• الصفحة الرئيسية
  • Effect of Purified Bacteriocin from Acinetobacter baumannii on some Pathogenic and Environmental Isolates and Its Inhibitory Effect on Hemolysin Production from S. aureu

شارک

عنوان URL للمقالة


رقم المقالة : 691877 زيارة : 207 الصفحة: 649 - 658

10.22034/jchr.2022.691877

نوع المخطوط: ابحاث

Effect of Purified Bacteriocin from Acinetobacter baumannii on some Pathogenic and Environmental Isolates and Its Inhibitory Effect on Hemolysin Production from S. aureu

الموضوعات :

Sahar Jabar Nasser 1 (Department of Biology, College of Science, Al-Mustansiriyah University, Baghdad, Iraq)
Raghad A. Abdulrazaq 2 (Department of Biology, College of Science, Al-Mustansiriyah University, Baghdad, Iraq)

تاريخ الإرسال : 25 الأحد , جمادى الثانية, 1442 تاريخ التأكيد : 24 السبت , شوال, 1442 تاريخ الإصدار : 07 الخميس , جمادى الأولى, 1444

الکلمات المفتاحية: S. aureus, Acinetobacter baumannii, Purified bacteriocin, Hemolysin production,

ملخص المقالة :

From 122 isolates 63 isolates were diagnosed as Acinetobacter baumannii. The highest percentage of isolation was 37 (58.7%) from wounds and the lower percentage was 7 (11.1%) from burns. The antimicrobial susceptibility test was performed the highest resistance percentage was Cephalothin at 95.2 %, while the low percent was gentamycin was 47.6 %. The minimal inhibitory concentrations (MICs) for imipenem were tested the MIC values for imipenem resistant isolates ranged from 16 µg ml-1 to 64 µg ml-1. Bacteriocins proteinaceous or peptidic toxins produced by bacteria offer promising potential as substitutes or conjugates to current therapeutic compounds. Three methods were used to detect bacteriocin production Acinetobacter baumannii, Agar well diffusion method, cup assay, and Disk method. From 63 Acinetobacter baumannii isolates 27 (42.85%) isolates were able to produce bacteriocin. Agar well diffusion method was the best for detection and the best isolate was (No. 48) for bacteriocin production for all methods. Bacteriocin purified by two steps method ammonium sulfate precipitation followed by gel filtration chromatography on Sephadex G150 column and characterized. The specific activity was 1541U mg protein with 6.3 bacteriocin folds and 64% recovery yield. The molecular weight of bacteriocin was (14000) Da. Bacteriocin activity was stable at pH values (3-9). Also, bacteriocin showed high thermostability at different temperatures (20-80)°C for (30) min. The antimicrobial activity of crude and purified Acinetobacter baumannii bacteriocin from (No. 48) isolate was maximum antibacterial activity against E. coli , S. aureus (purified 30 mm, crude 22 mm for both) and K. pneumonia isolates (purified 25 mm, crude 20 mm).

المصادر:


  1. Zheng Z., Zhang D., Li W., Qin W., Huang X., Lv L., 2018. Substrates removal and growth kinetic characteristics of a heterotrophic nitrifying-aerobic denitrifying bacterium, Acinetobacter harbinensis HITLi7T at 2°C. Bioresource Technology. 259, 286-293.
    2.
  2. Pepper I.L., Gerba C.P., Gentry T.J., 2015. Introduction to Environmental Microbiology. In Environmental Microbiology. Academic Press. 519. pp. 3-8.
    3.
  3. Dewachi O., 2019. Iraqibacterand the Pathologies of Intervention. Middle East Report 290.
    4.
  4. Harding C.M., Hennon S.W., Feldman M.F., 2018. Uncovering the mechanisms of Acinetobacter baumannii virulence. Nature Reviews Microbiology. 16(2), 91-102.
    5.
  5. Luo L.M., Wu L.J., Xiao Y.L., Zhao D., Chen Z.X., Kang M., Zhang Q., Xie Y., 2015. Enhancing pili assembly and biofilm formation in Acinetobacter baumannii ATCC19606 using non-native acyl-homoserine lactones. BMC Microbiology. 15(1), 1-7.
    6.
  6. Wong D., Nielsen T.B., Bonomo R.A., Pantapalangkoor P., Luna B., Spellberg B., 2017. Clinical and Pathophysiological Overview of Acinetobacter Infections: a Century of Challenges. Clinical Microbiology Reviews. 30(1), 409–447.
    7.
  7. Wrenn C., 2015. Introduction of the VITEK 2 Compact and Implementation of EUCAST Guidelines in a Microbiology Department. [Masters dissertation]. Dublin: Royal College of Surgeons in Ireland; 2015.
    8.
  8. Clinical and Laboratory Standards Institute (CLSI - formerly NCCLS). 2017. Performance Standards for Antimicrobial Disk Susceptibility Tests; Approved Standard – Twelfth Edition. M02-A26. CLSI, Wayne, PA.
    9.
  9. Lewus C.B, Kaiser A., Montville T.J., 1991. Inhibition of food-borne bacterial pathogens by bacteriocins from lactic acid bacteria isolated from meat. Appl Environ Microbiol. 57(6), 1683-1688.
    10.
  10. Al-Qassab A.O., Al-Khafaji Z.M., 1992. Effect of different conditions on inhibition activity of enteric lactobacilli against diarrhea-causing enteric bacteria. Journal of Agricultural Science. 3(1), 18-26.
    11.
  11. Tagg J.R., McGiven A.R., 1971. Assay system for bacteriocins. Applied Microbiology. 21, 943.
    12.
  12. Parente E., Brienza C., Moles M., Ricciardi A., 1995. A comparison of methods for the measurement of bacteriocin activity. Journal of Microbiological Methods. 22(1), 95-108.
    13.
  13. Pilasombut K., Sakpuaram T., Wajjwalku W., Nitisinprasert S., Swetwiwathana A., Zendo T., Fujita K., Nakayama J., Sonomoto K., 2006. Purification and amino acid sequence of a bacteriocins produced by Lactobacillus salivarius K7 isolated from chicken intestine. Songklanakarin J Sci Technol. 28(Suppl 1), 121-131.
    14.
  14. Chhibber S., Vadehra D.V., 1986. Purification and characterization of a bacteriocin from Klebsiella pneumoniae 158. J Gen Microbiol. 132(4), 1051-4.
    15.
  15. KP S., PV K., PK B., RC R., PB D., AK S., 2016. Production and characterization of bacteriocin produced by Lactobacillus viridescence (NICM 2167). Brazilian Archives of Biology and Technology. 59, e16150518.
    16.
  16. Classics Lowry O., Rosebrough N., Farr A., Randall R., 1951. Protein measurement with the Folin phenol reagent. J biol Chem. 193(1), 265-275.
    17.
  17. Mahdi L., Sana’a A.K., Zwain L., 2015. The effect of Pediococcus pentosaceus bacteriocin on Listeria monocytogenes in soft cheese. In International Conference on Medicinal Genetics, Cellular & Molecular Biology, Pharmaceutical & Food Sciences. Istanbul.
    18.
  18. Sharma N., Kapoor G., Neopaney B., 2006. Characterization of a new bacteriocin produced from a novel isolated strain of Bacillus lentus NG121. Antonie Van Leeuwenhoek. 89(3), 337-343.
    19.
  19. Gupta D., Pushpraj S., 2018. Synthesis, Characterization and biological activity of 4 aminoquinoline 1, 2, 4 triazole conjugated Benzothiazole Derivatives (Doctoral dissertation, Department of Pharmaceutical Sciences Faculty of Health Sciences Sam higginbottom university of agriculture, technology and sciences (formerly allahabad agriculture institute) allahabad-211007 2018).‏
    20.
  20. Harley J., Prescott H., 2002. Laboratory Exercises in Microbiology, 5th Edition. The McGraw−Hill Companies. New York.
    21.
  21. Hussein H.N., Al-Mathkhury H.J.F., Sabbah A.M., 2013. Imipenem-Resistant Acinetobacter baumannii isolated from patients and hospitals environment in Baghdad. Iraqi Journal of Science. 54(4), 803-812.
    22.
  22. Al-Khafaji S.M.S., 2006. Study on capsule of Acinetobacter baumannii and its effect on Immune Response. PH.D. Thesis. Biology department. College of Sciences. AL-Mustansiriyah University.
    23.
  23. Al-Sudani I.A.R., 2018. Effect of New Hydantoin Derivatives on Acinetobacter baumannii and Cancer Cell Line. Ph.D. thesis. University of Mustansiriyah. Baghdad. Iraq.
    24.
  24. Lukovic B., Gajic I., Dimkic I., Kekic D., Zornic S., Pozder T., Radisavljevic S., Opavski N., Kojic M., Ranin L., 2020. The first nationwide multicenter study of Acinetobacter baumannii recovered in Serbia: emergence of OXA-72, OXA-23 and NDM-1-producing isolates. Antimicrobial Resistance & Infection Control. 9(1), 1-12.
    25.
  25. Abdulla A.A., ALthahab A.A., Abed T.A., Mahdi R.K., Fadhil S., 2015. Screening of virulence factors in Acintobacter baumannii isolated from clinical samples. Int J Curr Res Aca Rev. 3(6), 128-134.
    26.
  26. Zhu W., Wang Y., Cao W., Cao S., Zhang J., 2018. In vitro evaluation of antimicrobial combinations against imipenem-resistant Acinetobacter baumannii of different MICs. Journal of Infection and Public Health. 11(6), 856-860.
    27.
  27. Todorov S.D., 2009. Bacteriocins from Lactobacillus plantarum–Production Genetic Organization and Mode of Action. J. Brazilian Journal of Microbiology. 40, 209-221.
    28.
  28. Al-Charrakha A.H., Yousif S.Y., Al- Janabi H.S., 2011. Antimicrobial spectrum of the action of bacteriocins from Klebsiella isolates from Hilla/Iraq. J Microbial. 2(5), 1-11.
    29.
  29. Sarika A.R., Lipton A.P., Aishwarya M.S., 2010. Bacteriocin production by a new isolate of Lactobacillus rhamnosus GP1 under different culture conditions. Adv J Food Sci Technol. 2(5), 291-297.
    30.
  30. Han B., Yu Z., Liu B., Ma Q., Zhang R., 2011. Optimization of bacteriocin production by Lactobacillus plantarum YJG, isolated from the mucosa of the gut of healthy chickens. African Journal of Microbiology Research. 5(10), 1147-1155.
    31.
  31. Bhatt S.M., 2011. Enzymology and Enzymatic Technology. S. Chand Higher Acadamic. chapter 4, 92.
    32.
  32. Andrews P., 1964. Estimation of the molecular weights of proteins by sephadex gel-filtration. Biochem J. 91, 222-232.
    33.
  33. Rubaye H.S.J., 2019. Antibacterial, antibiofilm, immunomodulators and histopathological effect of purified characterized salivaricin against Pseudomonas aeruginosa. M.Sc. thesis. Al-Mustansiriyah University, College of Science, Department of Biology.
    34.
  34. Lafta A., 2010. Klebocin effect partially purified from bacteria Klebsiella pneumoniae isolated locally on some intestinal bacteria and some immune cells. M.Sc. thesis. Al-Mustansiriyah University, College of Science, Department of Biology.
    35.
  35. Abdulsada, 2021. Purification, characterization and antibacterial effect of klebocin and typing of producing klebsiella pneumoniae . M.Sc. thesis. Al-Mustansiriyah University, College of Science, Department of Biology.
    36.
  36. Kaewklom S., Lumlert S., Kraikul W., Aunpad R., 2013. Control of Listeria monocytogenes on sliced bologna sausage using a novel bacteriocin, amysin, produced by Bacillus amyloliquefaciens isolated from Thai shrimp paste (Kapi). Food Control. 32(2), 552-557.
    37.
  37. Kayalvizhi N., Rameshkumar N., Gunasekaran P., 2016. Cloning and characterization of mersacidin like bacteriocin from Bacillus licheniformis MKU3 in Escherichia coli. Journal of Food Science and Technology. 53(5), 2298-2306.
    38.
  38. Therdtatha P., Tandumrongpong C., Pilasombut K., Matsusaki H., Keawsompong S., Nitisinprasert S., 2016. Characterization of antimicrobial substance from Lactobacillus salivarius KL-D4 and its application as biopreservative for creamy filling. Springer Plus. 5(1), 1-21.
    39.

سند

Sanad is a platform for managing Azad University publications

الروابط

المراكز ذات الصلة

دعامة

الصفحات الرسمية

حقوق هذا الموقع الإلكتروني مملوكة لنظام SANAD Press Management. © 1442-1446

الصفحة الرئيسية| دخول| معلومات عنا| اتصل بنا|
[English] [فارسی] [en] [fa]