Stress-metabolite Stilbenoids in Vine Trunk of Ojaleshi Grape Variety (Vitis vinifera L.) under Crown Gall Infection
الموضوعات :Marine Bezhuashvili 1 , Luigi Bavaresco 2 , Shorena Kharadze 3 , Magda Surguladze 4 , Giorgi Shoshiashvili 5 , Levan Gagunashvili 6 , Lali Elanidze 7 , Paata Vashakidze 8
1 - Agricultural University of Georgia, Institute of Viticulture and Oenology, Tbilisi, Georgia
2 - DI.PRO.VE.S., Università Cattolica del Sacro Cuore, Piacenza, Italia
3 - Agricultural University of Georgia, Institute of Viticulture and Oenology, Tbilisi, Georgia
4 - Agricultural University of Georgia, Institute of Viticulture and Oenology, Tbilisi, Georgia
5 - Agricultural University of Georgia, Institute of Viticulture and Oenology, Tbilisi, Georgia
6 - Agricultural University of Georgia, Institute of Viticulture and Oenology, Tbilisi, Georgia
7 - Agricultural University of Georgia, Institute of Viticulture and Oenology, Tbilisi, Georgia
8 - Agricultural University of Georgia, Institute of Viticulture and Oenology, Tbilisi, Georgia
الکلمات المفتاحية: Crown Gall, Vine, Ojaleshi, Stilbenoids,
ملخص المقالة :
The trunk stilbenoids of healthy and crown gall infected vines of Ojaleshi variety have been studied and identified as stress-metabolite compounds .Vine samples were taken from a 12-year-old vineyard cultivated on yellow soils type in Martvili region (Western Georgia). The stilbenoids were extracted by ethyl acetate and then separated in a chromatographic column and analyzed by the HPLC/MS method. The following stilbenoids have been detected: cis-piceid, trans-resveratrol, trans-ε-viniferin, cis-miyabenol C, cis-miyabenol. The concentrations were higher in infected vines as compared to the healthy ones. This is the first time that stilbenoids have been investigated in Ojaleshi grape variety.
1. Bezhuashvili M., Kharadze Sh., Surguladze M., Shoshiashvili G., Gagunashvili L., Elanidze L., Tskhvedadze L., Vashakidze P., 2019. Occurrence of stilbenoids in grapevine under Crown gall infection. Annals of Agrarian Science.17(4), 481-487.
2. Bezhuashvili M., Kharadze Sh., Surguladze M., Bavaresco L., Shoshiashvili G., Gagunashvili L., Elanidze L.,Vashakidze. P.,2019. Phytoalexins stilbenoids of V. vinifera L. cvs. Saperavi and Rkatsiteli in relation to crown gall infection. 42nd world congress of Vine and Wine. 15-19 July, Geneva, Svitzerland. Book of abstracts, p.360.
3. Bezhuashvili M., Bavaresco L., Surguladze M., Kharadze Sh., Shoshiashvili G., Gagunashvili L., Elanidze L., Vashakidze P., 2019. Influence of stilbenoids on agrobacterium tumefaciens. IIOABJ. 11(1), 9-12.
4. Bezhuashvili M., Tskhvedadze L., Surguladze M., Shoshiashvili G., Kharadze Sh., Gagunashvili L., Elanidze L., Vashakidze P., 2019. Stress-metabolitesphytoalexins -stilbenoids of grape skin Rkatsiteli variety (Vitis vinifera L.) in condition gray mildew. EurAsian Journal of BioSciences. 13, 1-6.
5. Bezhuashvili M., Bavaresco L., Tskhvedadze L., Surguladze M., Shoshiashvili G., Elanidze L., Gagunashvili L., Vashakidze P., 2020. Change of Phytoalexins- Stilbenoids of grape skin Tsolikouri variety (Vitis vinifera L.) in condition Grey mildew. EurAsian Journal of BioSciences. 14, 243-247.
6. Bezhuashvili M., Tskhvedadze L., Surguladze M., Shoshiashvili G., Gagunashvili L., Elanidze L., Vashakidze P., 2019. Change of Phytoalexin Stilbenoids of Grape Skin Rkatsiteli (Vitis vinifera l.) in Condition of Powdery Mildew. Bioscience Biotechnology Research Communications. 12(5), 146-151.
7. Bezhuashvili M., Tskhvedadze L., Surguladze M., Shoshiashvili G, Elanidze L, Vashakidze P., 2020. Change of phytoalexins -stilbenoids of vine leave Tsitska variety (Vitis vinifera L.) in condition Downy mildew. EurAsian Journal of BoSciences. l(14), 167-171.
8. Gabaston J., Cantos-Villar E., Biais B., Waffo-Teguo P., Renouf E., Corio Costet M.F., Richard T., Mérillon J.M., 2017. Stilbenes from Vitis vinifera L. Waste: A Sustainable Tool for Controlling Plasmopara Viticola. Agric Food Chem. 65(13), 2711–2718.
9. Gindro K., Schnee S., Righi D., Marcourt L., Ebrahimi S.N., Massana Codina J., Voinesco F., Michellod E., Wolfender J.L., Ferreira Queiroz E., 2017. Generation of Antifungal Stilbenes Using the Enzymatic Secretome of Botrytis cinerea. J of Natural Products. 80(4 ),887-898.
10. Bavaresco L., Petegolli D., Cantü E., Fregoni M., G. Chiusa G.,Trevisan M., 1997. Elicitation and accumulation of stilbene phytoalexins in grapevine berries infected by Botrytis cinerea. Vitis. 36(2), 77-83.
11. Bavaresco L., Vezzulli S, Battilani P., Giorni P., Pietri A., Bertuzzi T., 2003. Effect of Ochratoxin A-Producing Aspergilli on Stilbenic Phytoalexin Synthesis in Grapes. J Agric Food Chem. 51(21), 6151-6157.
12. Guebailia H.A., Chira K., Richard T., Mabrouk T., Furiga A., 2006. Hopeaphenol: the first resveratrol tetrammer in wines from North Africa. J Agric Food Chem. 54(25), 9559-9564.