Protective effects of vitamin B12 on testicular torsion/detorsion injury in rat
الموضوعات :
Rahim Beheshti
1
,
Ehsan Aghamohammadian
2
1 - Department of clinical sciences, Veterinary and agriculture faculty, Shabestar branch, Islamic Azad University.
2 - Department of clinical sciences, Veterinary and agriculture faculty, Shabestar branch, Islamic Azad University.
الکلمات المفتاحية: Vit B12, Testis torsion, Ischemia/reperfusion, Oxidative injury,
ملخص المقالة :
Torsion of the testis is a urologic emergency in males, which causes the interruption of the arterial blood flow of the testis; it can lead to ischemia in the testicular tissue that an important cause of male infertility. The goal of this work was to evaluate the effects of Vit B12 on testis torsion injury after torsion/detorsion. Thirty- five male Wistar rats were randomly divided into five groups containing 8 rats per group including sham, ischemia, ischemia + Vit B12 (4 mg/kg i.p), ischemia / reperfusion and ischemia / reperfusion + Vit B12 (4 mg/kg i.p). Three hours of torsion and 3 hr. of detorsion were created in the both testes. After 3 hr. of detorsion, testis tissues were collected to evaluate glutathione peroxidase (GPx), superoxide dismutase (SOD), malondialdehyde (MDA) concentrations for oxidative stress, and nuclear factor kappa B (NF-κB) expression. There was statistically significant difference in all parameters [SOD (p < 0.001), GPx (p< 0.001), MDA (p < 0.001)], except NF-κB expression when the experimental groups compared to sham group. Tissue GPx and SOD levels were significantly higher, while MDA level was significantly lower in the ischemia / reperfusion + Vit B12 group than in the ischemia / reperfusion group. This study showed that Vit B12 can maintain testis structure against ischemia reperfusion injury. Moreover, Vit B12 attenuated inflammation and related oxidative stress and also helped to preserve testis function following IR damage.
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