Hepatic Alterations in the Amphibian Sclerophrys regularis as Biomarkers for Habitat Destruction

الموضوعات :

Rashad Said ¹ (Department of Zoology, Faculty of Science, Al-Azhar University, Assiut, Egypt)
Ali G. Gadel Rab ² (Department of Zoology, Faculty of Science, Al-Azhar University, Assiut, Egypt)
Noura M. Khattab ³ (Department of Zoology, Faculty of Science (Girls), Al-Azhar University, Cairo, Egypt)
Samy A. Saber ⁴ (Department of Zoology, Faculty of Science, Al-Azhar University, Cairo, Egypt)
Boshra A. El-Salkh ⁵ (Department of Zoology, Faculty of Science (Girls), Al-Azhar University, Cairo, Egypt)

تاريخ الإرسال : 01 الأربعاء , ربيع الثاني, 1444 تاريخ التأكيد : 18 الثلاثاء , رجب, 1445 تاريخ الإصدار : 06 السبت , رمضان, 1445

الکلمات المفتاحية: Histopathology, Heavy metals, Sclerophrys regularis, Hepatic alterations,

ملخص المقالة :

Heavy metals contamination may have negative impacts on the wetland biota, particularly on amphibians, and result in a severe decline globally. Because they cannot be degraded biologically, heavy metals are difficult to remove from the environment. To assess the histopathological biomarkers, some heavy metals Fe, Mn, Pb, Cd, and Cr were analyzed in the livers of Egyptian toads (Sclerophrys regularis) from North Giza (Abu Rawash) and El Wahat Regions (El Bawiti) as well as histological changes. The findings demonstrated that the heavy metals concentrations in the Egyptian toad liver at El Wahat were lower than those recorded in the El Giza region. In accordance with heavy metals burden, hepatic alterations were demonstrated and described. Because the liver is the organ where all types of toxins and chemicals are detoxified, the severity of the hepatic alterations observed in this study may be related to the damaging effects of environmental pollutants on hepatocytes.

المصادر:

1. Abalaka S.E., 2015. Heavy metals bioaccumulation and histopathological changes in Auchenoglanis occidentalis fish from Tiga dam, Nigeria. Journal of Environmental Health Science and Engineering. 13(1),1-8.


2. Abdel-Moneim A.M., Essawy A.E., El-Din N.K.B., El-Naggar N.M., 2016. Biochemical and histopathological changes in liver of the Nile tilapia from Egyptian polluted lakes. Toxicology and industrial health. 32(3), 457-467.


3. Ali H., Khan E., 2019. Trophic transfer, bioaccumulation, and biomagnification of non-essential hazardous heavy metals and metalloids in food chains/webs—Concepts and implications for wildlife and human health. Human and Ecological Risk Assessment: An International Journal. 25(6),1353-1376.


4. Bancroft J., Stevens A., 1996. Theory and practice of histological techniques. 1996. pp. 185:282.


5. Bryer P.J., 2008. Bioaccumulation and effects of metal contaminated soil on Great Plains toads, Bufo cognatus, Texas Tech University.


6. Çakıcı Ö., 2015. Histopathologic changes in liver and kidney tissues induced by carbaryl in Bufotes variabilis (Anura: Bufonidae). Experimental and Toxicologic Pathology. 67(3), 237-243. doi: https://doi.org/10.1016/j.etp.2014.12.003


7. Collins J.P., 2010. Amphibian decline and extinction: what we know and what we need to learn. Diseases of Aquatic Organisms. 92(2-3), 93-99.


8. Doherty V., Ogunkuade O., Kanife U., 2010. Biomarkers of oxidative stress and heavy metal levels as indicators of environmental pollution in some selected fishes in Lagos, Nigeria. American-Eurasian Journal of Agricultural & Environmental Sciences. 7(3), 359-365.


9. Mahmoud F.A., Gadel-Rab A.G., Said R.E., Saber S.A., ElSalkh B.A., Said A.S. Atia M.M., 2022. Impact of atrazine and nitrate on liver and kidney of egyptian toad Sclerophrys regularis: bioindicator alarming on ecosystem. Acta Scientiarum. Biological Sciences. 44(1).

 

 

doi: 10.4025/actascibiolsci.v44i1.56386

10. Grant E.H.C., Miller D.A., Schmidt B.R., Adams M.J., Amburgey S.M., Chambert T., Cruickshank S.S., Fisher R.N., Green D.M., Hossack B.R., 2016. Quantitative evidence for the effects of multiple drivers on continental-scale amphibian declines. Scientific Reports. 6(1),1-9.


11. Ibrahim A. A., 2013. Amphibians of Egypt: a troubled resource. Basic and Applied Herpetology. 27,107-117.


12. Ikechukwu E.L., Ajeh E.A., 2011. Histopathological alterations in the liver and lungs of Hoplobatrachus occipitalis exposed to sub lethal concentrations of cadmium. Australian Journal of Basic and Applied Sciences. 5,1062-1068.


13. Jayawardena U.A., Angunawela P., Wickramasinghe D.D., Ratnasooriya W.D., Udagama P.V., 2017. Heavy metal–induced toxicity in the Indian green frog: Biochemical and histopathological alterations. Environmental Toxicology and Chemistry. 36(10), 2855-2867.


14. Khattab N.M.A., Saber S.A., El-Salkh B.A., Said R.E.M., 2021. The Efficiency of Sclerophrys regularis as a Bioindicator. Egyptian Academic Journal of Biological Sciences, B. Zoology. 13(1), 91-101. doi: 10.21608/eajbsz.2021.154321


15. Khattab N.M.A., Saber S.A., El-Salkh B.A., Said R.E.M., 2021. Genotoxicity and limbs asymmetry in the Egyptian toad (Sclerophrys regularis) as biomarkers for heavy metals toxicity. Egyptian Journal of Aquatic Biology and Fisheries. 25(4), 705-717. doi: 10.21608/ejabf.2021.194098


16. Ma J., Hao Z., Sun Y., Liu B., Jing W., Du J., Li J., 2022. Heavy metal concentrations differ along wetland-to-grassland soils: A case study in an ecological transition zone in Hulunbuir, Inner Mongolia. Journal of Soils and Sediments. 22(4), 1176-1187.


17. McDaniel W., 1991. Sample preparation procedure for spectrochemical determination of total recoverable elements in biological tissues. In "Revision 1.0 Environmental Monitoring Systems Laboratory" pp. 23-29. EPA.


18. Mukherjee S., Chatterjee N., Sircar A., Maikap S., Singh A., Acharyya S., Paul S., 2022. A Comparative Analysis of Heavy Metal Effects on Medicinal Plants. Applied Biochemistry and Biotechnology, 1-36.


19. Păunescu A., Ponepal C.M., Grigorean V.T., Popescu M., 2012. Histopathological changes in the liver and kidney tissues of marsh frog (Pelophylax ridibundus) induced by the action of Talstar 10EC insecticide. Analele Universitatii din Oradea, Fascicula Biologie. 19(1), 5-10


20. Romanova E.B., Ryabinina E.S., Boryakov A.V., 2021. Heavy Metal Accumulation in the Tissues and Organs of Pelophylax ridibundus (Pallas, 1771) and Pelophylax lessonae (Camerano, 1882) (Amphibia: Ranidae) Inhabiting the Waterbodies of Nizhny Novgorod. Biology Bulletin. 48(10), 1862-1869. doi: 10.1134/S1062359021100228


21. Said R.E.M., Said A.S., Saber S.A., ElSalkh B.A., 2022. Biomarker Responses in Sclerophrys regularis (Anura: Bufonidae) Exposed to Atrazine and Nitrate. Pollution. 8(4), 1387-1397. doi: 10.22059/poll.2022.339894.1386


22. Sánchez-Chardi A., Ribeiro C.A.O., Nadal J., 2009. Metals in liver and kidneys and the effects of chronic exposure to pyrite mine pollution in the shrew Crocidura russula inhabiting the protected wetland of Doñana. Chemosphere 76(3), 387-394.


23. Silva J.P., da Silva D.C., Melo F.T., Giese E.G., Furtado A.P., Santos J.N., 2013. Liver histopathology in the cane toad, Rhinella marina (Amphibia: Bufonidae), induced by Ortleppascaris sp. larvae (Nematoda: Ascarididae). The Journal of Parasitology. 99(2), 250-256.


24. Ward C., Hassan S., Mendonça M., 2009. Accumulation and depuration of trace metals in southern toads, Bufo terrestris, exposed to coal combustion waste. Archives of Environmental Contamination and Toxicology. 56(2), 268-275