تکثیر درون‌شیشه‌ای موثر ارکید فالانوپسیس رقم Karen Rockwell
الموضوعات : مجله گیاهان زینتی
ابوالفضل ولی زاده
1
(گروه فضای سبز، واحد نور، دانشگاه آزاد اسلامی، نور، ایران)
جلال محمودی
2
(گروه فضای سبز، واحد نور، دانشگاه آزاد اسلامی، نور، ایران)
محسن محمدی
3
(گروه باغبانی، واحد رشت، دانشگاه آزاد اسلامی، رشت، ایران)
بهزاد کاویانی
4
(گروه باغبانی، واحد رشت، دانشگاه آزاد اسلامی، رشت، ایران)
الکلمات المفتاحية: زغال فعال, ارکیداسه, تنظیم‌کننده‌های رشد گیاهی, اجسام شبه‌پروتوکورم, کشت بافت,
ملخص المقالة :
فالانوپسیس یک جنس ارکید با ارزش اقتصادی بالا در گلکاری جهان است که به عنوان یک گیاه گلدانی و گل شاخه‌بریده استفاده می‌شود. تنوع ژنتیکی بالا و عدم یکنواختی رویشی و تکثیر تولیدمثلی، تولید این ارکید را از نظر اقتصادی غیر جالب می‌کند. تکثیر درون‌شیشه‌ای تنها روش امکان‌پذیر در مقیاس بزرگ برای تکثیر فالانوپسیس است. هدف از مطالعه حاضر، بررسی اثر انواع و غلظت‌های -ɑنفتالن استیک اسید (NAA) و 6-بنزیل‌آدنین (BA) (هر دو در غلظت‌های ۰، 0/5، ۱، 1/5 و ۳ میلی‌گرم در لیتر، به‌صورت جداگانه یا ترکیبی)، در قالب طرح کاملا تصادفی در تکثیر درون‌شیشه‌ای فالانوپسیس بود. زغال فعال (AC) (۰، 0/5 و ۱ میلی‌گرم در لیتر) برای جلوگیری از قهوه‌ای‌شدن محیط و بافت‌ها به محیط اضافه شد. موراشیگ و اسکوگ (MS)و پروتوکورم به‌ترتیب به‌عنوان محیط کشت و ریزنمونه استفاده شدند. نتایج نشان داد که بیشترین تعداد برگ در محیط غنی‌شده با ۱ میلی‌گرم در لیتر NAA همراه با 5/1 میلی‌گرم در لیتر BA و ۱ میلی‌گرم در لیتر AC به‌دست آمد. تیمار حاوی ۵/۱ میلی‌گرم در لیتر NAA همراه با 0/5 میلی‌گرم در لیتر BA و ۱ میلی‌گرم در لیتر AC بیشترین تعداد ریشه را القا کرد. گیاهچه‌های کامل تولیدشده در شرایط درون‌شیشه‌ای به گلدان‌های حاوی مخلوطی از LECA (دانه‌های خاک رس منبسط‌شده سبک)، پیت ماس، کوکوپیت، خاک زغال چوب، کوکو چیپس و پرلیت منتقل شدند و در شرایط گلخانه‌ای با درصد بقای ۱۰۰ درصد سازگار شدند.
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