic chemical, Macleaya cordata alkaloid extract (MCAE), on growth performance, intestinal morphology, immune responses, biochemical metabolites, and ileal microbiota in broiler chickens fed two levels of crude protein (CP). A total of 560 one-day-old male (Ross 308) broilers were randomly assigned in a 2×4 factorial arrangement, with 8 dietary treatments and 5 replicates per treatment. Two concentrations of dietary CP (100 and 95 % of the recommended requirements) and four inclusion levels of MCAE (0, 180, 360, or 540 mg/kg diet) were applied for 5 weeks. At 35 d, the results showed that supplementation with 540 mg/kg of MCAE in both CP levels resulted in greater (P≤0.05) body weight than the control group. Also, increasing the dosage of MCAE improved broiler chickens' average daily weight gain and feed conversion ratio. The albumin to globulin ratio and relative weights of the spleen and bursa were not affected by dietary CP and MCAE supplementation. The results indicated that 360 mg/kg MCAE supplementation significantly (P<0.05) reduced the heterophil-to-lymphocyte ratio compared to the control group. Increasing levels of MCAE supplementation increased blood cholesterol and triglyceride levels linearly (P<0.05). The ileum villus height: crypt depth ratio in chickens fed low-protein diets was greater than the control diet. The coliform bacteria count of ileum on day 15 was not affected by dietary treatments but increasing the MCAE supplementation level from 0 to 540 mg/kg decreased the coliform count of broiler chickens numerically. When the levels of MCAE supplementation were increased on day 28, antibody titers against Newcastle disease increased numerically. Finally, our findings indicated that supplementing broiler chicken diets with 540 mg/kg MCAE can improve performance and immunological responses.

المصادر:

Abbasi, M.A., Mahdavi, A.H., Samie, A.H. and Jahanian, R. 2014. Effects of different levels of dietary crude protein and threonine on performance, humoral immune responses and intestinal morphology of broiler chicks. Brazilian Journal of Poultry Science,16: 35-44.
Abdelli, N., Sola-Oriol, D. and Francisco Pérez, J. 2021. Phytogenic feed additives in poultry: Achievements, prospective and challenges. Animals, 11(12): 3471.
Afiouni, A., Toghyani, M. and Landy, N. 2023. Performance, morphological responses of thesmall intestine, and humoral immunity of broilers fed oak acorn (Quercus brantii) as a substitution for antibiotic growth promoters. Iranian Journal of Applied Animal Science,13: 321-331.
Ajao, A.M., White, D., Kim, W.K. and Olukosi, O.A. 2022. Partial replacement of soybean meal with canola meal or corn DDGS in low-protein diets supplemented with crystalline amino acids: effect on growth performance, whole-body composition, and litter characteristics. Animals, 12 (19): 2662.
Aljumaah, M.R.,Suliman, G.M., Abdullatif, A.A. and Abudabos, A.M. 2020. Effects of phytobiotic feed additives on growth traits, blood biochemistry, and meat characteristics of broiler chickens exposed to Salmonella typhimurium. Poultry Science,99: 5744-5751.
Aviagen. 2019. Ross 308: Broiler Performance Objectives and Nutrition Specifications. Aviagen Ltd., Newbridge, UK.
Bavarsadi, M., Mahdavi, A.H., Ansari-Mahyari. S. and Jahanian, E. 2017. Effects of different levels of sanguinarine on antioxidant indices, immunological responses, ileal microbial counts and jejunal morphology of laying hens fed diets with different levels of crude protein. Journal of Animal Physiology and Animal Nutrition,101:936-948.
Chen, J., Kang, B., Yao, K., Fu, C.and Zhao, Y. 2019. Effects of dietary Macleaya cordata extract on growth performance, immune responses, antioxidant capacity, and intestinal development in weaned piglets.Journal of Applied Animal Research, 47: 349-356.
Denbow, D.M. 2000. Gastrointestinal anatomy and physiology. Pages in 299-325 inSturkie 's Avian Physiology, 5th ed,: Whittow G.C., (ed), Academic Press.New York, USA.
Ferreira, J.R.de M., Ferraz, P.A., da Silva, A.H., Pugliesi, G. and Gobesso, A.A.de O. 2023. Macleaya cordata extract phytogenic additive: A safe alternative for horses that influences intestinal wall thickness. Livestock Science,275: 105290.
Goodarzi Boroojeni, F., Manner, K. and Zentek, J. 2018. The impacts of Macleaya cordata extract and naringin inclusion in post-weaning piglet diets on performance, nutrient digestibility and intestinal histomorphology. Archives of Animal Nutrition,72: 178-189.
Gudev, D., Popova-Ralcheva, S., Moneva, P., Bonovska, M., Valchev, G. and Valcheva, A. 2004. Effect of supplemental Sangrovit on some biochemical indices and leukocytes phagocytic activity in growing pigs. Archiva Zootehnica,7: 123-134.
Guo, S., Lei, J., Liu, L., Qu, X., Li, P., Liu, X., Guo, Y., Gao, Q., Lan, F., Xiao, B., He, C. and Zou, X. 2021. Effects of Macleaya cordata extract on laying performance, egg quality, and serum indices in Xuefeng black-bone chicken. Poultry Science,100: 101031.
Gross, W.B.and Siegel, P.B. 1993. General principles of stress and welfare. Livestock Handling and Transport, CAB International. Pp. 21-33.
Huang, P., Zhang, Y., Xiao, K., Jiang, F., Wang, H., Tang, D., Liu, D., Liu, B., Liu, Y., He, X., Liu, H., Liu, X., Qing, Z., Liu, C., Huang, J., Ren, Y., Yun, L., Yin, L., Lin, Q., Zeng, C., Su, X., Yuan, J., Lin, L., Hu, N., Cao, H., Huang, S., Guo, Y., Fan, W. and Zeng, J. 2018. The chicken gut metagenome and the modulatory efects of plant-derived benzylisoquinoline alkaloids. Microbiome,6:211.
Hussein, E.O.S., Ahmed, S.H., Abudabos, A.M., Aljumaah, M.R., Alkhlulaifi, M.M., Nassan, M.A., Suliman, G.M., Naiel,M.A.E.andSwelum, A.A. 2020. Effect of antibiotic, phytobiotic and probiotic supplementation on growth, blood indices and intestine health in broiler chicks challenged with Clostridium perfringens. Animals,10(3): 507.
Islam, M.M., Islam, M.N., Sharifuzzaman, and Fakhruzzaman, M. 2014. Isolation and identification of Escherichia coli and Salmonella from poultry litter and feed. International Journal of Natural and Social Sciences,1:1-7.
Jankowski, J., Zduńczyk, Z., Juśkiewicz, J., Kozłowski, K., Lecewicz, A. and Jeroch, H. 2009. Gastrointestinal tract and metabolic response of broilers to diets with the Macleaya cordata alkaloid extract. European Poultry Science,73(2): 95-101.
Karimi, M., Foroudi, F.andAbedini,M.R. 2014. Effect of sangrovit on performance and morphology of small intestine and immune response of broilers. Biosciences Biotechnology Research Asia,11(2):855-861.
Kavyani, A., Zare Shahne, A., Pourreza, J., Jalali, S.M.A., Nikkhah, M. and Landy, N. 2014. Efficiency of different levels of mushroom (Agaricus bisporus) on intestinal morphology and microflora of broiler chickens. Journal of Farm Animal Nutrition and Physiology, 9(1): 23-30.
Kikusato, M., Xue, G., Pastor, A., Niewold, T.A. and Toymizu, M. 2021. Effects of plant-derived isoquinoline alkaloids on growth performance and intestinal function of broiler chickens under heat stress. Poultry Science, 100:957-963.
Khadem, A., Soler, L., Everaert, N.and Niewold, T.A. 2014. Growth promotion in broilers by both oxytetracycline and Macleaya cordata extract is based on their anti-inflammatory properties. British Journal of Nutrition, 112:1110-1118.
Khongthong, S., Faroongsarng, D., Roekngam, N., Nopparat, J., Kraitavin, W., Pastor, A., Steiner, T. and Theapparat Y. 2023. Sanguinarine-based isoquinoline alkaloids modulated the gut-brain axis and enhanced growth performance and gut integrity in natural heat stress broiler chickens. Livestock Science,275: 105297.
Kosina, P., Walterova, D., Ulrichova, J., Lichnovsky, V., Stiborova, M., Rydlova, H., Vicar, J., Krecman, V., Brabec, M.J. and Simanek, V. 2004. Sanguinarine and chelerythrine: assessment of safety on pigs in ninety days feeding experiment. Food and Chemical Toxicology,42:85-91.
Landy, N., Ghalamkari,G., Toghyani, M. and Moattar, F. 2018. Effects of using different forms and levels of Echinacea purpurea L. on immune responses of broiler chicks. African Journal of Immunology Research,5:394-400.
Landy, N. and Kheiri, F. 2023. Effects of hydrolyzed cottonseed protein on growth performances, carcass traits, immunity, microbial and morphological responses of the small intestine and total antioxidant capacity of serum, and small intestine in broiler chickens. Iranian Journal of Applied Animal Science,13:121-132.
Liu, G., Guan, G., Fang, J., Martínez, Y., Chen, S., Bin, P., Duraipandiyan, V., Gong, T., Carine, M., Tossou, B., Al-Dhabi, N.A. and Yin, Y. 2016. Macleaya cordata extract decreased diarrhea score and enhanced intestinal barrier function in growing piglets. BioMed Research International, 1069585.
Liu, Z.Y., Wang,X.L., Ou, S.Q., Hou, D.X. and He, J.H. 2020. Sanguinarine modulate gut microbiome and intestinal morphology to enhance growth performance in broilers. PLOS One,15:e0234920.
Liu, Y., Li, Y., Niu, J., Liu, H., Jiao, N., Huang, L., Jiang, S., Yan, L. and Yang, W. 2022. Effects of dietary Macleaya cordata extract containing isoquinoline alkaloids supplementation as an alternative to antibiotics in the diets on growth performance and liver health of broiler chickens. Frontiers in Veterinary Science,9: 950174.
Lee, K., Kim, J.S., Oh, S.T., Kang, C.W. and An, B.K. 2015. Effects of dietary sanguinarine on growth performance, relative organ weight, cecalmicro flora, serum cholesterol level and meat quality in broiler chickens. Journal of Poultry Science,52:15-22.
Manaa, E.A., Abdel-Latif, M.A., Ibraheim, S.E., Sakr, A., Dawood, M., Albadrani, G.M., El-kott, A.F., Abdel-Daim, M.M. and Shafik, B.M. 2022. Impacts of Macleaya cordata on productive performance, expression of growth-related genes, hematological, and biochemical parameters in turkey. Frontiers in Veterinary Science,9:873951.
Martel, J., Ojcius, D.M., Ko, Y.F. and Young, J.D. 2020. Phytochemicals as prebiotics and biological stress inducers. Trends in Biochemical Sciences,45:462–471.
Mateos, G.G., Jimenez-Moreno, E., Serrano, M.P. and Lazaro,R.P. 2012. Poultry response to high levels of dietary fiber sources varying in physical and chemical characteristics. Journal of Applied Poultry Research,21:156-174.
Mathivanan, R. and Kalaiarasi, K. 2007. Panchagavya and Andrographispanculata as alternatives to antibiotic growth promoters on hematological, serum biochemical parameters and immune status of broilers.Journal of Poultry Science,44: 198-204.
Mirzadeh, M., Rahimi, S., Saeedi M., Mazaheri S., Hatamzade M., Golpour A. and Yakhkeshi S. 2012. Comparison the effect of medicinal plants on growth performance of broiler chickens. 101th Annual Meeting, Poultry Science Association, July 9 -12, Athens, Georgia, USA.
Newton, S.M., Lau, C., Gurcha,S.S., Besra, G.S.and Wright, C.W. 2002. The evaluation of forty-three plant species for in vitroantimycobacterial activities; isolation of active constituents from Psoraleacorylifolia and Sanguinaria Canadensis. Journalof Ethnopharmacology,79: 57-67.
Niu, X., Fan, T., Li, W., Zing, W. and Huang, H. 2012. The anti-inflammatory effects of sanguinarine and its modulation of inflammatory mediators from peritoneal macrophages. European Journal of Pharmacology,689:262-269.
O’Connell, T.X., Horite, T.J. and Kasravi, B. 2005. Understanding and interpreting serum protein electrophoresis. American Family Physician,71: 105-112.
Pickler, L., Beirao, B.C.B., Hayashi, R.M., Durau, J.F., Lourenco, M.C., Caron, L.F.andSantin, E.2013. Effect of sanguinarine in drinking water on Salmonella control and the expression of immune cells in peripheral blood and intestinal mucosa of broilers. Journal of Applied Poultry Research,22: 430-438.
Reansoi, A., Ruangpanit, Y. and Attamangkune, S. 2015. Effect of quaternary benzophenantridine and protopine alkaloids on growth response and gut health of broiler under hot climate management. Proceedings of 53rdKasetsart University Annual Conference, Kasetsart University, Bankok, Thailand.
Santos, R.R., Awati, A., Hil, P.J.R., Tersteeg-Zijderveld, M.H.G., Koolmees, P.A. and Fink-Gremmels, J. 2015. Quantitative histomorphometric analysis of heat-stress-related damage in the small intestines of broiler chickens. Avian Pathology,44: 19-22.
SASInstitute. 2010. SAS®/STAT Software, Release 9.2.SAS Institute, Inc., Cary, NC, USA.
Sneeringer, S., Short, G., MacLachlan, M. and Bowman, M. 2020. Impacts on livestock producers and veterinarians of FDA policies on use of medically important antibiotics in food animal production. Applied EconomicPerspectives and Policy,42: 674-694.
Song, B., He, J., Pan, X., Kong, L., Xiao, C., Keerqin, C. and Song, Z. 2023. Dietary Macleaya cordata extract supplementation improves the growth performance and gut health of broiler chickens with necrotic enteritis. Journal of Animal Science and Biotechnology,14(1): 113.
Teirlynck, E., Bjerrum, L., Eeckhaut, V., Huygebaert, G.,Pasmans, F., Haesebrouck, F., Dewulf, J., Ducatelle, R.R.andImmerseel, F.V. 2009. The cereal type in feed influences gut wall morphology and intestinal immune cell infiltration in broiler chickens. British Journal of Nutrition,102: 1453-1461.
Tschirner, K., Susenbeth, A. and Wolfram, S. 2003. Influence of Sangrovit®supplementation on nitrogen balance and feed intake in growing pigs. In: Vitamine and Zusatzstoffe in der Ernahrung von Mensch und Tier. Pp. 275-279 in Proc. 9thSymp. Jena/Thuringen, Germany.
Uni, Z., Noy, Y.and Sklan, D. 1995. Posthatch changes in morphology and function of the small intestine in heavy and light strain chicks. Poultry Science,74: 1622-1629.
Valenzuela-Grijalva, N.V., Pinelli-Saavedra, A., Muhlia-Almazan, A., Domínguez-Díaz, D. and González-Ríos, H. 2017. Dietary inclusion effects of phytochemicals as growth promoters in animal production. Journal of AnimalScience and Technology,59: 8.
Vieira, S.L., Oyarzabal, O.A., Freitas, D.M., Berres, J., Pena, J.E.M., Torres, C.A.andConeglian, J.L.B. 2008a. Performance of broilers fed diets supplemented with sanguinarine-like alkaloids and organic acid.Journal of Applied Poultry Research, 17: 128-133.
Vrba, J., Hrbac, J., Ulrichova, J.andModriansky, M. 2004. Sanguinarine is a potent inhibitor of oxidative burst in DMSO-differentiated HL-60 cells by a non-redox mechanism. Chemico-Biological Interactions,147: 35-47.
Wang, M., Zhang, J., Huang, X., Liu, Y. and Zeng, J. 2022. Effects of dietary Macleaya cordata extract on growth performance, biochemical indices, and intestinal microbiota of yellow-feathered broilers subjected to chronic heat stress. Animals,12: 2197.
Wang, F., Yin, Y., Yang, M., Chen, J., Fu, C. and Huang, K. 2021. Effects of combined supplementation of Macleaya cordata extract and benzoic acid on the growth performance, immune responses, antioxidant capacity, intestinal morphology, and microbial composition in weaned piglets. Frontiers in Veterinary Science,8: 708597.
Wang, J.P., Yoo, J.S., Kim, H.J., Lee, J.H. and Kim, I.H. 2009. Nutrient digestibility, blood profiles and fecal microbiota are influenced by chitooligosaccharide supplementation of growing pigs. Livestock Science,125:298-303.
Wegmann, T.G. and Smithies, O.A. 1966. Simple hemagglutination system requiring small amounts of red blood cells and antibodies. Transfusion,6:67-73.
Wijtten, P., Langhout, D.and Verstegen, M. 2012. Small intestine development in chicks after hatch and in pigs around the time of weaning and its relation with nutrition: A review.Acta Agriculturae Scandinavica Section A-Animal Science, 62:1-12.
Windisch, W., Schedle, K., Plitzer, C. and Kroismayr, A. 2008. Use of phytogenic products as feed additives for swine and poultry. Journal of Animal Science,86: 140-148.
Yakhkeshi, S., Rahimi, S.and GharibNaseri, K. 2011. The effects of comparison of herbal extracts, antibiotics, probiotics and organic acid on serum lipids, immune response, GIT microbial population, intestinal morphology and performance of broilers.Journal of Medicinal Plants,10: 80-95.
Yang, J., Wang, J., Liu, X. and Fang, X. 1999. Experimental studies on pharmacodynamics effect of Macleaya cordata. J. Chin. Med. Mater. Journal of Chinese Medicinal Materials,22:82-85.
Yin, G., Tang, S., Liu, L., Cao, A., Xie, J.and Zhang, H. 2021. Effects of bile acids on growth performance and lipid metabolism during chronic heat stress in broiler chickens. Animals,11: 630.
Zdunczyk, Z., Gruzauskas, R., Juskiewicz,J., Semaskaite, A., Jankowski, J., Godycka-Klos, I., Jarule, V., Miezeliene, A.andAlencikiene, G. 2010. Growth performance, gastrointestinal tract responses, and meat characteristics of broiler chickens fed a diet containing the natural alkaloid sanguinarine from Macleaya cordata. Journal of Applied Poultry Research, 19:393-400.
Zhang, C., Li, W., Chen, L., Chen, Z., Wang, X., Xu, Q., Zhang, H., Chen, H. and Liu, J. 2022. Oregano oil combined with Macleaya cordata oral solution improves the growth performance and immune response of broilers. Animals,12: 2480.