اثر اولئوروپین بر میزان گلوکز خون و بیان ژن پیروات کیناز در موش های صحرایی نر دیابتی شده با استرپتوزوتوسین

اثر اولئوروپین بر  گلوکز خون و ژن پیروات کیناز در موش های صحرایی دیابتی 

الموضوعات :

اکرم اسدی ¹ , مریم رفیعی راد ² , آمنه جاوید ³

1 - علم و هنر یزد
2 - گروه زیست شناسی دانشگاه آزاد اسلامی واحد ایذه
3 - گروه زیست شناسی دانشکده مهندسی و علوم دانشگاه علم و هنر یزد

تاريخ الإرسال : 20 الجمعة , شعبان, 1445 تاريخ التأكيد : 29 الأربعاء , شوال, 1445 تاريخ الإصدار : 14 الأربعاء , ذو القعدة, 1445

الکلمات المفتاحية: دیابت, اولئوروپین, پیروات کیناز, موش صحرایی,

ملخص المقالة :

زمینه و هدف: با توجه به خواص آنتی‎ ‎اکسیدانی‎ ‎اولئوروپین و نقش آنتی اکسیدان‎ ‎ها در بهبود دیابت، این مطالعه با هـدف ‏بررسی اثر اولئوروپین بر میزان گلوکز خون و بیان ژن پیروات کیناز در موش های صحرایی نر دیابتی انجام شد.‏ مواد و روش ها : با تزریق استرپتوزوتوسین (‏mg/kg‏60) در موش هاي صحرایی نر (250-200گرم) دیابت ایجـاد شـد. ح پس ‏از تزریق داخل صفاقی ‏STZ، حیوانات دارای قند خون بالای 200‏mg/dL، دیابتی تلقی شدند. گروه‌های کنترل و گروه دیابتی ‏فقط سالین و گروه‌های دیابتی درمان شده به مدت چهار هفته روزانه بصورت گاواژ اولئوروپین‎ mg/kg‏ 10،20دریافت کردند. ‏سپس رت ها با دارو بی ‌هوش و تشریح شدند. بافت کبد جداسازی و برای بررسی بیان ژن پیروات کیناز به روش ‏Real-time ‎PCR‏ استفاده شد. تجزیه و تحلیل داده‌ها با استفاده از آزمون توکی در سطح ‏P<0.05‎‏ انجام شد. ‏ نتایج : نتایج نشان داد در گروه دیابتی دریافت کننده دوزهای ‏mg/kg‏ 20و10 اولئوروپین نسبت به گروه دیابتی به طور ‏معناداری کاهش یافت. بررسی بیان ژن پیروات کیناز نیز نـشان داد در گروه دیابتی ﻧﺴﺒﺖ ﺑﻪ گروه کنترل ﺳﺎﻟﻢ ﺩﺍﺭﺍﻱ کاهش ﺑﻮﺩ ‏ﺍﻣﺎ ﺍﻳﻦ کاهش ﺍﺯ ﻧﻈﺮ ﺁﻣﺎﺭﻱ ﺩﺭ ﺣﺪ ﻣﻌﻨﻲﺩﺍﺭﻱ ﺑﻴﺎﻥ ﻧﺸﺪ. از طرفی ﺩﺭ گروه دیابتی تحت درمان با دوز های ‏mg/kg‏ 20 و 10 ‏اولئوروپین میزان بیان ژن پیروات کیناز نسبت به گروه دیابتی به طور معناداری افزایش داشته است.‏ نتیجه گیری: نتایج تحقیق حاضر نشان می دهد که احتمالا تجویز اولئوروپین توانسته از طریق تغییر میزان بیان ژن پیرووات ‏کیناز باعث کاهش گلوکز خون شود‏

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