Spectrophotometric determination of Dalacin phosphate from formulations using reduce atomic absorption of calcium ion by phosphate
الموضوعات : Journal of the Iranian Chemical Research
Hossein Sheikhloie
1
(Department of Basic Sciences, Maragheh Branch, Islamic Azad University, Maragheh, Iran)
Khalil Farhadi
2
(Department of Chemistry, Urmia University, Urmia, Iran)
الکلمات المفتاحية: Dalacin phosphate, Atomic absorption spectrometry, Pharmaceutical preparation,
ملخص المقالة :
A simple, sensitive and accurate method for spectrophotometric determination of Dalacinphosphatehas been established. The method is based on the reduction of atomic absorptioncalcium ion by phosphate. Because, there is a phosphate mole in every Dalacin mole structureand with measurement of phosphate, Dalacin is beyond measurement too. Therefore, the reactionbetween acidic hydrolysis products of antibiotics with the mixture of calcium ion evaluated forthe spectrophotometric determination of mentioned drug in formulations. Experimentalparameters related to the performance of atiomic absorption spectrometry (AAS), such asconcentration of calcium ion, reaction time and temperature were investigated and optimized.Under the optimized conditions, the limit of detection (3σB) and relative standard deviation(RSD, n=4) were 1.75 μg mL-1 and 1.4 %, respectively. The results obtained agreed with thoseobtained by the USP method.
[1] D.W. Boothe, Small Animal Clinical Pharmacology and Therapeutics, W.B. Saunders Company,
Philadelphia, 2001.
[2] J.F. Prescott, J.D. Baggot, R.D. Walker, Antimicrobial Therapy in Veterinary Medicine, third ed.,
Iowa State University Press, Ames, Iowa, 2000.
[3] F.A. El-Yazbi, S.M. Blaih, Analyst 118 (1993) 577-579.
[4] H. Wan, A.G. Holmen, Y.D. Wang, Rapid Commun. Mass Spectrom. 17 (2003) 2639-2648.
[5] G. Gatti, J. Flaherty, J. bubp, J. White, M. Borin, J. Gambertoglio, J. Antimicrob. Agents Chemother.
37 (1993) 1137-1141.
[6] P.R. Tiller, L.A. Romanyshyn, U.D. Neue, Anal. Bioanal. Chem. 377 (2003) 788-802.
[7] S. Croubels, S. De Baere, P. De Backer, Anal. Chim. Acta 483 (2003) 419-427.
[8] H. F. Büschges, G. Schüßler, V. Larsimont, H. Blume, J. Chromatogr.B 724 (1999) 281-286.
[9] R.N. Rao, V. Nagaraju, J. Pharm. Biomed. Anal. 33 (2003) 335-377.
[10] G.C. Batzias, G.A. Delis, M.K. Papadopoulou, J. Pharm. Biomed. Anal. 35 (2004) 545-554.
[11] Y.R. Ye, E. Bektic, R. Buchta, J. Sep. Sci. 27 (2004) 71-77.
[12] M.A. Targove, N.D. Danielson, J. Chromatogr. Sci. 28 (1990) 505-509.
[13] X. Shao, X. Xie, Y. Liu, Z. Song, J. Pharm. Biomed. Anal. 41 (2006) 667-670.
[14] C. Liu, Y. Chen, T.Yang, S. Hsieh, M.Hung, E.T. Lin. J. Chromatogr. B 696 (1997) 298-305.
[15] H. Nassery, G. Sabzy, Biomed. Chromatog. 19 (2006) 783-787.
