همبستگی شیموتایپ‌های Ramalina farinacea با عوامل اکولوژیکی در ایران با استفاده از LC-ESI-MS/MS
الموضوعات :
مریم شریفی
1
,
ایرج مهرگان
2
,
محمد سهرابی
3
,
کامبیز لاریجانی
4
,
هری سیپمن
5
1 - دانشگاه آزاد واحد علوم تحقیقات تهران
2 - گروه زیست شناسی- واحد علوم و تحقیقات
3 - گروه زیست فناوری صنعتی و محیط زیست، پژوهشکده زیست فناوری، سازمان پژوهش های علمی و صنعتی ایران، تهران، ایران
4 - گروه زیست شناسی، دانشکده علوم وفناوری‌های همگرا، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران
5 - باغ و موزه گیاه شناسی برلین- داهلم، آلمان
الکلمات المفتاحية: ارتفاع, پروفیل شیمیایی, جهت شیب, شیموتایپ, کروماتوگرافی مایع-طیف‌سنجی جرمی, گونه Ramalina farinacea,
ملخص المقالة :
در مطالعه حاضر، خواص فیتوشیمیایی را مشخص کردیم که به طور خاص در پنج اکوتیپ متمایز گلسنگی Ramalina farinacea از ایران گلستان: کردکوی و گرگان، مازندران: فریم و گلوگاه ، سمنان: شاهرود، منطقه کالپوش مرتبط بودند تا تنوع شیموتایپ های درون گونه‌ها تشخیص داده شوند. تفاوت‌های کیفی بین پروفیل‌های شیمیایی با استفاده از کروماتوگرافی مایع-طیف‌سنجی جرمی (LC-ESI-MS/MS) در چند مرحله بررسی گردید. بر اساس نتایج LC-ESI-MS/MS، مواد شیمیایی زیر شناسایی شدند: متابولیت‌های (1) Atranol، (2) Divaric acid، (3) Evernic acid، (4) Ramalinaic acid، (5) Squamatic acid، (6) Sekikaic acid، (7) 4’-0-Methyl norsekikaic acid، (8) Glomellonic acid، (9) Conprotocetraric acid، (10) Usnic acid، (11) Nephrosterinic acid، (12) Dihydroxyoctadecenoic acid، (13) Hypoconstictic acid و دو ماده نا‌شناخته. آنالیزهاي آماري با استفاده از نرم‌افزار SPSS به روش Ward بر پایه صفات کمی و کیفی صورت گرفت که دندروگرام با دو خوشه اصلی ایجاد شد و نزدیکی آرایه‌هاي فریم و گلوگاه باهم در یک زیر خوشه و در زیر خوشه دیگر گرگان، در خوشه‌ی دیگر هم نزدیکی شاهرود و کردکوی کاملاً مشهود است. همچنین ضرایب همبستگی صفات مورد مطالعه، همبستگی مثبت جهت شیب، ارتفاع، رطوبت، بیشینه و میانگین دما با شیموتایپ‌ها را نشان داده که میتواند تفاوت‌هایی که در پروفیل‌های شیمیایی اکوتیپ‌های گلسنگ مشاهده می‌شود را توجیه نماید.
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