Histopathology of Inhalation of Industrial Bleach and Detergent Mixture on Epithelial Layer of Trachea in Mice
الموضوعات :
Gh. Vaezi
1
(Semnan Branch, Islamic Azad University, Semnan, Iran)
F. Aliabadi
2
(Department of Biology, Faculty of Science, Damghan Branch, Islamic Azad University,
Damghan, Iran)
Ab. shiravi
3
(Department of Biology, Faculty of Science, Damghan Branch, Islamic Azad University,
Damghan, Iran)
M. pourkazem
4
(Department of Biology, Faculty of Science, Damghan Branch, Islamic Azad University,
Damghan, Iran)
F. Toosi
5
(Department of Biology, Faculty of Science, Damghan Branch, Islamic Azad University,
Damghan, Iran)
الکلمات المفتاحية: Mice, Trachea, chlorine, Bleach, Inhalation, detergents, goblet cell,
ملخص المقالة :
This study is the effects of inhalation toxic mixture of bleach and detergent was examined on the epithelial layer of trachea in the mice. In this study, 42 adult male mice NMRI race weighing 35-40 gr and from age 8 to 10 weeks were divided into 6 experimental groups and one control group. Experimental groups 1-2-3 with the using of chamber, as inhalation 20 minutes were exposure to spray the amount 1 cc of mixture of bleach and detergent by nebulizer. Experimental groups 4-5-6 were for 35 minutes to inhale the same amount of material. Mice killed at 24-48-72 hours after inhalation and the trachea was studied pathology. In microscopic sections of tissue taken from the trachea the experimental group compared with the control group was changed to include: stimulation and activation of the respiratory epithelium hungarian (Mocusa layer), reducing the length of ciliated columnar cells, reducing the number of goblet cells ,loss of cilia, chaos and clutter on the order of tissue. In addition, statistically, the changes in length of ciliated columnar epithelium cells in experimental groups 3 and 6 seen significantly decreased than control group and the number of experimental groups 2 and 4 goblet cells significantly increased compared to control group, experimental group 6 was significantly decreased than the control group. As a result discussion, increasing the inhalation time of mixing bleach and detergent, also as time passed, cause to increase the tissue damage and changes.
- Bagchi K. and S. Puri, 1998. Free radicals and
- anti oxidants inhalation and disease,
- Eastern Mediterranean Health Journal.;
- : 350-360.
- Barrow C.S., Y. Alarie, J. C. Warrick, M.F.
- Stock, 1977. Comparison of the sensory
- irritation response in mice to chlorine and
- hydrogen chloride. Arch Environ Health.
- Mar-Apr; 32(2): 68-76.
- Blanc P.D., M. Galbo, P. Hiatt and K.R. Olson,
- Morbidity following acute irritant
- inhalation in a population based study.
- JAMA,; 266: 664-9.
- Das R. and P.D. Blanc, 1993. Chlorine gas
- exposure and the lung: a review. Toxicol.
- Ind. Health,; 9(3):439-55.
- Dence C. W. and W. Douglas, 1996. Pulp
- Bleaching, Textile Association of Pulp
- and Paper Industry,: 65-69
- Florian M., 2003. The nature and mechanism of
- superoxide production by the electron
- transport chain, Journal of Association of
- Physicians of India,; 4: 227âââ253.
- Gapany-Gapanavicius M., A. Yellin, S. Almog,
- M. Tirosh, 1982. Pneumomediastinum. A
- complication of chlorine exposure from
- mixing household cleaning agents.
- JAMA, Jul 16; 248(3): 349-50.
- Gilchrist H.L. and PB. Matz, 1933 b. The
- residual effects of warfare gases: the use
- of chlorine gas, with report of cases. Med.
- Bull. Vet. Adminis.,; 9: 229-270.
- Grime k. and A. Class, 1990. Laundry Bleaches
- and Activators, Chemistry and
- Industry,:647- 49
- Haghighatpajoh H. and R. Jamshidi, 2006.
- Identifying and detergent technology, first
- printing, Publishing Mobtakeran.: 15-9.
- Han D., E. Williams and E. Cadenas, 2001.
- Mitochondrial respiratory chaindependent
- generation of superoxide anion
- and its release into the intermembrane
- space. Biochem. J, 353: 411âââ6.
- Harman D., 1992. Role of free radicals in aging
- and disease. Annals of New York
- Academy of Sciences, , 673:126-141.
- Hayaishi O., 1969. Enzymatic hydroxylation. Am
- Rev. Biochem, 38: 21-44.
- Hedges J.R. and W.L. Morrissey, 1979. Acute
- chlorine gas exposure. JACEP, Feb; 8(2):
- -63.
- Jiang K., L. Buckley and k. Morgan,
- Pathology of Toxic Responses to
- the RD50Concentration of Chlorine
- Gas in the Nasal Passages of Rats
- and Mice, Toxicol Appl
- Pharmacol;71:225-236
- Jones R.N., J.M. Hughes, H. Glindmeyer and H.
- Weill, 1986. Lung function after acute
- chlorine exposure. Am. Rev. Resp. Dis.,
- Dec; 134(6): 1190-5.
- Klonne D., C. Ulrich, M. Riley, T. Hamm, K.
- Morgan and C. Barrow, One-year
- inhalation toxicology study of chlorine in
- rhesus monkeys, Fundam. Appl. Toxical.,
- : 557-572
- Malcolm,B. H., Poisoning Due to Chlorine Gas,
- CARPIN; 81:42-45
- Martinez T.T. and C. Long, 1995. Explosion risk
- from swimming pool chlorinators and
- review of chlorine toxicity. J. Toxicol.
- Clin. Toxicol.,; 33(4): 349-54.
- Patel, R. P, 2003. The biochemistry of nitric
- oxide and peroxynitrite implications for
- mitochondrial function. In Packer L,
- Cadenas E Understanding the process of
- aging: the roles of mitochondria, free
- radicals, and antioxidants. New York, NY
- Marcel Dekker,; 45:39âââ56.
- Rojhan M., 1992. Color Atlas of Histology and
- practical, first printing, Soroush, 291-309.
- Schwartz D.A. , 1987. Acute inhalational injury.
- Occup. Med., Apr-Jun,; 2(2):297-318.
- Trotman E.R., 1968. Textile scouring and
- bleaching Saudi Public, Textile Scouring
- and Bleaching, 89-94.