Anti-inflammatory and Wound Healing Properties of Curcuma amada and Oregano Essential Oil Derived Hydrogel on 𝘞𝘪𝘴𝘵𝘢𝘳 𝘈𝘭𝘣𝘪𝘯𝘰 Rats
الموضوعات :Ramasamy Mahadevi 1 , Muthugoundar Subramanian Shivakumar 2 , Devarajan Natarajan 3 , Singaravel Sengottuvelu 4 , Ramamoorthy Kavitha 5
1 - Department of Biotechnology, Periyar University, Salem 636011, Tamil Nadu, India
2 - Department of Biotechnology, Periyar University, Salem 636011, Tamil Nadu, India
3 - Department of Biotechnology, Periyar University, Salem 636011, Tamil Nadu, India
4 - Department of Pharmacology, Nandha College of Pharmacy, Erode 638052, Tamil Nadu, India
5 - Department of Biotechnology, Periyar University, Salem 636011, Tamil Nadu, India
الکلمات المفتاحية: Carbopol 934, Essential oil, Herbal hydrogel, Inflammation, Rhizome, Sodium alginate,
ملخص المقالة :
The development of herbal gels for the treating wounds and inflammation is increasing attention to researchers for finding alternatives to conventional drugs with minimal side effects. In the present study, the herbal gel was prepared using Curcuma amada rhizome extract, a combination of oregano oil extracted from Origanum vulgare leaves, and either of three polymers namely carbopol 934, sodium alginate, and sodium carboxymethyl cellulose. Rheological parameters for prepared herbal gel were tested for pH, viscosity, and spreadability. The in-vivo excision wound healing and anti-inflammatory studies were performed on three month-old-male Wistar rats. The results show the best wound healing potential found in herbal formulations containing carbopol 934. This formulation showed good viscosity (65 cPs × 103), spreadability (25 gm × cm sec-1), in-vitro release (100%) and significant (350.97±0.27 mm2) wound contraction compared to a positive control (neomycin sulfate). The results show the highest anti-inflammatory activity in rats group treated with 200 mg kg-1 of methanol extract of C.amada compared to control (indomethacin). The gel was prepared using methanol extract of C.amada, oregano oil, and carbopol 934 which are excellent drug candidates for treating wounds and inflammation.
. Han G., Ceilley R., 2017. Chronic wound healing: a review of current management and treatments. Adv Ther. 34, 599-610.
2. Abdel Halim M.B., Eid H.H., Deeb K.S., 2024. The study of wound healing activity of Thespesia populnea L. bark, an approach for accelerating healing through nanoparticles and isolation of main active constituents. BMC Complement Med Ther. 24(85), 1- 16.
3. Thakur R., Jain N., Pathak R., Sandhu S.S., 2011. Practices in wound healing studies of plants. Evid. 1–17.
4. Zahid M., Lodhi M., Afzal A., Rehan Z.A., Mehmood M., Javed T., Shabbir R., Siuta D., Althobaiti F., Dessok E.S., 2021. Development of Hydrogels with the Incorporation of Raphanus sativus L. Seed Extract in Sodium Alginate for Wound-Healing Application. Gels.7, 107.
5. Nia Yuniarsih, Himyatul Hidayah, Neni Sri Gunarti, Anggun Hari Kusumawati, Farhamzah Farhamzah, Asman Sadino, Maulana Yusuf Alkandahri, 2023. Evaluation of Wound-Healing Activity of Hydrogel Extract of Sansevieria trifasciata Leaves (Asparagaceae). Hindawi Adv Pharma Pharma Sci. 23(1), 1- 10.
6. Ali A., Garg P., Goyal R., Kaur G., Li X., Negi P., Valis M., Kuca K., Kulshrestha S., 2021. A Novel Herbal Hydrogel Formulation of Moringa oleifera for Wound Healing. Plants. 10, 25.
7. El-Sherbeni A., Negm W.A., 2023. The wound healing effect of botanicals and pure natural substances used in in vivo models. Inflam Pharmacology. 31, 755–772.
8. Suhail M., Wu P.C., Minhas M.U., 2020. Using carbomer-based hydrogels for control the release rate of diclofenac sodium: preparation and in vitro evaluation. Pharmaceuticals. 13, 1-17.
9. Cai K., Liu Y., Yue Y., Liu Y., Guo F., 2023. Essential Oil Nanoemulsion Hydrogel with Anti-Biofilm Activity for the Treatment of Infected Wounds. Polymers. 15, 1- 6.
10. Erten F., Tuzcu M., Orhan C., Ozercan I.H., Balci T.A., Tuzcu Z., Juturu V., Sahin K., 2019. Mango ginger supplementation may protect bone damage induced by methotrexate in rats. Acta Poloniae Pharmaceutica. 76, 305–312.
11. Yazici E., Sahin E., Sahin N., Tuzcu M., Sahin K., Orhan C., 2023. Mango ginger (Curcuma amada Roxb.) may alleviate the effect of high-fat diet/streptozotocin-induced diabetes by activation of the GSK-3β/Fyn/Nrf2 pathway. Food Sci Nutr. 4, 6041-6051.
12. Negut I., Valentina G., Alexandru M.G., 2018. Treatment Strategies for Infected Wounds. Molecules. 23, 9.
13. Lopes A.I., Pintado M.M., Tavaria F.K., 2024. Plant-Based Films and Hydrogels for Wound Healing. Microorganisms. 21.
14. Wayal V., NV T., 2022. Assessment of wound healing activity of potent herbal extracts gel in albino Wistar rat. Asian J Pharma Clinical Res. 15, 109-113.
15. Islam M.T., Rodriguez-Hornedo N., Ciotti S., Ackermann C., 2004. Rheological characterization of topical carbomer gels neutralized to different Ph. Pharm Res. 21, 1192-1199.
16. Laferriere C.A., Pang D.S., 2020. Review of Intraperitoneal Injection of Sodium Pentobarbital as a Method of Euthanasia in Laboratory Rodents. J Amer Asso Lab Animal Sci. 59, 254–63.
17. OECD, Test no. 402: acute dermal toxicity, OECD guidelines for the testing of chemicals, Section 4, Paris: OECD Publishing. 2017.
18. Taghavifar S., Afroughi F., SaadatiKeyvan M., 2020. Curcumin nanoparticles improved diabetic wounds infected with methicillin-resistant Staphylococcus aureus sensitized with hamlet. Int J Lower Extremity Wounds. 1– 13.
19. Li L.S., Chiroma S.M., Hashim T., Adam S.K., Mohd Moklas M.A., Yusuf Z., Rahman S.A., 2020. Antioxidant and anti-inflammatory properties of Erythroxylum cuneatum alkaloid leaf extract. Heliyon. 28 (6) 1-6.
20. Kola-Mustapha A.T., Yohanna K.A., Ghazali Y.O., Ayotunde H.T., 2020. Design formulation and evaluation of Chasmanthera dependents Hochst and Chenopodium ambrosioides Linn based gel for its analgesic and anti-inflammatory activities, Heliyon. 6(9), 1-8.
21. Panigrahi L., Ghosal S.K., Pattnaik S., Maharana L., Barik B.B., 2006. Effect of permeation enhancers on the release and permeation kinetics of lincomycin hydrochloride gel formulations through mouse skin. Indian J Pharm Sci. 68, 11.
22. Sugumar V., Hayyan M., Madhavan P., Wong W.F., Looi C.Y., 2023. Current Development of Chemical Penetration Enhancers for Transdermal Insulin Delivery. Biomedicines. 11(3),1-25.
23. Lauffer M.A., 1961. Theory of diffusion in gels. Biophysical J. 1, 205-13.
24. Caglar Emre, Karaotmarli, Gokce, Ustundag Okur Neslihan, 2023. Preparation and Characterization of Carbopol Based Hydrogels Containing Dexpanthenol. J Fac Pharm Ankara. 47, 770-783.
25. Noohinasim, Inavolusriramsandeep, Sujatamohanty, 2022. Plant derived natural products for drug discovery: current approaches and prospects. Nucleus. 65, 399-411.
26. Rajasekaran Aiyalu, Govindarjan Arulkumaran, Ramasamy Arivukkarasu, 2016. Formulation and evaluation of topical herbal gel for the treatment of arthritis in animal model. Bra J Pharma Sci. 52, 493-507.
27. Soha El-Masry M., Sally Helmy A., 2020. Hydrogel-based matrices for controlled drug delivery of etamsylate: Prediction of in-vivo plasma profiles. Saudi Pharma J. 28, 1704–1718.
28. Li C., Liu C., Liu J., Fang L., 2011. Correlation between rheological properties, in vitro release, and percutaneous permeation of tetrahydropalmatine, Pharm Sci Tech. 12, 1002–1010.
29. Ananth K.V., Asad M., Prem Kumar N., Asdaq S.M., Rao G.S., 2010. Evaluation of Wound Healing Potential of Bauhinia purpurea Leaf Extracts in Rats. Indian J Pharm Sci. 72, 122-7.
30. Chinko B.C., Precious-Abraham A.D., 2024. Wound healing activity of hydromethanolic Dioscorea bulbifera extract on male Wistar rat excision wound models. Pharma Res Modern Chinese Medicine.11, 1-6.
31. Pammi S.V.N., Padavala, Kumar V., Karumuri S., Kommavari C.S., Shaik M., Kolapalli V., Lakshmi L., 2023. Wound healing synergy in Wistar albino rats via green synthesized nanoparticles and topical antibiotic neomycin. Open Nano. 11 (1) 1-12.
32. Mujumdar A.M., Naik D.G., Dandge C.N., Puntambekar H.M., 2000. Anti-inflammatory activity of Curcuma amada roxb. In albino rats. Ind J Pharma. 32, 375-377.
33. Rajasekaran A., Arulkumaran G., Arivukkarasu R., 2014. Acute and sub-chronic toxicity study of methanol leaf extract of Cardiospermum halicacabum L and Vitex negundo L in rats. Pharmacognosy Comm. 5, 51-57.
34. Karatas A., Orhan C., Tuzcu, Sahin N., Ozercan I.H., Koca S.S., Juturu V., Sahin K., 2020. Mango ginger (curcuma amada) inhibits collagen-induced arthritis by modulating inflammatory cytokine levels in rats, Turk J Med Sci. 50, 2040-2047.
