Evaluation of Tetracycline Antibiotic Residue in Honey Samples using ELISA and HPLC
الموضوعات :
Anahita Hoseinpour
1
,
Peyman Ghajarbygi
2
,
Razzagh Mahmoudi
3
,
Reza Norian
4
,
Saeed Shahsavari
5
1 - M.Sc in Food Safety and Hygiene, Student Research Committee, Faculty of Health, Qazvin University of Medical Sciences, Qazvin, Iran .
2 - Associate professor, Health Products Safety Research Center, Qazvin University of medical sciences, Qazvin, Iran
3 - Professor, Medical Microbiology Research Center, Qazvin University of Medical Sciences, Qazvin, Iran
4 - Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
5 - Department of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
الکلمات المفتاحية: Honey, HPLC, Elisa, Antibiotic residues, Tetracycline, Health hazards,
ملخص المقالة :
Honey is used worldwide due to its medicinal and nutritional properties. Antibiotics are used to treat diseases such as American foulbrood and European foulbrood or as a drug for preventing disease in the beehives. Antibiotic residues should be carefully monitored because they can have adverse effects on the general health of human. In this study, the amount of tetracycline residue was measured in honey samples. A total of 80 honey samples were collected from different regions of Qazvin province, Iran. The methods used included enzyme-linked immunosorbent (ELISA) assay and high-performance liquid chromatography (HPLC). ELISA method showed that the maximum and minimum levels of tetracycline residue were 40 ppb and 1.26 ppb, respectively. The areas with values above the kit’s LOD include Takestan (14.28%), Abeyek (4.76%), and Alamot-e-gharbi (4.54%), respectively. In the Alamot-e-sharghi samples, the antibiotic values above the kit’s LOD were not found. Samples with values above the kit’s LOD in ELISA method were measured using HPLC method. According to ELISA results, of the 80 honey samples, 4 samples (5%) had antibiotic more than the highest LOD of the kit. These 4 samples were tested using HPLC method. The results of HPLC showed that out of 4 honey samples, one sample was more than 40 ppb, but 3 samples were less than 40 ppb. There is a significant difference between ELISA and HPLC (p < 0.05). If the antibiotic residue levels of tetracycline are too high in food, it can cause serious harm to the health of consumers, therefore, monitoring of antibiotics residue in food is very necessary.
1. Arabsorkhi B., Sereshti H., 2018. Determination of tetracycline and cefotaxime residues in honey by micro-solid phase extraction based on electrospun nanofibers coupled with HPLC. Microchemical Journal. 140, 241-247.
2. Alvarez-Suarez J.M., Tulipani S., Romandini S., Bertoli E., Battino M., 2010. Contribution of honey in nutrition and human health: a review. Mediterranean Journal of Nutrition and Metabolism. 3(1), 15-23.
3. Kowalczyk E., Sieradzki Z., Kwiatek K., 2018. Determination of pyrrolizidine alkaloids in honey with sensitive gas chromatography-mass spectrometry method. Food Analytical Methods. 11(5), 1345-1355.
4. Mahmoudi R., Norian R., Pajohi-Alamoti M., 2014. Antibiotic residues in Iranian honey by ELISA. International journal of food properties. 17(10), 2367-2373.
5. Carreck N.L., 2018. honey. Taylor & Francis: Journal of Apicultural Research. 57(1), 1-4.
6. El Hawari K., Mokh S., Doumyati S., Al Iskandarani M., Verdon E., 2017. Development and validation of a multiclass method for the determination of antibiotic residues in honey using liquid chromatography-tandem mass spectrometry. Food Additives & Contaminants: Part A. 34(4), 582-597.
7. Mahmoudi R., Moosavy M., Norian R., Kazemi S., Nadari M.R.A., Mardani K., 2014. Detection of oxytetracycline residues in honey samples using ELISA and HPLC methods. Pharmaceutical Sciences. 19(4), 145-150.
8. Aalipour F., Mirlohi M., Jalali M., 2014. Determination of antibiotic consumption index for animal originated foods produced in animal husbandry in Iran, 2010. Journal of Environmental Health Science and Engineering. 12(1), 42-49.
9. Tu C., Dai Y., Xu K., Qi M., Wang W., Wu L., Wang A., 2019. Determination of Tetracycline in Water and Honey by Iron (II, III)/Aptamer-Based Magnetic Solid-Phase Extraction with High-Performance Liquid Chromatography Analysis. Analytical Letters. 52(10), 1-17.
10. Alawad A., Istamboulié G. Calas-Blanchard C., Noguer T., 2019. A reagentless aptasensor based on intrinsic aptamer redox activity for the detection of tetracycline in water. Sensors and Actuators B: Chemical. 288, 141-146.
11. Li J., Wang X., Shan Y., Huang H., Jian D., Xue L., Wang S., Liu F., 2019. Handheld Inkjet Printing Paper Chip Based Smart Tetracycline Detector. Micromachines. 10(1), 27-39.
12. Dabbagh Moghaddam A.; Tayebi L.; Falahatpisheh H., Mahmoudian M., Kowsari N., Akbarein H., Sabzikar A., 2014. Evaluation of the tetracycline residues in pasteurized milks distributed in Tehran by HPLC method. Journal of Army University of Medical Sciences. 11(4), 318-323.
13. Mahmoudi R., Norian R., Gajarbeygi P., 2013. Survey of antibiotic residues in raw milk samples in Qazvin (2012). J Qazvin Univ Med Sci. 18(1), 45-52.
14. Wang G., Zhang H.C., Liu J., Wang J.P., 2019. A receptor-based chemiluminescence enzyme linked immunosorbent assay for determination of tetracyclines in milk. Analytical biochemistry. 564, 40-46.
15. Moudgil P., Bedi J.S., Aulakh R.S., Gill J.P.S., Kumar A., 2019. Validation of HPLC Multi-residue Method for Determination of Fluoroquinolones, Tetracycline, Sulphonamides and Chloramphenicol Residues in Bovine Milk. Food Analytical Methods. 12(2), 338-346.
16. Gunes M., Gunes N., Cibik R., 2009. Oxytetracycline and sulphonamide residues analysis of honey samples from Southern Marmara Region in Turkey. Bulgarian Journal of Agricultural Science. 15(2), 163-167.
17. Saridaki-Papakonstadinou M., Andredakis S., Burriel A., Tsachev I., 2006. Determination of tetracycline residues in Greek honey. Trakia Journal of Sciences. 4(1), 33-36.
18. Vidal J.L.M.N., Aguilera-Luiz M.D.M., Romero-Gonzalez R., Frenich A.G., 2009. Multiclass analysis of antibiotic residues in honey by ultraperformance liquid chromatography− tandem mass spectrometry. Journal of agricultural and food chemistry. 57(5), 1760-1767.
19. Bonvehí J.S., Gutierrez A.L., 2009. Residues of antibiotics and sulfonamides in honeys from Basque Country (NE Spain). Journal of the Science of Food and Agriculture. 89(1), 63-72.
20. Näumann G., Mahrt E., Himmelreich A., Mohring A., Frerichs H., 2012. Traces of contamination–well preserved in honey. Journal für Verbraucherschutz und Lebensmittelsicherheit. 7(1), 35-43.
21. Barrasso R., Bonerba E., Savarino A., Ceci E., Bozzo G., Tantillo G., 2019. Simultaneous Quantitative Detection of Six Families of Antibiotics in Honey Using A Biochip Multi-Array Technology. Veterinary sciences. 6(1), 1-10.
22. Galarini R., Saluti G., Giusepponi D., Rossi R., Moretti S., 2015. Multiclass determination of 27 antibiotics in honey. Food Control. 48, 12-24.
