Antihyperuricemic Effects of Turmeric, Ginger, and Black Pepper in Rats
الموضوعات :
Radwa Donia
1
,
Mona Hanfy M.
2
,
Doaa Elsabakhawi
3
,
Ghada Soliman
4
1 - Ph.D in Biochemistry, National Nutrition Institute of Nutrition, Cairo, Egypt
2 - Lecturer Analytical Chemistry, National Nutrition Institute, Cairo, Egypt
3 - Assistant Professor of Nutrition, National Nutrition Institute Cairo, Egypt
4 - Professor of Biochemistry, National Nutrition Institute Cairo, Egypt
الکلمات المفتاحية: Hyperuricemia, Spices, Uric acid level, Lipid profile, Functional foods ,
ملخص المقالة :
Hyperuricemia, characterized by high uric acid levels in blood, can lead to gout and other health issues like type 2 diabetes and hypertension. This study aims to assess the Antihyperuricemic effects of turmeric, ginger, and black pepper powdered spices, individually and in combination, on rats with induced hyperuricemia. The study involved analyzing ginger, turmeric, and black pepper for microbiological safety, aflatoxins, nutritional content, and phytochemical compounds before including them in the rats' diet. Rats were adapted and then divided into six groups, with groups 2-6 induced with hyperuricemia and treated with different combinations of spices or allopurinol as medication for hyperuricemia. The experiment was conducted over a period of 8 weeks, during which body weight and dietary intake were monitored weekly. After the experiment, blood samples were analyzed. Results showed a significant reduction in uric acid, creatinine, and urea levels in the treated group, measuring 3.11 ± 0.29, 1.69 ± 0.11, and 30.51 ± 3.46, respectively, compared to the untreated group, which recorded 5.73 ± 0.27, 3.16 ± 0.19, and 43.81 ± 3.7.Additionally, the lipid profile parameters in the treated group, including total cholesterol, triglycerides, and low-density lipoprotein (LDL), were 87.33 ± 4.1, 102.74 ± 5.0, and 33.59 ± 3.5, respectively. These values were significantly lower than those in the untreated group, which recorded 142.29 ± 6.78, 140.8 ± 4.61, and 85.53 ± 7.9, respectively. Overall, the findings support the use of turmeric, ginger, and black pepper as functional foods in addressing hyperuricemia and associated comorbidities.
1. Kelso E.W., Moore T.L., 2020. Gout. In: Longo D.L., Fauci A.S., Kasper S.L., Hauser S.L., Jameson J.L., Loscalzo J. (Eds.), Harrison's Principles of Internal Medicine. 20th ed., Vol. 2, McGraw-Hill Education.
2. Luo Q., Ding R., Chen L., Bu X., Xiao M., Liu X., Wu Y., Xu J., Tang W., Qiu J., Ding X., Tang X., 2022. The association between spicy food intake and risk of hyperuricemia among Chinese adults. Front Public Health. 10, Article 919347.
3. Danve A., Sehra S.T., Neogi T., 2021. Role of diet in hyperuricemia and gout. Best Practice & Research Clinical Rheumatology. 35(4), 101723.
4. Yadav V., Yadav P., Sahu S., Yadav M., Gupta S.N., 2021. A review of the therapeutic properties and health benefits of ginger (Zingiber officinale). Journal of Current Research and Technology. 9(1). 10.12775/JEHS.2025.79.57812
5. Zhao Y., Li L., Wang J., Zhang Y., Liu J., 2019. Ginger supplementation for the management of hyperuricemia: A systematic review and meta-analysis of randomized controlled trials. Nutrients. 11(7), 1659.
6. Iweala E. J., Uche M. E., Dike E. D., Etumnu L. R., Dokunmu T. M., Oluwapelumi A. E., Okoro B. C., Dania O. E., Adebayo A. H., Ugbogu E. A., 2023. Curcuma longa (turmeric): Ethnomedicinal uses, phytochemistry, pharmacological activities and toxicity profiles—A review. Pharmacological Research – Modern Chinese Medicine. 6, 100222.
Okay, I can help you convert those references to the style you provided. Here's the converted list:
7. Zhang H., Jiang H., Zhao M., Xu Y., Liang J., Ye Y., Chen H., 2022. Treatment of Gout with TCM Using Turmeric and Corn Silk: A Concise Review Article and Pharmacology Network Analysis. Evid.-Based Complement. Altern Med. 2022, 3143733.
8. Lee J., Chae Y., Shin Y., Kim Y., 2020. Chemical Composition and Antioxidant Capacity of Black Pepper Pericarp. Appl Biol. Chem. 63, 1–9.
9. ICMSF: International Commission on Microbiological Specification for Foods, 1998. Microbial Ecology of Foods, Vol. 6: Microorganisms in Food; ICMSF: New York. pp. 356–378.
10. Anonymous, 2004. Enzyme immunoassay for the quantitative analysis of aflatoxin B1. Art. No. R. 1211 R. Biopharm GmbH, Darmstadt, Germany.
11. Anonymous, 2002. Enzyme immunoassay for the quantitative analysis of aflatoxins. Art. No. R. 4701R. Biopharm GmbH: Darmstadt, Germany.
12. Anonymous, 2005. Immuno-affinity column for sample clean-up prior to analysis of aflatoxins. Art. No. R 5001.R. Biopharm GmbH: Darmstadt, Germany.
13. AOAC, 2000. Official Methods of Analysis of the Association of Official Analytical Chemists, 14th ed., AOAC: Washington, D.C.
14. AOAC, 2009. Official Methods of Analysis of the Association of Official Analytical Chemists, 20th ed., AOAC: Washington, D.C.
15. AOAC, 2006. Official Methods of Analysis, 18th ed. [Revised], Association of Official Analytical Chemists: Washington, D.C.
16. Harborne J.B., 1998. Phytochemical Methods, 3rd ed., Chapman and Hall: New York.
17. Kokate C.K., 2001. Pharmacognosy, 16th ed., Nirali Prakasham: Mumbai, India.
18. Kaur C., Kapoor H.C., 2002. Antioxidant activity and total phenolic content of some Asian vegetables. International Journal of Food Science and Technology. 37, 153–161.
19. Piyanete C., Meechai P., Nakbanpotecc W., 2009. Antioxidant activities and phenolic contents of extracts from Salvinia molesta and Eichornia crassipes. Research Journal of Biological Sciences. 4, 1113–1117.
20. Reeves P.G., Nielsen F.H., Fahey G.C. Jr., 1993. AIN-93 purified diets for laboratory rodents: final report of the American Institute of Nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet. The Journal of Nutrition. 123(11), 1939–1951.
21.Tietz N.W., 1990. Clinical Guide to Laboratory Tests. 2nd Edition, W.B. Saunders Co., Philadelphia, 566.
22. Patton G., Crouch S., 1977. Colorimetric Method for the Determination of Serum Urea. Analytical Chemistry. 49, 464-469.
23.Tietz N.W., 1986. Textbook of Clinical Chemistry. W.B. Saunders Co., Philadelphia, 1271-1281.
24.Barham D., Trinder P., 1972. Improved Color Reagent for the Determination of Blood Glucose by the Oxidase System. Analyst. 97, 142-145.
25. Allain C.C., Poon L.S., Chan C.S., Richmond W., Fu P.C., 1974. Enzymatic determination of total serum cholesterol. Clinical Chemistry. 20(4), 470–475.
26. Burstein M., Scholnick H.R., Monfin R., 1970. Rapid method for the isolation of lipoproteins from human serum by precipitation with polyanions. J Lipid Res. 11, 585-595.
27. Lopes-Virella M.F., Stone P., Ellis S., Colwell J.A., 1977. Cholesterol determination in high-density lipoproteins separated by three different methods. Clinical Chemistry. 23(5), 882–884.
28. Levy R.I., 1981. Cholesterol, lipoproteins, apoproteins, and heart disease: present status and future prospects. Clinical Chemistry. 27(5), 653–662.
29. Bucolo G., David H., 1973. Quantitative determination of serum triglycerides by the use of enzymes. Clinical Chemistry. 19(5), 476–482.
30. Drury R.A., Wallington E.A., 1980. Carleton's Histological Techniques. 5th Edition, Oxford University Press, New York, 195.
31. Bailar J.C., Mosteller F., 1992. Medical uses of statistics. Boston, MA: New England Journal Medicine Books.
32.Vázquez-Fresno R., Rosana A.R.R., Sajed T., Onookome-Okome T., Wishart N.A., Wishart D.S., 2019. Herbs and Spices- Biomarkers of Intake Based on Human Intervention Studies – A Systematic Review. Genes & Nutrition. 14, 18.
33. Wang M., Huang W., Xu Y., 2023. Effects of spicy food consumption on overweight/obesity, hypertension and blood lipids in China: a meta-analysis of cross-sectional studies. Nutrition Journal. 22(1), 29.
34. Shobana S., Naidu K.A., Chetana R., 2012. Evaluation of phytochemical composition and antioxidant capacity of selected Indian spices. Journal of Agricultural and Food Chemistry. 60(14), 3738–3744.
35. Prasad S., Tyagi A.K., 2015. Ginger and its constituents: Role in prevention and treatment of gastrointestinal cancer. Gastroenterology Research and Practice. 2015, 142979.
36. Aggarwal B.B., Yuan W., Li S., Gupta S.C., 2013. Curcumin-free turmeric exhibits anti-inflammatory and anticancer activities: Identification of novel components of turmeric. Molecular Nutrition & Food Research. 57(9), 1529–1542.
37. Sahebkar A., 2014. Are curcuminoids effective C-reactive protein-lowering agents in clinical practice? Evidence from a meta-analysis. Phytotherapy Research. 28(5), 633–642.
38. Ali A.M.A., El-Nour M.E.M., Yagi S.M., 2018. Total phenolic and flavonoid contents and antioxidant activity of ginger (Zingiber officinale Rosc.) rhizome, callus and callus treated with some elicitors. Journal of Genetic Engineering & Biotechnology. 16(2), 677–682.
39. Pradeep C.R., Kuttan G., Kuttan R., 2017. Anti-inflammatory activity of extract of the oyster mushroom, Pleurotus ostreatus, through its inhibitory effect on nitric oxide production and the expression of pro-inflammatory cytokines in RAW 264.7 macrophages. Inflammopharmacology. 25(5), 531–537.
40. Meghwal M., Goswami T.K., 2013. Piper nigrum and piperine: An update. Phytotherapy Research. 27(8), 1121–1130.41. 41. Kumar S., Suresh P.K., Vijayababu M.R., 2010. Aronia melanocarpa (Black Chokeberry) reduces ethanol-induced gastric damage via regulation of HSP-70, NF-κB, and MCP-1 signaling. Phytotherapy Research. 24(7), 948–954.
42. Kesarwani K., Gupta R., Mukerjee A., 2013. Bioavailability enhancers of herbal origin: an overview. Asian Pacific Journal of Tropical Biomedicine. 3(4), 253–266.
43. Alghamdi Y., Soliman M.M., Nassan M.A., 2020. Impact of Lesinurad and allopurinol on experimental Hyperuricemia in mice: biochemical, molecular and Immunohistochemical study. BMC Pharmacol Toxicol. 21, 10.
44. Wang C., Pan Y., Zhang Q.Y., Wang F.M., Kong L.D., 2012. Quercetin and allopurinol ameliorate kidney injury in STZ-treated rats with regulation of renal NLRP3 inflammasome activation and lipid accumulation. PloS One. 7(6), e38285.
45. Aggarwal B.B., Harikumar K.B., 2009. Potential therapeutic effects of curcumin, the anti-inflammatory agent, against neurodegenerative, cardiovascular, pulmonary, metabolic, autoimmune and neoplastic diseases. The International Journal of Biochemistry & Cell Biology. 41(1), 40-59.
46. Hewlings S.J., Kalman D.S., 2017. Curcumin: A review of its’ effects on human health. Foods. 6(10), 92.
47. Mashhadi N.S., Ghiasvand R., Askari G., Hariri M., Darvishi L., Mofid M.R., 2013. Anti-oxidative and anti-inflammatory effects of ginger in health and physical activity: review of current evidence. International Journal of Preventive Medicine. 4(Suppl 1), S36.
48. Daily J.W., Yang M., Park S., 2016. Efficacy of turmeric extracts and curcumin for alleviating the symptoms of joint arthritis: a systematic review and meta-analysis of randomized clinical trials. Journal of Medicinal Food. 19(8), 717-729.
49. Johnson R.J., Nakagawa T., Jalal D., Sánchez-Lozada L.G., Kang D.H., Ritz E., 2013. Uric acid and chronic kidney disease: which is chasing which? Nephrology Dialysis Transplantation. 28(9), 2221–2228.
50. Ziga N., Becic F., 2013. Allopurinol effect on values of lipid profile fractions in Hyperuricemic patients diagnosed with metabolic syndrome. Materia Socio-Medica. 25(3), 167–169.
51.He C., Miyazawa T., Abe C., Ueno T., Suzuki M., Mizukami M., Kurihara K., Toda M., 2023. Hypolipidemic and Anti-Inflammatory Effects of Curcuma longa-Derived Bisacurone in High-Fat Diet-Fed Mice. International Journal of Molecular Sciences. 24(11), 9366.
52.Wang D., Zhang L., Huang J., Himabindu K., Tewari D., Horbańczuk J.O., Xu S., Chen Z., Atanasov A.G., 2021. Cardiovascular protective effect of black pepper (Piper nigrum L.) and its major bioactive constituent piperine. Trends in Food Science & Technology. 117, 34-45.
53. Salaramoli S., Mehri S., Yarmohammadi F., Hashemy S.I., Hosseinzadeh H., 2022. The effects of ginger and its constituents in the prevention of metabolic syndrome: A review. Iranian Journal of Basic Medical Sciences. 25(6), 664–674.
54. Hahn K., Kanbay M., Lanaspa M.A., Johnson R.J., Ejaz A.A., 2017. Serum uric acid and acute kidney injury: A mini review. Journal of Advanced Research. 8(5), 529–536.
55. Stamp L.K., Chapman P.T., Palmer S.C., 2019. Allopurinol and urate-lowering therapy in chronic kidney disease. Nature Reviews Nephrology. 15(5), 329–343.
56. Prasad S., Aggarwal B.B., 2011. Turmeric, the Golden Spice: From Traditional Medicine to Modern Medicine. In Herbal Medicine: Biomolecular and Clinical Aspects, 2nd ed.; Benzie, I. F. F., Wachtel-Galor, S., Eds.; CRC Press/Taylor & Francis: Boca Raton, FL.
