بررسی آزمایشگاهی بارگذاری بهینه توکسیدهای دیفتری و کزاز روی نانوذرات آلیژینات
الموضوعات :
سیمین حسینی
1
,
مجتبی نوفلی
2
,
مهروز دزفولیان
3
,
حمیدرضا گودرزی
4
,
زهرا صالحی نجف آبادی
5
1 - دانشجوی دکتری باکتری شناسی، دانشگاه آزاد واحد کرج، دانشکده زیست، گروه باکتری شناسی، کرج، ایران.
2 - دانشیار پزشکی، موسسه تحقیقات واکسن و سرم سازی رازی، سازمان تحقیقات، آموزش و توسعه کشاورزی، گروه تولید واکسن‌های باکتریایی انسانی،
3 - استادیار ژنتیک مولکولی، دانشگاه آزاد . واحد کرج، دانشکده زیست، گروه باکتری شناسی، کرج، ایران.
4 - آزمایشگاه مرکزی، موئسسه سرم و واکسن رازی، سازمان تحقیقات، آموزش و توسعه کشاورزی، کرج، ایران
5 - آزمایشگاه مرکزی، موئسسه سرم و واکسن رازی، سازمان تحقیقات، آموزش و توسعه کشاورزی، کرج، ایران
تاريخ الإرسال : 28 الجمعة , رمضان, 1443
تاريخ التأكيد : 28 الجمعة , محرم, 1444
تاريخ الإصدار : 10 الثلاثاء , صفر, 1444
الکلمات المفتاحية:
پتانسیل زتا,
توکسوئید دیفتری,
هم-انتقالی,
بازدهی بارگذاری,
ادجونت,
ملخص المقالة :
سابقه و هدف: واکسن دیفتری و کزاز (DT) حاوی مقدار بالایی آلومینیوم به عنوان ادجونت است که پتانسیل تأثیر بر سیستم عصبی، به ویژه در نوزادان با بیماری های کلیوی را دارد. در اینجا هدف آماده سازی آزمایشگاهی و ارزیابی تحویل توکسوئیدهای دیفتری و کزاز با بارگذاری روی نانوذرات (NPs) بود.مواد و روش ها: با استفاده از روش یونیزاسیون-ژل، نمونه های نانوذرات آلژینات سدیم تهیه و از نظر اندازه، پتانسیل زتا و شاخص پراکندگی (PDI) ارزیابی شدند. اثرات غلظت آلژینات، کلرید کلسیم و پلی ال-لایزین و سرعت و زمان هم زدن به همراه بازده بارگیری، ظرفیت بارگیری و مشخصات آزادسازی در شرایط آزمایشگاهی بررسی گردید.یافته ها: نانوذرات بهینه شده در غلظت آلژینات سدیمw/v 0/2%، کلرید کلسیم w/v 0/1% و پلی ال -لایزین w/v 0/4% طی ۴۵ دقیقه هم زدن در ۱۳۰۰ دور در دقیقه تهیه شدند. آن ها همچنین دارای اندازه متوسط ذرات کوچک تر از ۱۵۰ نانومتر با PDI حدود 0/5 بودند. بازده بارگیری مناسب با غلظت های توکسوئیدهای بارگذاری شده مشابه واکسن DT رایج به دست آمد که منجر به آزادسازی طولانی مدت حدود 85% سموم بارگذاری شده در طی ۱۲۰ ساعت شد. همچنین، نتایجSDS-PAGE وdot-blot فعالیت آنتی ژنی توکسوئیدهای آزاد شده را تأیید کردند.نتیجه گیری: این نتایج می تواند به میزان قابل توجهی به توسعه بیشتر فناوری نانوذرات آلژینات حاوی توکسوئیدهای DT در شرایط بهینه in vitro کمک نماید تا به عنوان بستری برای ارزیابی بیشتر به صورت درون تن به منظور دستیابی به ابزاری نویدبخش برای ایمن سازی نوزادان و کودکان در برابر دیفتری و کزاز باشد.
المصادر:
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Apostólico JdS, Lunardelli VAS, Coirada FC, Boscardin SB, Rosa DS. Adjuvants: classification, modus operandi, and licensing. J Immunol Res. 2016;2016.
Prashant CK, Kumar M, Dinda AK. Nanoparticle based tailoring of adjuvant function: the role in vaccine development. J Biomed Nanotechnol. 2014;10(9):2317-31.
Tomljenovic L, Shaw C. Mechanisms of aluminum adjuvant toxicity and autoimmunity in pediatric populations. Lupus. 2012;21(2):223-30.
Principi N, Esposito S. Aluminum in vaccines: Does it create a safety problem? Vaccine. 2018;36(39):5825-31.
Esposito S, Mastrolia MV, Prada E, Pietrasanta C, Principi N. Vaccine administration in children with chronic kidney disease. Vaccine. 2014;32(49):6601-6.
Chen Y-C, Cheng H-F, Yang Y-C, Yeh M-K. Nanotechnologies applied in biomedical vaccines. Micro and Nanotechnologies for Biotechnology. Stanciu SG. London. IntechOpen. (2016): 84-105.
Lung P, Yang J, Li Q. Nanoparticle formulated vaccines: opportunities and challenges. Nanoscale. 2020;12(10):5746-63.
Ching SH, Bansal N, Bhandari B. Alginate gel particles–A review of production techniques and physical properties. Crit Rev Food Sci Nutr. 2017;57(6):1133-52.
Rajaonarivony M, Vauthier C, Couarraze G, Puisieux F, Couvreur P. Development of a New Drug Carrier Made from Alginate. J Pharm Sci. 1993;82(9):912-7.
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Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976;72(1):248-54.
Rezaei MA, Mortazavi S, Mohammadpour DN, Zou AH, Alonso M. Preparation and in-vitro evaluation of sodium alginate microspheres containing diphtheria toxoid as new vaccine delivery. Arch Razi Inst. 2008; (2):19-28. 14. Papadimitriou S, Bikiaris D, Avgoustakis K, Karavas E, Georgarakis M. Chitosan nanoparticles loaded with dorzolamide and pramipexole. Carbohydr Polym. 2008;73(1):44-54.
Draget KI, Taylor C. Chemical, physical and biological properties of alginates and their biomedical implications. Food Hydrocoll. 2011;25(2):251-6.
Silva CM, Ribeiro AJ, Figueiredo IV, Gonçalves AR, Veiga F. Alginate microspheres prepared by internal gelation: Development and effect on insulin stability. Int J Pharm. 2006;311(1-2):1-10.
Alfatama M, Lim LY, Wong TW. Alginate–C18 conjugate nanoparticles loaded in tripolyphosphate-cross-linked chitosan–oleic acid conjugate-coated calcium alginate beads as oral insulin carrier. Mol Pharm. 2018;15(8):3369-82.
Ghaderinia P, Shapouri R. Assessment of immunogenicity of alginate microparticle containing Brucella melitensis 16M oligo polysaccharide tetanus toxoid conjugate in mouse. Banat's J Biotechnol. 2017;8(16):83-92.
Azimi S, Safari Zanjani L. Immunization against Pseudomonas aeruginosa using Alg-PLGA nano-vaccine. Ir J Basic Med Sci. 2021; 476-482.
Pandey R, Khuller G. Polymer based drug delivery systems for mycobacterial infections. Curr Drug Deliv. 2004;1(3):195-201.
Lin S-F, Chen Y-C, Chen R-N, Chen L-C, Ho H-O, Tsung Y-H, et al. Improving the stability of astaxanthin by microencapsulation in calcium alginate beads. PLoS One. 2016;11(4):e0153685.
Tsai S, Ting Y. Synthesize of alginate/chitosan bilayer nanocarrier by CCD-RSM guided co-axial electrospray: A novel and versatile approach. Food Res Int. 2019;116:1163-72.
González Ferreiro M, Tillman L, Hardee G, Bodmeier R. Characterization of alginate/ poly-L-lysine particles as antisense oligonucleotide carriers. Int J Pharm. 2002;239(1-2):47-59.
Sarei F, Dounighi NM, Zolfagharian H, Khaki P, Bidhendi SM. Alginate nanoparticles as a promising adjuvant and vaccine delivery system. Indian J Pharm Sci. 2013;75(4):442.
Dobakhti F, Rahimi F, Dehpour AR, Taghikhani M, Ajdary S, Rafiei S, et al. Stabilizing effects of calcium alginate microspheres on Mycobacterium bovis BCG intended for oral vaccination. J Microencapsul. 2006;23(8):844-54.26. Zimet P, Mombrú ÁW, Faccio R, Brugnini G, Miraballes I, Rufo C, et al. Optimization and characterization of nisin-loaded alginate-chitosan nanoparticles with antimicrobial activity in lean beef. LWT. 2018;91:107-16.
Baghbani F, Moztarzadeh F, Mohandesi JA, Yazdian F, Mokhtari-Dizaji M, Hamedi S. Formulation design, preparation and characterization of multifunctional alginate stabilized nanodroplets. Int J Biol Macromol. 2016;89:550-8.
Goudarzi HR, Mokarram A, Noofeli M, Shirvan A, Saadati M. Preparation and evaluation of alginate nanoparticles containing pertussis toxin as a particulate delivery system. Int J Adv Biotechnol Res. 2016;7(2):558-64.
Woitiski CB, Veiga F, Ribeiro A, Neufeld R. Design for optimization of nanoparticles integrating biomaterials for orally dosed insulin. Eur J Pharm Biopharm. 2009;73(1):25-33.
Woitiski CB, Neufeld RJ, Ribeiro AJ, Veiga F. Colloidal carrier integrating biomaterials for oral insulin delivery: influence of component formulation on physicochemical and biological parameters. Acta Biomater. 2009;5(7):2475-84.
31.Dórea JG. Exposure to mercury and aluminum in early life: developmental vulnerability as a modifying factor in neurologic and immunologic effects. Int J Env Res Public Health. 2015;12(2):1295-313.
Tarhini M, Benlyamani I, Hamdani S, Agusti G, Fessi H, Greige-Gerges H, et al. Protein-based nanoparticle preparation via nanoprecipitation method. Materials. 2018;11(3):394.
Moradi Bidhendi S, Zolfagharian H, Mohamadpour Dounighi N, Saraei F, Khaki P, Inanlou F. Design and evaluate alginate nanoparticles as a protein delivery system. Arch Razi Inst. 2013;68(2):139-46.
Ferreiro MG, Tillman L, Hardee G, Bodmeier R. Characterization of alginate/poly-L-lysine particles as antisense oligonucleotide carriers. Int J Pharm. 2002;239(1-2):47-59.
Modi K, Bhalodia J, Pathak T, Raval P, Pansara P, Vasoya N, et al. Bimodal to Unimodal Particle Size Distribution Transformation in Nanocrystalline Cobalt–Ferri–Chromites. Int J Sci Res Physic Appl Sci. 2018;6:32-6.
Chamundeeswari M, Jeslin J, Verma ML. Nanocarriers for drug delivery applications. Environ Chem Lett. 2019;17(2):849-65.
Dahman Y. Nanotechnology and Functional Materials for Engineers. Amsterdam. Elsevier. 2017.
Vila A, Sánchez A, Janes K, Behrens I, Kissel T, Jato JLV, et al. Low molecular weight chitosan nanoparticles as new carriers for nasal vaccine delivery in mice. Eur J Pharm Biopharm. 2004;57(1):123-31.
Mortazavi SAR, Rezaei MA. Preparation and evaluation of diphtheria toxoid-containing microspheres. Ir J Pharm Sci. 2004; 3(3): 133-143.
_||_
.1 Rafiqi SI, Kumar S, Zehra A, Kumar D, Jain S, Sethi K, et al. Nanovaccinology: Dawn of biomimetic vaccine carriers. J Entomol Zool Stud. 2017; 5(2): 795-802 2017.
Apostólico JdS, Lunardelli VAS, Coirada FC, Boscardin SB, Rosa DS. Adjuvants: classification, modus operandi, and licensing. J Immunol Res. 2016;2016.
Prashant CK, Kumar M, Dinda AK. Nanoparticle based tailoring of adjuvant function: the role in vaccine development. J Biomed Nanotechnol. 2014;10(9):2317-31.
Tomljenovic L, Shaw C. Mechanisms of aluminum adjuvant toxicity and autoimmunity in pediatric populations. Lupus. 2012;21(2):223-30.
Principi N, Esposito S. Aluminum in vaccines: Does it create a safety problem? Vaccine. 2018;36(39):5825-31.
Esposito S, Mastrolia MV, Prada E, Pietrasanta C, Principi N. Vaccine administration in children with chronic kidney disease. Vaccine. 2014;32(49):6601-6.
Chen Y-C, Cheng H-F, Yang Y-C, Yeh M-K. Nanotechnologies applied in biomedical vaccines. Micro and Nanotechnologies for Biotechnology. Stanciu SG. London. IntechOpen. (2016): 84-105.
Lung P, Yang J, Li Q. Nanoparticle formulated vaccines: opportunities and challenges. Nanoscale. 2020;12(10):5746-63.
Ching SH, Bansal N, Bhandari B. Alginate gel particles–A review of production techniques and physical properties. Crit Rev Food Sci Nutr. 2017;57(6):1133-52.
Rajaonarivony M, Vauthier C, Couarraze G, Puisieux F, Couvreur P. Development of a New Drug Carrier Made from Alginate. J Pharm Sci. 1993;82(9):912-7.
Matsumoto H, Haniu H, Komori N. Determination of Protein Molecular Weights on SDS-PAGE. In: Kurien BT, Scofield RH, editors. Electrophoretic Separation of Proteins: Methods and Protocols. New York, Springer. (2019): 101-5.
Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976;72(1):248-54.
Rezaei MA, Mortazavi S, Mohammadpour DN, Zou AH, Alonso M. Preparation and in-vitro evaluation of sodium alginate microspheres containing diphtheria toxoid as new vaccine delivery. Arch Razi Inst. 2008; (2):19-28. 14. Papadimitriou S, Bikiaris D, Avgoustakis K, Karavas E, Georgarakis M. Chitosan nanoparticles loaded with dorzolamide and pramipexole. Carbohydr Polym. 2008;73(1):44-54.
Draget KI, Taylor C. Chemical, physical and biological properties of alginates and their biomedical implications. Food Hydrocoll. 2011;25(2):251-6.
Silva CM, Ribeiro AJ, Figueiredo IV, Gonçalves AR, Veiga F. Alginate microspheres prepared by internal gelation: Development and effect on insulin stability. Int J Pharm. 2006;311(1-2):1-10.
Alfatama M, Lim LY, Wong TW. Alginate–C18 conjugate nanoparticles loaded in tripolyphosphate-cross-linked chitosan–oleic acid conjugate-coated calcium alginate beads as oral insulin carrier. Mol Pharm. 2018;15(8):3369-82.
Ghaderinia P, Shapouri R. Assessment of immunogenicity of alginate microparticle containing Brucella melitensis 16M oligo polysaccharide tetanus toxoid conjugate in mouse. Banat's J Biotechnol. 2017;8(16):83-92.
Azimi S, Safari Zanjani L. Immunization against Pseudomonas aeruginosa using Alg-PLGA nano-vaccine. Ir J Basic Med Sci. 2021; 476-482.
Pandey R, Khuller G. Polymer based drug delivery systems for mycobacterial infections. Curr Drug Deliv. 2004;1(3):195-201.
Lin S-F, Chen Y-C, Chen R-N, Chen L-C, Ho H-O, Tsung Y-H, et al. Improving the stability of astaxanthin by microencapsulation in calcium alginate beads. PLoS One. 2016;11(4):e0153685.
Tsai S, Ting Y. Synthesize of alginate/chitosan bilayer nanocarrier by CCD-RSM guided co-axial electrospray: A novel and versatile approach. Food Res Int. 2019;116:1163-72.
González Ferreiro M, Tillman L, Hardee G, Bodmeier R. Characterization of alginate/ poly-L-lysine particles as antisense oligonucleotide carriers. Int J Pharm. 2002;239(1-2):47-59.
Sarei F, Dounighi NM, Zolfagharian H, Khaki P, Bidhendi SM. Alginate nanoparticles as a promising adjuvant and vaccine delivery system. Indian J Pharm Sci. 2013;75(4):442.
Dobakhti F, Rahimi F, Dehpour AR, Taghikhani M, Ajdary S, Rafiei S, et al. Stabilizing effects of calcium alginate microspheres on Mycobacterium bovis BCG intended for oral vaccination. J Microencapsul. 2006;23(8):844-54.26. Zimet P, Mombrú ÁW, Faccio R, Brugnini G, Miraballes I, Rufo C, et al. Optimization and characterization of nisin-loaded alginate-chitosan nanoparticles with antimicrobial activity in lean beef. LWT. 2018;91:107-16.
Baghbani F, Moztarzadeh F, Mohandesi JA, Yazdian F, Mokhtari-Dizaji M, Hamedi S. Formulation design, preparation and characterization of multifunctional alginate stabilized nanodroplets. Int J Biol Macromol. 2016;89:550-8.
Goudarzi HR, Mokarram A, Noofeli M, Shirvan A, Saadati M. Preparation and evaluation of alginate nanoparticles containing pertussis toxin as a particulate delivery system. Int J Adv Biotechnol Res. 2016;7(2):558-64.
Woitiski CB, Veiga F, Ribeiro A, Neufeld R. Design for optimization of nanoparticles integrating biomaterials for orally dosed insulin. Eur J Pharm Biopharm. 2009;73(1):25-33.
Woitiski CB, Neufeld RJ, Ribeiro AJ, Veiga F. Colloidal carrier integrating biomaterials for oral insulin delivery: influence of component formulation on physicochemical and biological parameters. Acta Biomater. 2009;5(7):2475-84.
31.Dórea JG. Exposure to mercury and aluminum in early life: developmental vulnerability as a modifying factor in neurologic and immunologic effects. Int J Env Res Public Health. 2015;12(2):1295-313.
Tarhini M, Benlyamani I, Hamdani S, Agusti G, Fessi H, Greige-Gerges H, et al. Protein-based nanoparticle preparation via nanoprecipitation method. Materials. 2018;11(3):394.
Moradi Bidhendi S, Zolfagharian H, Mohamadpour Dounighi N, Saraei F, Khaki P, Inanlou F. Design and evaluate alginate nanoparticles as a protein delivery system. Arch Razi Inst. 2013;68(2):139-46.
Ferreiro MG, Tillman L, Hardee G, Bodmeier R. Characterization of alginate/poly-L-lysine particles as antisense oligonucleotide carriers. Int J Pharm. 2002;239(1-2):47-59.
Modi K, Bhalodia J, Pathak T, Raval P, Pansara P, Vasoya N, et al. Bimodal to Unimodal Particle Size Distribution Transformation in Nanocrystalline Cobalt–Ferri–Chromites. Int J Sci Res Physic Appl Sci. 2018;6:32-6.
Chamundeeswari M, Jeslin J, Verma ML. Nanocarriers for drug delivery applications. Environ Chem Lett. 2019;17(2):849-65.
Dahman Y. Nanotechnology and Functional Materials for Engineers. Amsterdam. Elsevier. 2017.
Vila A, Sánchez A, Janes K, Behrens I, Kissel T, Jato JLV, et al. Low molecular weight chitosan nanoparticles as new carriers for nasal vaccine delivery in mice. Eur J Pharm Biopharm. 2004;57(1):123-31.
Mortazavi SAR, Rezaei MA. Preparation and evaluation of diphtheria toxoid-containing microspheres. Ir J Pharm Sci. 2004; 3(3): 133-143.
