Investigating the Effect of Eugenol on Regulating the Inflammatory Pathways under the Control of miR-223-3p in the Glioblastoma
الموضوعات : Journal of Chemical Health RisksNasrollah Naghdi 1 , Mahsa Asadi 2 , Mehdi Safari 3 , Babak Gholamine 4 , Fakhriyeh Mazalzadeh 5
1 - Department of Pharmacology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
2 - Research Committee of Dezful University of Medical Sciences, Dezful, Iran
3 - Assistant Professor Department of Health in Disasters and Emergencies, School of Public Health and Safety, Shahid Beheshti University of Medical Sciences, Tehran, Iran
4 - Department of Pharmacology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
5 - Nursing and Midwifery School, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
الکلمات المفتاحية: Eugenol, Glioblastoma, miR-223-3p, Marker, Treatment,
ملخص المقالة :
MicroRNA-223 (miR-223) has become a significant regulator in various biological processes, particularly in gliomas and inflammatory responses. Research indicates that miR-223 is crucial in modulating drug resistance in glioma stem cells (GSCs), particularly in response to temozolomide (TMZ) treatment. Elevated levels of miR-223 correlate with increased chemoresistance, linked to the downregulation of PAX6 and activation of the PI3K/AKT signaling pathway. Conversely, overexpression of miR-223 enhances the sensitivity of glioblastoma (GBM) cells to radiation-induced apoptosis, suggesting it is an impending therapeutic target. Additionally, miR-223 is connected with epithelial-mesenchymal transition (EMT) in GBM, promoting invasive properties and correlating with the expression of metalloproteinases and vascular endothelial growth factor (VEGFA). In the context of inflammation, miR-223 regulates immune cell functions by targeting NLRP3, a key component of the inflammasome, thereby influencing neutrophil and macrophage activity. Its role in mitigating acute lung injury and liver failure by inhibiting NET formation further underscores its importance in inflammatory diseases. Overall, miR-223 represents a dual-function molecule with implications in cancer progression and inflammatory responses, highlighting its potential as a biomarker and healing objective in gliomas and related conditions. Overall, the findings suggest that both eugenol and miR-223 play significant roles in glioblastoma biology, offering avenues for further research and potential therapeutic interventions.
1. Schwalfenberg, G. K. Solar radiation and vitamin D: Mitigating environmental factors in autoimmune disease. J Environ Public Health, 2012. https://doi.org/10.1155/2012/619381
2. Meyer, A., Sandler, D. P., Beane Freeman, L. E., Hofmann, J. N., & Parks, C. G. Pesticide exposure and risk of rheumatoid arthritis among licensed male pesticide applicators in the agricultural health study. Environ Health Perspect, 2017; 125:1–7. https://doi.org/10.1289/EHP1013
3. Majithia, V., & Geraci, S. A. Rheumatoid Arthritis: Diagnosis and Management. American Pathogens, 2007; 120: 936–939. https://doi.org/10.1016/j.amjmed.2007.04.005
4. Lee Y. C. (2013). Effect and treatment of chronic pain in inflammatory arthritis. Current rheumatology reports, 15(1), 300. https://doi:10.1007/s11926-012-0300-4
5. Gulati, M., Farah, Z., & Mouyis, M. Clinical features of rheumatoid arthritis. Medicine (United Kingdom),2018; 46: 211–215. https://doi.org/10.1016/j.mpmed.2018.01.008
6. Burska, A. N., Hunt, L., Boissinot, M., Strollo, R., Ryan, B. J., Vital, E.,.Ponchel, F. Autoantibodies to posttranslational modifications in rheumatoid arthritis. Mediators of Inflammation, 2014. https://doi.org/10.1155/2014/492873
7. Jacobson, G. A., Ives, S. J., Narkowicz, C., & Jones, G. Plasma glutathione peroxidase (GSH-Px) concentration is elevated in rheumatoid arthritis: A case-control study. Clinical Rheumatology, 2012; 31 :1543–1547. https://doi.org/10.1007/s10067-012-2046-9
8. Mojzis, J., Varinska, L., Mojzisova, G., Kostova, I., & Mirossay, L.Antiangiogenic effects of flavonoids and chalcones. Pharmacological Research, ;57: 259–265. https://doi.org/10.1016/j.phrs.2008.02.005
9. Ribatti, D.The chick embryo chorioallantoic membrane as an in vivo assay to study antiangiogenesis. Pharmaceuticals, 2010; 3: 482–513. https://doi.org/10.3390/ph3030482
10. Purwanti N, Zehn AS, Pusfitasari ED, Khalid N, Febrianto EY, Mardjan SS, et al. Emulsion stability of clove oil in chitosan and sodium alginate matrix. Int J Food Prop 2018;21:566–81. https://doi.org/10.1080/10942912.2018.1454946.
11. Yaghoubi, A., Ghojazadeh, M., Abolhasani, S., Alikhah, H., & Khaki-Khatibi, F. Correlation of Serum Levels of Vitronectin, Malondialdehyde and Hs-CRP With Disease Severity in Coronary Artery Disease. Journal of Cardiovascular and Thoracic Research, 2015; 7 : 113–117. https://doi.org/10.15171/jcvtr.2015.24
12. Liu, Y., Wang, S., Zhang, R., Lan, W., & Qin, W.Development of poly(Lactic acid)/chitosan fibers loaded with essential oil for antimicrobial applications. Nanomaterials, 2017;7. https://doi.org/10.3390/nano7070194
13. Brown, P. M., Pratt, A. G., & Isaacs, J. D. Mechanism of action of methotrexate in rheumatoid arthritis, and the search for biomarkers. Nature Reviews Rheumatology, 2016;12: 731–742. https://doi.org/10.1038/nrrheum.2016.175
14. Cronstein, B. N. Molecular therapeutics: Methotrexate and its mechanism of action. Arthritis and Rheumatism, 1996; 39: 1951–1960. https://doi.org/10.1002/art.1780391203
15. Vasanthi, P., Nalini, G., & Rajasekhar, G. Role of tumor necrosis factor-alpha in rheumatoid arthritis : a review. APLAR Journal of Rheumatology, 2007;270–274. https://doi.org/10.1111/j.1479-8077.2007.00305.x
16. Mieliauskaite, D., Venalis, P., Dumalakiene, I., Venalis, A., & Distler, J. Relationship between serum levels of TGF-β1 and clinical parameters in patients with rheumatoid arthritis and Sjgren’s syndrome secondary to rheumatoid arthritis. Autoimmunity, 2009;42: 356–358. https://doi.org/10.1080/08916930902831977
17. Gong, B. J., Ratkay, L. G., Waterfield, J. D., & Clark-lewis, I. ( MCP-1 ) Inhibits Arthritis in the MRL- lpr Mouse Model. J Exp Med, 1997;186: 1–7. http://doi:10.1084/jem.186.1.131
18. Ng, T. H. S., Britton, G. J., Hill, E. V, Verhagen, J., Burton, B. R., & Wraith, D. C. Regulation of adaptive immunity ; the role of interleukin-10. Front. Immunol, 2013; 1–13. https://doi.org/10.3389/fimmu.2013.00129
19. Wang, K., Zhang, D., Liu, Y., Wang, X., Zhao, J., Sun, T., … Pathak, J. L.Traditional Chinese medicine formula Bi-Qi capsule alleviates rheumatoid arthritis- induced inflammation , synovial hyperplasia , and cartilage destruction in rats. Arthritis Res Ther, 2018;1–12. http://doi: 10.1186/s13075-018-1547-6.
20. Cutolo, M., Otsa, K., Uprus, M., Paolino, S., & Seriolo, B.Vitamin D in rheumatoid arthritis. Autoimmunity Reviews, 2007; 7: 59–64. https://doi.org/10.1016/j.autrev.2007.07.001
21. Garg, N., Abdel-Aziz, S. M., & Aeron, A. Microbes in food and health. Microbes in Food and Health, 2016;1–362. https://doi.org/10.1007/978-3-319-25277-3.
22. Yogalakshmi, B., Viswanathan, P., & Anuradha, C. V. Investigation of antioxidant, anti-inflammatory and DNA-protective properties of eugenol in thioacetamide-induced liver injury in rats. Toxicology, 2010; 268: 204–212. https://doi.org/10.1016/J.TOX.2009.12.018
23. Magyari, L., Varszegi, D., Kovesdi, E., Sarlos, P., Farago, B., Javorhazy, A., … Melegh, B. Interleukins and interleukin receptors in rheumatoid arthritis: Research, diagnostics and clinical implications. World Journal of Orthopaedics, 2014; 5: 516–536. https://doi.org/10.5312/wjo.v5.i4.516
24. Quinonez-Flores, C. M., Gonzalez-Chavez, S. A., Del Rio Najera, D., & Pacheco-Tena, C. Oxidative Stress Relevance in the Pathogenesis of the Rheumatoid Arthritis: A Systematic Review. BioMed Research International, 2016;2. https://doi.org/10.1155/2016/6097417
25. Sathishkumar, K., Gao, X., Raghavamenon, A. C., Murthy, S. N., Kadowitz, P. J., & Uppu, R. M. Free Radicals and Antioxidant Protocols, 2010;610: 51–61. https://doi.org/10.1007/978-1-60327-029-8
26. Fouad, A. A., & Yacoubi, M. T. Mechanisms Underlying the Protective Effect of Eugenol in Rats with Acute Doxorubicin Cardiotoxicity. Arch. Pharm. Res, 2011;34: 821–828. https://doi.org/10.1007/s12272-011-0516-2
27. Ediz, L., Hiz, O., Ozkol, H., Gulcu, E., Toprak, M., & Ceylan, M. F. Relationship between anti-CCP antibodies and oxidant and anti-oxidant activity in patients with rheumatoid arthritis. International Journal of Medical Sciences, 2011;8: 139–147. https://doi.org/10.7150/ijms.8.139
28. Lee, J. C., Espéli, M., Anderson, C. A., Linterman, M. A., Pocock, J. M., Williams, N. J., … Smith, K. G. C. Human SNP links differential outcomes in inflammatory and infectious disease to a FOXO3-regulated pathway. Cell, 2013;155: 57–69. https://doi.org/10.1016/j.cell.2013.08.034
29. Fatah, W. M. E. A., Atef, L., Mohamed, Y., Refaat, D., El-raof, A. M. M. A., & Eissa, F. Study of Serum Monocyte Chemoattractant Protein-1AS A Marker in Rheumatoid Arthritis. Egyptian Journal of Hospital Medicine.2014;56, 321–332. http://DOI: 10.12816/0005579
30. Yeh, J.L., Hsu, J.H., Hong, Y.S., Wu, J.R., Liang, J.C., Wu, B.N., Chen, I.J., Liou, S.F., 2011. Eugenolol and glyceryl-isoeugenol suppress LPS-induced iNOS expression by down-regulating NF-κB and AP-1 through inhibition of MAPKs and Akt/IκBα signaling pathways in macrophages. Int. J. Immunopathol. Pharmacol. 24, 345–356. https://doi.org/10.1177/039463201102400208
