Antibacterial activities of green tea extracts on five plant pathogenic bacteria
الموضوعات : Biotechnological Journal of Environmental Microbiologyهادی رهاننده 1 , رویا کریمی پور 2
1 - گروه گیاهپزشکی، واحد رشت، دانشگاه آزاد اسلامی، رشت، ایران
2 - گروه گیاه پزشکی، واحد رشت ، دانشگاه آزاد اسلامی ، رشت ، ایران
الکلمات المفتاحية: Solvent, Green tea, Antibacterial, Aqueous extract, Alcoholic extract, Biological control,
ملخص المقالة :
Tea is one of the medicinal plants that is used all over the world. In the preparation of green tea, the leaves are
dried after harvesting and then used without fermentation. Tea extract has antimicrobial properties, but there
is little information about this. The leaves of three clones of 100, seed hybrid and Assam were collected from
tea gardens, and then the collected leaves were dried. Three solvents; aqueous, methanolic and ethanolic, were
used for extraction. The effect of these extracts on the proliferation of five bacteria Clavibacter michiganensis,
Xanthomonas arboricola, Pseudomonas syringae, Dikeya zeae and Pectobacterium carotovorum was investigated
by the paper disc method by measuring the non-growth halo. The results showed that the highest dilutions
of aqueous, ethanolic and methanolic extracts (50%) were the most effective dilutions on the investigated
bacteria. All extracts had the ability to control bacteria. The extracts obtained from clone 100 showed the highest
inhibition rate on the studied bacteria. Among the extracts and clones, the highest amount of inhibition on
Gram-positive bacteria C. michiganensis was by water extract. On average, aqueous extract showed the most
effect and ethanol extract showed the least effect on bacteria. D. zeae showed the highest resistance against
the extracts. It seems that different factors such as type of solvent, concentration, types of microorganisms are
effective in the inhibition rate of green tea extract.
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