Impact of Heavy Metal Stress on In Vitro Seed Germination and Seedling Growth Indices of Two Turfgrass species
الموضوعات :
Mina Taghizadeh
1
(Department of Horticulture, Faculty of Agriculture and Natural Resources, Arak University, Arak, Iran, ‎P.O.Box:38156-8- 8349‎)
Eisa Solgi
2
(Department of Environment, Faculty of Natural Resources and Environment, Malayer University, ‎Malayer, Hamedan, Iran, P.O. Box: 65719-95863‎)
الکلمات المفتاحية: ryegrass, Copper, Zinc, Cobalt, Fescue,
ملخص المقالة :
Turfgrass is one of the important components of ornamental plant in the construction of parks and landscapes so that the purpose of this study was to investigate the influence of type and different concentrations of Copper, Zinc and Cobalt on germination and seedling growth of perennial ryegrass (Lolium perenne L.) and red fescue (Festuca rubra L.) seeds in in vitro conditions. This study was carried out in 2014. A factorial experiment was conducted based on a completely randomized design with three replications in laboratory. Seeds were sown in the sterilized medium containing 7 g/L of agar under the laminar air flow. Then, three solutions of ZnSO4, CuSO4 and COCl2 in five concentrations of 0, 20, 50, 100 and 200 mg/L were prepared. Seeds were placed in the incubator at 24°C and 50% humidity for 25 days. Seed germination traits and seedling growth were recorded. Results showed that zinc and copper had minimum and maximum inhibitory effects on seedling growth, respectively. At the presence of copper, by increasing the concentration from 0 to 200 mg/L, the germination and seeding growth were sharply reduced. Inhibitory effects on seedling growth were found in higher concentrations than 50 mg/L at the presence of zinc and cobalt metals. The minimum rate of germination and seedling growth in both red fescue and perennial ryegrass was observed in the concentrations of 20 to 200 mg/L of copper. Therefore, perennial ryegrass and red fescue as turfgrass cannot germinate and grow in soils with copper contamination even at low concentrations, but they can tolerate moderate concentration zinc and particularly cobalt contamination in the polluted environments.
Al-Anbari, R., Al Obaidy, A.HM.J., Abd Ali, F.H., 2015. Pollution Loads and Ecological Risk Assessment of Heavy Metals in the Urban Soil Affected by Various anthropogenic Activities. International Jour. Advanced Research, 3(2): 104-110. (In Persian).
Ashagre, H., Almaw, D., Feyisa, T., 2013. Effect of copper and zinc on seed germination, phytotoxicity, tolerance and seedling vigor of tomato (Lycopersicon esculentum L.) cultivar Roma VF. International Journal of Agricultural Science Research, 2(11): 312-317.
Atici, Ö., Ağar, G., Battal, P., 2005. Changes in phytohormone contents in chickpea seeds germinating under lead or zinc stress, Biologia Plantarum, 49(2): 215-222.
Ayaz, F., Kadioglu, A., 1997. Effects of heavy metals (Zn, Cd, Cu, Hg) on the soluble protein bands of germinating Lens esculenta L. seeds. Turkish Jour. Botany, 21: 85-88.
Aydinalp, C., Marinova, S., 2009. The Effects of Heavy Metals on Seed Germination and Plant Growth on Alfalfa Plant (Medicago sativa). Bulgarian Jour. Agricultural Science, 15: 347-350.
De Knecht, J. A., Van Baren, N., Ten Bookum, W. M, Wong Fong Sang, H. W., Koevoets, P. L. M., Schat, H., Verkleij, J. A. C., 1995. Synthesis and degradation of phytochelatins in cadmium-sensitive and cadmium-tolerant Silene vulgaris. Plant Science, 106: 9–18.
Ebrahimi, M., 2012. Germination, Growth and Uptake of Heavy Metals in Contaminated Soils (Hordeum bulbosum L.). Jour. Rangeland Science, 2(3): 557-566. (In Persian).
Ebrahimi, M., Madrid Díaz, F., 2014. Use of Festuca ovina L. in Chelate Assisted Phytoextraction of Copper Contaminated Soils. Jour. Rangeland Science, 4(3): 171-182.
Espen, L., Pirovano, L., Sergio, M.C., 1997. Effect of nickel during the early phases of radish (Raphanus sativus) seed germination. Environmental and Experimental Botany, 38:187–197.
Gangaiah, R. B. A., Chandrasekhar. T., Varaprasad, D., Hima Bindu, Y., Keerthi Kumari, M., Chakradhar, T., Madhava Reddy, C., 2013. Effects of heavy metals on in vitro seed germination and early seedling growth of Pennisetum glacum (L.). International Jour. Food, Agriculture and Veterinary Sciences, 3(3):87-93.
Hall, J. L., 2002. Cellular mechanisms for heavy metal detoxification and tolerance. Jour. Experimental Botany, 53: 1-11.
Kabir, L., Iqbal, M., Shafiq, M.Z., Farooqi, Z.R., 2010. Effects of lead on seedling growth of Thespesia populnea. Plant, Soil and Environment, 56 (4): 194–199
Mahmood, T., Islam, K.R., Muhammad, S., 2007. Toxic effects of heavy metals on early growth and tolerance of cereal crops. Pakistan Jour. Botany, 39(2): 451-462.
Mishra, A., Choudhuri, M. A., 1999. Effects of salicylic acid on heavy metal-induced membrane deterioration mediated by lipoxygenase in rice. Biologia Plantarum, 42:409–415.
Moraghan, J. T., Grafton, K., 1999. Seed-zinc concentration and the zinc-efficiency trait in navy bean. Soil Science Society of America Jour., 63: 918-922.
Munzuroglu, O. and Geckil, H., 2002. Effects of Metals on Seed Germination, Root Elongation, and Coleoptile and Hypocotyls Growth in Triticum aestivum and Cucumis sativus. Archives of Environment Contamination and Toxicology, 43: 203-213
Ouariti, O., Boussama, A., Zarrouk, M., Cherif, A., Ghorbal, M. H., 1997. Cadmium- and copper-induced changes in tomato membrane lipids. Phytochemistry, 45: 1343–1350
Peralta, J. R., Gardea-Torresdey, J. L., Tiemann, K. J., 2000. Study of the effects of heavy metals on seed germination and plant growth on alfalfa plant (Medicago sativa) grown in solid media. Proceedings of the 2000 Conference on Hazardous Waste Research, Denver, Colorado, May 23-25, 2000. pp: 135-140.
Rout, G. R., Das, P., 2003. Effect of metal toxicity on plant growth and metabolism: I. Zinc. Agronomie, 23: 3-11.
Sharma, R. K., Agrawal, M., Marshall, F. M., 2009. Heavy metals in vegetables collected from production and market sites of a tropical urban area of India. Food and Chemical Toxicology, 47: 583-591.
Stefanov, K., Seizova, K., Yanishlieva, N., Marinova, E., Popov, S., 1995. Accumulation of Pb, Zn and Cd in plant seeds growing in metalliferous habitats in Bulgaria. Food Chemistry, 54: 311-313.
Taghizadeh, M., Kafi, M., Fattahi Moghaddam, M. R., Savaghebi, G. H., 2002. Lead concentrations effect on seed germination of turfgrass genius and its evaluation potential for phyto remediation. Iranian Jour. Horticultural Science, 42(3): 277-289. (In Persian).
Tucker, M. R., Hardy, D. H., Stokes, C. E., 2005. Heavy metals in North Carolina soils. Occurrence and Significance Raleigh (NC). North Carolina Department of Agriculture and Consumer Services, Agronomic Division, pp: 2.
Vassilev, A., Yordanov, I., 1997. Reductive analysis of factors limiting growth of cadmium treated plants. Bulg Jour. Plant Physiol, 23(3-4): 114-133.
Wierzbicka, M. and Obidziniska, J., 1988. The effect of lead on seed imbibitions and germination in different plant species. Plant Science, 137: 155-171.
Wong, M. H., Bradshaw, A. D., 1982. A comparison of the toxicity of heavy metals, using root elongation of ryegrass (Lolium perenne). New Phytol, 91:255–261.
Xa, S., Li, J., Zhang, X., Wei, H., Cui, L., 2006. Effects of heat acclimation pretreatment on changes of membrane lipid peroxidation, antioxidant metabolites, and ultrastructure of chloroplasts in two cool-season turfgrass species under heat stress. Environmental and Experimental Botany, 56(3): 274-285.
Xiong, Z. T., 1998. Lead uptake and effects on seed germination and plant growth in a Pb hyper-accumulator Brassica pekinensis Rupr. Bulletin of Environmental Contamination and Toxicology, 60: 285-291.
