Assessment of Potential Human Health Risk of Heavy Metals in Waterleaf (Talinum triangulare) Sold in Major Markets in Calabar, Nigeria

الموضوعات :

Udiba Udiba ¹ , Michael Odey ² , Udeme Udofia ³ , Ekom Akpan ⁴ , John Ama ⁵

1 - Department of Zoology and Environmental Biology, University of Calabar, Calabar, Nigeria
2 - Dept of Biochemistry, Faculty of Basic Medical Sciences, University of Calabar, Nigeria
3 - Department of Zoology and Environmental Biology, University of Calabar, Calabar, Nigeria
4 - Institute of Oceanography, University of Calabar, Calabar, Nigeria
5 - Department of Zoology and Environmental Biology, University of Calabar, Calabar, Nigeria

تاريخ الإرسال : 16 الإثنين , ذو الحجة, 1442 تاريخ التأكيد : 20 الأحد , جمادى الثانية, 1443 تاريخ الإصدار : 09 الأربعاء , شعبان, 1444

الکلمات المفتاحية: Heavy metals, Human health risk, Talinum triangulare, Phyto-nutraceuticals, Safety status,

ملخص المقالة :

Sequel to the high rate at which waterleaf (Talinum triangulare) is consumed in Southern Nigeria and the excellent phytoextraction and bioaccumulation potentials of the plant, an assessment of the safety status and potential human health risk of Lead (Pb), Calcium (Cd), Chromium (Cd) and Nickel (Ni) through consumption of waterleaf sold in major markets in Calabar, Nigeria was carried out between February and April, 2021. A total of 36 composite samples obtained from 180 waterleaf vendors was used for the study. Heavy metals concentrations were determined using Atomic Absorption Spectrophotometer (Shimadzu, Model AA-6800, Japan) after wet digestion. The concentration (Mg Kg-1) ranged from 0.26-0.59 for lead, 0.04-0.42 for cadmium, 0.35-1.02) and 1.35-4.32 for nickel. The mean metals content of edible tissues (leaves and tender stem) of waterleaf were found to be above FAO/WHO permissible limits and the EU maximum Levels for the metals in leafy vegetable except for chromium. The Estimated Daily Intake of the metals were above their respective Recommended Daily Intake and Upper Tolerable Daily Intake except for nickel. The average Target Hazard Quotients were greater than 1.00 except for chromium. The Hazard Index for the respective markets were greater than unity. The study concludes that Talinum triangulare purchased from the markets under study is not safe for human consumption as it poses significant toxicological risk with respect to lead, cadmium, and nickel and chromium intoxication.

المصادر:

1. Ramya V., Patel P., 2019. Health benefits of vegetables. International Journal of Chemical Studies. 7(2), 82-87


2. Dias J.S., 2012. Nutritional Quality and Health Benefits of Vegetables: A Review. Food and Nutrition Sciences 3, 1354-1374. http:// dx.doi. org/10.4236 /fns.2012.310179


3. Dias J.S., 2011. World Importance, Marketing and Trading of Vegetables. Acta Horticulturae. 921, 153-169.


4. Ogungbenle H.N., Akinsola A.F., Olatunde O.C., Lijoka A.O., 2018. Chemical, Functional properties and Amino acid composition of Waterleaf (Talinum triangulare). Chemistry Research Journal. 3(3), 7-16


5. Mestrovic T., 2018. What are Nutraceuticals. https://www.news-medical.net/health/What - are -Nutraceuticals. aspx (Accessed 2021 April 1).


6. Arya M.S., Reshma U.R., Syama S.T., Anaswara S.J., Karishma S., 2019. Nutraceuticals in vegetables: New breeding approaches for nutrition, food and health: A review. Journal of Pharmacognosy and Phytochemistry. 8(1), 677-682


7. Slavin J.L., Lioyd B., 2020. Health Benefits of Fruits and Vegetables. Advances in Nutrition. 3(9), 506-516


8. Isitor S.U., Otunaiya A.O., Iyanda J.O., 2016. Efficiency of Vegetable Marketing in Peri-Urban Areas of Ogun State, Nigeria. Journal of Agricultural Science. 8(3), 67-78


9. Babayemi J.O., Olafimihan O.H., Nwude D.O., 2017. Assessment of Heavy Metals in Waterleaf from Various Sources in Ota, Nigeria. J Appl Sci Environ Manage. 21(6), 1163-1168


10. Swarna J., Ravindhran R., 2013. Pharmacognostical and phytochemical evaluation of Talinum triangulare (jacq.) wild. International Journal of Pharmacy and Pharmaceutical Sciences. 5(2), 249-256


11. Amusat A.I., Adedokun M.A., Tairu H.M., Adaramola K.A., Olabamiji S.O., 2018. Proximate Analysis Phyto -Chemical Screening and Mineral Composition of Water Leaves (Talinum Triangulare) Harvested in Oyo State College of Agriculture and Technology Igboora SSRG International Journal of Agriculture & Environmental Science. 3(2), 7-10

 

 

 

12. Adodo A., 2016. Benefits of waterleaf. https:// thenationonlineng.net /benefits-of-waterleaf / ( Accessed 2021 April 1)


13. Faluyi O., 2020. Health benefits of talinum triangulare (waterleaf). https://punchng.com/health-benefits-of-talinum-triangulare-waterleaf/ (Accessed 2021 April 1)


14. Aja P.M., Okaka A.N.C., Ibiam U.A., Uruku A.J., Onu P.N., 2010. Proximate Analysis of Talinum triangulare (waterleaf) leaves and its softening principles. Pakistan Journal Nutrition. 9(6), 524-526


15. Enete A.A., Okon U.E., 2010. Economics of Waterleaf (Talinum triangulare) Production in Akwa Ibom State, Nigeria, Field Actions Science Reports 4. http://factsreports.revues.org/438 (Accessed 2021 April 1)


16. Rai P.K., Lee S.S., Zhang M., Tsang Y.F., Kim K., 2019. Heavy metals in food crops: Health risks, fate, mechanisms, and management. Environment International.125, 365–385


17. Vaikosen E.N., Alade G.O., 2017. Determination of heavy metals in medicinal plants from the wild and cultivated garden in Wilberforce Island, Niger Delta region, Nigeria. Journal of Pharmacy and Pharmacognosy Research. 5(2), 129-143


18. WHO - World Health Organization, 2005. Quality control methods for medicinal plants materials reused draft update. QAS/05, 131/Rev. 1, 22-28


19. Udiba U.U., Udofia U.U., Akpan E.R., 2020. Concentration and Potential Human Health Hazards of Heavy Metals in Periwinkle (Tympanotonus fuscatus) Purchased from Major Markets in Calabar, Nigeria. Journal of Health and Pollution 10(28), 1-12.


20. Population Stat, Calabar, Nigeria population. Population Stat. https:// populationstat.com /nigeria/ calabar (Accessed 2020 October 10)


21. Udofia U.U., Udiba U.U., Udofia L.E., Ezike N.N., Udiba S.U., 2016. Assessment of the impact of solid waste dumps on ground water quality, Calabar Municipality, Nigeria. Journal of Advance Research in Biology and Pharmacy Research. 1(4), 18-34


22. Addo M.A., Darko E.O., Gordon B.J., Nyarko B., 2013. Heavy metal montaminations in soil and cassava harvested near cement processing facility in the Volta Region, Ghana: Implications of health risk for the population living in the vicinity. E-journal of science and tech. 3(8), 71-83


23. Njoku-Tony R.F., Udofia H.S., Nwoko C.O., Ihejirika C.E., Ebe T.E., Egbuawa I.O., Ezike M.N., 2020. Heavy metal concentration level in fluted pumpkin (Telfairia occidentalis) grown around Obio/Akpor, Rivers State, Nigeria: Its health implications. Journal of Environmental Science and Public Health. 4(1), 16-31.


24. U.S. Environmental Protection Agency (US EPA), 2011. Exposure Factors Handbook. 2011 Edition (Final Report). National Centre for Environmental Assessment, U.S. Environmental Protection Agency, Washington, DC. Report No. EPA/600/R-09/052F. https://cfpub. epa.gov/ncea/risk/recordisplay.cfm?deid=236252.10-57


25. Omimakinde A.J., Oguntimehin I., Omimakinde E.A., Olaniran O., 2018. Comparison of the proximate and some selected phytochemicals composition of fluted pumpkin (Telfairia occidentalis) leaves and pods. Int Biol Biomed J Autumn. 4(4), 206-212


26. Guerra F., Trevizam A.R., Muraoka T., Marcante N.C., Canniatti-Brazaca S.G., 2012. Heavy metals in vegetables and potential risk for human health. Science Agricultural. 69(1), 54-60


27. U.S. Environmental Protection Agency (US EPA), 2010. Integrated risk information system. http://cfpub.epa.gov/ncea/iris/compare.cfm (Accessed 2020, October 23)

28 WHO, 2008. Guidelines for drinking-water quality - Volume 1: Recommendations. Third Ed. Inc. first Second addenda 668.

29. Agency for Toxic Substances and Disease Registry (ATSDR), 2019. Toxicological profile for chromium. U.S. Department of Health and Human Services, Atlanta, Georgia, USA.


30. Anyalogbu E.A.A., Anadi C.C., Nweje-Anyalowu P.C., Nnoli M.C., 2017. Use of Waterleaf (Talinum triangulare) in Remediation of Soil Exposed to Heavy Metals: A Green Technology Approach. World Journal of Pharmaceutical and Life Sciences. 3(9), 48-53


31. Hejna M., Gottardo D., Baldi A., Dell’Orto V., Cheli F., Zaninelli M. and Rossi L., 2018. Review: Nutritional ecology of heavy metals. Animal. 1-15


32. Neal A.P., Guilarte T.R., 2012. Mechanisms of Heavy Metal Neurotoxicity: Lead and Manganese. Journal of Drug Metabolism and Toxicology. S5-002


33. Commission Regulation (European Commission-EC) 2015/1005 of 25 June 2015 Amending Regulation (EC) No 1881/2006 as regards maximum levels of lead in certain foodstuffs (Text with EEA relevance L 161/9 - 13)


34. FAO/WHO, 2014. General standards for contaminants and toxins in food and feed (CODEX STAN 193-1995).


35. Adedokun A.H., Njoku K.L., Akinola M.O., Adesuyi A.A., Jolaoso A.O., 2016. Potential human health risk assessment of heavy metals intake via consumption of some leafy vegetables obtained from four market in Lagos Metropolis, Nigeria. J Appl Sci Environ Manage. 20(3), 530-539


36. Abimbola W.A., Akindele S.T., Jokotagba O.A., Agbolade O., 2015. Analysis of Heavy Metals in Vegetables Sold in Ijebu-Igbo, Ijebu North Local Government, Ogun State, Nigeria. International Journal of Scientific and Engineering Research. 6(11), 67-75


37. Adu A.A., Aderinola O.J., Mekuleyi G.O., Adebayo P.O., 2020. Comparative assessment of heavy metals concentration in leaf, stem and root of water leaf (Talinum triangulare) (Jacq Willd, 1799) Grown on the soil of Cassidy’s Dumpsite and Farmland in Ojo, Lagos state. Journal of Medicinal Plants Studies. 8(3), 80-84


38. Kathuria P., 2020. Lead Toxicity. https://emedicine.medscape.com/article/1174752-overview (Accessed 2021 May 16^th)


39. Jaishankar M., Tseten T., Anbalagan N., Mathew B.B., Beeregowda K.N., 2014. Toxicity, mechanism and health effects of some heavy metals. Interdisciplinary Toxicology. 7(2), 60–72


40. Flora S.J.S., Mittal M., Mehta A., 2008. Heavy metal induced oxidative stress and its possible reversal by chelation therapy. Indian Journal of Medical Research. 128, 501–523


41. Szymanski M., 2014. Molecular mechanism of zinc toxicity. Journal of Biotechnology, Computational Biology and Bionanotechnology. 95(2), 137-149


42. NIOSH/CDC, 2015. Reference Blood Lead Levels (BLL) for Adults in the U.S. www.cdc.gov/ niosh/topics/ables/pdfs/Reference%20Blood%20Levels%20for%20Adults-2015-12-18_508.pdf. (Accessed 2021 June 5^th)


43. Fairfax R., (2021) Lead Poisoning and OSHA. https://www.orchse-strategies.com/orc-hse-blog/lead-poisoning/ (Accessed 2021 June 5^th)


44. ACCLPP, 2012. Advisory committee childhood lead poisoning prevention, CDC May 2012. http://www. cdc.gov/nceh /lead / ACCLPP / acclpp _ main . htm. (Accessed 2021 June 5^th)


45. EC, 2014. Commission Regulation (EU) No 488/2014 of 12 May 2014 amending Regulation (EC) No 1881/2006 as regards maximum levels of cadmium in foodstuffs (Text with EEA relevance)


46. Ebong G.A., Etuk H.S. Johnson A.S., 2007. Heavy Metals Accumulation by Talinum triangulare grown on Waste Dumpsites in Uyo Metropolis, Akwa Ibom State, Nigeria. Journal of Applied Sciences. 7, 1404-1409.


47. Alia N., Sardar K., Said M., Salma K., Sadia A., Sadaf S., Toqeer A. and Miklas S., 2015. Toxicity and Bioaccumulation of Heavy Metals in Spinach (Spinacia oleracea) Grown in a Controlled Environment. International Journal Environmental Research. 12, 7400-7416


48. Jibril S.A., Hassan S.A., Ishak C.F., Wahab P.E.M., 2017 Cadmium Toxicity Affects Phytochemicals and Nutrient Elements Composition of Lettuce (Lactuca sativa L.). Hindawi, Advances in Agriculture Volume. 2017, 1-7


49. Rania A., Kumar A., Lal A., Pant M., 2014. Cellular mechanisms of cadmium-induced toxicity: a review. International Journal of Environmental Health Research. 24(4), 378-399


50. US-NAS, 2001. Dietary Reference Intakes for Vitamin A, Vitamin K, Arsenic, Boron, Chromium, Copper, Iodine, Iron, Manganese, Molybdenum, Nickel, Silicon, Vanadium, and Zinc. Available from https://www.ncbi.nlm.nih.gov/books/NBK222329/ (2021 June 1^st )


51. Assem L., Zhu H., 2007. Chromium Toxicological Overview. https://assets.publishing.service.gov.uk/government/uploads/system/uploads/attachment_data/file/338694/Chromium_toxicological_overview.pdf (Accessed 2021 June 14^th)


52. EFSA CONTAM Panel (EFSA Panel on Contaminants in the Food Chain), 2015. Scientific opinion on the risks to public health related to the presence of nickel in food and drinking water. EFSA Journal 2015. 13(2), 4002, 202. doi:10. 2903/j. efsa.2015.4002.


53. Das K.K., Reddy R.C., Bagoji I.B., Das S., Bagali S., Mullur L., Khodnapur J.P., Biradar M.S., 2019. Primary concept of nickel toxicity – an overview. Journal of Basic Clinical Physiology and Pharmacology. 30(2), 141–152


54. Boguski T.K., 2021. Environmental Science and Technology Briefs for Citizens. https:// engg.ksu. edu/CHSR/ outreach / resources / docs / healthrisk . pdf (Accessed 2021 June 14^th)


55. Lanre-Iyanda T.Y., Adekunle I.M., 2012. Assessment of heavy metals and their estimated daily intakes from two commonly consumed foods (kulikuli and Zobo) found in Nigeria. African J Food, Nutrition and Development. 3, 6157-6169