بررسی اثرات سایتوتوکسیک ناندرولون بر رده سلولی سرطان کولون (HCT) و ارزیابی فعالیت کاسپازهای 3، 8 و 99
الموضوعات : فصلنامه زیست شناسی جانوری
زهرا اعلایی
1
,
عبدالحسین شیروی
2
,
رحیم احمدی
3
,
ویدا حجتی
4
1 - گروه زیست شناسی، واحد دامغان، دانشگاه آزاد اسلامی، دامغان، ایران
2 - گروه زیست شناسی، واحد دامغان، دانشگاه آزاد اسلامی، دامغان، ایران
3 - گروه فیزیولوژی، واحد همدان، دانشگاه آزاد اسلامی، همدان، ایران
4 - گروه زیست شناسی، واحد دامغان، دانشگاه آزاد اسلامی، دامغان، ایران
الکلمات المفتاحية: زنده‌مانی, Hct, کاسپاز 3, کاسپاز 9, ناندرولون, کاسپاز 8,
ملخص المقالة :
سرطان کولون از مهمترین سرطان ها در سراسر دنیا محسوب می شود و سومین سرطان شایع در مردان و دومین سرطان شایع در زنان می باشد. مطالعات نشان داده اند که هورمون های استروئیدی جنسی بر عملکرد دستگاه گوارش و بر تکثیر سلول های سرطانی در سطح سلولی و مولکولی تاثیرگذارند. این مطالعه به بررسی اثرات ضد تکثیری ناندرولون بر رده سلولی سرطان کلون (HCT) و ارزیابی فعالیت کاسپازهای 3، 8 و 9 می پردازد. در این مطالعه آزمایشگاهی سلول های HCT به گروه شاهد (بدون تیمار) و گروه های تیمار شده با غلظت های625/0، 25/1، 5/2، 5 و 10 میلی گرم بر میلی‌لیتر ناندرولون تقسیم بندی شدند. اثر سیتوتوکسیک هورمون با استفاده از سنجش MTT اندازه گیری شد. میزان فعالیت کاسپازهای 3، 8 و 9 توسط کیت (Novex) مورد ارزیابی قرار گرفت. زنده مانی سلول های HCT در گروه دریافت کننده ی غلظت های 5/2، 5 و 10 میلی‌گرم بر میلی‌لیتر ناندرولون نسبت به گروه شاهد دچار کاهش معناداری شد (001/0p <) در حالی که فعالیت کاسپاز 3 و 9 (001/0p <) و 8 (01/0 < p) در رده سلولی HCT تیمار شده با ناندرولون، افزایش معناداری پیدا کرد. یافته های این تحقیق نشان دادند که ناندرولون دارای اثر سایتوتوکسیک بر روی سلول های سرطانی کولون می باشد و بخشی از این اثر حداقل از طریق افزایش فعالیت کاسپازهای 3 و 8 و 9 حاصل می شود.
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