The Effects of Eight Weeks of Submaximal Running and High-fat Diet on IL-6 Gene Expression in the Muscle Tissue of Male Rats
الموضوعات :
Shima Ebadi
1
,
Ardeshir Zafari
2
1 - Department of Sports Sciences, Faculty of Humanities and Art, Zanjan Branch, Islamic Azad University, Zanjan, Iran
2 - Department of Sports Sciences, Faculty of Humanities and Art, Zanjan Branch, Islamic Azad University, Zanjan, Iran
الکلمات المفتاحية: Aerobic training, body weight, High-fat diet, Inter Leukin-6,
ملخص المقالة :
It seems that the release of IL-6 from the contracting muscles and accumulation in the blood circulation has a close relationship with the duration and intensity of physical activity. The aime of this experimental study was to determine the effects of eight weeks of submaximal running and high-fat diet on IL-6 gene expression levels in the muscle tissue of an animal model. At the end of the two-week period of keeping under controlled conditions, 40 two-month-old male rats were randomly divided into four equal groups of control, high-fat diet, submaximal running, and a combination of high-fat diet and submaximal running. The training groups participated in the aerobic running protocol on the treadmill for five days a week for eight weeks. The polymerase chain reaction method was used to determine the levels of IL-6 gene expression in muscle tissue. One-way analysis of variance was used to determine between-group differences. The effects of high-fat diet and running with submaximal intensity on IL-6 gene expression in muscle tissue are significant. The consumption of high-fat food causes adverse changes in the expression of IL-6 gene, but eight weeks of running with sub-maximal intensity moderates the adverse effects of high-fat diet and creates favorable changes in the expression of IL-6 gene and body weight.
Church TS, Thomas DM, Tudor-Locke C, Katzmarzyk PT, Earnes, CP, Rodarte RQ, (2011) Trends over 5 decades in US occupation-related physical activity and their association with obesity. PloS one. 6(5), e19657.
Da Cunha Nascimento D, de Sousa NMF, de Sousa Neto IV, Tibana RA, de Souza VC, Vieira DCL (2015) Classification of pro-inflammatory status for interleukin-6 affects relative muscle strength in obese elderly women. Aging Clinical and Experimental Research. 27(6), 791-797.
Ebrahimi M, Khenar Sanami S (2015) Effects of high fat diet and high intensity aerobic training on interleukin 6 plasma levels in rats. Modares Journal of Medical Sciences: Pathobiology. 18(3), 110-116.
Eftekhari E, Zafari A, Gholami M (2016) Physical activity, lipid profiles and leptin. The Journal of Sports Medicine and Physical Fitness. 56(4), 465-469.
Ellomi M, Ben Ounis O, Makni E, Van Praagh E, Tabka Z, Lac G (2009) Effect of individualized weight-loss programmes on adiponectin, leptin and resistin levels in obese adolescent boys. Acta Pediatr. 98(9), 1487-1493.
Ezadi M, Sohaily S, Parsian H, Zand A (2011) Pro-inflammatory cytokine Interleukin-1 beta is associated with cardiovascular fitness in sedentary diabetic patients. Journal of Biodiversity and Environmental Sciences (JBES). 1(2), 37-44.
Loos RJ (2012) Genetic determinant of common obesity and their value in prediction. Best Practice & Research Clinical Endocrinology & Metabolism. 26(2), 211-226.
Na YK, Hong HS, Lee WK, Kim DS (2015) Increased methylation of interleukin 6 gene is associated with obesity in Korean women. Molecular and Cells. 38(5), 452-456.
Nemeth E, Rivera S, Gabayan V, Keller C, Taudorf S, Pederson BK (2004) IL-6 mediates hypoferremia of inflammation by inducing the synthesis of the iron regulatory hormone hepcidin. The Journal of Clinical Investigation. 113(9), 1271-1276.
Noura M, Arshadi S, Zafari A, Banaeifar A (2020) The effect of running on positive and negative slopes on TNF-α and INF- γ gene expression in the muscle tissue of rats with Alzheimer’s disease. Journal of Basic Research in Medical Science. 7(1), 35-42.
Ossanloo P, Najar L, Zafari A (2012) The Effects of Combined Training (Aerobic Dance, Step Exercise and Resistance Training) on Body Fat Percent and Lipid Profiles in Sedentary Females of AL_ZAHRA University. European Journal of Experimental Biology. 2(5), 1598-1602.
Ouchi N, Parker JL, Lugus JJ, Walsh K (2011) Adipokines in inflammation and metabolic disease. Nature Reviews Immunology. 11(2), 85-97.
Sindhu S, Thomas R, Shihab P, Sriraman D, Behbahani K, Ahmad R (2015) Obesity is a positive modulator of IL-6R and IL-6 expression in the subcutaneous adipose tissue: significance for metabolic inflammation. PloS one. 10(7), e0133494.
Sokhanvardastjerdi S, Banaeifar A, Arshadi S, Zafari A (2020) the Effect of 12 Weeks Aerobic Training on PDX-1 and GLUT2 Gene Expression in the Pancreatic Tissue of Type 2 Diabetic Rats. Iranian journal of Diabetes and Obesity. 12(2), 98-103.
Tanaka T, Narazaki M, Kishimoto T (2014) IL-6 in inflammation, immunity, and disease. Cold Spring Harbor Perspective in Biology. 6(10), a016295.
Vieira VJ, Valentine RJ, Wilund KR, Antao N, Baynard T, Woods JA (2009) Effects of exercise and low-fat diet on adipose tissue inflammation and metabolic complications in obese mice. American Journal of Physiology – Endocrinology and Metabolism. 296(5), 1164-71.
