اثر تعاملی یک دوره تمرین هوازی به همراه مصرف عصاره سیر و استویا بر بیان ژن گیرنده‌های انسولین، شبه- پپتید-گلوکاگن-1 در رت های نر ویستار چاق شده با رژیم پر چرب
الموضوعات :رسول عزیزی 1 , رضا رضایی شیرازی 2 , سیدجواد ضیاءالحق 3 , ندا آقایی بهمن بگلو 4
1 - گروه تربیت بدنی و علوم ورزشی، واحد علی آباد کتول، دانشگاه آزاد اسلامی، علی آباد کتول، ایران
2 - گروه تربیت بدنی، واحد علی آباد کتول، دانشگاه آزاد اسلامی، علی آباد کتول، ایران
3 - گروه تربیت بدنی، دانشکده علوم انسانی، دانشگاه آزاد اسلامی واحد شاهرود، شاهرود
4 - گروه تربیت بدنی و علوم ورزشی، واحد علی آباد کتول، دانشگاه آزاد اسلامی، علی آباد کتول، ایران
الکلمات المفتاحية: ورزش هوازی, عصاره سیر-استویا, بیان ژن انسولین, گلوکاگن, رت ویستار,
ملخص المقالة :
زمینه و هدف: ورزش و عصاره‌های خوراکی در جلوگیری افزایش چاقی و عوارض آن نقش دارد. هدف از این تحقیق بررسی اثر مصرف مکمل تمرین هوازی و مصرف عصاره سیر-استویا بر بیان ژن های انسولین، پپتید-شبه گلوکاگن-1 در رت های ویستاره چاق شده با رژیم پرچرب بود. روش کار: در این مطالعه تجربی، تعداد 33 سر موش نر ویستار با وزن 180 تا 220گرم به طور تصادفی در 7 گروه شامل کنترل سالم، چاق، چاق+سیر، چاق+استویا، چاق+ورزش، چاق+ورزش+سیر، چاق+ورزش+استویا تقسیم شدند. رت ها پس از یک دوره 12-18هفته(تحت پروتکل چاقی)براساس شاخص لی به وزن بالای 310 گرم رسیدند. سپس گروه های تجربی به مدت هشت هفته 40 میلی گرم به ازای هرکیلوگرم وزن بدن مکمل سیر دریافت کردند. پروتکل ورزش با 30دقیقه در روز، 8 متر در دقیقه، براساس برنامه 6جلسه در هفته و به مدت هشت هفته اجرا شد. سطوح پروتئین و میزان بیان ژن با استفاده از کیت الایزا و روش Real Time PCR اندازه‌گیری و داده ها به روش تحلیل واریانس یک طرفه و آزمون تعقیبی توکی در سطح معنی داری p-value0.05). نتیجه گیری: چاقی موجب تغییرات چشمگیر بیان ژن های درگیر در حساسیت انسولین دارد و بنظر میرسد فعالیت بدنی هوازی با و بدون سیر و استویا میتواند بر این تغییرات اثر مثبت داشته باشد.
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