• الصفحة الرئيسية
  • Effect of 12-week Aerobic Exercise on the Tumor Size and Expression of HIF-1α, BCL-2, Mir-15a, and VEGF Genes in BALB/C Female Mice with Breast Cancer

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عنوان URL للمقالة


رقم المقالة : JCHR-2302-1692 زيارة : 183 الصفحة: 283 - 290

10.22034/jchr.2023.1981082.1692

نوع المخطوط: ابحاث

Effect of 12-week Aerobic Exercise on the Tumor Size and Expression of HIF-1α, BCL-2, Mir-15a, and VEGF Genes in BALB/C Female Mice with Breast Cancer

الموضوعات :

Shaima Banoon 1 (Department of Biology, College of Science, University of Misan, Maysan, Iraq)
Saade Abdalkareem Jasim 2 (Biotechnology Department, College of Applied Science, University of Fallujah, Anbar, Iraq)
Abdolmajid Ghasemian 3 (Noncommunicable Diseases Research Center, Fasa University of Medical Sciences, Fasa, Iran)

تاريخ الإرسال : 08 الثلاثاء , ذو القعدة, 1443 تاريخ التأكيد : 05 السبت , ربيع الأول, 1444 تاريخ الإصدار : 12 الخميس , ذو القعدة, 1444

الکلمات المفتاحية: Exercise, breast cancer, BALB/c mice, VEGF, Bcl-2, HIF-1α, Mir-15a,

ملخص المقالة :

Angiogenesis and mortality are associated with breast cancer, one of the most common tumors in women. Tumor angiogenesis is affected by exercise. This study examined the effects of 12 weeks of exercise on HIF-1α, mir-15a, BCL-2, and VEGF gene expression in BALB/c female mice. Forty BALB/c (two week’s age) female mice with a mean weight of 17±0.21g were separated into control and treadmill aerobic training groups. Mice developed malignant after receiving 200μL of MC4-L2 cells subcutaneously. Running at 15-20m min-1 for 10 weeks was the aerobic activity. Afterward, mice were killed and tumor tissue RNA was extracted. HIF-1α, mir-15a, and VEGF gene expression in BALB/c female mice were measured using quantitative real-time PCR (RT-qPCR). After 12 weeks of exercise, miR-15a expression rose 2.6 fold, whereas HIF-1α, Bcl-2, and VEGF gene expression dropped 3.1, 2.6, and 2.4 fold, respectively (p<0.05). Exercise can activate pathways that slow breast cancer progression. More research is needed to confirm these findings and other molecular pathways.

المصادر:


  1. Lei S., Zheng R., Zhang S., Wang S., Chen R., Sun K., Zeng H., Zhou J., Wei W., 2021. Global patterns of breast cancer incidence and mortality: A population based cancer registry data analysis from 2000 to 2020. Cancer Communications. 41(11), 1183-1194.
    2.
  2. Bray F., Ferlay J., Soerjomataram I., Siegel R. L., Torre L.A., Jemal A., 2018. Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA: A Cancer Journal for Clinicians. 68(6), 394-424.
    3.
  3. Riggio A.I., Varley K. E., Welm A.L., 2021. The lingering mysteries of metastatic recurrence in breast cancer. British Journal of Cancer. 124(1), 13-26.
    4.
  4. Garcia-Martinez L., Zhang Y., Nakata Y., Chan H. L., Morey L., 2021. Epigenetic mechanisms in breast cancer therapy and resistance. Nature Communications. 12(1), 1786.
    5.
  5. Coghill A.E., Engels E.A., Schymura M.J., Mahale P., Shiels M.S., 2018. Risk of breast, prostate, and colorectal cancer diagnoses among HIV-infected individuals in the United States. JNCI: Journal of the National Cancer Institute. 110(9), 959-966.
    6.
  6. Rezende L.F., Murata E., Giannichi B., Tomita L.Y., Wagner G.A., Sanchez Z.M., Celis-Morales C., Ferrari G., 2020. Cancer cases and deaths attributable to lifestyle risk factors in Chile. BMC Cancer. 20(1), 1-14.
    7.
  7. Feng Y., Spezia M., Huang S., Yuan C., Zeng Z., Zhang L., Ji X., Liu W., Huang B., Luo W., 2018. Breast cancer development and progression: Risk factors, cancer stem cells, signaling pathways, genomics, and molecular pathogenesis. Genes & Diseases. 5(2), 77-106.
    8.
  8. Alizadeh A.M., Shiri S., Farsinejad S., 2014. Metastasis review: from bench to bedside. Tumor Biology. 35, 8483-8523.
    9.
  9. Annett S., Moore G., Robson T., 2020. Obesity and cancer metastasis: molecular and translational perspectives. Cancers. 12(12), 3798.
    10.
  10. Dorling L., Carvalho S., Allen J., Gonzalez-Neira A., Luccarini C., Wahlstrom C., Pooley K. A., Parsons M. T., Fortuno C., Wang Q., 2021. Breast cancer risk genes: association analysis in more than 113,000 women. New England Journal of Medicine. 384(5), 428-439.
    11.
  11. Kontomanolis E.N., Fasoulakis Z., Papamanolis V., Koliantzaki S., Dimopoulos G., Kambas N.J., 2019. The impact of microRNAs in breast cancer angiogenesis and progression. MicroRNA. 8(2), 101-109.
    12.
  12. Duffy A.M., Bouchier-Hayes D.J., Harmey J.H., 2013. Vascular endothelial growth factor (VEGF) and its role in non-endothelial cells: autocrine signalling by VEGF Madame Curie Bioscience Database [Internet]: Landes Bioscience.https://www.ncbi.nlm.nih.gov/books/NBK6482/
    13.
  13. Betof A.S., Dewhirst M.W., Jones L.W., 2013. Effects and potential mechanisms of exercise training on cancer progression: a translational perspective. Brain, Behavior, and Immunity. 30, S75-S87.
    14.
  14. Isanejad A., Alizadeh A.M., Shalamzari S.A., Khodayari H., Khodayari S., Khori V., Khojastehnjad N., 2016. MicroRNA-206, let-7a and microRNA-21 pathways involved in the anti-angiogenesis effects of the interval exercise training and hormone therapy in breast cancer. Life Sciences. 151, 30-40.
    15.
  15. Foo J.B., Yazan L.S., Tor Y.S., Wibowo A., Ismail N., How C.W., Armania N., Loh S.P., Ismail I.S., Cheah Y.K., 2015. Induction of cell cycle arrest and apoptosis by betulinic acid-rich fraction from Dillenia suffruticosa root in MCF-7 cells involved p53/p21 and mitochondrial signalling pathway. Journal of Ethnopharmacology. 166, 270-278.
    16.
  16. Hofmann P., 2018. Cancer and exercise: Warburg hypothesis, tumour metabolism and high-intensity anaerobic exercise. Sports. 6(1), 10.
    17.
  17. Patel J.G., Bhise A.R., 2017. Effect of aerobic exercise on cancer-related fatigue. Indian Journal of Palliative Care. 23(4), 355.
    18.
  18. But-Hadzic J., Dervisevic M., Karpljuk D., Videmsek M., Dervisevic E., Paravlic A., Hadzic V., Tomazin K., 2021. Six-minute walk distance in breast cancer survivors—A systematic review with meta-analysis. International Journal of Environmental Research and Public Health. 18(5), 2591.
    19.
  19. Jia N., Zhou Y., Dong X., Ding M., 2021. The antitumor mechanisms of aerobic exercise: A review of recent preclinical studies. Cancer Medicine. 10(18), 6365-6373.
    20.
  20. Liu J., Yang C., Huang X.M., Lv P.P., Yang Y.K., Zhao J.N., Zhao S.Y., Sun W.J., 2021. Knockdown of fbi-1 inhibits the warburg effect and enhances the sensitivity of hepatocellular carcinoma cells to molecular targeted agents Via mir-3692/Hif-1α. Frontiers in Oncology. 11, 796839.
    21.
  21. Zhou J.D., Zhang T.J., Xu Z.J., Gu Y., Ma J.C., Li X.X., Guo H., Wen X.M., Zhang W., Yang L., 2019. BCL2 overexpression: clinical implication and biological insights in acute myeloid leukemia. Diagnostic Pathology. 14, 1-10.
    22.
  22. Alizadeh A.M., Heydari Z., Rahimi M., Bazgir B., Shirvani H., Alipour S., Heidarian Y., Khalighfard S., Isanejad A., 2018. Oxytocin mediates the beneficial effects of the exercise training on breast cancer. Experimental Physiology. 103(2), 222-235.
    23.
  23. Liu Z., Jiang J., Yang Q., Xiong Y., Zou D., Yang C., Xu J., Zhan H., 2016. MicroRNA-682-mediated downregulation of PTEN in intestinal epithelial cells ameliorates intestinal ischemia–reperfusion injury. Cell Death & Disease. 7(4), e2210-e2210.
    24.
  24. Khalighfard S., Rajabi H., Gharakhanlou R., Khalighfard S., Setoudeh V., Alizadeh A. M., 2018. The effect of 8 weeks of interval aerobic exercise before and after induction of breast cancer on serum level of irisin and tumor growth in balb/c mice. Journal of Isfahan Medical School. 35(459), 1775-1784.
    25.
  25. Rezaei Z., Shakerian S., Nikbakht M., 2019. The Effect of Ten Weeks of High Intensity Interval Training and Continuous Endurance Training on The Expression of MIR-205 and VEGF Gene in Mice with Breast Cancer Has Been Compared. Journal of Applied Health Studies in Sport Physiology. 6(1), 37-44.
    26.
  26. Wahl P., Jansen F., Achtzehn S., Schmitz T., Bloch W., Mester J., Werner N., 2014. Effects of high intensity training and high volume training on endothelial microparticles and angiogenic growth factors. PloS One. 9(4), e96024.
    27.
  27. Mur‐Gimeno E., Postigo‐Martin P., Cantarero‐Villanueva I., Sebio‐Garcia R., 2022. Systematic review of the effect of aquatic therapeutic exercise in breast cancer survivors. European Journal of Cancer Care. 31(1), e13535.
    28.
  28. Brown M.J., Morris M.A., Akam E.C., 2021. An exploration of the role of exercise in modulating breast cancer progression in vitro: a systematic review and meta-analysis. American Journal of Physiology-Cell Physiology. 320(3), C253-C263.
    29.
  29. Otmani K., Lewalle P., 2021. Tumor suppressor miRNA in cancer cells and the tumor microenvironment: mechanism of deregulation and clinical implications. Frontiers in Oncology. https:// doi.org/ 10.3389/ fonc. 2021.708765
    30.
  30. Jalali Z., Shahidi F., 2021. Interactive Effect of 6 Weeks of Aerobic Exercise and Quercetin Supplementation on TIE-2 and VEGF-A Expression in Tumor Tissue of Female Mice with Breast Cancer. Iranian Journal of Breast Diseases. 14(3), 46-56.
    31.
  31. Siemann D.W., Horsman M.R., 2015. Modulation of the tumor vasculature and oxygenation to improve therapy. Pharmacology & Therapeutics. 153, 107-124.
    32.
  32. de la Cruz-López K.G., Castro-Muñoz L.J., Reyes-Hernández D.O., García-Carrancá A., Manzo-Merino J., 2019. Lactate in the regulation of tumor microenvironment and therapeutic approaches. Frontiers in Oncology. 9, 1143.
    33.
  33. Oguntade A.S., Al-Amodi F., Alrumayh A., Alobaida M., Bwalya M., 2021. Anti-angiogenesis in cancer therapeutics: The magic bullet. Journal of the Egyptian National Cancer Institute. 33(1), 1-11.
    34.
  34. Cowman S. J., Koh M. Y., 2022. Revisiting the HIF switch in the tumor and its immune microenvironment. Trends in Cancer. 8(1), 28-42.
    35.
  35. Mclaughlin M., Florida-James G., Ross M., 2021. Breast cancer chemotherapy vascular toxicity: a review of mediating mechanisms and exercise as a potential therapeutic. Vascular Biology. 3(1), R106.
    36.
  36. Peng Y., Croce C. M., 2016. The role of MicroRNAs in human cancer. Signal Transduction and Targeted Therapy. 1(1), 1-9.
    37.
  37. Luo Q., Li X., Li J., Kong X., Zhang J., Chen L., Huang Y., Fang L., 2013. MiR-15a is underexpressed and inhibits the cell cycle by targeting CCNE1 in breast cancer. International Journal of Oncology. 43(4), 1212-1218.
    38.
  38. Li Z., Zhu Z., Wang Y., Wang Y., Li W., Wang Z., Zhou X., Bao Y., 2021. hsa‑miR‑15a‑5p inhibits colon cell carcinoma via targeting CCND1. Molecular Medicine Reports. 24(4), 1-8.
    39.
  39. Ramaiah M.J., 2018. Functions and epigenetic aspects of miR-15/16: Possible future cancer therapeutics. Gene Reports. 12, 149-164.
    40.
  40. Wu B., Liu G., Jin Y., Yang T., Zhang D., Ding L., Zhou F., Pan Y., Wei Y., 2020. miR-15b-5p promotes growth and metastasis in breast cancer by targeting HPSE2. Frontiers in Oncology. 10, 108.
    41.
  41. Lee A.V., Oesterreich S., Davidson N.E., 2015. MCF-7 cells—changing the course of breast cancer research and care for 45 years. JNCI: Journal of the National Cancer Institute. 107(7). DOI: 10.1093/jnci/djv073.
    42.
  42. Lv Q., Wang G., Zhang Y., Shen A., Tang J., Sun Y., Ma C., Wang P., 2021. CircAGAP1 promotes tumor progression by sponging miR-15-5p in clear cell renal cell carcinoma. Journal of Experimental & Clinical Cancer Research. 40(1), 1-14.
    43.
  43. Hong B.S., 2022. Regulation of the Effect of Physical Activity Through MicroRNAs in Breast Cancer. International Journal of Sports Medicine. 43(5), 455-465.
    44.

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