Evaluation of Nitrate and Nitrite Levels in Leafy Vegetables to Assess the Potential Health Risks in Tehran, Iran
الموضوعات :
Sadra Sheykhi Oskouei
1
,
Najmeh Youseftabar Miri
2
,
Maryam Taghdir
3
,
Mohammad Nejatian
4
,
Mojtaba Sepandi
5
,
Sepideh Abbbaszadeh
6
1 - Student Research Committee, Baqiyatallah University of Medical Sciences, Tehran, Iran
2 - Health Research Centre, Life Style Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran
3 - Health Research Centre, Life Style Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran
4 - Health Research Centre, Life Style Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran
5 - Health Research Centre, Life Style Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran
6 - Department of Nutrition and Food Hygiene, Faculty of Health, Baqiyatallah University of Medical Sciences, Tehran, Iran
الکلمات المفتاحية: Nitrate and nitrite level, Target hazard quotient (THQ), Estimated daily intake (EDI), Leafy vegetables,
ملخص المقالة :
The nitrate and nitrite content of four leafy vegetables (parsley, garden cress, coriander, and dill) from different regions of Tehran province, Iran (north, south, east, and west) were determined by spectrophotometer method at 538 nm. The results indicated that the dill had the highest nitrate concentration (402.944 mg kg-1), while the coriander had the highest nitrite concentration (0.672 mg kg-1), and these values were significantly higher than other vegetables (P<0.05). Garden cress had the lowest nitrate and nitrite concentrations, 288.556 and 0.378 mg kg-1, respectively. The estimated daily intake (EDI) and target hazard quotient (THQ) of both nitrate and nitrite were below the standard threshold. The EDI values for nitrate were 0.2216, 0.1980, 0.1746, and 0.1587 (mg kg-1 body weight/day) for dill, coriander, parsley, and garden cress, respectively, indicating that the health risk associated with consuming these vegetables was lower than the standard limit (3.7 mg kg-1body weight /day). The THQ results for nitrate and nitrite samples revealed that they were all lower than 1, implying that the risk level is acceptable for chronic risk health exposure to leafy vegetables. In conclusion, our results showed that the studied vegetables were safe to consumption based on nitrate and nitrite concentrations.
1. WHO, Diet, nutrition, and the prevention of chronic diseases: report of a joint WHO/FAO expert consultation. World Health Organization: 2003, Vol. 916.
2. Kumar D., Kumar, S., Shekhar C., 2020. Nutritional components in green leafy vegetables: A review. JPP. 9(5), 2498-2502.
3. Brkić D., Bošnir J., Bevardi M., Bošković A. G., Miloš S., Lasić D., Krivohlavek A., Racz A., Mojsović–Ćuić A., Trstenjak N. U., 2017. Nitrate in leafy green vegetables and estimated intake. AJTCAM. 14 (3), 31-41.
4. Parks S. E., Irving D. E., Milham P. J., 2012. A critical evaluation of on-farm rapid tests for measuring nitrate in leafy vegetables. Sci Hortic. 134, 1-6.
5.Roohparvar R., Shamspur T., Mostafavi A., Bagheri H., 2018. Indirect ultra-trace determination of nitrate and nitrite in food samples by in-syringe liquid microextraction and electrothermal atomic absorption spectrometry. Microchem J. 142, 135-139.
6. Quijano L., Yusà V., Font G., McAllister C., Torres C. Pardo, O., 2017. Risk assessment and monitoring programme of nitrates through vegetables in the Region of Valencia (Spain). FCT. 100, 42-49.
7. Uddin R., Thakur M.U., Uddin M.Z., Islam G.R., 2021. Study of nitrate levels in fruits and vegetables to assess the potential health risks in Bangladesh. Sci Rep 11(1), 4704.
8. Jana J.C., Moktan P., 2006. Nitrate concentration of leafy vegetables: A survey of nitrite concentrations in retail fresh leafy vegetables from daily markets of different locations. ISABB. J Food and Agric. 3(1), 1-5.
9. Santamaria P., 2006. Nitrate in vegetables: toxicity, content, intake and EC regulation. Journal of the Science of Food and Agriculture. 86(1), 10-17.
10. Ali R.A., Muhammad K. A., Qadir, O. K., 2021. In: , IOP Conference Series: Earth and Environmental Science, IOP Publishing, A survey of Nitrate and Nitrite Contents in Vegetables to Assess The Potential Health Risks in Kurdistan, Iraq. pp. 012065.
11. Pirsaheb M., Rahimian S., Pasdar Y., 2012. Nitrite and Nitrate content of fruits and vegetables in Kermanshah (2010). J Kermanshah Univ Med Sci. 16 (1).
12. Tajdar-Oranj B., Javanmardi F., Parastouei K., Taghdir M., Fathi M., Abbaszadeh S., 2023. Health Risk Assessment of Lead, Cadmium, and Arsenic in Leafy Vegetables in Tehran, Iran: the Concentration Data Study. BiolTrace Elem Res. 1-11.
13. Colla G., Kim H.J., Kyriacou M.C., Rouphael Y., 2018. Nitrate in fruits and vegetables. Sci Hortic. 237, 221-238.
14. Souri M.K., Rashidi M., Kianmehr M.H., 2018. Effects of manure-based urea pellets on growth, yield, and nitrate content in coriander, garden cress, and parsley plants. J Plant Nutr. 41(11), 1405-1413.
15. Tuncay O., Esiyok D., Yagmur B., Okur B., 2011. Yield and quality of garden cress affected by different nitrogen sources and growing period. Afr J Agric Res. 6(3), 608-617.
16. Jalali M., Amini Farsani Z., Ghaffarian Mogharab M.H., Feyzian M., 2021. Potential Health Risk of Nitrate Accumulation in Vegetables Grown in Pol-e Dokhtar County. Journal of Chemical. Health Risks. 11(1), 99-111.
17. Cantliffe D.J., 1972. Nitrate Accumulation in Spinach Grown Under Different Light Intensities1, 2. J Am Soc Hortic. Sci. 97(2), 152-154.
18. Cantliffe D.J., 1972. Nitrate accumulation in vegetable crops as affected by photoperiod and light duration1. J Am Soc Hortic. Sci. 97 (3), 414-418.
19.Mott R., Steward F., 1972. Solute accumulation in plant cells V. An aspect of nutrition and development. Ann Bot. 36(5), 915-937.
20. Qiu W., Wang Z., Huang C., Chen B., Yang R., 2014. Nitrate accumulation in leafy vegetables and its relationship with water. J. Soil Sci. Plant Nutr. 14(4), 761-768.
21. Burns I.G., Zhang K.,Turner M.K., Lynn J., McClement S., Hand P., Pink D., 2011. Genotype and environment effects on nitrate accumulation in a diversity set of lettuce accessions at commercial maturity: the influence of nitrate uptake and assimilation, osmotic interactions and shoot weight and development. J Sci Food Agric. 91 (12), 2217-2233.
22. Razgallah N., Chikh-Rouhou H., Abid G., M’hamdi M., 2017. Identification of differentially expressed putative nitrate transporter genes in lettuce. Int J Veg Sci. 23(5), 390-399.
23. Kmiecik W., Lisiewska Z., Jaworska G., 2002. Effect of biological and agrotechnical factors on the chemical composition of dill (Anethum graveolens L.). EJPAU. 5(1), http://www.ejpau.media.pl/volume5/issue1/food/art-06.html.
24. Soysal Y., 2004. Microwave drying characteristics of parsley. Biosyst Eng. 89(2), 167-173.
25. Doymaz İ., Tugrul N., Pala M., 2006. Drying characteristics of dill and parsley leaves. J Food Eng. 77(3), 559-565.
26. Bhat S., Kaushal P., Kaur M., Sharma H., 2014. Coriander (Coriandrum sativum L.): Processing, nutritional and functional aspects. Afr J Plant Sci. 8(1), 25-33
27. Nowrouz P., Taghipour H., Dastgiri S., Bafandeh Y., Hashemimajd K., 2012. Nitrate determination of vegetables in Varzeghan City, North-western Iran. Health Promot Perspect. 2(2), 244.
28. Kyriacou M.C., Soteriou G.A., Colla G., Rouphael Y., 2019. The occurrence of nitrate and nitrite in Mediterranean fresh salad vegetables and its modulation by preharvest practices and postharvest conditions. Food Chem. 285, 468-477.
29. Tibaldi G., Fontana E., Nicola S., 2009. In: International Symposium Postharvest Pacifica 2009-Pathways to Quality: V International Symposium on Managing Quality in 880, Influence of maturity stage at harvest on essential oil composition of dill leaves (Anethum graveolens L.) and of postharvest treatments on freshness of fresh-cut dill. 261-266.
30. Alharbi B.M., Mahmoud A.A., Astatkie T., Said-Al Ahl H.A.H., 2019. Growth and essential oil composition responses of parsley cultivars to phosphorus fertilization and harvest date. J Plant Nutr. 42(18), 2395-2405.
31. Sarwar, S., Ayyub M.A., Rezgui M., Nisar S., Jilani M.I., 2019, Parsley: A review of habitat, phytochemistry, ethnopharmacology and biological activities. Int J Chem Biochem Sci. 9, 49-55.
32. Guha S., Sharangi A.B., Debnath S., 2014. Phenology and green leaf yield of coriander at different sowing dates and harvesting times. J Food Agric Environ. 12(3), 251-254.
33. Santamaria P., Gonnella M., Elia A., Parente A., Serio F., 2001. Ways of reducing rocket salad nitrate content. Acta Horticulturae. 548, 529-536.
34. Santamaria P., Elia A., Serio F., Todaro E., 1999. A survey of nitrate and oxalate content in fresh vegetables. Journal of the Science of Food and Agriculture. 79(13), 1882-1888.
35. Konstantopoulou E., Kapotis G., Salachas G., Petropoulos S.A., Karapanos I.C., Passam H.C., 2010. Nutritional quality of greenhouse lettuce at harvest and after storage in relation to N application and cultivation season. Sci Hortic. 125(2), 93. e1-93. e5.
36. Koukounaras A., Siomos A.S., Sfakiotakis E., 2007. Postharvest CO2 and ethylene production and quality of rocket (Eruca sativa Mill.) leaves as affected by leaf age and storage temperature. Postharvest Biol Technol. 46(2), 167-173.
37. Shahlaei A., Ansari N.A., Dehkordie F.S., 2007. Evaluation of nitrate and nitrite content of Iran Southern (Ahwaz) vegetables during winter and spring of 2006. Asian J Plant Sci. 6(1), 97-12.
38.Ranasinghe R.A.S.N., Marapana R.A.U.J., 2018. Nitrate and nitrite content of vegetables: A review. JPP. 7(4), 322-328.
39. Afali S., Elahi R., 2014. Measuring nitrate and nitrite concentrations in vegetables, fruits in Shiraz. J Appl SCI Environ Manag. 18 (3), 451-457.
40. FAO/WHO., Nitrate (And Potential Endogenous Formation of N-nitroso Compounds). In WHO Food Additive Organization, W.H., Ed. 2003, Vol. Geneva series, No 50.
41. Dezhangah S., Nazari F., Kamali K., Hosseini M.J., Mehrasbi M.R., 2022. A survey on nitrate level in vegetables to assess the potential health risks in Iran. Int J Food Prop. 25(1), 1958-1973.
42. Mehri F., Heshmati A., Moradi M., Khaneghah A.M., 2021. The concentration and health risk assessment of nitrate in vegetables and fruits samples of Iran. Toxin Rev. 40(4), 1215-1222.
43. Seilsepour M., 2020. Study of nitrate and heavy metal pollutant concentration in soil and edible leafy vegetables and risk assessment of its consumption with hazard quotient index. HPN. 3(2), 145-158.
