تاثیر هشت هفته تمرین مقاومتی در ترکیب با مکمل BCAA نانولیپوزوم بر بیان ژن های HSP60 و HSP70 میتوکندری کاردیومیوسیت های رتهای مدل سکته قلبی
الموضوعات :
الهام فرهادفر
1
,
ماکان خواجه وندی
2
1 - گروه تربیت بدنی و علوم ورزشی، دانشگاه آزاد اسلامی، واحد دزفول، دزفول
2 - گروه تربیت بدنی و علوم ورزشی، واحد تهران مرکزی، دانشگاه آزاد اسلامی، تهران، ایران
الکلمات المفتاحية: تمرین مقاومتی, مکمل BCAA نانولیپوزوم, HSP60 و HSP70 و رتهای مدل سکته قلبی.,
ملخص المقالة :
هدف از پژوهش حاضر بررسی تاثیر هشت هفته تمرین مقاومتی در ترکیب با مکمل BCAA نانولیپوزوم بر بیان ژنهای HSP60 و HSP70 میتوکندری کاردیومیوسیتهای رتهای مدل سکته قلبی بود. در اين مطالعه تجربي، 32 سر موش صحرایی نر نژاد ویستار مدل سکته قلبی به صورت تصادفی به 4 گروه کنترل، مکمل (BCAA نانولیپوزوم)، توام (تمرین مقاومتی+ مکمل BCAAنانولیپوزوم) و تمرین (تمرین مقاومتی) تقسیم شدند. تمرين مقاومتي شامل هشت هفته تمرين نردبان با شدت متوسط (70 درصد از MVCC) و پنج روز در هفته بود. در گروه¬های مکمل و توام 5 روز در هفته و به مدت 8 هفته، مکمل BCAA نانولیپوزوم و به میزان 600 میلی گرم به ازای هر کیلوگرم وزن بدن به صورت گاواژ دریافت شد. بیان نسبی ژنهای HSP60 و HSP70 میتوکندری با استفاده از روش Real-time PCR به دست آمد. ﺩﺍﺩﻩ ﻫﺎ ﺑﺎ ﺍﺳﺘﻔﺎﺩ ﺍﺯ ﺁﺯﻣﻮن ﺁﻣﺎری تحلیل واریانس دوطرفه ﻭ ﺁﺯﻣﻮﻥ تعقیبی توکیﺁﻧﺎﻟﻴﺰ ﺷﺪﻧﺪ. كليه عمليات آماري توسط نرم افزار(SPSS) نسخه 23 و سطح معني داري آزمون ها در سطح 05/0≥p مورد تجزیه و تحلیل قرار گرفتند.نتایج آزمون نشان داد که ميانگين بیان ژنهای HSP60 و HSP70 کاردیومیوسیت‌ها در گروه‌های تمرين کرده با و بدون مصرف BCAA (تمرین+ مکمل و تمرین) به طور معنی‌داری بیشتر از گروه‌های تمرين نکرده با و بدون مصرف BCAA (مکمل و کنترل) است. نتایج تحقيق اهميت HSP60 و HSP70 را در تكثير و تمایز سلول های عضلانی همراه با بازسازی از آسيب ناشی از انفارکتوس قلبی را نشان می دهد.
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