اثرات افزودن سطوح مختلف سلنیوم آلی بر پاسخ سیستم ایمنی و بیان ژن های اینترفرون گاما، اینترلوکین 2 و 6 در موش‌های تحت تنش گرمایی
الموضوعات :
1 - گروه علوم دامی واحد علوم و تحقیقات
الکلمات المفتاحية: سلنیوم آلی, اینترفرون گاما, اینترلوکین2, اینترلوکین6, پاسخ سیستم ایمنی, موش,
ملخص المقالة :
دمای بالای محیط سبب ایجاد تنش اکسیداتیو در بدن حیوانات می شود. سلنیوم ماده معدنی کمیابی است که در شرایط تنش بر سلامتی و عملکرد بدن اثر دارد. مطالعات محدودی در زمینه اثر دوزهای مختلف سلنیوم در شرایط تنش گرمایی طولانی مدت بر پاسخ سیستم ایمنی و بیان ژن‌های مرتبط با التهاب وجود دارد. پژوهش کنونی به منظور تعیین اثرات دوزهای مختلف سلنیوم از مکمل سلنیوم-متیونین بر پاسخ سیستم ایمنی و بیان ژن‌های اینترفرون گاما، اینترلوکین 2 و 6 انجام شد. 25 سر موش صحرایی ماده به طور تصادفی به پنج گروه و هر گروه پنج سر موش تقسیم شدند. تعداد 5 سر موش در کل دوره آزمایش در دمای استاندارد و بقیه موش ها در تنش گرمایی قرار داده شدند. موش های گروه کنترل منفی (بدون تنش گرمایی) و کنترل مثبت (تنش گرمایی) پلت استاندارد بدون افزودنی و سه گروه دیگر به ترتیب پلت استاندارد به اضافه 0/15، 0/30 و 0/45 میلی گرم در کیلوگرم سلنیوم از مکمل سلنیوم-متیونین به مدت 30 روز دریافت کردند. افزودن مکمل سلنیوم به جیره سبب افزایش بیان ژن اینترفرون گاما در موش‌های تحت تنش گرمایی شد. بیان نسبی ژن اینترلوکین2 در گروه‌های دریافت کننده مکمل سلنیوم نسبت به گروه شاهد مثبت پایین‌تر بود. همچنین افزودن مکمل سلنیوم به جیره سبب کاهش بیان ژن اینترلوکین6 در مقایسه با تیمار شاهد مثبت گردید، به طوری که پایین‌ترین بیان ژن اینترلوکین6 در موش‌های تحت تنش گرمایی مربوط به تیمار0/45 میلی‌گرم بود. نتایج حاکی از افزایش غلظت ایمونوگلوبولین G با افزودن مکمل سلنیوم به جیره بود.
المصادر:
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3. Ringuet MT, Hunne B, Lenz M, Bravo DM, Furness JB. Analysis of Bioavailability and Induction of Glutathione Peroxidase by Dietary Nanoelemental, Organic and Inorganic Selenium. Nutrients. 2021 Mar 25;13(4):1073. Doi: 10.3390/nu13041073.
4. Jones GD, Droz B, Greve P, Gottschalk P, Poffet D, McGrath SP, Seneviratne SI, Smith P, Winkel LH. Selenium deficiency risk predicted to increase under future climate change. Proceedings of the National Academy of Sciences. 2017 Mar 14;114(11):2848-53. Doi: 10.1073/pnas.1611576114.
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19. Goldsby RA, Kindt TJ, Osborne B, Kuby J (2000) Kuby immunology, 4th edn. WH Freeman and Company, New York, pp 303–327.
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23. Xu D, Li W, Huang Y, He J, Tian Y. The effect of selenium and polysaccharide of Atractylodes macrocephala Koidz.(PAMK) on immune response in chicken spleen under heat stress. Biological Trace Element Research. 2014 Aug;160:232-7. Doi: 10.1007/s12011-014-0056-y. Epub 2014 Jun 26.
24. Luan Y, Zhao J, Yao H, Zhao X, Fan R, Zhao W, Zhang Z, Xu S. Selenium deficiency influences the mRNA expression of selenoproteins and cytokines in chicken erythrocytes. Biological trace element research. 2016 Jun;171:427-36. Doi: 10.1007/s12011-015-0536-8.
25. Khoso PA, Yang Z, Liu C, Li S. Selenoproteins and heat shock proteins play important roles in immunosuppression in the bursa of Fabricius of chickens with selenium deficiency. Cell Stress and Chaperones. 2015 Nov 1;20(6):967-78. Doi: 10.1007/s12192-015-0625-9.
26. Tsuji PA, Carlson BA, Anderson CB, Seifried HE, Hatfield DL, Howard MT. Dietary selenium levels affect selenoprotein expression and support the interferon-γ and IL-6 immune response pathways in mice. Nutrients. 2015 Aug 6;7(8):6529-49. Doi: 10.3390/nu7085297.
27. Ren F, Chen X, Hesketh J, Gan F, Huang K. Selenium promotes T-cell response to TCR-stimulation and ConA, but not PHA in primary porcine splenocytes. PLoS One. 2012 Apr 17;7(4):e35375. doi: 10.1371/journal.pone.0035375.
28. Meissonnier GM, Pinton P, Laffitte J, Cossalter AM, Gong YY, Wild CP, Bertin G, Galtier P, Oswald IP. Immunotoxicity of aflatoxin B1: impairment of the cell-mediated response to vaccine antigen and modulation of cytokine expression. Toxicology and applied pharmacology. 2008 Sep 1;231(2):142-9. Doi: 10.1016/j.taap.2008.04.004.
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32. Zheng Y, Zhao Y, He W, Wang Y, Cao Z, Yang H, Wang W, Li S. Novel organic selenium source hydroxy-selenomethionine counteracts the blood-milk barrier disruption and inflammatory response of mice under heat stress. Frontiers in Immunology. 2022 Dec 1;13:1054128. Doi: 10.3389/fimmu.2022.1054128.
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1. Pecoraro BM, Leal DF, Frias-De-Diego A, Browning M, Odle J, Crisci E. The health benefits of selenium in food animals: a review. Journal of animal science and biotechnology. 2022 May 13;13(1):58. Doi: 10.1186/s40104-022-00706-2.
2. Surai PF. Organic selenium vs. its combination with sodium selenite in poultry nutrition: food for thoughts. Poult Sci. 2021 Oct;100(10):101311. Doi: 10.1016/j.psj.2021.101311.
3. Ringuet MT, Hunne B, Lenz M, Bravo DM, Furness JB. Analysis of Bioavailability and Induction of Glutathione Peroxidase by Dietary Nanoelemental, Organic and Inorganic Selenium. Nutrients. 2021 Mar 25;13(4):1073. Doi: 10.3390/nu13041073.
4. Jones GD, Droz B, Greve P, Gottschalk P, Poffet D, McGrath SP, Seneviratne SI, Smith P, Winkel LH. Selenium deficiency risk predicted to increase under future climate change. Proceedings of the National Academy of Sciences. 2017 Mar 14;114(11):2848-53. Doi: 10.1073/pnas.1611576114.
5. Milewski S, Sobiech P, Błażejak-Grabowska J, Wójcik R, Żarczyńska K, Miciński J, Ząbek K. The efficacy of a long-acting injectable selenium preparation administered to pregnant ewes and lambs. Animals. 2021 Apr 9;11(4):1076. Doi: 10.3390/ani11041076. PMID: 33918972; PMCID: PMC8070106.
6. Zoidis E, Seremelis I, Kontopoulos N, Danezis GP. Selenium-dependent antioxidant enzymes: Actions and properties of selenoproteins. Antioxidants. 2018 May 14;7(5):66. Doi: 10.3390/antiox7050066.
7. Sun LL, Gao ST, Wang K, Xu JC, Sanz-Fernández MV, Baumgard LH, Bu DP. Effects of source on bioavailability of selenium, antioxidant status, and performance in lactating dairy cows during oxidative stress-inducing conditions. Journal of dairy science. 2019 Jan 1;102(1):311-9. Doi: 10.3168/jds.2018-14974.
8. Belhadj Slimen I, Najar T, Ghram A, Abdrrabba MJ. Heat stress effects on livestock: molecular, cellular and metabolic aspects, a review. Journal of animal physiology and animal nutrition. 2016 Jun;100(3):401-12. Doi: 10.1111/jpn.12379.
9. Ren Z, Okyere SK, Zhang M, Zhang X, He H, Hu Y. Glycine nano-selenium enhances immunoglobulin and cytokine production in mice immunized with H9N2 avian influenza virus vaccine. International Journal of Molecular Sciences. 2022 Jul 18;23(14):7914. Doi: 10.3390/ijms23147914.
10. Shi L, Ren Y, Zhang C, Yue W, Lei F. Effects of organic selenium (Se-enriched yeast) supplementation in gestation diet on antioxidant status, hormone profile and haemato-biochemical parameters in Taihang Black Goats. Animal Feed Science and Technology. 2018 Apr 1;238:57-65. DOI:10.1016/j.anifeedsci.2018.02.004.
11. Bogye G, Alfthan G, Machay T. Randomized clinical trial of enteral yeast‐selenium supplementation in preterm infants. Biofactors. 1998;8(1‐2):139-42. Doi: 10.1002/biof.5520080123.
12. Novoselec J, Klir Šalavardić Ž, Đidara M, Novoselec M, Vuković R, Ćavar S, Antunović Z. The effect of maternal dietary selenium supplementation on blood antioxidant and metabolic status of ewes and their lambs. Antioxidants. 2022 Aug 26;11(9):1664. doi: 10.3390/antiox11091664.
13. Beheshtipour H, Mortazavian AM, Mohammadi R, Sohrabvandi S, Khosravi‐Darani K. Supplementation of Spirulina platensis and Chlorella vulgaris algae into probiotic fermented milks. Comprehensive Reviews in Food Science and Food Safety. 2013 Mar;12(2):144-54. Doi.org/10.1111/1541-4337.12004.
14. Liu K, Ding T, Fang L, Cui L, Li J, Meng X, Zhu G, Qian C, Wang H, Li J. Organic selenium ameliorates Staphylococcus aureus-induced mastitis in rats by inhibiting the activation of NF-κB and MAPK signaling pathways. Frontiers in Veterinary Science. 2020 Jul 30;7:443. Doi: 10.3389/fvets.2020.00443.
15. Mehdi Y, Dufrasne I. Selenium in cattle: a review. Molecules. 2016 Apr 23;21(4):545. Doi: 10.3390/molecules21040545.
16. Rawash RA, Sharaby MA, Hassan GE, Elkomy AE, Hafez EE, Hafsa SH, Salem MM. Expression profiling of HSP 70 and interleukins 2, 6 and 12 genes of Barki sheep during summer and winter seasons in two different locations. International journal of biometeorology. 2022 Oct;66(10):2047-53. DOI: 10.1007/s00484-022-02339-6.
17. Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2− ΔΔCT method. methods. 2001 Dec 1;25(4):402-8. Doi: 10.1006/meth.2001.1262.
18. Voller A, Bidwell DE, Bartlett A, Fleck DG, Perkins M, Oladehin B. A microplate enzyme-immunoassay for toxoplasma antibody. Journal of clinical pathology. 1976 Feb 1;29(2):150-3. Doi: 10.1136/jcp.29.2.150.
19. Goldsby RA, Kindt TJ, Osborne B, Kuby J (2000) Kuby immunology, 4th edn. WH Freeman and Company, New York, pp 303–327.
20. Liao X, Lu L, Li S, Liu S, Zhang L, Wang G, Li A, Luo X. Effects of selenium source and level on growth performance, tissue selenium concentrations, antioxidation, and immune functions of heat-stressed broilers. Biological Trace Element Research. 2012 Dec;150:158-65. Doi: 10.1007/s12011-012-9517-3.
21. Xu T, Deng R, Li X, Zhang Y, Gao MQ. RNA-seq analysis of different inflammatory reactions induced by lipopolysaccharide and lipoteichoic acid in bovine mammary epithelial cells. Microbial pathogenesis. 2019 May 1;130:169-77. Doi: 10.1016/j.micpath.2019.03.015.
22. Chen K, Yuan S, Chen J, Peng X, Wang F, Cui H, Fang J. Effects of sodium selenite on the decreased percentage of T cell subsets, contents of serum IL-2 and IFN-γ induced by aflatoxin B1 in broilers. Research in veterinary science. 2013 Aug 1;95(1):143-5. Doi: 10.1016/j.rvsc.2013.02.019.
23. Xu D, Li W, Huang Y, He J, Tian Y. The effect of selenium and polysaccharide of Atractylodes macrocephala Koidz.(PAMK) on immune response in chicken spleen under heat stress. Biological Trace Element Research. 2014 Aug;160:232-7. Doi: 10.1007/s12011-014-0056-y. Epub 2014 Jun 26.
24. Luan Y, Zhao J, Yao H, Zhao X, Fan R, Zhao W, Zhang Z, Xu S. Selenium deficiency influences the mRNA expression of selenoproteins and cytokines in chicken erythrocytes. Biological trace element research. 2016 Jun;171:427-36. Doi: 10.1007/s12011-015-0536-8.
25. Khoso PA, Yang Z, Liu C, Li S. Selenoproteins and heat shock proteins play important roles in immunosuppression in the bursa of Fabricius of chickens with selenium deficiency. Cell Stress and Chaperones. 2015 Nov 1;20(6):967-78. Doi: 10.1007/s12192-015-0625-9.
26. Tsuji PA, Carlson BA, Anderson CB, Seifried HE, Hatfield DL, Howard MT. Dietary selenium levels affect selenoprotein expression and support the interferon-γ and IL-6 immune response pathways in mice. Nutrients. 2015 Aug 6;7(8):6529-49. Doi: 10.3390/nu7085297.
27. Ren F, Chen X, Hesketh J, Gan F, Huang K. Selenium promotes T-cell response to TCR-stimulation and ConA, but not PHA in primary porcine splenocytes. PLoS One. 2012 Apr 17;7(4):e35375. doi: 10.1371/journal.pone.0035375.
28. Meissonnier GM, Pinton P, Laffitte J, Cossalter AM, Gong YY, Wild CP, Bertin G, Galtier P, Oswald IP. Immunotoxicity of aflatoxin B1: impairment of the cell-mediated response to vaccine antigen and modulation of cytokine expression. Toxicology and applied pharmacology. 2008 Sep 1;231(2):142-9. Doi: 10.1016/j.taap.2008.04.004.
29. Zinchuk A, Holubovska O, Shkurba A, Hrytsko R, Vorozhbyt O, Richniak M, Herasun B. Original inhibition method of excessive synthesis of pro-inflammatory cytokine of tumour necrosis factor α. Central European Journal of Immunology. 2015 Oct 15;40(3):345-8. Doi: 10.5114/ceji.2015.54597.
30. Zhang Y, Xu Y, Chen B, Zhao B, Gao XJ. Selenium deficiency promotes oxidative stress-induced mastitis via activating the NF-κB and MAPK pathways in dairy cow. Biological Trace Element Research. 2022 Jun;200(6):2716-26. Doi: 10.1007/s12011-021-02882-0.
31. Liu G, Qi M, Hutchinson MR, Yang G, Goldys EM. Recent advances in cytokine detection by immunosensing. Biosensors and Bioelectronics. 2016 May 15;79:810-21. Doi: 10.1016/j.bios.2016.01.020.
32. Zheng Y, Zhao Y, He W, Wang Y, Cao Z, Yang H, Wang W, Li S. Novel organic selenium source hydroxy-selenomethionine counteracts the blood-milk barrier disruption and inflammatory response of mice under heat stress. Frontiers in Immunology. 2022 Dec 1;13:1054128. Doi: 10.3389/fimmu.2022.1054128.
33. Wang H, Bi C, Wang Y, Sun J, Meng X, Li J. Selenium ameliorates Staphylococcus aureus-induced inflammation in bovine mammary epithelial cells by inhibiting activation of TLR2, NF-κB and MAPK signaling pathways. BMC veterinary research. 2018 Dec;14:1-8. Doi: 10.1186/s12917-018-1508-y.
34. Abdel-Moneim AM, Shehata AM, Mohamed NG, Elbaz AM, Ibrahim NS. Synergistic effect of Spirulina platensis and selenium nanoparticles on growth performance, serum metabolites, immune responses, and antioxidant capacity of heat-stressed broiler chickens. Biological Trace Element Research. 2022 Feb 1:1-2. Doi: 10.1007/s12011-021-02662-w.
35. Tang J, Cao L, Jia G, Liu G, Chen X, Tian G, Cai J, Shang H, Zhao H. The protective effect of selenium from heat stress-induced porcine small intestinal epithelial cell line (IPEC-J2) injury is associated with regulation expression of selenoproteins. British journal of nutrition. 2019 Nov;122(10):1081-90. Doi: 10.1017/S0007114519001910.
36. Khan AZ, Khan IU, Khan S, Afzal S, Hamid M, Tariq M, Haq IU, Ullah N, Khan MA, Bilal S, Huwang K. Selenium-enriched probiotics improve hepatic protection by regulating pro-inflammatory cytokines and antioxidant capacity in broilers under heat stress conditions. Journal of advanced veterinary and animal research. 2019 Sep;6(3):355. Doi: 10.5455/javar.2019.f354.
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