ﺑﺮرﺳﯽ ﺑﯿﺎن ژن BAX در مخچه موش‌های صحرایی کلستاتیک تجربی، ﻣﺘﻌﺎﻗﺐ اﺛﺮ داروی ﻧﻮرواﯾﺪ
الموضوعات :افسانه کریمی آنچه 1 , مهرداد هاشمی 2 , محمد ناصحی 3
1 - دانشجو
2 - گروه ژنتیک ،دانشکده علوم نوین ،دانشگاه آزاد اسلامی واحد علوم پزشکی تهران ، تهران ،ایران
3 - گروه فیزیولوژی جانوری ،گروه زیست شناسی،دانشکده علوم نوین ،دانشگاه آزاد اسلامی واحد علوم پزشکی تهران ،تهران ،ایران
الکلمات المفتاحية: واژگان کلیدی: ", کلستاز", , ", ژنBax", , ", نوراید", , ", مخچه", , ", آپوپتوز", ,
ملخص المقالة :
چکیده: سابقه و هدف: کلستاز ازجمله بیماری‌های کبدی است که ازلحاظ عملکردی با کاهش یا قطع جریان صفرا همراه می‌باشد و باعث تجمع اسیدهای صفراوی در خون می‌شود، در صورت عدم درمان، کلستاز سبب آسیب به اندام‌های مختلف می‌گردد. در این تحقیق تأثیر کلستاز بر بیان ژن Bax که درروند مرگ برنامه‌ریزی‌شده‌ی سلول (آپوپتوز) مؤثراست، بررسی شد. روش بررسی: تعداد ۱۶ سر رت نر به ۴ گروه تقسیم شدند: گروه کنترل یا شاهد، گروه BDL: در این گروه مجرای صفراوی به شکل مصنوعی بسته شد، گروه BDL نوراید: در این گروه علاوه بربستن مجرای صفراوی، رت ها با داروی نوراید نیز تیمار شدند، گروه شم نوراید: در این گروه علاوه بر اینکه رت ها تحت استرس جراحی قرار گرفتند داروی نوراید نیز دریافت نمودند. سپس مخچه‌ی رت ها خارج شد. پس از استخراج RNA و سنتز cDNA، بیان ژن‌ها با فن‌آوری Real Time PCR موردبررسی قرار گرفت. یافته‌ها: BDL باعث افزایش بیان ژن Bax و استفاده از داروی نوراید باعث کاهش بیان ژن Bax در ناحیه‌ی مخچه رت ها شد. نتیجه‌گیری: تغییرات ایجادشده توسط کلستاز در جهت افزایش آپوپتوز در ناحیه‌ی مخچه‌ی رت ها می‌باشد و استفاده از داروی نوراید می‌تواند باعث کاهش آپوپتوز در مخچه‌ی رت ها شود.
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