The Study of Cytotoxicity of an Imidazolium Based Ionic Liquid on MCF-7 Cell Line
الموضوعات :
Elham Sajadiyeh
1
(Department of Chemistry, Yazd Branch, Islamic Azad University, Yazd, Irann)
Masoumeh Tabatabaee
2
(Department of Chemistry, Yazd Branch, Islamic Azad University, Yazd, Iran)
SayedMorteza Seifati
3
(Department of Biology, Ashkezar Branch, Islamic Azad University, Ashkezar, Iran)
Zohreh Derikvend
4
(Department of Chemistry, Khorramabad Branch, Islamic Azad University, Khorramabad, Iran)
الکلمات المفتاحية: Cytotoxicity, MCF-7 Cell Line, IImidazolium Ionic Liquids, IC50 values,
ملخص المقالة :
Ionic liquids are liquid in wide range and could be applied in many fields. These compounds can be used as solvents for inorganic and organic synthesis and show biological activities. 1-ethyl-3-Methylimidazolium bromide (1) as an ionic liquid has been synthesized from the reaction of 1-methylimidazole and ethylbromide in toluene as solvent and under refluxed condition. The prepared compound was characterized by 1H-NMR, IR spectroscopy and elemental analyses. The effect of cytotoxicity of -ethyl-3-Methylimidazolium bromide (ion liquid)on the MCF-7 cell line has been investigated. The cytotoxic activity of synthesis compound was assessed by the MTT colorimetric method in vitro assay performed in human MCF-7 breast carcinoma cell line. Dose effect of compound was investigated and results showed that the cytotoxic effect was dose-dependent. The decrease of concentration of ion liquid was followed by markedly increase of tumor cell viability. The cytotoxicity of 1-ethyl-3-Methylimidazolium bromide, expressed as IC50values after 48 h incubation. This value was 2.91 mM for mcf-7 cell line.
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