Modified Glass Carbon Electrode (GCE) Electropolymerized Polypyrrole Nanofibers with Hemoglobin (Hb) Film as a Unique Biosensor for Nitrite Determination
الموضوعات : فصلنامه نانوساختارهای اپتوالکترونیکی
1 - Department of Chemistry, Marvdasht Islamic Azad University, Fars, Iran
الکلمات المفتاحية: electrochemical sensor, biosensor, Hemoglobin, Polypyrrole Nanofibers, Nitrite,
ملخص المقالة :
Abstract: In this study, we were investigated behavior the electrochemical reduction
of nitrite at a hemoglobin (Hb) immobilized on glass carbon electrode (GCE) containing
polypyrol nanofiber (ppy) films. Polypyrrole (PPy) nanofibers have been constructed on
GCE applying electrochemical technique, and can to deposit diverse polymers on
miniaturized electrodes with this common method. The structure of the acquired
electrode and the electrochemical behavior were characterized using Scanning Electron
Microscopy (SEM), Cyclic Voltammetry (CV), and Linear Sweep Voltammetry (LSV).
The immobilized Hb showed an irreversible reduction peak with a formal potential of –
0.72 versus Ag/AgCl, in 0.1M pH 4.5 acetate buffer. The immobilized Hb exhibited
remarkable electrocatalytic activity for the reduction of nitrite. Under the optimal
condition the nitrite concentrations range 2.0×10−4 to 46.0×10−4 M with a detection limit
of 6.2×10−5 M (at S/N=3). The resulting biosensor has been successfully applied to the
determination of nitrite in water samples. The produced modified electrode for nitrite
determination has been explored within the presence of the interfering substances
including a number of common ions and cations, demonstrating great selectivity.
REFERENCES
[1] M. R. Majidi, A. Saadatirad, and E. Alipour, پgPencil lead electrode modified with hemoglobin film as a novel biosensor for nitrite determination,پh Electroanalysis, vol. 25, no. 7, pp. 1742.1750, 2013.
[2] H. Khodaei et al., پgDetermination and validation of simultaneous derivatization and dispersive liquidپ]liquid microextraction method for analysis of nitrate and nitrite contents as nitrate ions in onion and potato samples,پh Sep. Sci. Plus.
[3] R. Jamaluddin, R. Z. Akhmar, L. Yook Heng, L. L. Tan, and K. F. Chong, پgElectrochemical biosensor for nitrite based on polyacrylic-graphene composite film with covalently immobilized hemoglobin,پh Sensors, vol. 18, no. 5, p. 1343, 2018.
[4] H.-H. Deng et al., پgSensitive and selective nitrite assay based on fluorescent gold nanoclusters and Fe2+/Fe3+ redox reaction,پh Food Chem., vol. 317, p. 126456, 2020.
[5] L. I. U. Hong-Ying et al., پgPrussian Blue Analogue of Copper-Cobalt
88 * Journal of Optoelectronical Nanostructures Spring 2020 / Vol. 5, No. 2
Decorated with Multi-Walled Carbon Nanotubes Based Electrochemical Sensor for Sensitive Determination of Nitrite in Food Samples,پh Chinese J. Anal. Chem., vol. 47, no. 6, pp. e19066.e19072, 2019.
[6] S. Joint FAO/WHO Expert Committee on Food Additives (2002 : Geneva, W. H. Organization, and F. and A. O. of the U. Nations, پgEvaluation of certain food additives : fifty-ninth report of the Joint FAO/WHO Expert Committee on Food Additives.پh World Health Organization, Geneva PP - Geneva, 2002.
[7] I. Joint FAO/WHO Expert Committee on Food Additives (1995 : Rome, W. H. Organization, and F. and A. O. of the U. Nations, پgEvaluation of certain food additives and contaminants : forty-fourth report of the Joint FAO/WHO Expert Committee on Food Additives.پh World Health Organization, Geneva PP - Geneva, 1995.
[8] R. Roohparvar, T. Shamspur, A. Mostafavi, and H. Bagheri, پgIndirect ultra-trace determination of nitrate and nitrite in food samples by in-syringe liquid microextraction and electrothermal atomic absorption spectrometry,پh Microchem. J., vol. 142, pp. 135.139, 2018, doi: https://doi.org/10.1016/j.microc.2018.06.027.
[9] G. Kumar, S. Mukherjee, S. Kumar, and R. Patnaik, پgRapid Determination of Nitrate in Brain Regions and Cerebrospinal Fluid of Transient Bilateral Common Carotid Artery Occlusion Rat Model by HPLC.UV,پh Proc. Natl. Acad. Sci. India Sect. A Phys. Sci., pp. 1.8, 2020.
[10] M. da Silva, A. V. F. Sako, G. A. Micke, and L. Vitali, پgA rapid method for simultaneous determination of nitrate, nitrite and thiocyanate in milk by CZE-UV using quaternary ammonium chitosan as electroosmotic flow inverter,پh J. Food Compos. Anal., vol. 88, p. 103455, 2020.
[11] L. Moravsky, P. Tro.ka, M. Klas, M. Masar, and .. Matej.ik, پgDetermination of nitrites and nitrates in plasmaپ]activated deionized water by microchip capillary electrophoresis,پh Contrib. to Plasma Phys., p. e202000014.
[12] K. H. Al-Sowdani and M. T. K. Al-Balaawi, پgLab.Built semi-automated Stop.flow System for Spectrophotometric Determination of Nitrite in Different water Samples,پh in Journal of Physics: Conference Series, 2019, vol. 1294, no. 7, p. 72010.
[13] D. Shan, G. Cheng, D. Zhu, H. Xue, S. Cosnier, and S. Ding, پgDirect electrochemistry of hemoglobin in poly (acrylonitrile-co-acrylic acid) and its catalysis to H2O2,پh Sensors Actuators B Chem., vol. 137, no. 1, pp. 259.265, 2009.
Modified Glass Carbon Electrode (GCE) Electropolymerized Polypyrrole Nanofibers پc * 89
[14] P. Liu, X. H. Zhang, L. J. Feng, H. Y. Xiong, and S. F. Wang, پgDirect electrochemistry of hemoglobin on graphene nanosheet-based modified electrode and its electrocatalysis to nitrite,پh Am. J. Biomed. Sci, vol. 3, no. 1, pp. 69.76, 2011.
[15] Y. Ding, Y. Wang, and Y. Lei, پgDirect electrochemistry and electrocatalysis of novel single-walled carbon nanotubes.hemoglobin composite microbelts.towards the development of sensitive and mediator-free biosensor,پh Biosens. Bioelectron., vol. 26, no. 2, pp. 390.397, 2010.
[16] S. Liu and H. Ju, پgNitrite reduction and detection at a carbon paste electrode containing hemoglobin and colloidal gold,پh Analyst, vol. 128, no. 12, pp. 1420.1424, 2003.
[17] C. Fan, X. Chen, G. Li, J. Zhu, D. Zhu, and H. Scheer, پgDirect electrochemical characterization of the interaction between haemoglobin and nitric oxide,پh Phys. Chem. Chem. Phys., vol. 2, no. 19, pp. 4409.4413, 2000.
[18] A. J. Bard and L. R. Faulkner, پgFundamentals and applications,پh Electrochem. Methods, vol. 2, no. 482, pp. 580.632, 2001.
[19] X. Han, W. Huang, J. Jia, S. Dong, and E. Wang, پgDirect electrochemistry of hemoglobin in egg.phosphatidylcholine films and its catalysis to H2O2,پh Biosens. Bioelectron., vol. 17, no. 9, pp. 741.746, 2002.
