Study of Potato (Solanum tuberosum L. Agria Cultivar) Microtuberization and Physiological Properties in Salinity Stress via Tissue Culture Technique
الموضوعات :
Journal of Chemical Health Risks
Nosrat Musavi
1
,
Mostafa Ebadi
2
,
Mehdi Khorshidi
3
,
Nahid Masoudian
4
,
Hossein Hokmabadi
5
1 - Department of Plant Physiology, Damghan Branch, Islamic Azad University, Damghan, Iran
2 - Department of Plant Physiology, Damghan Branch, Islamic Azad University, Damghan, Iran
3 - Assistant Professor, Biology School, Damghan University, Damghan, Iran
4 - Department of Plant Physiology, Damghan Branch, Islamic Azad University, Damghan, Iran
5 - Associate Professor, Agricultural Education and Extension Institute, AREEO, Tehran, Iran
تاريخ الإرسال : 06 الأحد , شعبان, 1444
تاريخ التأكيد : 01 الإثنين , ذو الحجة, 1444
تاريخ الإصدار : 06 الأحد , ربيع الثاني, 1447
الکلمات المفتاحية:
salinity stress,
tissue culture,
Physiological properties,
Potato,
ملخص المقالة :
One of the most important crop plants is potato. Plant tissue culture is a very important technology in plant cultivation. In this investigation, the biochemical characterizes are investigated of potatoes cultivated by the tissue culture method. The lateral and apical buds were separated from the stem. They were sterilized and cultured in Murashige and Skoog culture medium. The concentrations of salt (0, 25, 50, 75 and 100 mM) have been used for salinity. After the growth of buds, the leaves were sampled and the chlorophylls, proline, sugars, protein, and antioxidant enzymes activity such as catalase, superoxide dismutase, guaiacol peroxidase, glutathione reductase and ascorbate peroxidase were evaluated. The results indicate that salt decreased protein and chlorophylls content. Sugars content and fresh weight have also been reduced with salinity, but the content of proline, malondialdehyde, and hydrogen peroxide were increased under salinity stress. Phenolic compounds, anthocyanin content, and antioxidant enzymes activity increased up to 50 mM salinity, but they decreased above this concentration. According to these results, it can be suggested that Agria cultivar potatoes are not recommended in salinity higher than 50 mM.
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